<i>Terrabacter koreensis</i> sp. nov., isolated from soil of a flowerbed

Won, KyungHwa,Kook, MooChang,Yi, Tae-Hoo International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.10

<P>A Gram-staining-positive, non-spore-forming, rod-shaped, non-motile and aerobic bacterium, strain THG-e54<SUP>T</SUP>, was isolated from soil of a flowerbed in Bucheon, South Korea. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. 16S rRNA gene sequence analysis revealed a clear affiliation of strain THG-e54<SUP>T</SUP> with the genus <I>Terrabacter</I>. Strain THG-e54<SUP>T</SUP> showed the closest phylogenetic relationship with <I>Terrabacter tumescens</I> DSM 20308<SUP>T</SUP> with a 16S rRNA gene sequence similarity of 98.6 %. The G+C content of the genomic DNA was 71.0 mol%. The major cellular fatty acids of strain THG-e54<SUP>T</SUP> were iso-C<SUB>15 : 0</SUB> and iso-C<SUB>16 : 0</SUB>, and the predominant menaquinone was menaquinone MK-8(H<SUB>4</SUB>). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified amino-containing phosphoglycolipid, unidentified phospholipids, an unidentified aminolipid and an unidentified aminophospholipid. The cell-wall peptidoglycan type was <SMALL>ll</SMALL>-diaminopimelic acid and the cell-wall sugars were glucose and ribose. DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 35.2 %) between strain THG-e54<SUP>T</SUP> and its five closest relatives. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-e54<SUP>T</SUP> could be clearly distinguished from closely related recognized members of the genus <I>Terrabacter</I>. Therefore the results of this study indicated the existence of a novel species of the genus <I>Terrabacter</I> , for which we propose the name <I>Terrabacter koreensis</I> sp. nov., with strain THG-e54<SUP>T</SUP> ( = KACC 17589<SUP>T</SUP> = JCM 19342<SUP>T</SUP>) as the type strain.</P>

Pioneering Air Quality Monitoring over East and Southeast Asia with the Geostationary Environment Monitoring Spectrometer (GEMS)

( Kyunghwa Lee ),( Dong-won Lee ),( Lim-seok Chang ),( Jeong-ah Yu ),( Won-jin Lee ),( Kyoung-hee Kang ),( Jaehoon Jeong ) 대한원격탐사학회 2024 대한원격탐사학회지 Vol.40 No.5

The Geostationary Environment Monitoring Spectrometer (GEMS) onboard the Geostationary Korea Multi-Purpose Satellite-2B (GEO-KOMPSAT-2B) satellite, launched in February 2020, represents a pioneering milestone in air quality monitoring across East and Southeast Asia. GEMS provides hourly data on atmospheric pollutants, including nitrogen dioxide (NO₂), sulfur dioxide (SO₂), ozone (O₃), volatile organic compounds such as formaldehyde (HCHO) and glyoxal (CHOCHO), as well as aerosols, all with high spatial resolution. The Environmental Satellite Center (ESC) of the National Institute of Environmental Research (NIER) is responsible for processing, retrieving, and distributing GEMS data, offering critical insights into the transport and spatial distribution of these pollutants. GEMS data has been instrumental in analyzing significant air pollution events, such as episodes of elevated particulate matter, wildfires, and volcanic eruptions. Additionally, ongoing research projects led by ESC are focused on developing novel application techniques, including satellite data fusion, top-down emissions estimation, and nighttime pollutant detection. GEMS operates as part of a global geostationary constellation, alongside the United States’ Tropospheric Emissions: Monitoring of Pollution (TEMPO) and Europe’s Sentinel-4, enhancing both the spatial and temporal coverage of air pollutants and facilitating data sharing for quality assurance. Looking ahead, ESC aims to expand its environmental monitoring capabilities by launching a constellation of microsatellites dedicated to greenhouse gas monitoring, together with the next generation of GEMS, which will continue its air quality monitoring missions. This paper presents an overview of GEMS operations, data products, and applications while outlining future strategies for enhancing air quality monitoring and supporting environmental policies aimed at clean air and climate mitigation.

<i>Rhodanobacter koreensis</i> sp. nov., a bacterium isolated from tomato rhizosphere

Won, KyungHwa,Singh, Hina,Ngo, Hien T. T.,Son, HeungMin,Kook, MooChang,Kim, Ki–,Young,Yi, Tae–,Hoo International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.4

<P>A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7<SUP>T</SUP>, was isolated from tomato plant rhizosphere soil. Strain THG-DD7<SUP>T</SUP> grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 0.5 % (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7<SUP>T</SUP> was most closely related to <I>Rhodanobacter umsongensis</I> GR24-2<SUP>T</SUP> (98.2 %), <I>Rhodanobacter panaciterrae</I> LnR5-47<SUP>T</SUP> (98.0 %), <I>Rhodanobacter soli</I> DCY45<SUP>T</SUP> (97.9 %), <I>Rhodanobacter terrae</I> GP18-1<SUP>T</SUP> (97.9 %) and <I>Dyella ginsengisoli</I> Gsoil 3046<SUP>T</SUP> (97.7 %). The DNA G+C content was 65.2 mol%. In DNA–DNA hybridization, the DNA relatedness levels between strain THG-DD7<SUP>T</SUP> and its closest phylogenetically neighbours were below 40.0 %. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-<I>N</I>-methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, anteiso-C<SUB>15 : 0</SUB> and iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and/or C<SUB>16 : 0</SUB> 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7<SUP>T</SUP> to the genus <I>Rhodanobacter</I> . The results of physiological and biochemical tests enabled strain THG-DD7<SUP>T</SUP> to be differentiated genotypically and phenotypically from the species of the genus <I>Rhodanobacter</I> with validly published names. Therefore, the novel isolate represents a novel species, for which the name <I>Rhodanobacter koreensis</I> sp. nov. is proposed. The type strain is THG-DD7<SUP>T</SUP> ( = KACC 17650<SUP>T</SUP> = JCM 19614<SUP>T</SUP>).</P>

Phycicoccus soli sp. nov., isolated from soil.

Singh, Hina,Won, KyungHwa,Ngo, Hien T T,Du, Juan,Kook, MooChang,Yi, Tae-Hoo Society for General Microbiology 2015 International journal of systematic and evolutiona Vol.65 No.8

<P>A Gram-stain-positive, non-motile, coccus-shaped bacterium, strain THG-a14T, was isolated from soil of Gyeyang mountain in Incheon, Republic of Korea. The isolate grew optimally at 28?C, at pH?6.5-7.5 and with 0-3?% (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-a14T was closely related to Phycicoccus aerophilus 5516T-20T (97.7?%), P. ginsenosidimutans BXN5-13T (97.6?%), 'P. ochangensis' L1b-b9 (97.4?%) and P. bigeumensis MSL-03 (97.2?%). The DNA G+C content of strain THG-a14T was 71.6?mol%. In DNA-DNA hybridization, the DNA-DNA relatedness between strain THG-a14T and its closest phylogenetically neighbours was below 50.0?%. Strain THG-a14T was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan. Strain THG-a14T contained glucose and ribose as whole-cell-wall sugars and menaquinone MK-8(H4) as the major isoprenoid quinone. Polar lipids in strain THG-a14T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoaminoglycolipids, unidentified phospholipids and unidentified lipids. The major fatty acids were iso-C16:0, iso-C15:0 and C17:1ω8c. On the basis of our polyphasic taxonomy study, strain THG-a14T represents a novel species within the genus Phycicoccus, for which the name Phycicoccussoli sp. nov. is proposed. The type strain is THG-a14T (?=?KACC 17892T?=?JCM 19837T).</P>

Response to “Medical Statistics Unlock the Gateway to Further Research: Using Deep Learning to Predict CDKN2A/B Homozygous Deletion in Isocitrate Dehydrogenase-Mutant Astrocytoma”

Park Yae Won,Han Kyunghwa,Kim Se Hoon 대한영상의학회 2023 Korean Journal of Radiology Vol.24 No.12

Lysobacter agri sp. nov., a bacterium isolated from soil

Singh, Hina,Won, KyungHwa,Du, Juan,Yang, Jung-Eun,Akter, Shahina,Kim, Ki-Young,Yi, Tae-Hoo Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3

<P>A bacterial strain, designated as THG-SKA3(T), was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28?C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3(T) shared highest sequence similarity with Lysobacter niastensis KACC 11588(T) followed by Lysobacter panacisoli KACC 17502(T), Lysobacter enzymogenes LMG 8762(T) and Lysobacter oryzae KCTC 22249(T). The G+C content of THG-SKA3(T) was determined to be 68.9?mol%. The DNA-DNA relatedness values between strain THG-SKA3(T) and its closest phylogenetic neighbors were below 25.0?%.The major polar lipids of strain THG-SKA3(T) were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C15:0, iso-C16:0 and unsaturated iso-C17:1 ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3(T)?=?KACC 18283(T)?=?CSCTCC AB 2015126(T)) is proposed.</P>

The occurrence and distribution of polychlorinated naphthalenes (PCNs), focusing on tissue-specific bioaccumulation in crucian carp in South Korea

Kim, Kun-Won,Choo, Gyojin,Cho, Hyeon-Seo,Lee, Byoung-Cheun,Park, Kyunghwa,Oh, Jeong-Eun Elsevier 2019 The Science of the total environment Vol.665 No.-

<P><B>Abstract</B></P> <P>The occurrence and distribution of 41 congeners of polychlorinated naphthalenes (PCNs) were investigated in major tissues of crucian carp (muscle, gonad, liver, and blood) and in river sediment to assess the bioaccumulation potential of PCNs. The total PCN concentrations in sediment ranged from 4.37 to 19.5 pg/g dry weight (dw), mainly comprising CN-13, CN-33/34/37, and CN-38/40. Among fish tissues, higher PCN concentrations were observed in the gonads (29.6 ± 10.3 pg/g wet weight, ww) and liver (25.7 ± 4.35 pg/g ww) of crucian carp than in the muscle (2.17 ± 0.68 pg/g ww). CN-28/43, CN-33/34/37, CN-42, CN-52/60, CN-53/55, and CN-66/67 congeners were detected most frequently and had the highest concentration among all crucian carp tissues. We investigated the association between the PCN concentration in each tissue (muscle, liver, and gonad) and the size of the fish (total length and weight) and found positive correlations between the PCN concentration in the gonads and the total length and weight (<I>p</I> < 0.05, Spearman correlation).</P> <P><B>Highlights</B></P> <P> <UL> <LI> The level and distribution of PCNs were investigated in sediment and fish. </LI> <LI> The PCNs accumulation in sediment was mainly influenced by combustion and Halowax. </LI> <LI> The congeners with PCN-NVC and PCN-DVC structures had high levels in fish tissues. </LI> <LI> PCNs levels in gonad tissue of crucian carp increased as crucian carp grew. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Sphingomonas flavus sp. nov. isolated from road soil

Du, Juan,Singh, Hina,Won, KyungHwa,Yang, Jung-Eun,Akter, Shahina,Jin, Feng-Xie,Yi, Tae-Hoo Springer-Verlag 2015 Archives of microbiology Vol.197 No.7

<P>A yellow-colored, Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-MM5(T), was isolated from road soil in Yongin-si, Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence, strain THG-MM5(T) was moderately related to Sphingomonas sediminicola KACC 15039(T) (96.1?%), Sphingomonas ginsengisoli KACC 16858(T) (96.1?%) and Sphingomonas jaspsi KACC 13230(T) (96.0?%). Chemotaxonomic data revealed that strain THG-MM5(T) possesses ubiquinone-10 as the only respiratory quinone, sym-homospermidine as the major polyamine and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω7c and C16:0 as the major fatty acids. The polar lipid profile included sphingoglycolipid. The DNA G?+?C content was 60.7?mol%. These data, together with phenotypic characterization, corroborated the affiliation of strain THG-MM5(T) to the genus Sphingomonas. Thus, the isolate represents a novel species, for which the name Sphingomonas flavus sp. nov. is proposed, with THG-MM5(T) as the type strain (=KACC 18277(T)?=?CCTCC AB 2014320(T)).</P>

Taibaiella yonginensis sp. nov., a bacterium isolated from soil of Yongin city.

Singh, Hina,Du, Juan,Won, KyungHwa,Yang, Jung-Eun,Akter, Shahina,Kim, Ki-Young,Yin, ChangShik,Yi, Tae-Hoo N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.108 No.3

<P>A novel bacterial strain, designated as THG-SC4(T), was isolated from a soil sample collected from Yongin city in South Korea. Cells of the strain were Gram-negative, aerobic, rod-shaped and non-motile. The strain grew optimally at 28-30?C; at pH 7.0 and in the absence of NaCl. Flexirubin-type pigments were found to be present. On the basis of 16S rRNA gene sequence similarity, strain THG-SC4(T) was shown to belong to the genus Taibaiella and shares high sequence similarity with Taibaiella smilacinae KCTC 32316(T) (95.4?%), followed by Taibaiella koreensis KACC 17171(T) (94.3?%) and Taibaiella chishuiensis JCM 19637(T) (94.2?%). The DNA G+C content of the novel isolate was determined to be 43.1?mol% and MK-7 was identified as the predominant isoprenoid quinone. The only polyamine was homospermidine. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified polar lipid. The predominant fatty acids were identified as iso-C15:0, iso-C15:1 G, C16:0 and iso-C17:03-OH. On the basis of data from this polyphasic taxonomic study, strain THG-SC4(T) is considered to represent a novel species of the genus Taibaiella, for which the name Taibaiella yonginensis sp. nov. is proposed. The type strain is THG-SC4(T) (=KACC 18372(T) = CCTCC AB 2014316(T)).</P>

Sphingosinicella cucumeris sp. nov., isolated from soil of a cucumber garden.

Akter, Shahina,Du, Juan,Won, KyungHwa,Yin, Chang Shik,Kook, MooChang,Yi, Tae-Hoo N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.108 No.5

<P>A novel bacterial strain, THG-sc1(T), was isolated from a soil sample of a cucumber garden and was characterised by using a polyphasic approach. Cells were observed to be Gram-stain negative, non-motile and rod-shaped. The strain was found to be aerobic, catalase and oxidase positive, esculin and starch negative, and to have an optimum growth temperature and pH of 28??°C and 7.5, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-sc1(T) was found to belong to the genus Sphingosinicella and to be closely related to Sphingosinicella vermicomposti KCTC 22446(T), followed by Sphingosinicella xenopeptidilytica DSM 17130(T) and Sphingosinicella microcystinivorans KCTC 12019(T). The DNA G+C content was determined to be 60.8??mol% and the predominant respiratory quinone was identified as ubiquinone-10. The major polyamine was found to be sym-homospermidine. The major polar lipids were identified as sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The major fatty acids were identified as C18:1 ??7c, C16:0 and summed feature 3 (C16:1 ??7c and/or iso-C15:0 2-OH, as defined by MIDI). The results of the genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain THG-sc1(T) represents a novel species within the genus Sphingosinicella, for which the name Sphingosinicella cucumeris is proposed. The type strain is THG-sc1(T) (=KACC 18279(T)??=??CCTCC AB 2015120(T)).</P>