비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

구강에 발생한 편평상피암종의 MMP-2, MMP-9, TIMP-1, TIMP-2 발현과 EGFR 및 E-cadherin 발현의 상호연관성

조성재,권창석,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Matrix metalloproteinases(MMPs) are involved in the degradation of extracellular matrix, which is related to infiltrative growth and metastasis of tumor. Positive correlation between MMPs expression and clinico-pathologic parameters were reported, and MMPs expressions were regulated by tissue inhibitor of metalloproteinases(TIMPs) or cell adhesion molecules such as E-cadherin and epidermal growth factor receptor EGFR). The aim of this study is to evaluate the relationship between MMP-2, MMP-9 expressions and clinico-pathologic factors such as clinical stage. recurrence. histologic grade and invasion pattern, and between MMP-2 and MMP-9 Expressions and TIMP-1, TIMP-2, and E-cadherin expressions. Methods and Material : The materials were 55 cases of squamous cell carcinoma of the oral cavity, and immunohisdtochemical studies for MMP-2, MMP-9, TIMP-1, TIMP-2, E-cadherin and EGFR were performed. Clinico-pathologic factors such as clinical stage, recurrence, histologic grade and invasion pattern were recorded through microscopic examination and clinical record review. Statistical analyses between MMP-2, MMP-9 expressions and clinico-pathologic factors, TIMP-1, TIMP-2 expressions and EGFR and E-cadherin expressions were done. Results : 1. MMP-2 and MMP-9 expressions were noted in 30(54.5%) and 22(40.0%) of 55 cases, respectively. TIMP-1 and TIMP-2 expressions were observed in 21(38.2%) and 33(60.0%), and E-cadherin and EGFR expressions were found in 35(63.6%) and 26(47.3%) of 55 cases, respectively. 2. MMP-2 expression rate was slightly higher in cases without recurrence, and TIMP-2 expression rate was slightly higher in cases showing more infiltrative growth pattern. However, there were no significant differences of MMP-2, MMP-9, TIMP-1, and TIMP-2 expression rates according to other clinico-pathologic parameters were noted. 3. The expression rate of EGFR was higher in cases with well differentiation (p=0.0047), but no positive relationship between the expression rate of E-cadherin and histologic grade was found. There were no significant differences of the expression rates of E-cadherin and EGFR according to other clinico-pathologic parameters. 4. Cases with positive reaction for MMP-9 showed an increasing tendency of negative reaction for TIMP-1. However, signigicant relationships between MMP-2, MMP-9 expressions and TIMP-1, TIMP-2 expressions were not noted. 5. The expression rate of MMP-2 was higher in cases with positive reaction for Ecadherin and EGFR with no statistical significance. The expression rate of MMP-9 was significantly higher in cases with positive reaction for E-cadherin(p=0.0221), but there was no significant relationship between MMP-9 and EGFR expression. Conclusion : These results suggest that MMP-2, MMP-9, TIMP-1 and TIMP-2 expressions are involved in the development of oral squamos cell carcinomas, but MMP-2, MMP-9, TIMP-1, and TIMP-2 expressions might not seem to be a sueful prognostic factors because there were no significant relationship between clinicopathologic parameters, EGFR expression showed positive correlation with low histologic grace, so EGFR expression could be regarded as a good prognostic factor. In the progression of squamous cell carcinoma of oral cavity, MMP-9 and E-cadherin might be involved with positive correlation, but there were no significant correlations between MMP-2, MMP-9 and TIMP-1, TIMP-2 and EGFR expressions rate. Further study for other factors such as cytokines or growth factors except TIMP-1, TIMP-2 and EGFR will be needed.

최근 5년간 약진으로 입원한 환자의 고찰 - 임상양상 및 검사소견(1986∼1990) -

고우석,조광현,윤재일 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

성인 급성 골수성 백혈병에서 6-Thioguanine, Cytarabine, Doxorubicin(TAD) 3제 복합화학요법의 효과 : 최종보고

최환석,윤휘중,우인숙,박지훈,김정희,김시영,조경삼 大韓血液學會 1995 大韓血液學會誌 Vol.30 No.2

태권도 우수선수들의 정신력 특성

최상진,조옥성,백우석,이갑철,고봉수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to investigate the mental toughness of Taekwondo players and to suggest way of performance enhancement using Loehr's Psychological Performance Inventory. Subjects were 188 Taekwondo elite players. Each factor's mean and standard deviation of Mental Toughness were calculated, and ANOVA were used for statistical analyses of the difference of mean. The results are as follow;: 1. On the level of self-confidence, it shows that the man players is higher than the woman players (school career F=5.846, P<.05: sex distinction F=8.876, P<.01). 2. On the level of attention control, it shows that the woman players is higher than the man players (school career F=4.483, P<.05: sex distinction F=9.814, P<.01). 3. On the level of visual and imagery control, it shows that the man players is higher than the woman players (school career F=7.790, P<.01: sex distinction F=5.000. P<.05). 4. On the level of arousal control, motivation, positive energy and attitude control, it was not significant difference according to the sexual and school career.

환자만족도 조사설문지의 설문문항 배열에 따른 신뢰도 분석

최귀선,조우현,홍재석,이선희,강명근 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.2

Objectives : The purpose of this study was to evaluate the reliability of questionnaire according to item arrangement on patient satisfaction questionnaire. Methods : We developed the two types of questionnaire with different item arrangement. In the first type (A), questions were arranged according to medical service dimensions. Questions in the second type (B) were arranged according to medical process. Both questionnaires were composed of six dimensions: physical environments, process, competence, courtesy, information giving, understanding patients. Measurements were performed on a 5-score Likert scale. In an outpatients and inpatient survey, total 777 patients answered the type A (outpatients: 257, inpatients: 128) or Type B (outpatients: 257, inpatients: 135). In order to compare the internal consistency of two types. Cronbach's α were calculated. Multiple regression analysis was also performed to know which type of questionnaire explain more of the overall satisfaction. Results : In outpatient survey, type A questionnaire showed higher internal consistency than B except physical environments dimension. Also in inpatient survey, type A had higer internal consistency than B in four dimensions(process, competence, courtesy, understanding patients). In the results of multiple regression analysis, type A questionnaire (R² =0.53) explained more of the variation in overall satisfaction then B questionnaire (R2=0.43) in outpatient survey. In inpatient survey, type B questionnaire (R²=0.40) explained, more of the variation in overall satisfaction than type A questionnaire (R² =0.33).But the.difference of R was not significant in inpatient survey. Conclusion : The results of this study support that type A questionnaire has higer reliability in assessment of consumer satisfaction than type B.

국내에서 분리된 Esherichia Coli 와 Klebsiella pneumoniae의 CTX-M형 Extended-Spectrum β-Lactamase 생성 현황

배일권,우건조,정석훈,박광옥,조병규,김둘만,권수봉,김현주,강현경 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

탄소성 변형을 받는 냉간 압연강의 X선 잔류응력 특성에 관한 연구

홍순혁,이동우,양광호,조석수,주원식 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.1

In textured material, diffraction angle 2θ usually shows a nonlinear relation against sin^2Ψ due to elastic anisotropy of crystals. SPHD and SPCD steel is cold-rolled carbon steel for automobile. The characteristics X-ray for stress measurement is Cr K_α and Mo K_α1characteristic X-ray. The sin^2Ψ-2θ diagram under elastic strain seems to have a linear behavior using regression line of data but has a nonlinear behavior in distribution of data by Cr K_αcharacteristic X-ray. As the plastic strain of specimen increases, the nonlinearity of 2θ with respect to sin^2Ψ increases remarkably. On the other hand, the diffraction angle 2 θ by Mo K_α1 characteristic X-ray shows a good linearity on sin^2Ψ-2θ diagram under plastic strain as well as elastic strain. Therefore, This paper presents the measurement of residual stress in cold-rolled carbon steel for automobile using penetration depth of Mo K_α1 charateristic X-ray and multiplicity factor of crystal diffraction plane.

신경회로망을 이용한 원공 결함 패턴 인식에 관한 연구

홍순혁,이동우,허건,조석수,주원식 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.1

Ultrasonic inspection of defects has been focused on the existence of defect in structural material and need has much time and expenses in inspecting all the coordinates (x, y) on material surface. Neural networks is information processing system modeling human brain and can have an application to coordinates (x, y) of defects by multi-point inspection method. Ultrasonic inspection modeling is optimized by neural networks which makes defect echo, back-wall echo and ultrasonic transducer units of input layer and absolute and relative coordinates of defects and defect pattern units of output layer. This method can predict coordinates (x, y) of defects within engineering estimated mean error Ψ.