Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

정상면역을 가진 성인에서 발생한 식도 방선균증

김현수 ( Hyun Soo Kim ),천종운 ( Jong Woon Cheon ),김민수 ( Min Su Kim ),정창길 ( Chang Kil Jung ),김경록 ( Kyung Rok Kim ),최재원 ( Jae Won Choi ),강동우 ( Dong Woo Kang ),김선영 ( Sun Young Kim ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Hyun Soo Kim, Jong Woon Cheon, Min Su Kim, Chang Kil Jung, Kyung Rok Kim, Jae Won Choi, Dong Woo Kang and Sun Young Kim1 Departments of Internal Medicine and Pathology1, DongKang Medical Center, Ulsan, Korea Actinomycosis is a chronic suppurative disease and caused by Actinomycosis species, principally Actinomyces israelii, which are part of the normal inhabitant on the mucous membrane of the oropharynx, gastrointestinal tract, and urogenital tract. It usually affects cervicofacial, thoracic and abdominal tissue. Cervicofacial type has the highest percentage of occurrence with 50%. Actinomycosis frequently occurs following dental extraction, jaw surgery, chronic infection or poor oral hygiene. It may also be considered as an opportunistic infection in immunocompromised patients such as malignancy, human immunodeficiency virus infection, diabetes mellitus, steroid usage or alcoholism. But, actinomycosis rarely occurs in adults with normal immunity and rare in the esophagus. We report an unusual case of esophageal actinomycosis which was developed in a patient with normal immunity and improved by therapy with intravenous penicillin G followed oral amoxicillin, and we also reviewed the associated literature.

홍당무 모자이크바이라스에 관한 연구

김은수(Woon Soo Kim),최예진(Yae Chin Choi) 한국식물학회 1963 Journal of Plant Biology Vol.6 No.2

KIM, Woon Soo* & CHOI, Yae Chin**(University of Ill., U.S.A.* & Chunchon Agricultural Coll.**) Studies on the viruses of radish mosaic. Kor. Jour. Bot. VI (2):9-21, 1963. A mosaic diseased radish collected from the suburb of Seoul, in November, 1961 was used for studing the host range, physical properties, purification, insect transmission, and electron microscopy. A Japanese strain of raddish mosraic (RPV) was also used with Korean strain of radish mosaic (KRMV) for a comparative study. The two viruses, KRMA and RPV, were identified by the difference in host range, insect transmission and electron microscopy. The KRMA was severely infective on tobacco and Nicotiana glutinosa, while on Gomphrena globosa was immune to the virus. RPV produces necrotic local lesions on Comphrena globosa but did not infect tobacco and N. glutinosa. Among varieties of radish, Seoul, Akamaroo, Akanagea, Koong-Joong showed more severe symptoms than Simoo, Minong, Paek-soo, which appeared to be fainly resistant. In a number of tests, it was found that the virus KRMA retained its infectivity until to a dilution of 1:2,000, heating at 58˚C for 10 minutes, and aging in vitro for 7 days at room temperature. The RPV was not inactivated until it was diluted to 1:2,000, heated to 56。C, and aged for 6days. The KRMV was readily transmitted by the aphid (Myzus persicae Sulz). The virus RPV was not transmitted by the aphid in a number of tests. Partialy purified viruses using ammonium acetate buffer, salting-out by ammonium sulfate and centrifugation of high and low speed were highly infective. Electron micrographs showed that the KRMV paticles are of spherical particles whereas the RPV particles are rod-shaped.

하악골 절제술후 재이식된 돌결자가골 재이식 : 치험 3례

김 식,김수관,김운규,김수민,류종희,이준길,조경안,김수흥,정태영,여환호 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.2

We described three cases of immediate reimplantation of a frozen-thawed autogenous mandible composed of a mixture of iliac bone, marrow, and particulate hydroxyapatite in tumors of the mandible. Acceptable outcomes were obtained in three patients who underwent immediate autogenous mandibular graft reconstruction. The conditions leading to successful outcome of the procedure are also discussed. Reimplantation of frozen autogenous lesioned mandible was performed in three patients with mandibular tumors. Two reimplanted grafts survived without complications following surgery. One case had postoperative infection that resolved with appropriate antibiotic treatment. There were no recurrences of the primary lesions. Satisfactory facial contour after surgery was achieved. These results are most promising, and we believe that, with further refinement, this technique will offer a new and acceptable modality for facial reconstruction in patients with cancer.

Microstructure‑Strengthening Interrelationship of an Ultrasonically Treated Hypereutectic Al–Si (A390) Alloy

Soo‑Bae Kim,Young‑Hee Cho,Jae‑Gil Jung,Woon‑Ha Yoon,Young‑Kook Lee,Jung‑Moo Lee 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6

Ultrasonic melt treatment (UST) was applied to an A390 hypereutectic Al–Si alloy in a temperature range of 750–800 °Cand its influence on the solidification structure and the consequent increase in strength was investigated. UST at such a hightemperature, which is about 100 °C above the liquidus temperature, had little effect on the grain refinement but enhanced thehomogeneity of the microstructure with the uniform distribution of constituent phases (e.g. primary Si, α-Al and intermetallics)significantly refined. With the microstructural homogeneity, quantitative analysis confirmed that UST was found tosuppress the formation of Cu-bearing phases, i.e., Q-Al5Cu2Mg8Si6, Al2Cuphases that form in the final stage of solidificationwhile notably increasing the average Cu contents in the matrix from 1.29 to 2.06 wt%. A tensile test exhibits an increasein the yield strength of the as-cast alloy from 185 to 208 MPa, which is mainly associated with the solute increment withinthe matrix. The important role of UST in the microstructure evolution during solidification is discussed and the mechanismcovering the microstructure-strengthening interrelationship of the ultrasonically treated A390 alloy is proposed.

Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells

Lee, Dahae,Lee, Dong-Soo,Jung, Kiwon,Hwang, Gwi Seo,Lee, Hye Lim,Yamabe, Noriko,Lee, Hae-Jeong,Eom, Dae-Woon,Kim, Ki Hyun,Kang, Ki Sung The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.1

Background: The aim of the present study was to evaluate the potential protective effects of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from Panax ginseng against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells. Methods: LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay. Results: Reduction in cell viability by $60{\mu}M$ FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 ($10{\mu}M$ and $50{\mu}M$). Conclusion: The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.

서울大學校學生의 赤血球沈降速度에 關한 硏究 : 結核集團檢診의 疫學的方法을 爲하야

金仁達,權彛赫,車喆煥,柳運相,尹德老,林道洙 서울대학교 1957 서울대학교 論文集 Vol.5 No.-

The Eryshrocyte Sedimentation Rate on the students of Seoul National Uuiversity was discussed corelating with X-ray findings for tuberculosis mass suruey. 1) Among the students of Seoul National University, 4727 were examined by miniature radiogram and the morbidity of active tuberculosis was shown 1.7 percent 616 of students were examined by both miniature radiogram and blood sedimentation reaction and the morbidity was 2.9 per cent. 2) The screening procedure by blood sedimentation reaction in addition to miniature radiogram could prevent missing around one fourth of active cases This means that E.S.R. has a great significance on the screening procedure for tuberculosis case findings. 3) Abnormal E.S.R. was shown on 75,5 percent of active cases. Persons whose E.S.R. are beyond 3mm for 30minutes and 10mm for 60minutes are suspected tuberculosis and should be required further examination. 4) The distribution rate of E.S.R. of between 0∼6.5mm was shown on 80percent of these students who were examined and that of between 0∼10mm was shown on 88.7 percent of students. The mean was 4.8mm. Hereby it is recommended That E.S.R. must be employed as one part of the useful methods on the mass screening survey for tuberculosis. furthermore it should be recalled that most of the Korean youths show tuberculin test positive. This fact requires such procedure as blood sedimentation reaction in addition to X-ray photography urgently on the epidemiological tuberculosis survey.

교사들의 직무스트레스, 조직풍토, 여가활동 참여가 직무태도에 미치는 영향

김재현,이수운 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

This study aims to analyze the relationship between teachers' job stress, job attitude and climate of their organizations and provide basic materials needed for developing program improving their job attitudes through leisure activities. To achieve purposes of the research, it selected 160 elementary school teachers in Gwangju and Jeonnam regions as of January of 2004 by simple random sampling method. Data used for actual analysis were analyzed with one-way ANOVA, t-test and gradual regression analysis using statistical program SPSS 10.1. It obtained the following conclusions through these research methods and data analysis. First, it was found that there was a difference in supportive behavior which was sub-factor of climate of organization by sex and in supportive and directive behaviors by age and working careers and there was a significant difference in supportive, directive and preventive behaviors by annual salary and marriage. Second, the more frequency of participation in leisure activity, the less job stress. In less than one year for leisure activity, involvement in organization and supportive behaviors were strongest. Third, as a result of regression analysis with single variable as job attitude, variables explaining this factor significantly were class activity which was sub-factor of climate of organization and friendly behavior among 21 independent variables. When job attitudes were divided into job satisfaction, involvement in organization and tendency to transfer job, sub-factors predicting job satisfaction included friendly, indifferent and supportive behaviors, annual salary and class activities and variables predicting involvement in organization included academic and friendly behaviors ad age. In addition, variables predicting tendency to transfer job included class activity, indifference and participation in leisure activities.

수리·통계모형에 의한 설계 갈수량 추정법 개발

김운중,김영균,문수회 朝鮮大學校 建設技術硏究所 2005 建設技術硏究 Vol.25 No.1

The method of a frequency analysis which takes advantage of annual low flow data is extensively used in our country when the design low flow is estimated. It is necessary to secure the measurement record of a long-term in this method and therefore it is not proper to apply this method for cases of measurement record of a short-term or an ungaged outlet. In work-site, generally, the design low flow of unmeasured station is determined by the secondhand method which utilizes the area ratio between the gaging station and the unmeasured station. However, this method using the area ratio is unreal and unreliable because the low flow that is different from the flood flow is affected by the topographical, the geological and the basin characteristics. A new proposal that estimates the design low flow by using the hydraulic-statistical model is made in this paper. A method the return period can be converted into the recession time is derived under an assumption that the dry spell is similar to the low-flow runoffs event. To induce the method(recession time model), two kinds of models are employed; One is the type I extremal value distribution as the dry spell model, and the other is the exponential linear recession equation as the low-flow runoffs model. The climate conditions, the watershed and the runoff characteristics are adopted as the parameters of the recession time model. Meanwhile, a recession model that can estimate the design low flow in the gaging station or the unmeasured station is made by using the results of the recession time model. The initial discharge and the recession constant are used as the parameter of the recession model. The present model derived from this study is applied into the outlet basin of Yongdam gaging station, and the estimated design low flow for the return period of 10 year is obtained as 2.14m3/sec which corresponds to 97% of standard low flow (2.22m3/sec). Resultantlv, it is concluded that the present model proposed in this paper can be effectively used as the alternative for estimation of the design low flow.

치아종 제거후 시행한 자가치아이식술

김종운,김수관,김수흥,정태영 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

We report a case in which a right mandibular incisor was impacted beneath an odontoma in the anterior mandible. An odontoma at the symphyseal region was removed under general anesthesia, and bone was grafted. Tooth #41, which was beneath the lesion, was extracted and transplanted to its original site. It was splinted with resin for 2 weeks and was checked at intervals during that time. After 4 months, it was filled endodontically with calcium hydroxide.