Selection of High Laccase-Producing Coriolopsis gallica Strain T906: Mutation Breeding, Strain Characterization, and Features of the Extracellular Laccases

( Xiaoli Xu ),( Lei Feng ),( Zhenya Han ),( Sishi Luo ),( Ai`min Wu ),( Jun Xie ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.9

Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. gallica TCK was treated with N-methyl-N-nitro Nnitrosoguanidine and ultraviolet light. Among the mutants isolated, T906 was found to be a high-production strain of laccases. The mutant strain T906 was stabilized via dozens of passages, and the selected ones were further processed for optimization of metallic ion, inducers, and nutritional requirements, which resulted in the optimized liquid fermentation medium MF9. The incubation temperature and pH were optimized to be 30°C and 4.5, respectively. The mutant strain T906 showed 3-times higher laccase activity than the original strain TCK under optimized conditions, and the maximum laccase production (303 U/ml) was accomplished after 13 days. The extracellular laccase isoenzyme 1 was purified and characterized from the two strains, respectively, and their cDNA sequence was determined. Of note, the laccase isoenzyme 1 transcription levels were overtly increased in T906 mycelia compared with values obtained for strain TCK. These findings provide a basis for C. gallica modification for the production of high laccase amounts.

125I Seed Permanent Implantation as a Palliative Treatment for Stage III and IV Hypopharyngeal Carcinoma

Lei Li,Jie Yang,Xiaojiang Li,Xiaoli Wang,Yanxin Ren,Jimin Fei,Yan Xi,Ruimei Sun,Jing Ma 대한이비인후과학회 2016 Clinical and Experimental Otorhinolaryngology Vol.9 No.3

Objectives. The aim of this study was to investigate the feasibility and safety of percutaneous 125I seed permanent implantation for advanced hypopharyngeal carcinoma from toxicity, tumor response, and short-term outcome. Methods. 125I seeds implant procedures were performed under computed tomography for 34 patients with advanced hypopharyngeal carcinoma. We observed the local control rate, overall survival, and acute or late toxicity rate. Results. In the 34 patients (stage III, n=6; stage IV, n=28), the sites of origin were pyriform sinus (n=29) and postcricoid area (n=5). All patients also received one to four cycles of chemotherapy after seed implantation. The post-plan showed that the actuarial D90 of 125I seeds ranged from 90 to 158 Gy (median, 127 Gy). The mean follow-up was 12.3 months (range, 3.4 to 43.2 months). The local control was 2.1–31.0 months with a median of 17.7 months (95% confidence interval [CI], 13.4 to 22.0 months). The 1-, 2-, and 3-year local controls were 65.3%, 28.6%, and 9.5% respectively. Twelve patients (35%) died of local recurrence, fourteen patients (41%) died of distant metastases, and three patients (9%) died of recurrence and metastases at the same time. Five patients (15%) still survived to follow-up. At the time of analysis, the median survival time was 12.5 months (95% CI, 9.5 to 15.4 months). The 1-, 2-, and 3-year overall survival rates were 55.2%, 20.3%, and 10.9%, respectively. Five patients (15%) experienced grade 3 toxic events and nine patients (26%) have experienced grade 2 toxic events. Conclusion. This review shows relatively low toxicity for interstitial 125I seed implantation in the patients with advanced stage hypopharyngeal cancer. The high local control results suggest that 125I seed brachytherapy implant as a salvage or palliative treatment for advanced hypopharyngeal carcinoma merit further investigation.

Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling

Lei Du,Lina Wang,Hong Yang,Jianping Duan,Jianming Lai,Wei Wu,Shaohua Fan,Xiaoli Zhi 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.12

Purpose: The purpose of this study was to investigate the influences of sex comb on midleg like-2 (SCML2) on hepatocellular carcinoma (HCC) and potentially related mechanisms. Materials and Methods: SCML2 expression in tumor tissues and cells was analyzed using the TCGA database and/or qRT-PCR. The proliferation of HCC cells was detected by CCK-8, colony formation, and EdU assays. The migration and invasion of HCC cells were detected by transwell and wound healing assays. Apoptosis of HCC cells was determined by flow cytometry. Additionally, qRT-PCR and Western blot were used to detect the expression of SCML2 and Wnt/β-catenin/epithelial–mesenchymal transition (EMT) signaling. A xenograft model in mice was established to verify the in vitro findings. Results: We found that SCML2 was highly expressed in HCC tissues and cells and that high expression of SCML2 was correlated with poor prognosis in HCC patients. SCML2 overexpression promoted proliferation, invasion, and migration and repressed apoptosis of HCC cells. The reverse results were obtained in SCML2-silenced cells. Further, we found that SCML2 activated the Wnt/β-catenin/EMT pathway. SCML2 silencing reduced the protein levels of Wnt3a, β-catenin, N-cadherin, Vimentin, and Snail and enhanced E-cadherin protein expression both in vivo and in vitro. Conclusion: SCML2 silencing inhibits the proliferation, migration, and invasion of HCC cells by regulating the Wnt/β-catenin/EMT pathway.

Effects of lycopene on abdominal fat deposition, serum lipids levels and hepatic lipid metabolism-related enzymes in broiler chickens

Wan Xiaoli,Yang Zhengfeng,Ji Haoran,Li Ning,Yang Zhi,Xu Lei,Yang Haiming,Wang Zhiyue 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.3

Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens. Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively. Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05). Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism. Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens.Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively.Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05).Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism.

Facial Synthesis of Zn-Doped Fe3O4 with Enhanced Electromagnetic Wave Absorption Performance in S and C Bands

Zhenfeng Liu,Honglong Xing,Lei Wang,Dexin Tan,Ying Gan,Xiaoli Ji,Guocai Xu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.8

In this study, Zn-doped Fe3O4 nanoparticles were successfully synthesized by a facile solvothermal method in the presence of sodium dodecyl sulfate (SDS). The morphology, magnetic properties and electromagnetic wave absorbing properties of these materials were characterized. Results showed that Zn2+ played a significant role in the formation of Zn-doped Fe3O4. With the protection of SDS, highly dispersed Fe3O4 nanoparticles were obtained. The nanoparticle size decreased after Zn2+ doping, and the dispersity deteriorated with increasing Zn2+ doping concentration. Zndoped Fe3O4 exhibited excellent electromagnetic wave absorbing property, which resulted in magnetic loss and dielectric loss at an appropriate doping concentration. The minimum reflection loss (RL) was approximately -27.2 dB at 16.9 GHz. As the coating layer thickness increased to 4.0 mm, the bandwidth was approximately 5.0 GHz corresponding to RL below -10 dB, which nearly covered the entire S band (2–4 GHz) and C band (4–8 GHz). The peak frequency of RL and the number of peaks matched the quarter-wave thickness criteria. It was believed that the Zndoped Fe3O4 could be a potential electromagnetic wave absorbing material in S and C bands.

Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats

Mengyun Li,Wu-Qing OuYang,Xiaoli Wu,Yin Zheng,Yunpeng Wei,Lei An 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.3

Kinetin (Kn) is a cytokinin growth factor that exertsseveral anti-aging and antioxidant effects on cells andorgans. To investigate the mechanism underlying apoptoticevents in aging cells induced by D-galactose (D-gal), weexamined the effect of Kn delivered via nuchalsubcutaneous injection on D-gal-induced aging andapoptosis in rats. Our results showed that interleukin (IL)-2levels and mitochondrial membrane potential (Δ Ψ m) weredecreased by Kn in aging rats while IL-6 production andapoptosis increased. In addition, the expression ofanti-apoptotic Bcl-2 was low while that of Bax was high inthe aging group. After treated with Kn, compared withaging group, there showed obvious difference in Kn groupwith elevated IL-2, proliferation index, Bcl-2, Δ Ψ m anddecreased IL-6 and Bax in splenic lymphocyte. Based onthese results, we concluded that Kn can effectively protectthe rat spleen from aging, apoptosis, and atrophy.

Electrochemical method for determining the solubility of impurities in lead-bismuth eutectic using molten salt electrolytes

Yin Taiqi,Zhang Yongcheng,Zhang Lei,Bo Tao,Tan Xiaoli,Shi Weiqun 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.10

Traditional physical methods for measuring solubility were often affected by viscosity, density, and surface tension. In this study, we selected appropriate electrolytes to determine the solubility of impurities in the leadbismuth circuit at reactor operating temperatures using electrochemical methods. Taking the measurement of the solubility of impurity Ce in liquid Bi as an example, we demonstrated that electrochemical methods can accurately determine its solubility in the LiCl-KCl molten salt. Furthermore, the successful measurement of other impurities such as La, Ce, U, and Mn in lead-bismuth eutectic (LBE) using the designed system has confirmed the universality of this method.

Stable Amorphous Bis(diarylamino)biphenyl Derivatives as Hole-Transporting Materials in OLEDs

Zhanfeng Li,Zhaoxin Wu,Wen Fu,Dongdong Wang,Peng Liu,Bo Jiao,Xiaoli Lei,Guijiang Zhou,Yuying Hao 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.5

Stable bis(diarylamino)biphenyl derivatives N4,N4'-di(biphenyl-4-yl)-N4,N4'-bis(2- methylbiphenyl-4-yl)biphenyl-4,4'-diamine (TPD-Ph) and N4,N4'-bis(2',4'-difluoro- 2-methylbiphenyl-4-yl)-N4,N4'-bis(2',4'-difluorobiphenyl-4-yl)biphenyl-4,4'-diamine (TPD-(2,4)-F) were synthesized and characterized. High thermal stability (Td ≥ 480°C and Tg ≥ 108°C) in combination with reversible oxidation process render the both promising candidates as hole-transporting materials for organic light-emitting devices. The device with structure of ITO/TPD-Ph/Alq3/LiF/Al presented the highest current efficiency of 4.2 cd A−1 and good operational stability even under the high current density of 350 mA cm−2, which outperformed TPD or NPB-based devices.