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聚焦动手实践,培养创新能力—初中物理实验教学中培养学生创新能力的策略
毛小龙(Xiaolong Mao) YIXIN 출판사 2024 Education Reform and Teaching Research Vol.1 No.2
In the new era, cultivating innovative talents has become the consensus of teachers in different classes. Under the background of educational reform, junior high school physics teachers should not only pay attention to students’ basic knowledge, but also consciously cultivate students’ innovative ability when carrying out experimental teaching. By designing teaching experiments and guiding students to practice, students can accumulate experience and develop their abilities and thinking, so that they can gradually grow into talents with innovative ability. This paper studies the strategies of cultivating students' innovative ability in junior high school physics experiment teaching, and points out that teachers need to change their ideas, innovate teaching methods and pay attention to students’ experience, so as to create a new space for students' innovative ability development. 新时期下,培养创新型人才成为不同学段教师的共识。在教育改革背景下,初中物理教师在开展实验教学时,不仅要关注学生基础知识的掌握,同时也要有意识地培养学生创新能力。通过设计教学实验、引导学生实践,使其积累经验,获得能力与思维的发展,从而使其逐渐成长为具备创新能力的人才。本文就初中物理实验教学中培养学生创新能力的策略进行研究,指出教师需转变思想、创新教学方法、关注学生体验等,为学生打造创新能力发展的全新空间。
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Mao Jianxin,Wang Di,Wang Dong,Wu Qi,Shang Qiliang,Gao Chu,Wang Huanbo,Wang Han,Du Mu,Peng Pandi,Jia Haoruo,Xu Xiaolong,Wang Jie,Yang Liu,Luo Zhuojing 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Mitochondrial dysfunction plays a major role in the development of intervertebral disc degeneration (IDD). Sirtuin 5 (SIRT5) participates in the maintenance of mitochondrial homeostasis through its desuccinylase activity. However, it is still unclear whether succinylation or SIRT5 is involved in the impairment of mitochondria and development of IDD induced by excessive mechanical stress. Our 4D label-free quantitative proteomic results showed decreased expression of the desuccinylase SIRT5 in rat nucleus pulposus (NP) tissues under mechanical loading. Overexpression of Sirt5 effectively alleviated, whereas knockdown of Sirt5 aggravated, the apoptosis and dysfunction of NP cells under mechanical stress, consistent with the more severe IDD phenotype of Sirt5 KO mice than wild-type mice that underwent lumbar spine instability (LSI) surgery. Moreover, immunoprecipitation-coupled mass spectrometry (IP-MS) results suggested that AIFM1 was a downstream target of SIRT5, which was verified by a Co-IP assay. We further demonstrated that reduced SIRT5 expression resulted in the increased succinylation of AIFM1, which in turn abolished the interaction between AIFM1 and CHCHD4 and thus led to the reduced electron transfer chain (ETC) complex subunits in NP cells. Reduced ETC complex subunits resulted in mitochondrial dysfunction and the subsequent occurrence of IDD under mechanical stress. Finally, we validated the efficacy of treatments targeting disrupted mitochondrial protein importation by upregulating SIRT5 expression or methylene blue (MB) administration in the compression-induced rat IDD model. In conclusion, our study provides new insights into the occurrence and development of IDD and offers promising therapeutic approaches for IDD.
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Yunyun Wei,Jianfeng Qiu,Ziqiang Han,Xuanyi Wang,Hui Zhang,Xinya Hou,Xiangwei Lv,Xiaolong Mao 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background For some ICU patients, an artificial airway must be established with an endotracheal tube, but Candida albicans can easily adhere to the tube surface and form a biofilm, leading to potentially life threatening fungal infections. Therefore, it is urgent to prevent and reduce C. albicans infections introduced by the endotracheal tube. However, there are few antifungal drugs effective against C. albicans, and each of these drugs may have adverse effects on human cells. Saccharomyces boulardii is regarded as an alternative strategy to inhibit the adhesion of C. albicans, but it is affected by environmental stress. We hypothesized that it is feasible to strengthen the antagonistic ability of S. boulardii via encapsulating and genetically modification. Methods In this study, a bioactive material carrying the overexpressed MCP1 gene of Saccharomyces boulardii was constructed based on one-step photo-crosslinking. This material achieved spatial growth control of S. boulardii by encapsulating each S. boulardii cell within a hydrogel pore. The bioactive material was coated on an endotracheal tube and tested for its ability to inhibit the adhesion of C. albicans. Additionally, the material’s antagonistic activity towards C. albicans was evaluated by detecting intracellular Adenosine-triphosphate content, reactive oxygen species level and the activity of antioxidative enzymes. Tissue invasion experiment was executed to further evaluate the antiadhesion ability of S. boulardii bio-coating. Results Encapsulating the overexpression of MCP1 by S. boulardii in hydrogel pores enhanced the viability of probiotics in the presence of high salt and oxidation stress. When used as the coating of an endotracheal tube, the S. boulardii bioactive material efficiently inhibited the adhesion of C. albicans by impairing the activities of superoxide dismutase and catalase and disturbing mitochondrial functions. In vivo, the S. boulardii bioactive material coating displayed good biocompatibility and reduced the host tissue invasion and virulence of C. albicans. Conclusions The integration of genetic modification and immobilization model breaks the bottleneck of previous application of microorganisms, and provides a new way to prevent fungal infections introduced by endotracheal tubes.
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