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Yanxia Chen,Xuedong Liu,Xiaoguang Yang,Yuhui Liu,Xiaomeng Pi,Qingzhen Liu,Dong Zheng 한국유전학회 2015 Genes & Genomics Vol.37 No.5
Deer antlers are the only mammalian appendages subject to an annual cycle of epimorphic regeneration. Within the rapid-growth stage, they display the fastest cartilage development in the animal kingdom. To identify microRNA (miRNA) profiling in red deer (Cervus elaphus) antler cartilage, we applied deep sequencing technology to a small RNA library constructed from pooled cartilage (three antlers from three individuals). We generated 9,520,645 mappable reads with a size distribution of between 15 and 30 nucleotides (miRNAs of 18 nucleotides were the most abundant group: 31 %). Bioinformatics data mining revealed 399 miRNAs in antler cartilage, of which 345 were highly conserved and expressed in 25 other mammals, including the cattle (Bos taurus) and in humans (Homo sapiens). The remaining 54 miRNAs we identified were novel and likely to be antler-cartilage specific, but were expressed at low levels. The identification of these known and newly identified miRNAs in antler cartilage significantly enhances our understanding of the miRNA profiling of regenerating antler cartilage. Further studies are necessary to better understand miRNAs-regulated antlerogenesis.
Cunshuan Xu,Xiaoguang Chen,Cuifang Chang,Gaiping Wang,Wenbo Wang,Lianxing Zhang,Qiushi Zhu,Lei Wang,Fuchun Zhang 한국유전학회 2012 Genes & Genomics Vol.34 No.3
It has been widely accepted that hepatocytes are critical for liver regeneration (LR), but very little is known about the role of biliary epithelial cells (BECs) in this event, so this study aims to manifest the relevance of BECs with LR. High purity population of BECs was obtained using Percoll gradient centrifugation combined with immunomagnetic-bead separation technique. Transcriptional profiles of BECs from rat regenerating liver after 2/3 hepatectomy were monitored with rat genome 230 2.0 array. Microarray analysis results were evaluated by RT-PCR assays. Of all the genes on the array, 1262 known genes and 1026 unknown genes were related to LR. 79 of 1262 known genes showed a ≥ 20-fold change in expression level, mainly participating in primary metabolism and inflammatory response. In contrast to the regenerating liver,BEC division did not occur at proliferative phase of LR; alterations in nucleic acid, lipid and protein metabolism were significantly different from each other or within the same substance metabolism at different phases; the active signaling pathways in priming phase were mediated mainly by G protein-coupled receptor, small GTPase and Wnt receptor. Transport-related genes showed up-regulated expression mainly in priming and proliferative phases, possibly linked to cell membrane formation and transport function recovery of BECs in the late phase. In brief, comparative analysis of biological activities of BECs and the regenerating liver reveals that bio-logical activities at the cellular level are not always consistent with those at tissue level, suggesting the necessity of cell level investigation on liver regeneration. Finally, expression of BEC markers in hepatocytes may suggest the potential of hepatocytes to transdifferentiate into BEC.
Analysis of Gene Expression Profiles of Liver Stellate Cells During Liver Regeneration in Rats
Xu Cunshuan,Chen Xiaoguang,Chang Cuifang,Wang Gaiping,Wang Wenbo,Zhang Lianxing,Zhu Qiushi,Wang Lei,Zhang Fuchun 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.1
This study performed a large-scale, high-throughput analy-sis of transcriptional profiling of liver stellate cells (LSCs) at the cellular level to investigate changes in the biological activity of LSCs during rat liver regeneration (LR) and the relation of these changes to LR. First, a rat liver regeneration model was established by partial hepatectomy (PH). Stellate cells were isolated in high purity and yield from the regenerating rat liver by Percoll density gradient centrifugation and immunomagnetic bead sorting. The changes in gene expression of LSCs after PH were examined using a rat genome 230 2.0 array composed of 24622 genes. The results indicated that 10241 of the 24622 genes investigated on the array were differentially expressed in LSCs. Of the 10241 genes, 1563 known genes were related to LR, which were grouped into three major gene expression clusters according to three-fold cut-off threshold: the up-regulated gene cluster, the down-regulated gene cluster, and the cluster composed of genes showing complex changes in expression. Additionally, the genes were grouped into those involved in transcription regulation, signal transduction, transport, cellular metabolism, in-flammation and immunity by functional analysis. When gene expression profiles were combined with the results of gene functional analysis, most of the genes involved in cytokine secretion and retinol metabolism in LSCs were significantly enriched in the cluster characterized by decreased expression, whereas genes involved in lipid metabolism were mostly enriched in the cluster showing increased expression. Based on further analysis of genes expressed in a phase-dependent manner during LR, it was suggested that lipid metabolism in LSCs was enhanced in the whole regeneration process, and that immune response and cytokine secretion were impaired during all three regenerative phases.
Zhiyi Tu,Liangliang Chen,Qiong Li,Xiaoguang Jin,Jiaju Wu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12
This paper proposes a new design method for sleeve thickness and interference based on a multi-dimensional visualization algorithm that overcomes difficulties in solving. The contact pressure between the rotor core and the permanent magnet, the maximum equivalent Mises stress of the permanent magnet, and the maximum equivalent Mises stress of the sleeve are selected as the optimization objectives. The stress limit strength of the permanent magnet and sleeve are taken as constraints, and the sleeve thickness and interference are selected as decision variables. Then the multi-dimensional visualization algorithm is used to find the optimal region of sleeve thickness and interference. Finally, the design solution is validated by the finite element method. The results show that the design method proposed in this paper can obtain the optimal region of sleeve thickness and interference with the characteristics of a simple solution process and intuitive results.
BRG1 Promotes chromatin remodeling around DNA damage sites
Wenjing Qi,Hongyu Chen,Chengwen Lu,Qingpan Bu,Xiaoguang Wang,Liping Han 한국통합생물학회 2018 Animal cells and systems Vol.22 No.6
Chromatin remodeling complexes play important roles in various DNA metabolism processes, including DNA damage repair. BRG1 is the core subunit of the SWI/SNF complex, which plays critical roles in cell cycle regulation, cell development, cell differentiation, and tumorigenesis. In the present study, we report that BRG1 depletion increased the percentage of apoptotic cells in etoposide-treated cells. Moreover, western blotting and immunofluorescence data showed that BRG1 depletion decreased H2AX phosphorylation and caused defective phosphorylated histone H2AX (γH2AX) clearance. Furthermore, we found that in both SW13 and U2OS cells, BRG1 expression could increase the sensitivity of genomic DNA to micrococcal nuclease (MNase) and facilitate chromatin relaxation around DNA damage sites. Thus, the results provide evidence that BRG1 plays an important role in early DNA damage repair by remodeling the chromatin structure near DNA damage sites.
Cerebrospinal Fluid Leakage after Surgeries on the Thoracic Spine: A Review of 362 Cases
Panpan Hu,Miao Yu,Xiaoguang Liu,Zhongjun Liu,Liang Jiang, Feng Wei,Zhongqiang Chen 대한척추외과학회 2016 Asian Spine Journal Vol.10 No.3
Study Design: A retrospective clinical review. Purpose: To describe the incidence of cerebrospinal fluid leakage (CSFL) after thoracic decompression and examine the CSFL predisposing clinical factors. Overview of Literature: CSFL is a common complication following thoracic decompression but has not been sufficiently addressed in former studies. Methods: A cohort of 362 cases of thoracic decompression from February of 2005 to June of 2013 was examined. The case medical records were reviewed and the occurrence of CSFL and the related clinical parameters were noted. The incidence of CSFL for the entire cohort and each surgical approach were described. Besides, the relationship between CSFL and other clinical parameters were assessed, of which odds ratio values of all CSFL-associated parameters were calculated using multivariate logistic regression analysis. Results: The incidence of CSFL for the entire cohort was 32.3%. Different surgical approaches had different incidences of CSFL, and circumferential decompression had the highest incidence. Though many different clinical parameters were related to the occurrences of CSFL, being older than 52 years, having ossification of the posterior longitudinal ligament or having longer operative segments than 3 vertebrae were significant risk factors for CSFL (p <0.05). Besides, surgeries on the mid-thoracic spine had an increased risk of CSFL (p <0.05). Conclusions: From our analysis, CSFL was a common complication after thoracic decompression with the incidence of up to 32.3%. This study identified the predisposing clinical factors, and spinal surgeons should be aware of these risk factors to reduce its incidence.
( Zixing Dong ),( Xiaoling Chen ),( Ke Cai ),( Zhixin Chen ),( Hongbin Wang ),( Peng Jin ),( Xiaoguang Liu ),( Kugenthiren Permaul ),( Suren Singh ),( Zhengxiang Wang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.3
Owing to its high protein secretion capacity, simple nutritional requirements, and GRAS (generally regarded as safe) status, Bacillus licheniformis is widely used as a host for the industrial production of enzymes, antibiotics, and peptides. However, as compared with its close relative Bacillus subtilis, little is known about the physiology and stress responses of B. licheniformis. To explore its temperature-stress metabolome, B. licheniformis strains ATCC 14580 and B186, with respective optimal growth temperatures of 42℃ and 50℃, were cultured at 42℃, 50℃, and 60℃ and their corresponding metabolic profiles were determined by gas chromatography/mass spectrometry and multivariate statistical analyses. It was found that with increased growth temperatures, the two B. licheniformis strains displayed elevated cellular levels of proline, glutamate, lysine, pentadecanoic acid, hexadecanoic acid, heptadecanoic acid, and octadecanoic acid, and decreased levels of glutamine and octadecenoic acid. Regulation of amino acid and fatty acid metabolism is likely to be associated with the evolution of protective biochemical mechanisms of B. licheniformis. Our results will help to optimize the industrial use of B. licheniformis and other important Bacillus species.