Carboxymethylated chitosan alleviated oxidative stress injury in retinal ganglion cells via IncRNA-THOR/IGF2BP1 axis

Xiaoli Wu,Yingying Liu,Yun Ji 한국유전학회 2021 Genes & Genomics Vol.43 No.6

Background Glaucoma is an advanced nerve disorder described by the deterioration of axon and RGCs. CMCS has been previously used as an anti-apoptotic and anti-oxidant agent. Objective The current study aimed to explore the protective impact of CMCS against H2O2-induced injury in glaucoma in vitro. Methods The relative expression of lncRNA THOR and the protein expression of IGF2BP1 in H2O2-induced RGC-5 cells were detected by RT-PCR and western blot methods respectively. The cell viability was measured using MTT assay while apoptosis rate was measured by fow cytometry. Moreover, ROS level was measured using ROS assay kit. Furthermore, the relations between THOR and IGF2BP1 were determined by using RNA pull-down. Results The expression of THOR was reduced in H2O2-induced RGCs. Also, RGCs viability was inhibited while the level of ROS and cell apoptosis were enhanced. CMCS treatment considerably enhanced the expression of THOR and IGF2BP1 protein and cell viability but reduced ROS level and cell apoptosis. Moreover, IGF2BP1 protein was positively regulated by lncRNA THOR. CMCS protected the RGCs from oxidative stress via regulating lncRNA THOR/IGF2BP1. Conclusion CMCS enhanced the cell viability and reduced the cell apoptosis and ROS level and protected RGCs from oxidative stress via lncRNATHOR/IGF2BP1 pathway, potentially suggesting a new therapeutic strategy for the treatment of glaucoma.

Fast Ambiguity Resolution using Galileo Multiple Frequency Carrier Phase Measurement

Shengyue Ji,Wu Chen,Chunmei Zhao,Xiaoli Ding,Yongqi Chen 한국항해항만학회 2006 한국항해항만학회 학술대회논문집 Vol.1 No.-

Rapid and high-precision positioning with a Global Navigation Satellite System (GNSS) is feasible only when very precise carrier-phase observations can be used. There are two kinds of mathematical models for ambiguity resolution. The first one is based on both pseudorange and carrier phase measurements, and the observation equations are of full rank. The second one is only based on carrier phase measurement, which is a rank-defect model. Though the former is more commonly used, the latter has its own advantage, that is, ambiguity resolution will be freed from the effects of pseudorange multipath. Galileo will be operational. One of the important differences between Galileo and current GPS is that Galileo will provide signals in four frequency bands. With more carrier-phase data available, frequency combinations with long equivalent wavelength can be formed, so Galileo will provide more opportunities for fast and reliable ambiguity resolution than current GPS. This paper tries to investigate phase only fast ambiguity resolution performance with four Galileo frequencies for short baseline. Cascading Ambiguity Resolution (CAR) method with selected optimal frequency combinations and LAMBDA method are used and compared. To validate the resolution, two tests are used and compared. The first one is a ratio test. The second one is lower bound success-rate test. The simulation test results show that, with LAMBDA method, whether with ratio test or lower bound success rate validation criteria, ambiguity can be fixed in several seconds, 8 seconds at most even when 1 sigma of carrier phase noise is 12 mm. While with CAR method, at least about half minute is required even when 1 sigma of carrier phase noise is 3 mm. It shows that LAMBDA method performs obviously better than CAR method.

Effects of lycopene on abdominal fat deposition, serum lipids levels and hepatic lipid metabolism-related enzymes in broiler chickens

Wan Xiaoli,Yang Zhengfeng,Ji Haoran,Li Ning,Yang Zhi,Xu Lei,Yang Haiming,Wang Zhiyue 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.3

Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens. Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively. Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05). Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism. Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens.Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively.Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05).Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism.

Establishment of Miscanthus sinensis with decreased lignin biosynthesis by Agrobacterium-mediated transformation using antisense COMT gene

Yoo, Ji Hye,Seong, Eun Soo,Ghimire, Bimal Kumar,Heo, Kweon,Jin, Xiaoli,Yamada, Toshihiko,Clark, Lindsay V.,Sacks, Erik J.,Yu, Chang Yeon Springer-Verlag 2018 Plant cell, tissue and organ culture Vol.133 No.3

Identifying genes for resistant starch, slowly digestible starch, and rapidly digestible starch in rice using genome-wide association studies

Ning Zhang,Maike Wang,Ji Fu,Yi Shen,Yi Ding,Dianxing Wu,Xiaoli Shu,Wenjian Song 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background The digestibility of starch is important for the nutritive value of staple food. Although several genes are responsible for resistant starch (RS) and slowly digestible starch (SDS), gaps persist concerning the molecular basis of RS and SDS formation due to the complex genetic mechanisms of starch digestibility. Objectives The objective of this study was to identify new genes for starch digestibility in rice and interprete the genetic mechanisms of RS and SDS by GWAS. Methods Genome-wide association studies were conducted by associating the RS and SDS phenotypes of 104 re-sequenced rice lines to an SNP dataset of 2,288,867 sites using a compressed mixed linear model. Candidate genes were identifed according to the position of the SNPs based on data from the MSU Rice Genome Annotation Project. Results Seven quantitative trait loci (QTLs) were detected to be associated with the RS content, among which the SNP 6 m1765761 was located on Waxy. Starch branching enzymes IIa (BEIIa) close to QTL qRS-I4 was detected and further identifed as a specifc candidate gene for RS in INDICA. Two QTLs were associated with SDS, and the LOC_Os09g09360 encoding lipase was identifed as a causal gene for SDS. Conclusions GWAS is a valid strategy to genetically dissect the formation of starch digestion properties in rice. RS formation in grains is dependent on the rice type; lipid might also contribute to starch digestibility and should be an alternative factor to improve rice starch digestibility.

Preparation, Microwave Absorption and Infrared Emissivity of Ni-doped ZnO/Al Powders by Coprecipitation Method in the GHz Range

Ruiwen Shu,Honglong Xing,Xiaoli Cao,Xiaoli Ji,Dexin Tan,Ying Gan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.4

In this work, Ni-doped ZnO/Al composites were prepared by a facile chemical co-precipitation method. The morphology and structure of the as-prepared composites were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS), respectively. It was found that the flakelike Al powders were successfully coated by Ni-doped ZnO nanoparticles with slight aggregation and Ni2+ was successfully doped into the crystal lattice of ZnO. Moreover, the effects of ZnO concentration and doped Ni concentration on the infrared emissivity of ZnO/Al composites at the waveband range of 8–14 µm were studied. The results showed that the ZnO/Al composites exhibited the lowest infrared emissivity of 0.34 with 50 wt.% ZnO concentration. Meanwhile, the electromagnetic parameters and microwave absorbing properties of Ni-doped ZnO/Al composites in the frequency range of 2–18 GHz were explored. Significantly, 12 mol.% Ni-doped ZnO/Al composites presented the lowest infrared emissivity of 0.37 and the maximum reflection loss reached -32.5 dB at 13.6 GHz with a thickness of 4.5 mm. The excellent microwave absorbing properties could be attributed to the good impedance match, crystal lattice defects and interfacial polarization. It was believed that the Ni-doped ZnO/Al composites could be used as potential infrared-microwave compatible stealth materials.

Enhanced Microwave Absorption Properties of CeO2 Nanoparticles Supported on Reduced Graphene Oxide

Qing Yin,Honglong Xing,Ruiwen Shu,Xiaoli Ji,Dexin Tan,Ying Gan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.5

In this work, reduced graphene oxide/CeO2 nanocomposites (RGO/CeO2) with two different RGO contents were synthesized using a facile one-step hydrothermal method, and the microwave absorption properties of RGO/CeO2 were investigated for the first time. Morphology and structure analysis shows that the CeO2 nanoparticles are uniformly dispersed on the RGO sheets with average size of 15 nm. The as-prepared RGO/CeO2 exhibits excellent microwave absorbability. An optimal reflection loss (RL) of -32 dB is found at 17 GHz with a coating layer thickness of 1.5 mm. The product with a coating layer thickness of only 2.0 mm shows a bandwidth of 4.3 GHz, corresponding to RL at -10 dB (90% of electromagnetic wave absorption). Compared with pristine RGO or pure CeO2 nanoparticles, the reported nanocomposites achieved both wider and stronger wave absorption in the frequency range of 2–18 GHz. The enhanced microwave absorption properties are attributed to the conductive loss and dielectric loss mainly caused by the higher oxygen vacancy concentration of CeO2 in RGO/CeO2, which is demonstrated by X-ray photoelectron spectroscopy. Moreover, multiple interfacial polarizations occurring in the multi-interfaces between CeO2 and RGO sheets may be beneficial to microwave absorption. RGO/CeO2 could be used as an attractive candidate for the new type of microwave absorptive materials.

Facial Synthesized Co-doped SnO2@Multi-Walled Carbon Nanotubes as an Efficient Microwave Absorber in High Frequency Range

Zhenfeng Liu,Honglong Xing,Ling Lin,Xiaoli Ji,Ziyao Shen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.10

Co-doped SnO2@multi-walled carbon nanotubes (MWCNTs) were fabricated by a one-pot hydrothermal process. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), high-resolution electron microscopy, and X-ray photoelectron spectroscopy (XPS) were employed to characterize the morphology and structure of the composites. XRD, FT-IR, and XPS analyses demonstrated that Co was doped into SnO2 lattice, no other impure phases were detected. Co-doped SnO2, with a uniform size of 4–6 nm, is coated on MWCNTs but separated from the nanotubes with some aggregation. The microwave absorption properties of Co-doped SnO2@MWCNTs were investigated at room temperature within 2–18 GHz. Results indicated that Co-doping concentration plays an important role in the microwave absorption capability of Co-doped SnO2@MWCNTs. The maximum reflection loss (RL) is 22.8 dB at 14.1 GHz. The absorption bandwidth with RL less than 10 dB is 4.2 GHz (12.2–16.4) with coating thickness of only 1.5 mm; hence, Co-doping can enhance the microwave absorption performance of SnO2@MWCNTs. The excellent microwave absorption performance may be attributed to interfacial polarization, conductivity loss, and changes in electromagnetic parameters and lattice constant caused by Co-doping. Consequently, Co-doped SnO2@MWCNTs can be considered as efficient microwave absorbers in high-frequency range.

Facial Synthesis of Zn-Doped Fe3O4 with Enhanced Electromagnetic Wave Absorption Performance in S and C Bands

Zhenfeng Liu,Honglong Xing,Lei Wang,Dexin Tan,Ying Gan,Xiaoli Ji,Guocai Xu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.8

In this study, Zn-doped Fe3O4 nanoparticles were successfully synthesized by a facile solvothermal method in the presence of sodium dodecyl sulfate (SDS). The morphology, magnetic properties and electromagnetic wave absorbing properties of these materials were characterized. Results showed that Zn2+ played a significant role in the formation of Zn-doped Fe3O4. With the protection of SDS, highly dispersed Fe3O4 nanoparticles were obtained. The nanoparticle size decreased after Zn2+ doping, and the dispersity deteriorated with increasing Zn2+ doping concentration. Zndoped Fe3O4 exhibited excellent electromagnetic wave absorbing property, which resulted in magnetic loss and dielectric loss at an appropriate doping concentration. The minimum reflection loss (RL) was approximately -27.2 dB at 16.9 GHz. As the coating layer thickness increased to 4.0 mm, the bandwidth was approximately 5.0 GHz corresponding to RL below -10 dB, which nearly covered the entire S band (2–4 GHz) and C band (4–8 GHz). The peak frequency of RL and the number of peaks matched the quarter-wave thickness criteria. It was believed that the Zndoped Fe3O4 could be a potential electromagnetic wave absorbing material in S and C bands.