3T Multi Voxel Spectroscopy에서 SENSE와 NEX 변화에 따른 정상인 뇌 대사물질 변화 분석

성열훈,임재동,이재현,조성봉,우동철,최보영,Seong, Yeol-Hun,Rhim, Jae-Dong,Lee, Jae-Hyun,Cho, Sung-Bong,Woo, Dong-Chul,Choe, Bo-Young 한국의학물리학회 2008 의학물리 Vol.19 No.4

자기공명분광법(magnetic resonance spectroscopy: MRS)은 인체내 대사물질을 정량분석하여 병변의 조기진단 및 정밀진단에 도움을 주고 있으며, 최근 임상에 이용되고 있는 자기공명분광법은 single voxel spectroscopy (SVS) 기법과 multi voxel spectroscopy (MVS) 기법이 있다. 본 연구에서는 SENSE와 NEX를 변화시킨 multi voxel spectroscopy (MVS)의 데이터와 기존 single voxel spectroscopy (SVS)의 데이터를 비교 분석하여, 각각의 데이터의 유의성 차이를 평가하고자 하였다. 정상 성인 지원자 13명(남자: 5명, 여자: 8명, 평균 41세, 표준편차 11.65세)을 대상으로 chemical shift image (CSI)를 이용한 MVS검사를 시행하였다. 장비는 3.0T Achieva Release Version 2.1 (Philips Medical System, Netherland)을 이용하였고, 8 channel head coil을 사용하여 brain thalamus 부위에서 CSI spectrum을 1 slice 획득하였다. Scan parameter로는 FOV (field of view): $230{\times}184mm^2$, TR (time to repetition): 2000 msec, TE (time to echo): 288 msec, matrix: $15{\times}12$, VOI(view of interest): $110{\times}110mm^2$, voxel size: $15{\times}15{\times}15mm^3$로 하였다. SENSE factor (S)와 NEX (N)는 S1*N1, S2*N1, S2*N2, S3*N2로 변화하여 스펙트럼을 획득하였고, 각 scan time은 5분 54초, 3분 32초, 6분 20초, 4분 20초였다. 얻은 모든 MRS 데이터는 jMRUI 3.0 Version 프로그램에서 분석하였고, SENSE factor와 NEX를 변화시켜 얻은 MVS data 그룹들이 정상 성인 뇌 대사물질의 변화에 영향을 주는지 검증하기 위해 그룹 간에 ANOVA분석을 실행하여 P 값이 0.05보다 크게 나오면 그룹들 사이에 유의한 차이가 없다고 분석하였다. NAA/Cr과 Cho/Cr의 상대적 비율은 MV와 SVS사이에서는 유의한 차이가 없었다. 즉, SENSE factor와 NEX를 변화시켜 얻은 MVS data에서 정상 성인 뇌조직의 대사물질의 변화를 관찰한 결과, S1*N1의 NAA/Cr은 $1.45{\pm}0.03$, Cho/Cr은 $0.88{\pm}0.03$이고, S2*N1의 NAA/Cr은 $1.44{\pm}0.03$, Cho/Cr은 $0.87{\pm}0.05$, S2*N2의 NAA/Cr은 $1.43{\pm}0.02$, Cho/Cr은 $0.87{\pm}0.04$이며, S3*N2의 NAA/Cr은 $1.45{\pm}0.03$, Cho/Cr은 $0.87{\pm}0.03$으로 나타났다(F-value : 1.37, D.F : 3, P-value : 0.262). 그러나 데이터의 질을 측정하기 위한 MVS 데이터의 NAA Peak line-width는 SVS 데이터의 NAA Peak line-width 보다 약 3배 정도 넓었다. 본 연구에서는 MVS에서 SENSE factor와 NEX 값을 다양하게 변화시킨 MVS의 데이터와 SVS의 데이터가 큰 차이가 없음을 확인하였다. 즉, 어는 특정 부위의 뇌 조직의 대사물질은 MVS와 SVS 기법 모두 큰 차이가 없음을 확인할 수 있었다. 그러므로 MVS는 SVS보다 광범한 부위를 짧은 시간 안에 검사할 수 있으므로 매우 유용한 방법이라고 사료된다. To evaluate the metabolic changes in normal adult brains due to alterations SENSE and NEX (number of excitation) by multi voxel MR Spectroscopy at 3.0 Tesla. The study group was composed of normal volunteers (5 men and 8 women) with a mean ($\pm$ standard deviation) age of 41 (${\pm}11.65$). Their ages ranged from 28 to 61 years. MR Spectroscopy was performed with a 3.0T Achieva Release Version 2.0 (Philips Medical System-Netherlands). The 8 channel head coil was employed for MRS acquisition. The 13 volunteers underwent multi voxel spectroscopy (MVS) and single voxel spectroscopy (SVS) on the thalamus area with normally gray matter. Spectral parameters were as follows: 15 mm of thickness; 230 mm of FOV (field of view); 2000 msecs of repetition time (TR); 288 msecs of echo time (TE); $110{\times}110$ mm of VOI (view of interest); $15{\times}15{\times}15$ mm of voxel size. Multi voxel spectral parameters were made using specially in alteration of SENSE factor (1~3) and 1~2 of NEX. All MRS data were processed by the jMRUI 3.0 Version. There was no significant difference in NAA/Cr and Cho/Cr ratio between MVS and SVS likewise the previous results by Ross and coworkers in 1994. In addition, despite the alterations of SENSE factor and NEX in MVS, the metabolite ratios were not changed (F-value : 1.37, D.F : 3, P-value : 0.262). However, line-width of NAA peak in MVS was 3 times bigger than that in SVS. In the present study, we demonstrated that the alterations of SENSE factor and NEX were not critically affective to the result of metabolic ratios in the normal brain tissue.

Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice

Cho, Kyu-Hyuk,Cho, Jae-Woo,Song, Chang-Woo Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 mg/kg ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in $G_1,\;G_2\;and\;G_3$ mice. One hundred thirty five $G_1$ phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen $G_3$ recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.

Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice

Kyu-Hyuk Cho,Jae-Woo Cho,Chang-Woo Song 한국독성학회 2008 Toxicological Research Vol.24 No.1

N-ethyl-N-nitrosoures (ENU) is effective in inducing hypermorphic mutation as well as hypomorphic and antimorphic mutations. Therefore, this mutagen is used to the production of mutant in the mice. In order to perform an effective ENU mutagenesis using BALB/cAnN mice, determination of optimal dosage and dosage regimen of ENU is necessary. And this study tried to develop a suitable screening method and searched for novel and various mutants as model animals in phenotypedriven ENU mutagenesis. We have carried out dosage regimen for mutagenizing dose of 200 ㎎/㎏ ENU in the BALB/c mice. Total screened mice were 30,133. As the results of Esaki and Cho's Phenotype Screening, we got 2,516 phenotypic and behavior abnormalities in G₁, G₂and G₃mice. One hundred thirty five G₁phenodeviants were tested for inheritance and 16 dominant mutants were discovered. Forty two recessive mutants were also found in tested 201 micropedigrees. Early-onset mutant mice included the dysmorphology of face, eye, tail, limb, skin, and foot and abnormal behavior like circling, swimming, head tossing, stiff-walking, high cholesterol level, and tremor etc. In this study we could effectively screen G₃recessive mutants. The frequent and concise early-onset screening before weaning will be available for ENU mutagenesis.

Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

노사학파 심포(心圃) 문형(文炯)의 학술 관계망

조우진 ( Cho Woo-jin ) 전남대학교 호남학연구원 2023 호남학 Vol.- No.74

본 논문은 노사학파 재전문인 심포(心圃) 문형(文炯)의 학술 관계망을 살펴보는 데 목적이 있다. 문형은 전남 화순 청풍면 출신으로 개항기와 일제강점기를 걸쳐 살았던 성리학자이다. 어려서는 민우식(閔佑植)에게 13년 동안 유학을 배웠으며, 민우식이 죽기 직전 정의림(鄭義林)을 찾아가 9년 동안 기정진(奇正鎭)의 이론을 수학하였다. 정의림이 죽자, 『노사집(蘆沙集)』을 스승으로 삼아 노사학의 정수를 확인하였다. 문형은 영ㆍ호남의 노사학파와 화서학파의 재전문인 뿐만 아니라 영남의 한주 학파의 재전문인 및 당대 최고의 문장가 등과 교류를 확대하면서 학문적 유대와 사상적 통합을 이루고자 했다. 이것이 바로 지역과 사상을 횡단하는 문형의 학술 관계망의 특징이다. 특히 문형은 자신의 호를 ‘심포(心圃)’라고 했던 것처럼 성리학의 핵심 개념인 심(心)을 바탕으로 자신의 철학 체계를 완성하였다. 그는 화서학파 김평묵(金平默) ㆍ최익현(崔益鉉)의 문인들과 교유하면서 이항로(李恒老)의 심주리(心主理)에 대한 견해를 적극적으로 옹호하였으며, 한주학파 곽종석(郭鍾錫)의 문인들과 소통하면서 이진상(李震相)의 심즉리(心卽理)를 비판적으로 수용하였다. 더불어 문형은 리(理) 중심의 사유체계를 바탕으로 <성사심제변(性師心弟辨)>을 지어 간재(艮齋) 전우(田愚)가 심을 주기적(主氣的) 입장으로 보는 병폐를 지적하였다. 문형의 위상은 다양한 학파와 심을 중심으로 한 학술 관계망을 형성하여 당시의 성리학적 문제의식을 통합하고자 했다는 점이고, 그의 역할은 화순 노사 학맥을 이어 확산을 주도했을 뿐만 아니라, 화순 노사학파의 중심에 서 있었다는 점이다. 문형은 정주학을 토대로 노사학을 전수하여 화순 근현대 유학을 정립하는 데 중추적인 역할을 하였으며, 미래지향적 지역학으로써 화순학(和順學)을 수립할 수 있는 학술적 기반을 제공하였다. This paper aims to explore the academic network of Shimpo[心圃] MunHyeong(文炯, 1882-1936), a redeliver disciple[再傳門人] of Nosa School[蘆沙學派]. MunHyeong is a Neo-Confucian scholar who was born in Cheongpung-myeon[淸風面], Hwasun, Jeollanam-do, and lived during the opening period and Japanese colonial era. When young, he learned to study Neo-Confucianism from MinWoosik[閔佑植] for 13 years, and just before Min Woo-sik died, he visited JungEuirim[鄭義林] and studied KiJeongjin[奇正鎭]’s theory for 9 years. When JungEuirim died, he confirmed the essence of Nosahak[蘆沙學] regarding 『Nosajip[蘆沙集]』 as his teacher. MunHyeong sought to achieve academic ties and ideological integration by expanding exchanges with not only redeliver disciples[再傳門人] of Nosa School[蘆沙學派] and Hwaseo School[華西學派] in YeongnamㆍHonam but also redeliver disciples[再傳門人] of the Hanju School[寒洲學派] and Good writers[文章家] of the time. This is the characteristic of academic network of MunHyeong that crosses regions and ideas. In particular, MunHyeong completed his philosophical system based on the core concept Shim[心] of Neo-Confucianism, as he called his pen name ‘Shimpo’. He actively advocated IHanglao [李恒老]’s view of Shimjuli[心主理] while interacting with the writers of the Hwaseo School of KimPyeongmuk[金平默] and ChoeIckhyun[崔益鉉], and critically accepted IJinsang[李震相]’s Shimjeukli[心卽理] while communicating with the writers of KwakJongseok[郭鍾錫] of the Hanju School. In addition, Moon Hyung wrote < Seongsashimjebyeon[性師心弟辨] > based on the Li[理]-centered thinking system and pointed out the problem of Ganje[艮齋] JeonWoo[田愚]'s view of Shim[心] as Jugi[主氣] position. MunHyeong's status was to form an academic network centered on various schools and Shim and to integrate the Neo-Confucian problem consciousness of the time, and his role was not only to lead the spread of Hwasun Nosahakmaek, but also to be at the center of Hwasun Nosa School. MunHyeong played a pivotal role in establishing modern and contemporary Hwasun Confucianism by transferring Nosahak based on ChéngZhūxué[程朱學], and provided an academic foundation for establishing Hwasunhak[和順學] as a future-oriented regional studies.

단어출현빈도분석과 AHP를 통한 기술 연구 동향과 기대 효과 분석: 바이오 에너지 분리막을 중심으로

이인우(Lee, In-Woo),서한결(Seo, Han-Gyeol),장수진(Jang, Soo-Jeen),조철희(Cho, Churl-Hee),정양헌(Chung, Yang-Hon) 한국경영교육학회 2020 경영교육연구 Vol.35 No.5

[연구목적] 본 연구는 과학 기술 연구 동향과 기술 도입 기대 효과를 함께 분석하는 방안을 바이오 에너지 분리막 분야에 적용하여 연구개발 전략 제시에 도움이 되는 연구 방안을 제시하고자 하였다. [연구방법] 본 연구는 분리막 기술 연구 방향과 기대효과를 분석하기 위해 각각에 대해 두 가지 연구방법을 이용하였다. 우선 텍스트 마이닝을 통해 단어출현 빈도분석으로 선행연구의 연구 방향을 확인하였고, AHP를 통해 기대효과를 알아보았다. [연구결과] 분석결과 분리막 기술의 연구 방향은 주로 분리막 관련 다양한 공정과 공정개선을 통한 성능 향상에 있는 것으로 확인되었다. 분리막 기술을 통한 기대 효과는 바이오 에너지 공정에서 발생하는 환경 문제 해결이 주된 것으로 나타났다. [연구의 시사점] 분리막 관련 연구는 분리막 성능에 중점을 두고 진행되고 있으며 분리막을 실제 공정에 도입하는 측에서 기대하는 부분은 분리막 도입으로 인한 효과와 분리성능 향상에 의한 효과에 중점을 두고 있음을 확인하였다. 이는 분리막 기술 연구가 실제 니즈를 잘 반영하여 이루어지고 있음을 보여준다. 이처럼 특정 기술의 연구 방향과 도입에 따른 기대효과를 함께 분석할 수 있으며, 이는 연구개발 전략 제시에 도움이 될 수 있음을 확인하였다. [Purpose] Purpose of this study is to propose a research methodology that is helpful in presenting the R&D strategy by applying analysis on research trends and expected effect of technology introduction together in the field of bioenergy membranes. [Methodology] For this purpose, we used two research methodologies. Word frequency analysis of text mining was used to analyze the research trends of previous studies, and the expected effect was investigated through AHP. [Findings] This study found that the research trends of membrane is mainly to improve performance through various processes and expected effect focus on environmental problem solving arising from bioenergy process. [Implications] It was confirmed that main research trends of membrane is performance of membrane and expected effect is performance improvement by introduction of membrane in bioenergy processes. This shows that research on membrane is well reflected in actual needs. This methodology can be helpful in presenting the consumer-oriented R&D strategy.

Three-Dimensional Porous Collagen/Chitosan Complex Sponge for Tissue Engineering

Kim, Sung Eun,Cho, Yong Woo,Kang, Eun Jung,Kwon, Ick Chan,Lee, Eunhee Bae,Kim, Jung Hyun,Chung, Hesson,Jeong, Seo Young The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

자색고구마가 사염화탄소 투여에 의한 흰쥐의 간손상 보호에 미치는 영향

조영자,김현아,방미애,오용비,정병춘,문윤호,정우진 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.3

The purpose of this study was to investigate the effects of dietary purple sweet potato(lpomoea batatas) powder on serum lipid levels and antioxidative enzymes in normal and pretective effect on hepatotoxicity rats induced by carbon tetrachlolide. Four groups of rats (3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(C)_(1) induced hepatotoxicity rats fed control diet(EC)_(1) normal rats fed purple sweet potato diet(P), and induced hepatotoxicity rats fed purple potato sweet diet(EP). Rats were induced by single injection of 50% carbon tetrachloide(0.1 mL/100 g B.W., i.p.). The rats were fed ad libitum each of the experimental diet for 5 weeks. After 5 weeks the rats were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver homogenates. But serum concentrations of lipid was not significant in all groups. Serum alanine aminotransferase(ALT/GPT) and aspartate aminotransferase(ALT/GPT)of the EC and EP groups were heigher than the C and P groups. The hepatic glucose 6-phosphatase(G9Pase) activity of the group fed purple potato diet(P) was lower than the other groups(p<0.05). However, The glutathione peroxidase(GPx) activities was not statistically different between the groups. Renal glutathione S-transferase(GST) activity of the EC and EP groups were lower than the C and P groups(p<0.05). In conclusion, these results suggest that purple sweet potato is believed to be possible protective effect on hepatotoxicity rats induced by carbon tetrachlolide.

처리기 메쉬를 이용한 정규화된 재귀규칙의 증명-이론적 의미 계산

조우현,박승섭 釜慶大學校 1998 釜慶大學校 論文集 Vol.3 No.2

A deductive database consists of facts stored in the extensional database and rules representing the intensional database. Because of the difficulty of evaluating rules, many parallel evaluation algorithms for rules have been presented. This paper proposes a new paradigm to evaluate linear recursion rules which contain transitive dependency by using a mesh of processors. An evaluation of normalized rules is a computation of the proof-theoretic meaning of a collection of rules. The normalized recursion rule which contains transitive dependency is defined, the existence of an equivalent expression for the rule is proved, a paradigm using transitive closure operations for parallel evaluation of normalized rule based on the equivalent expression is proposed, and the comparative performances of the parallel evaluation algorithms are presented.