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Agrobacterium-mediated Seed Transformation System in Garland Chrysanthemum
Chae, Won-Byoung,Choi, Geun-Won,Chung, In-Sik 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-
Agrobacterium을 이용한 쑥갓 종자 형질전환이 수행되었다. 상처 처리한 종자와 무처리 종자를 terpene cyclase 유전자가 재조합된 pILTAB vector를 갖는 Agrobacterium tumefaciens LBA4404와 5일 동안 공동배양하였고 공동배양한 종자들은 kanamycin 172 μM과 cefotaxime 420 μM이 첨가된 선발배지에서 배양되었다. 상처 처리한 종자는 무처리 종자에 비하여 높은 발아율을 보였고 상처 처리한 종자의 유묘가 형질전환 된 것으로 추정되는 식물체를 생산한 반면에 무처리한 종자의 유묘는 선발배지에서 고사하였다. Terpene cyclase 유전자 특이 primer를 이용한 PCR 분석을 실시한 결과, 선발배지에서 생장한 7개 중 1개의 쑥갓에서 1.64 kb 크기의 terpene cyclase 유전자의 성공적인 도입을 확인하였다. A transgenic garland chrysanthemum plant was successfully produced through Agrobacterium-mediated seed transformation system. Both intact and cracked seeds were co-cultivated for 5 days with A. tumeficiens strain LBA4404 carrying binary vector pILTAB 357 including 1.64 kb terpene cyclase gene isolated from sweet annie (Artemisia annua L.) and cultured on selection medium supplemented with 172 μM of kanamycin and 420 μM of cefotaxime to select putative transgenic seedlings. Germination rate of cracked seeds was higher than that of intact seeds and seedlings from cracked seeds developed into putative transgenic plants whereas seedlings from intact seeds were blighted on the selection medium. Results from PCR analysis with terpene cyclase gene-specific primers confirmed a successful insertion of 1.64 kb terpene cyclase gene into 1 seedling among 7 seedlings grown on the selection medium.
Plant Regeneration Depending on Explant Type in Chrysanthemum coronarium L.
Chae Won-Byoung,Choi Geun-Won,Chung In-Sik The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.4
Plant regeneration depending on explant type was inves-tigated with cotyledon, hypocotyl, and leaf explants of garland chrysanthemum (Chrysanthemum coronarium L.) cultured on MS basal medium supplemented with various concentrations of SAP and NAA combination. Among the three different types of explants, hypocotyl explants grown on MS medium containing $1.0{\mu}M\;NAA,\;1.0{\mu}M\;BA\;and\;6{\mu}M\;AgNO_3$ produced the highest adventitious shoots (4.67 per explant). Hypocotyl explants not only produced more vigorous shoots, which regenerated aster than the cotyledon and leaf explants. An efficient root formation was observed in MS medium containing $3\%$sucrose. The concentration of NAA did not show significant effects on root formation. Results from this experiment suggested that hypocotyl explants were efficient for the regeneration of garland chrysanthemum.
Chae, Hee-Sung,You, Byoung Hon,Choi, Junjeong,Chin, Young-Won,Kim, Hojun,Choi, Han Seok,Choi, Young Hee ELSEVIER SCIENCE B.V.; AMSTERDAM 2019 JOURNAL OF FUNCTIONAL FOODS Vol.62 No.-
<P><B>Abstract</B></P> <P>Ginseng berry extract contains plenty of ginsenosides showing anti-obesity and anti-hyperglycemic activities. Regarding that long-term intakes of herbal products to control metabolic disorders as popular alternatives have become popular, ginseng berry extract plus metformin is an attractive item for long-term glycemic control. Herein, we investigated the effect of ginseng berry extract (GBE) or its combination with metformin against obesity and hepatic steatosis in high-fat diet fed mice.</P> <P>GBE altered abnormal features (e.g. body weight, serum profiles, hepatic steatosis/inflammation and fat mass). Concomitantly, GBE increased mRNA levels of adiponectin and CCAAT/enhancer binding protein. All these changes were observed in metformin alone and GBE plus metformin. Moreover, in GBE plus metformin intake, GBE increased organic cation transporter-mediated metformin uptake in liver and mRNA of AMP-activated protein kinase together, which additively ameliorated obesity and hepatic steatosis. Therefore, GBE itself and GBE plus metformin could be useful to regulate metabolic disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GBE ameliorated obesity and hepatic steatosis in mice fed high-fat diet. </LI> <LI> GBE increased organic cation transporter-mediated metformin uptake in liver. </LI> <LI> GBE plus metformin additively ameliorated obesity and hepatic steatosis. </LI> <LI> GBE itself and GBE plus metformin could be useful to regulate metabolic disorders. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>In GBE plus metformin treatment, GBE plus metformin showed the additive efficacy against obesity, hepatic steatosis and hyperglycemia!</P> <P>[DISPLAY OMISSION]</P>
Massive Hemoptysis due to Acute Mitral Regurgitation with Sporadic Primary Mitral Valve Prolapse
Won Young Chae,Sun Jin Park,Chi Woo Song,Byoung Hoon Lee,Sang Hoon Kim,Jae Hyung Lee 대한중환자의학회 2015 Acute and Critical Care Vol.30 No.3
The most common cardiac cause of massive hemoptysis is mitral stenosis. Mitral regurgitation is rarely complicated by massive hemoptysis. A 48-year-old man with no significant medical history was admitted to our hospital with hemoptysis and production of 500 mL of blood within 24 hours. A pan-systolic murmur was found on chest examination. A chest computed tomography showed airspace consolidation in the right upper and middle lobes, with faint bilateral ground glass opacity. Echocardiography revealed mitral valve prolapse and grade IV mitral regurgitation. The patient was diagnosed with sporadic primary mitral valve prolapse. After mitral valve repair surgery, the patient recovered fully.
Development of Agrobacterium-mediated Transformation System in Garland Chrysanthemum
Chae, Won-Byoung,Choi, Geun-Won,Lee, Jung-Myung 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-
쑥갓에서의 효율적인 Agrobacterium을 이용한 형질전환 체계를 개발하기 위하여 일련의 실험이 수행되었다. 쑥갓의 잎절편체를 이용한 항생제 감응성 검정 결과 86 μM 이상의 kanamycin 농도가 형질전환 신초의 선발에 적당하였고 840 μM의 cefotaxime이 잎절편체의 생장에 부정적인 영향을 주지 않으면서 배지 내 Agrobacteria를 제거하기에 적절한 수준으로 선발되었다. Terpene cyclase 유전자가 재조합된 pILTAB vector를 갖는 Agrobacterium tumefaciens LBA4404와 5일간의 공동배양 후에 잎 절편체들은 NAA 2.5 μM, BAP 2.5 μM, AgNO_(3) 18 μM, kanamycin 86 μM 그리고 cefotaxime 840 μM이 함유된 선발배지에 배양되었다. 형질전환된 것으로 여겨지는 신초들이 발생하였고 발근을 위하여 sucrose 3% 와 kanamycin 86 μM이 첨가된 MS 배지에 옮겨졌다. 발근된 형질전환체들을 대상으로 terpene cyclase 유전자 특이 primer를 이용한 PCR 분석이 실시되어 재분화 쑥갓 내로 1.64 kb 크기의 terpene cyclase 유전자의 성공적인 도입을 확인하였다. A series of experiments were conducted to develop an efficient Agrobacterium-mediated transformation system in garland chrysanthemum (Chrysanthemum coronarium L.). The result from kanamycin sensitivity test using leaf explants of garland chrysanthemum suggested that the concentration above 86 μM of kanamycin could be an appropriate level to make a selection on transgenic shoots possible. Eight hundred forty μM of cefotaxime was selected as an effective level to remove Agrobacteria from the media without any negative effect on the growth of leaf explants. After 5 days of cocultivation with A. tumefaciens harboring the terpene cyclase gene in the binary vector pILTAB357, the leaf explants were cultured on the selection media containing 2.5 μM NAA, 2.5 μM BAP, 18 μM AgNO_(3), 86 μM kanamycin, and 840 μM cefotaxime. Putative transgenic shoots were generated and transferred to MS media supplemented with 3% sucrose and 86 μM kanamycin for root formation. Putative transgenic plants with roots were obtained and the result from PCR analysis with terpene cyclase gene specific primers showed successful insertion of 1.64 kb terpene cyclase gene into the genomic DNA of 7 plants among 8 putative plants obtained.