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Helical tomotherapy for spine oligometastases from gastrointestinal malignancies
Choi, Yun-Seon,Kim, Jun-Won,Lee, Ik-Jae,Han, Hee-Ji,Baek, Jong-Geal,Seong, Jin-Sil The Korean Society for Radiation Oncology 2011 Radiation Oncology Journal Vol.29 No.4
Purpose: This study evaluated the treatment effectiveness and proper radiation dose of helical tomotherapy (HT) in spine oligometastases from gastrointestinal cancers. Materials and Methods: From 2006 to 2010, 20 gastrointestinal cancer patients were treated with HT for spine oligometastases (31 spine lesions). The gross tumor volume (GTV) was the tumor evident from magnetic resonance imaging images fused with simulation computed tomography images. Clinical target volume (CTV) encompassed involved vertebral bodies or dorsal elements. We assumed that the planning target volume was equal to the CTV. We assessed local control rate after HT for 31 spine metastases. Pain response was scored by using a numeric pain intensity scale (NPIS, from 0 to 10). Results: Spine metastatic lesions were treated with median dose of 40 Gy (range, 24 to 51 Gy) and median 5 Gy per fraction (range, 2.5 to 8 Gy) to GTV with median 8 fractions (range, 3 to 20 fraction). Median biologically equivalent dose (BED, ${\alpha}/{\beta}$ = 10 Gy) was 52 $Gy_{10}$ (range, 37.5 to 76.8 $Gy_{10}$) to GTV. Six month local control rate for spine metastasis was 90.3%. Overall infield failure rate was 15% and outfield failure rate was 75%. Most patients showed pain relief after HT (93.8%). Median local recurrence free survival was 3 months. BED over 57 $Gy_{10}$ and oligometastases were identified as prognostic factors associated with improved local progression free survival (p = 0.012, P = 0.041). Conclusion: HT was capable of delivering higher BED to metastatic lesions in close proximity of the spinal cord. Spine metastases from gastrointestinal tumors were sensitive to high dose radiation, and BED (${\alpha}/{\beta}$ = 10 Gy) higher than 57 $Gy_{10}$ could improve local control.
Electron Donating Ability of MeOH Extracts from Three Korean Mistletoes
Choi, Won Sil,Ahn, Won Yung 한국목재공학회 2000 목재공학 Vol.28 No.4
This experiment was accomplished to investigate antioxidative activity of Korean mistletoe by organic solvents partitioning of methanol extact of 3 Korean mistletoes, fractionationing on column chromatography, and evaluation the reduction of a free radical α,α-diphenyl-β-picrylhydrazyl. Butanol partition of Loranthus yadoriki MeOH extract showed higher electron donating ability than α-tocopherol. It was thought phenolic compounds including gallic acid account for antioxidative activity, on execution sub-fractionation, electron donating ability evaluation, and GC/MS analysis, but further studies on what are major actives must be investigated exactly.
Choi, In-Sil,Kim, Dae-Hyuk,Lee, Chang-Won,Kim, Jae-Won,Chung, Young-Ryun The Korean Society for Microbiology and Biotechnol 1998 Journal of microbiology and biotechnology Vol.8 No.5
Random amplified polymorphic DNA (RAPD) markers were used to survey genetic variability among 34 Botrytis cinerea isolates from nine different host plants in Korea. For RAPD analysis, 115 arbitrary decamer primers were initially screened for polymorphic major DNA bands with 11 representative B. cinerea isolates. Eleven primers that initially detected polymorphisms were tested a second time with additional 23 isolates of B. cinerea as well as one isolate of Botrytis squamosa as an outgroup. The RAPD analyses revealed that all isolates except one showed different molecular phenotypes. Dendrograms obtained from dissimilarity matrices using the unweighted paired group method of arithmetic means (UPGMA) showed the 36.4% to 90.0% similarity among all B. cinerea isolates. The B. squamosa isolate showed the least similarity to all B. cinerea isolates. The cluster analyses indicated no correlation among all the characteristics examined including molecular phenotypes, host and geographic origins, year of isolation, or pathogenicity. The RAPD data suggest that a high level of genetic variation exists among Korean populations of B. cinerea and it seems to be caused by heterokaryosis among preexisting molecular phenotypes.
( Won-sil Choi ),( Seung-ok Yang ),( Ji-hyun Lee ),( Eun-ji Choi ),( Yun-hee Kim ),( Jiyoon Yang ),( Mi-jin Park ) 한국목재공학회 2020 목재공학 Vol.48 No.5
This study was aimed to investigate the changes of chemical composition of the volatile organic compounds (VOCs) emitted from red pine needles in the process of needle abscission or senescence. The VOCs in intact, senescent, and litter red pine needle samples were analyzed by headspace-solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC/MS). And then, multivariate statistical interpretation of the processed data sets was conducted to investigate similarities and dissimilarities of the needle samples. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to investigate the dataset structure and discrimination between samples, respectively. From the data preview, the levels of major components of VOCs from needles were not significantly different between needle samples. By PCA investigation, the data reduction according to classification based on the chlorophyll a / chlorophyll b (Ca/Cb) ratio were found to be ideal for differentiating intact, senescent, and litter needles. The following OPLS-DA taking Ca/Cb ratio as y-variables showed that needle samples were well grouped on score plot and had the significant discriminant compounds, respectively. Several compounds had significantly correlated with Ca/Cb ratio in a bivariate correlation analysis. Notably, the litter needles had a higher content of oxidized compounds than the intact needles. In summary, we found that chemical compositions of VOCs between intact, senescent, and litter needles are different each other and several compounds reflect characteristic of needle.
Choi, Won-Sik,Nam, Seok-Woo,Kim, Il-Doo,Kim, Seung-Han,Park, Kun-Ho,Bae, In-Kyung,Park, Eun-Sil,Jeon, Hwang-Ju,Lee, Sung-Eun Hindawi Limited 2015 Journal of chemistry Vol.2015 No.-
<P>Pesticidal activities of 4-[5-(2-cyclopropylaminopyrimidin-4-yl)-4-(4-chloro-2-fluoro-phenyl)thiazol-2-yl]-1-methylpiperidine, designated as Comp I, have been determined against a mosquito larva,<I>Culex pipiens pallens</I>, and a phytopathogenic fungus,<I>Phytophthora capsici</I>. Comp I was used as the leading compound in this study. The compounds were synthesized by reacting them with two functional groups, 3-thiophenyl and 2-thiophenyl groups, instead of 4-chloro-2-fluorophenyl group in Comp I. Other functional groups such as 2-aminothiazole, 2-(1-methylpiperazin-4-yl)thiazole, and 2-(piperazin-4-yl)thiazole were also introduced instead of 2-methylpiperidin-4-yl-thiazole of Comp I. Compounds designated as XIII-6~XV-7 were newly synthesized and their structures were confirmed by<SUP>1</SUP>H- and<SUP>13</SUP>C-NMR spectroscopy. Mosquito larvicidal activities of all the synthesized compounds against<I>C. pipiens pallens</I>were examined and Comp I among them showed the strongest larvicidal activity as 0.513 mM of LC50value. The fungicidal activities of all the synthesized compounds against<I>P. capsici</I>were examined using the whole plant method. Among the XIII-6~XV-7 chemicals, 5-(2-cyclopropylaminopyrimidin-4-yl)-4-(thiophen-2-yl)thiazol-2-amine (VIII-6) showed the most potent antifungal activity<I>in vivo</I>. While the EC50value of the commercial fungicide dimethomorph was 4.26 <I>μ</I>M, EC50of VIII-6 was 0.94 <I>μ</I>M. Therefore, thiazole derivatives can be considered as viable candidates for the control of mosquito larvae and plant diseases.</P>
Choi, Yong Jun,Kim, Yae-Jean,Yong, Dongeun,Byun, Jung-Hyun,Kim, Taek Soo,Chang, Yun Sil,Choi, Min Ji,Byeon, Seung Ah,Won, Eun Jeong,Kim, Soo Hyun,Shin, Myung Geun,Shin, Jong Hee U.S. Department of Health and Human Services * Cen 2018 Emerging Infectious Diseases Vol.24 No.9
<P>We recently observed the emergence of fluconazole-resistant <I>Candida parapsilosis</I> bloodstream isolates harboring a Y132F substitution in Erg11p in South Korea. These Y132F isolates had a higher propensity to cause clonal transmission than other fluconazole-resistant isolates and persisted within hospitals for several years, as revealed by microsatellite typing.</P>
Hypoxia-induced Angiogenesis during Carcinogenesis
Choi, Kyu-Sil,Bae, Moon-Kyoung,Jeong, Joo-Won,Moon, Hyo-Eun,Kim, Kyu-Won 한국생화학분자생물학회 2003 BMB Reports Vol.36 No.1
The formation of new blood vessels, angiogenesis, is an essential process during development and disease. Angiogenesis is well known as a crucial step in tumor growth and progression. Angiogenesis is induced by hypoxic conditions and regulated by the hypoxia-inducible factor 1(HIF-1). The expression of HIF-1 correlates with hypoxic-induced angiogenesis as a result of the induction of the major HIF-a target gene, vascular endothelial cell growth factor (VEGF). In this review, a brief overview of the mechanism of ahgiogenesis is discussed, focusing on the regulatory processes of the HIF-1 transcription factor. HIF-1 consists of a constitutively expressed HIF-1 beta HIF-1β subunit and an oxygen-regulated HIF-1 alpha (HIF-1α) subunit. The stability and activity of HIF-1α are regulated by the interaction with various proteins, such as pVHL, p53 and p300/CBP as well as by post-translational modification, hydroxylation, acetylation, and phosphorylation. It was recently reported that HIF-1α binds a co-activator of the AP-1 transcription factor, Jab-1, which inhibits the p53-dependent degradation of HIF-1 and enhances the transcriptional activity of HIF-1 and the subsequent VEGF expression under hypoxic conditions. ARDI aceylates HIF-1α and stimulates pVHL-mediated ubiquitination of HIF-1α. With a growing knowledge of the molecular mechanisms in this field, novel strategies to prevent tumor angiogenesis can be developed, and from these, new anticancer therapies may arise.
Choi, Su-La,Choi, Yun-Sil,Kim, Young-Kwan,Sung, Nack-Do,Kho, Chang-Won,Park, Byong-Chul,Kim, Eun-Mi,Lee, Jung-Hyung,Kim, Kyung-Mee,Kim, Min-Yung,Myung, Pyung-Keun 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrix-assisted laser desorption/ionization-time of flight (MALDl- TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-κB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific eye/in D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic aoents aqainst the SK-MEL-28 human melanoma cell line.