공황장애 환자에서 약물 치료와 심박동수 변이(Heart Rate Variability)의 관련성

우종민,김응석,윤혜영,최영희 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : This study was designed to evaluate relation between 6 weeks pharmacotherapy and the alteration of autonomic nervous system in patient with panic disorder. Methods : The subjects were patients (n=44) who met DSM-IV criteha for panic disorder with or without agoraphobia. They were measured Anxiety Disorder Inventory Schedule-Panic attack & agoraphobia (ADIS-P & A), Clinical Global Impression (CGI) Hamilton Rating Scale for depression (HAM-D), Panic Disorder Severity Scale (PDSS) and heart rate Vahability (HRV) before and after 6 weeks pharmacotherapy. We prescribed paroxetine primahly and benzodiazepine (clonazepam, alprazolam) in case needed. For analysis, we performed paired sample t-test, partial correlation, stepwise multiple regression. Results : After 6 weeks pharmacotherapy, a assessed clinical inventories significant improved. In HRV, patients showed stabilized sympathetic activity (LF, Normalized LF, 1og_LF), increased parasympathetic activity (Normalized HF). There were significant correlation between symptom improvement with parasympathetic components and symptom aggravation with sympathetic components. Multiple regression analysis showed that Normalized HF and TP were best explanatory variables of symptom improvement. Conclusion : This study suggests that 6 weeks pharmacotherapy is related to the alteration of autonomic nervous system and clinical improvement.

입원전담전문의 진료지원시스템의 설계 및 도입과 병원정보시스템과의 통합 방안

정윤빈,신형식,우가희,정혜경,정진경,고미영,김진수,김소원,이강영 건강보험심사평가원 2021 HIRA RESEARCH Vol.1 No.2

태권도 경기시 젖산, LDH 및 CPK의 변화에 관한 연구

김우규,윤영학,성혜련 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the changes of blood lactate, LDH and CPK in Taekwondo competition. Subjects as a T group were seven well-trained Taekwondo players. Control group was composed of seven healthy male students who taking Taekwondo club in D university. With K4b^2 telemetry, VˇO_2max was determined for each subject by administering a treadmill exercise test(5% slope, start speed: 120m/min, increased 20m/min each 2min). Data were analyzed by ANOVA with post hoc-test The 0.05 level of significance was as critical level for the study In lactate concentration of rest, the Taekwondo group was lower than the control group, but there was no significance. In LDH and CPK activity of rest, the Taekwondo group were significantly(p<.05, p<.01) higher than the control group. In the Taekwondo competition, lactate concentration and LDH, CPK activity in both of the group were all more significantly (p<.05, p<.01, p<.01) after the 3rd round than in the 1st and 2nd round. Putting the above-mentioned together, we can assume tat CPK is related to ATP-PC system, while lactate and LDH are connected with lactate system. In the future, I think that it will be necessary to find how to classify the ratio of ATP-PC system which was brought about n the Taekwondo competition, that of lactate system and aerobic on the metabolism.

Column-Switching HPLC法을 利用한 血淸中의 Ciprofloxacin의 定量法에 관한 硏究

李允中,李惠淑,禹炳虎 성균관대학교 약학연구소 1990 成均藥硏論文集 Vol.2 No.1

Abstracts-A small precolumn(40×2 mm, i.d.) packed with Amberlite XAD-7 facilitates the determination of ciprofloxacin in serum by permitting direct injection of relatively small volume(100㎕) of diluted serum. A pH 7.0 phosphate buffer washes out the serum components and acetonitrile: methanol: 5 mM tetrabutylammonium bromide(pH 2.0) (10 : 5 : 85, v/v) subsequently elutes the drug from the precolumn through the analytical column. This method showed high precision with good sensitivity and speed, and a detection limit of 0.1㎍/㎖. The total analysis time per sample was less than 15 min, and the mean coefficients of variation of intra- and inter-assay were both less than 4.8%. The method has been successfully applied to serum samples from human volunteers receiving oral administration of ciprofloxacin.

호산구성 간농양 54예의 임상상

강혜련,권혁수,송우정,이상민,김태범,박혜경,장윤석,김윤근,조상헌,민경업,김유영 대한알레르기학회 2004 천식 및 알레르기 Vol.24 No.2

두경부 편평상피암에서의 MIB-1, p53, bcl-2와 apoptosis의 발현 및 상호 연관성

손용준,한지용,윤형기,장수원,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Squamous cell carcinoma of the head and neck represents approximately 2.0∼4.0% of all malignant tumors, with an etiology closely associated with smoking, drinking and various genetic alterations. The purpose of this study was to investigate the correlation of MIB-1, p53, bcl-2 and apoptosis according to degree of differentitation of squamous cell carcinoma. Methods and Material : forty-eight cases of squamous cell carcinoma of the head and neck were selected for this study. MIB-1, p53, bc1-2 expression rate were studied by iromunogistochemical staining and the number of apoptosis observed by TUNEL method. Results : The expressioins of MIB-1, p53, bcl-2 and apoptosis were found in 56.3%, 50.0%, 14.6%, 85.4%, of 48 cases of squamous cell carcinoma, respectively. The expression of MIB-1, p53 and apoptosis were more higher in poorly differentiated cases than well differentiated cases. MIB-1 expression was related with p53 mutation (p<0.05) and apoptosis rate(p<0.05). Conclusion : From these results. the expressions of MIB-1, bcl-2 and apoptosis were related with degree of differentiation of squamous cell carcinoma and correlation among these parameters.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

법랑아세포종에서 apoptosis 연관 인자 발현과 apoptitic index

목동진,박진배,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Ameloblastoma is a common odontogenic benign tumor of the jaw bone. However, it might be able to infiltrate into the adjacent tissue, causing bony destruction and high recurrent rate. The aim of this study is to understand the biologic behavior of ameloblastoma through immunohistochemi cal stainings for apoptosis-related markers of bcl-2, box and caspase-3, examining the apoptosis index and simultanecusly. performing the same tests on MIB-1, which is a marker of cell-proliferationg capacity and p53, which is related with tumor malignancy, and also study the differences between the above marker's expressions and their relationships. Methods and Material : The 39 cases of ameloblastoma were used after the surgery conducted at the Pusan Back Hospital and the Pusan University Hospital during the period from January 1991 to June 2001. The clinical parameters were recorded by patent's age, sex, location of tumor, treatment modality, radiologic findings and recurrences. Based on hematoxylin & eosin findings, they were histologicaly subdivided into follicular and non-follicular. Immunohistochemical stainings for bcl-2, bax, caspase-3. p53 and MIB-1 and TdT-mediated dUTP-biotin nick end labelling method for apoptosis were performed and also statistical analyses were conducted between clinicopathologic parameters and expressions for bcl-2, bax, caspase-3, p53 and MIB-1 and apoptosis. Results : six(15.4%) recurrences were found out of 39 cases. For the patient's age of 40 and above, found a higher recurrence rate(p=0.0182) and multilocular on X-ray(p=0.0640), but there were no significant differences between recurrence rate, sex, location of tumor, treatment modality and histologic subtype. Apoptosis was found in 10(25.6%) of 39 cases and bcl-2 was(20.5%), bax was 21(53.8%), caspase-3 was 19(48.7%). the positive reaction for p53 was 13 cases(33.3%) and 5(12.8) cases for MIB-1. In the positive case of apoptosis and MIB=1, both of them have showed an increasing tendency of high recurrence, but there were no statistical significance. It showed no significant relationship between expression rates of bcl-2, bax, caspase-3 and p53 and recurrence rate. In follicular type, bcl-2 and p53 positive reaction revealed increasing tendency, however, no significant relationship between radiological finding, expressions of apoptosis, bax, caspase-3 and MIB-1 and histologic subtype has found. No significant relationship between apoptosis and apoptosis-related markers as bcl-2, bax, and caspase-3 expressions has found. There was a significant relationship between bcl-2 and p53 expressions(p<0.01), but no significant differences of apoptosis and expressions of bax and caspase-3 according to p53 and MIB-1 expressions have noted. Conclusion : Apoptosis-related bcl-2, bax, caspase-3 expressions and p53 and MIB-1 expressions are involved in the development of ameloblastoma and high recurrent rate is related to the age of more than 40 years, multichambered lesion and the positive reaction for apoptosis and MIB-1. These finding suggest that patient's age, radiologic findings, apoptosis and proliferation activity of ameloblastoma could be useful markers to predict recurrence.

만성 호중구성 백혈병 1예

박기령,조성민,우가은,이기현,손혜영,임정윤,최진혁,이순남,정화순 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.2

Chronic neutrophilic leukemia(CNL) is a very rare myeloproliferative disease, characterized by sustained mature neutrophilic leukocytosis with granulocytic bone marrow infiltration, high NAP(neutrophilic alkaline phosphatase) score and absence of philadelphia chromosome, It is frequently accompanied by hepatosplenomegaly, elevated serum vitamin B_12 and uric acid level. For the diagnosis of CNL, the leukemoid reaction, especially secondary to neoplasia, infection and autoimmune diseases, should be excluded. Since Tuohy's first description in 1920, more than 50 cases fullfilling the above criteria have been reported worldwide, and 4 cases in Korea. Several authors have demonstrated the defect of intracellualr killing in the mature neutrophil and this finding correlates well with the very high incidence of fatal infection, The hemorrhagic diasthesis in CNL is caused by functional abnormality of the platelet. This disease has tendency to transform to blastic crisis and acute leukemia as in other myeloproliferative disease, but characteristically shows frequent coexistence with multiple myeloma. Until now , the therapeutic trials in CNL have been disappointing. Hydroxyrea and busulfan can control hyperleukocytosis. On the basis of functional defect in neutrophil, alpha-2b-interferon has been tried and several reports have demonstrated the clinical and functional effect of interferon on CNL. CNL is very rate hematologic disease and there are few report about general aspect of disorder. We report here a typical CNL case presenting with splenomegaly and leukocytosis with a review of the literature.

Retinoic acid에 의해 유발된 흰쥐의 구개열에서 Transforming Growth Factor-beta 1, 2, 3의 발현의 변화

김종환,박진혁,신선호,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Craniofacial malformations are among the most frequent congenital birth defects in humans : cleft palate, that is inadequate fusion of the palatal shelves. Retinoic acid is teratogenic in many species, producing multiple malformations, including cleft palate. Also it plays an important role in embryogenesis, by regulating morphogenesis, cell proliferation, differentiation, and deposition of the extracellular matrix. The effects of retinoic acid which leads to cleft palate vary depending on the stage of development exposed. After exposure of embryonic mice to retinoic acid on gestational day(GD) 10, abnormally small palatal shelves form. And the elevation of palatal shelves is delayed. The growth and differentiation factor, transforming growth factor-beta(TGFβ) family is thought to play important roles in multiple developmental processes, especially, the development of the embryonic palate. The expression of the TGFβ family in embryonic palate suggests a functional role for these molecules. In this study, author has determined the tissue distributions of TGFβ family in murine embryonic palate, and compared them in terms of developmental stage and locations in normal and retinoic acid-induced cleft palate. Methods and Material : Retinoic acid was administered orally at gestational day(GD) 10 to ICR mice. The pregnant mice were sacrifeced on GD 12, 13. 14, 15 to obtain the fetuses and immunohistochemistry was performed. And using the scanning electron microscope, morphology of normal palate and cleft palate was studied. Results : In the retinoic acid-treated fetuses, palatal shelves did not elevate and cleft palate was induced. On GD 13 in the palates of the retinoic acid-treated fetuses, expression of the TGFβ1, 3 decreased dramatically, but that of TGFβ2, did not change. The expression of the TGFβ1, 3 in the palates of the retinoic acid-treated fetuses, increased on GD 14, 15, Significant differences in TGFβ family expression were observed with areas of the palate. Conclusion : These results suggest that retinoic acid inhibits the expression of TGFβ 1, 3, which is critical in the developing palate, and elevation of palatal shelves is delayed and impaired, therefore cleft palate is induced. These data demonstrate a role for retinoic acid and retinoic aicd-induced TGFβ family in the regulation of palatal development and suggest a role for TGFβ in retinoic acid-induced cleft palate.