Research on four-bar linkage trajectory synthesis using extreme gradient boosting and genetic algorithm

Wang Jianping,Chen Boyan,Wang Youchao,Pu Dexi,Jia Xiaoyue 한국CDE학회 2024 Journal of computational design and engineering Vol.11 No.2

The current study on the synthesis problems of four-bar mechanism trajectories primarily relies on establishing a numerical atlas based on trajectory characteristics and employing neural networks to synthesize mechanism parameters. However, this approach has several shortcomings, including a vast database, inefficient retrieval, and challenges in maintaining accuracy. This paper presents a method for synthesizing a trajectory-generation mechanism that combines the extreme gradient boosting (XGBoost) algorithm with a genetic algorithm (GA). The purpose is to synthesize, based on a particular trajectory, the dimensions and installation position parameters of a four-bar mechanism. The paper classifies the trajectories according to their shape features and geometric center placements, thereby improving the accuracy of the XGBoost model for synthesizing mechanisms. The XGBoost algorithm is employed to synthesize the basic dimensional parameters for the mechanism, with the relative slopes of trajectories as input features. The synthesized basic dimensional parameters are turned into parameters for the actual mechanism by researching the scaling, translation, and rotation relationships between mechanisms and the trajectories they generate. The accuracy of the generated trajectories from the synthesized mechanism can be improved by applying GA to optimize the mechanism parameters. Five comparative examples are provided in this research for the different scenarios of given trajectory curves and trajectory points. The effectiveness and accuracy of the proposed approach in this study are validated in comparison to existing research methods by comparing errors between the generated trajectories and the given trajectories.

Frequency-Domain Transformation Approaches to Develop the d-q Synchronous-Frame Models of Three-Phase Symmetrical Networks and Applications in Modeling of PWM Power Converters

Xiaoyu Wang,Jinjun Liu,Yuji Meng,Jinku Hu,Chang Yuan 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

Although state-space averaging small-signal modeling techniques are successfully established in DC-DC converters and three-phase sinusoidal PWM converters, these approaches are limited only to low-order systems, and the modeling process is very complex. This paper proposes two equivalent approaches in frequency domain to develop the d-q synchronous-frame models from stationary-frame models for arbitrary three-phase symmetrical circuits. The first modeling approach is illustrated with frequency transformation, by which the target synchronous-frame models could be easily obtained by frequency transformation of the prototype stationary-frame models. The second modeling approach is based on the reactive elements substitutions. These two approaches can be proven to be equivalent, and the first approach is suitable for input-output models, while the second approach is preferable for state-space models. As illustrative examples, these approaches are applied to develop the synchronous-frame models of large three-phase systems with PWM converters. They are proven valid with the comparison with current published models.

Frequency Characteristics of the d-q Synchronous-Frame Current Reference Generation Methods for Active Power Filter

Xiaoyu Wang,Jinjun Liu,Jinku Hu,Yuji Meng,Chang Yuan 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

The d-q harmonics detection algorithms are dominant methods to generate current reference for active power filter (APF). They are often processed in synchronous frame and time domain. This paper researches the frequency characteristics of d-q synchronous transformation which are quite related to the design of close loop regulator and analysis of the performance. Intuitively, the synchronous transformation is explained with amplitude modulation (AM) in this paper. Then the synchronous filter is proven as time-invariant linear transformation and modeled with transfer functions in stationary frame and frequency domain. The frequency-domain analytical models imply that the synchronous transformation has an equivalent effect of frequency transformation, with which the synchronous filter achieves a band pass filter from the low pass filter in synchronous frame. To simplify the analytical model, an instantaneous positive/negative sequence frame is proposed as an expansion of symmetrical components theory. Based on these frequency analytical models, the synchronous filter is compared with the traditional filters in stationary frame. Further more, the d-q harmonics detection methods are improved to get rid of the inherent coupling of synchronous transformation. Typical examples are given to verify previous analysis and comparison. Simulation and experimental results are also given.

ssc-miR-185 targets cell division cycle 42 and promotes the proliferation of intestinal porcine epithelial cell

Wang, Wei,Wang, Pengfei,Xie, Kaihui,Luo, Ruirui,Gao, Xiaoli,Yan, Zunqiang,Huang, Xiaoyu,Yang, Qiaoli,Gun, Shuangbao Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5

Objective: microRNAs (miRNAs) can play a role in a variety of physiological and pathological processes, and their role is achieved by regulating the expression of target genes. Our previous high-throughput sequencing found that ssc-miR-185 plays an important regulatory role in piglet diarrhea, but its specific target genes and functions in intestinal porcine epithelial cell (IPEC-J2) are still unclear. We intended to verify the target relationship between porcine miR-185 and cell division cycle 42 (CDC42) gene in IPEC-J2 and to explore the effect of miR-185 on the proliferation of IPEC-J2 cells. Methods: The TargetScan, miRDB, and miRanda software were used to predict the target genes of porcine miR-185, and CDC42 was selected as a candidate target gene. The CDC42-3' UTR-wild type (WT) and CDC42-3'UTR-mutant type (MUT) segments were successfully cloned into pmirGLO luciferase vector, and the luciferase activity was detected after co-transfection with miR-185 mimics and pmirGLO-CDC42-3'UTR. The expression level of CDC42 was analyzed using quantitative polymerase chain reaction and Western blot. The proliferation of IPEC-J2 was detected using cell counting kit-8 (CCK-8), methylthiazolyldiphenyl-tetrazolium bromide (MTT), and 5-ethynyl-2'-deoxyuridine (EdU) assays. Results: Double enzyme digestion and sequencing confirmed that CDC42-3'UTR-WT and CDC42-3'UTR-MUT were successfully cloned into pmirGLO luciferase reporter vector, and the luciferase activity was significantly reduced after co-transfection with miR-185 mimics and CDC42-3'UTR-WT. Further we found that the mRNA and protein expression level of CDC42 were down-regulated after transfection with miR-185 mimics, while the opposite trend was observed after transfection with miR-185 inhibitor (p<0.01). In addition, the CCK-8, MTT, and EdU results demonstrated that miR-185 promotes IPEC-J2 cells proliferation by targeting CDC42. Conclusion: These findings indicate that porcine miR-185 can directly target CDC42 and promote the proliferation of IPEC-J2 cells. However, the detailed regulatory mechanism of miR-185/CDC42 axis in piglets' resistance to diarrhea is yet to be elucidated in further investigation.

Frequency Characteristics of the Synchronous-Frame Based D-Q Methods for Active Power Filters

WANG Xiaoyu,LIU Jinjun,HU Jinku,MENG Yuji,YUAN Chang 전력전자학회 2008 JOURNAL OF POWER ELECTRONICS Vol.8 No.1

The d-q harmonic detecting algorithms are dominant methods to generate current references for active power filters (APF). They are often implemented in the synchronous frame and time domain. This paper researches the frequency characteristics of d-q synchronous transformations, which are closely related to the analysis and design issues of control system. Intuitively, the synchronous transformation is explained with amplitude modulation (AM) in this paper. Then, the synchronous filter is proven to be a time-invariant and linear system, and its transfer function matrix is derived in the stationary frames. These frequency-domain models imply that the synchronous transformation has an equivalent effect of frequency transformation. It is because of this feature, the d-q method achieves band-pass characteristics with the low pass filters in the synchronous frame at run time. To simplify these analytical models, an instantaneous positive-negative sequence frame is proposed as expansion of traditional symmetrical components theory. Furthermore, the synchronous filter is compared with the traditional bind-pass filters based on these frequency-domain analytical models. The d-q harmonic detection methods are also improved to eliminate the inherent coupling effect of synchronous transformation. Typical examples are given to verify previous analysis and comparison. Simulation and experimental results are also provided for verification.

Transfer Pricing and PE taxation in China

Wang Xiaoyue 한국국제조세협회 2008 조세학술논집 Vol.24 No.2

Blood lipids and all-cause mortality among old people: the Chinese Longitudinal Healthy Longevity Survey 2008-2018

Wang Rongxi,Yu Xiaoyue,Wang Zhiqiang,Liu Yujie,Chen Hui,Liu Shangbin,XU CHENG,Chen Yingjie,Xia Danni,Ge Xin,Chang Ruijie,Xu Gang,Xiang Mi,Wang Ying,Shen Tian,Hu Fan,Cai Yong 한국역학회 2022 Epidemiology and Health Vol.44 No.-

OBJECTIVES: Proper blood lipid levels are essential for survival in older adults, but inconsistent relationships have been reported between blood lipids and all-cause mortality in the elderly. METHODS: This retrospective longitudinal study analyzed data from 1,067 Chinese older adults enrolled in the Chinese Longitudinal Healthy Longevity Survey collected in 2008 and followed up until death or December 31, 2018. The outcome was allcause mortality. Multivariate Cox regression analyses were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) with stratification by age (60-80, 80-100, or ≥ 100 years) for further analysis. The survival probability according to lipid profile quartiles was calculated using Kaplan-Meier curves and the log-rank test. RESULTS: The participants’ mean age was 84.84 years, and 57.0% were female. In total, 578 individuals died, and 277 were lost to follow-up. The mean total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were higher among those who died than among those who survived. Participants in the second HDL-C quartile and the highest LDL-C and triglyceride (TG) quartiles had 28% higher, 23% lower, and 49% lower risks of all-cause mortality, respectively. After further adjustment, the associations remained except for HDL-C, and additional associations were observed between all-cause mortality and the third TC and LDL-C quartiles and the second TG quartile (HR, 1.44; 95% CI, 1.01 to 2.06; HR, 0.68; 95% CI, 0.49 to 0.94; HR, 0.79; 95% CI, 0.62 to 0.99, respectively). CONCLUSIONS: Older adults should maintain an LDL-C level of 1.91-2.47 mmol/L and a TG level of no less than 1.66 mmol/L.

HOW MUCH DOES PRODUCT-HARM CRISIS MATTER TO OVERALL SATISFACTION WITH THE SUPPLIERS?

Xiaoyu Wang,Simon Shufeng Xiao 동중앙아시아경상학회 2013 한몽경상학회 학술대회 Vol.2013 No.2

Structural understanding of the transport cycle of glutamate transporters

Xiaoyu Wang,SeCheol Oh 한국구조생물학회 2020 Biodesign Vol.8 No.4

Since the determination of the first crystal structure of a glutamate transporter homolog GltPh 16 years ago, the structural biology of glutamate transporter has progressed remarkably. To understand the complicated transport cycle of ion-coupled glutamate transport, each state’s high-resolution structural information in the transport cycle is essential. Many of the structural information and insights about the sodium-coupled symport mechanism were gained from structural studies of prokaryotic homologs sodium-aspartate symporters, GltPh and GltTk. Moreover, further insights were gained from recently unveiled structures of mammalian glutamate transporters (EAATs). Here we review GltPh, GltTk, and mammalian glutamate transporters’ structures and discuss how they help us to understand the ion-coupled transport mechanism in glutamate transporters.

Identification of a Novel CSNK2A1-PDGFRB Fusion Gene in a Patient with Myeloid Neoplasm with Eosinophilia

Xiaoyu Xu,Qiongyu Lu,Zheng Wang,Ping Cai,Zhao Zeng,Ling Zhang,Man Wang,Liang Ma,Changgeng Ruan,Suning Chen 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3

Platelet-derived growth factor receptor beta (PDGFRB) rearrangements play an important role in the pathogenesis of eosinophilia-associated myeloid/lymphoid neoplasms. Up to now, more than 70 PDGFRB fusions have been identified. Here, a novel PDGFRB fusion gene CSNK2A1-PDGFRB has been identified in myeloproliferative neoplasm (MPN) with eosinophilia by RNA-sequencing, which has been verified by reverse transcription polymerase chain reaction and Sanger sequencing. The new PDGFRB fusion partner gene CSNK2A1 encoded one of the two catalytic subunit of casein kinase II (CK2). To our knowledge, this is the first report on the involvement of CSNK2A1 in fusion genes, especially fusion with another kinase PDGFRB in MPN. In addition, the CSNK2A1-PDGFRB fusion retained the entire kinase domain of PDGFRB and response to imatinib at low concentration. The patient with CSNK2A1-PDGFRB was sensitive to imatinib treatment and acquired sustained complete remission.