http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Woo, Seonock,Won, Hyokyoung,Lee, Aekyung,Yum, Seungshic 대한독성유전단백체학회 2012 Molecular & cellular toxicology Vol.10 No.2
Transcriptional changes in the expression of stress-related genes (catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, metallothionein, and ubiquitin) in intestinal, liver and muscle tissues of Javanese medaka (Oryzias javanicus) exposed to $17{\beta}$-estradiol (E2) were investigated using real-time quantitative PCR. The lipid peroxidation levels and superoxide dismutase activity in liver tissues was also examined at various exposure concentrations. In fish exposed to 10, 100 and 1000 ${\mu}g/L$ E2 the level of catalase mRNA increased significantly in intestinal and muscle tissues, while expression of the glutathione peroxidase gene showed the opposite trend in liver and muscle tissues. Expression of the glutathione S-transferase gene increased in a dose-dependent manner in liver and muscle tissues of fish exposed to E2, relative to the non-exposed control group. The level of glutathione reductase mRNA increased markedly in the liver tissues of all fish exposed to 100 and 1000 ${\mu}g/L$ E2, but decreased in intestinal tissues. The metallothionein gene was strongly downregulated in intestinal and liver tissues of fish exposed to E2, but slightly upregulated in muscle tissues. In all fish exposed to 100 and 1000 ${\mu}g/L$ E2 expression of the ubiquitin gene increased markedly in liver and muscle tissue, but not in intestinal tissues. Lipid peroxidation and superoxide dismutase activities increased significantly in all $17{\beta}$-estradiol treatment groups, and were correlated to the exposure concentration. Thus, E2 exposure differentially affected the transcription of a range of stress-related genes in various tissues of Javanese medaka, suggesting that analysis of transcriptional changes in these genes could be used as a rapid assay of the effects of E2 exposure.
Expression profiling of liver in Java medaka fish exposed to 17β-estradiol
Seonock Woo,Hye-Young Jeon,Taek Kyun Lee,Seong-Ryul Kim,이승훈,염승식 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.3
Java medaka (Oryzias javanicus) cDNA array was constructed and the microarray platform was used to compare the hepatic expression profiles of Java medaka fish exposed to 17β-estradiol with those of unexposed controls. Data analysis demonstrated that the expression profiles were strongly affected by 17β-estradiol exposure, with 655 genes up- or downregulated after 24 h, and 633 genes after 48 h. The differentially expressed genes were analyzed to determine the effects of 17β-estradiol exposure on the liver tissue and were classified into five functional categories: information storage and processing, cellular processes and signaling, metabolism, general function prediction only, and function unknown. Genes whose expression was upregulated more than 10-fold were predominantly associated with energy production/conversion and reproduction,and 30% of the genes whose expression was downregulated more than 10-fold were associated with carbohydrate transport and metabolism. The observed differences in the expression profiles of 7genes (encoding apolipoprotein B, cytochrome P4501A, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase 1b, vitellogenin, selenoprotein M, and transferrin) were confirmed by quantitative RT-PCR,and their transcriptional changes in the livers of Java medaka induced by exposure to 10, 100, and 1,000μg/L 17β-estradiol were investigated. These results should allow the development of biomarkers for the identification of 17β-estradiol contamination in the environment and provide molecular biological information on the effects of endocrine-disrupting chemical exposure on marine animals.
Woo, Seonock,Yoon, Moongeun,Suh, Dong-Sang,Song, Jun-Im,Kim, Jeong Ha,Yum, Seungshic 한국유전학회 2004 Genes & Genomics Vol.26 No.4
The complete cDNA of eukaryotic translation elongation factor 1A (eEF1A) from the soft coral Scleronephthya gracillimum (Ku¨kenthal) (Alcyonacea, Octocorallia, Anthozoa, Cnidaria) was cloned by RT-PCR and the nucleotide sequences were determined. The information on primary structure of the eEF1A gene and protein may be useful for the gene expression study as well as for the phylogenetic study. The full-length cDNA of S. gracillimum eEF1A comprised 1612 bp, not including the poly (A)^+ stretch, and contained a complete open reading frame encoding 461 amino acids. The deduced amino acid sequence showed 85%identity to placozoan, 86% to human and 77% to hydra. Considering that functional domains are highly conserved throughout the evolutionary history, the present study suggests that the amino acid sequence of eEF1A alone may not resolve the phylogenetic relationship of lower metazoans. A possible solution to overcome the long branch problems that appear in cnidiarian phylogeny is discussed.
Transcriptome dynamics in benzo[a]pyrene exposed Hydra
Lee Nayun,Woo Seonock,Lee Nayoung,Jo Yejin,Yamindago Ade,Yum Seungshic 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.3
Backgrounds Benzo[a]pyrene (BaP) is a well-known ecotoxicant that induces a wide spectrum of toxic effects in organisms, including carcinogenicity, teratogenicity, genotoxicity, and immunotoxicity. Thus, re-evaluation of its acute toxic effects in gene expression at sublethal concentration in experimental animal is essential to understand and/or predict metabolic and physiological changes in an organism after exposure. Objectives To understand the changes in acute toxicity by exposure time, differential gene expression profiling of Hydra magnipapillata was performed by DNA microarray after exposure to BaP. Results The median lethal concentrations of the animals (LC 50 ) were determined to be 78.5, 53.6, and 28.9 mg/L after exposure to BaP for 24, 48, and 72 h, respectively. Morphological responses of hydra polyps to 50 mg/L of BaP by exposure time were observed. The gene expression levels of molecular chaperones, antioxidative enzymes were altered at the early phase of the exposure. Transcription of the genes related to development and differentiation; apoptosis and necrosis were affected by the 4 h BaP exposure group. After 12 h exposure, developmental processes, immune responses, and ion transport in hydra polyp seemed to be affected, since the transcriptions of the genes that related to those biological processes were induced or repressed by BaP exposure. Neurotransmission might be suppressed, but tumorigenesis and carcinogenesis, the DNA repair process might be induced in the 24 h BaP-exposed hydra group. Finally, tumorigenesis and carcinogenesis, and the DNA repair process seemed to be induced after 48 h exposure. Conclusion We successfully demonstrated the dynamic response of Hydra to BaP by exposure time at transcription level. The results could extend our knowledge on acute toxic effect of BaP at sublethal conditions.
Changes in gene expression profile due to acute toxicity of toxaphene in the marine medaka
Lee, Aekyung,Woo, Seonock,Won, Hyokyoung,Lee, Gunsup,Lee, Taek-Kyun,Yum, Seungshic 대한독성유전단백체학회 2013 Molecular & cellular toxicology Vol.9 No.2
Differential gene expression profiling was performed using cDNA microarray hybridization on the hepatic tissue of the marine medaka (Oryzias javanicus) after exposure to toxaphene, which is classified as a persistent organic pollutant. Ninety-seven differentially expressed candidate genes were identified; 40 were induced and 57 were repressed (P<0.05). The genes were assembled into 18 groups based mainly on the Eukaryotic Orthologous Groups classification. These isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for identifying environmental stressors and prognosis for the biological effects of the toxicant. Some of the genes were closely related to endocrine disruption, renal and cardiovascular disease, tumorigenesis, immune responses, and detoxification. Our results will allow future studies to assess the molecular mechanisms of toxaphene toxicity and to develop a systems biology approach to environmental stress biology.
Acute toxic effects of zinc oxide nanoparticles on <i>Hydra magnipapillata</i>
Yamindago, Ade,Lee, Nayun,Woo, Seonock,Choi, Hyosun,Mun, Ji Young,Jang, Seok-Won,Yang, Sung Ik,Anton-Erxleben, Friederike,Bosch, Thomas C.G.,Yum, Seungshic Elsevier 2018 Aquatic toxicology Vol.205 No.-
<P><B>Abstract</B></P> <P>Zinc oxide nanoparticles (ZnO NPs) are increasingly used in various products as coating and additive materials for household goods, personal-care products, and drug delivery systems. Because of their broad applications, the potential risks to nontarget organisms associated with their input into aquatic environments have generated much concern. We investigated the acute toxicity, morphological responses, and potential impact on physiology and metabolism in polyps exposed to spherical ZnO NPs of either 20 nm (ZnO NP<SUB>20</SUB>) or 100 nm (ZnO NP<SUB>100</SUB>). The median lethal concentrations (LC<SUB>50</SUB>) of ZnO NP<SUB>20</SUB> were 55.3, 8.7, and 7.0 μg/mL after exposure for 48, 72, and 96 h, respectively; and those of ZnO NP<SUB>100</SUB> were 262.0, 14.9, and 9.9 μg/mL, respectively. The morphological responses of the hydra polyps to a range of ZnO NP concentrations suggest that ZnO NPs may negatively affect neurotransmission in <I>Hydra</I>. ZnO NPs may also induce abnormal regeneration in the polyps by affecting the expression of several genes related to the Wnt signaling pathway. The presence of ZnO NP<SUB>20</SUB> in the hydra tissue was confirmed with electron microscopy. A Gene Ontology analysis of the genes differentially expressed in hydra polyps after exposure to ZnO NP<SUB>20</SUB> for 12 or 24 h revealed changes in various processes, including cellular and metabolic process, stress response, developmental process, and signaling. A KEGG pathway analysis of hydra polyps after exposure of ZnO NP<SUB>20</SUB> or ZnO NP<SUB>100</SUB> for 12 or 24 h demonstrated various changes, including in the DNA replication and repair, endocytosis, lysosomes, Wnt signaling, and natural killer-cell-mediated cytotoxicity pathways, suggesting the mechanisms that maintain cellular homeostasis in response to ZnO NPs. Progesterone-mediated oocyte maturation was also affected by the ZnO NPs nanoparticles, suggesting that they are potential endocrine disruptors. This study should increase our concern regarding the dispersal of ZnO NPs in aquatic environments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Toxicity of the Zinc oxide nanoparticles (ZnO NPs) is influenced by the size of the particles and the period of exposure. </LI> <LI> ZnO NPs induce changes in the morphology and abnormal regeneration in <I>H. magnipapillata.</I> </LI> <LI> ZnO NPs penetrated and accumulated in the cells of <I>H. magnipapillata.</I> </LI> <LI> ZnO NPs induce cytotoxic and genotoxic responses. </LI> </UL> </P>