http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Deyuan Tang,Jian Liu,Chunyan Li,Hua Zhang,Ping Ma,Xianfeng Luo,Zhiyong Zeng,Nining Hong,Xia Liu,Bin Wang,Feng Wang,Zhenlei Gan,Fei Hao 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.1
The purpose of this study was to investigate the effects ofporcine interleukin (IL)-2 and IL-4 genes on enhancing theimmunogenicity of a porcine reproductive and respiratorysyndrome virus ORF5 DNA vaccine in piglets. Eukaryoticexpression plasmids pcDNA-ORF5, pcDNA-IL-2, andpcDNA-IL-4 were constructed and then expressed in Marc-145cells. The effects of these genes were detected using an indirectimmunofluorescent assay and reverse transcriptionpolymerase chain reaction (RT-PCR). Characteristicfluorescence was observed at different times after pcDNAORF5was expressed in the Marc-145 cells, and PCR productscorresponding to ORF5, IL-2, and IL-4 genes were detected at48 h. Based on these data, healthy piglets were injectedintramuscularly with different combinations of the purifiedplasmids: pcDNA-ORF5 alone, pcDNA-ORF5 + pcDNA-IL-2,pcDNA-ORF5 + pcDNA-IL-4, and pcDNA-ORF5 + pcDNAIL-4 + pcDNA-IL-2. The ensuing humoral immune responses,percentages of CD4+ and CD8+ T lymphocytes, proliferationindices, and interferon-γ expression were analyzed. Resultsrevealed that the piglets co-immunized with pcDNA-ORF5 +pcDNA-IL-4 + pcDNA-IL-2 plasmids developed significantlyhigher antibody titers and neutralizing antibody levels, hadsignificantly increased levels of specific T lymphocyteproliferation, elevated percentages of CD4+ and CD8+ Tlymphocytes, and significantly higher IFN-γ production thanthe other inoculated pigs (p < 0.05).