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Liao Guangrong,Li Jitong,Fan Wenyan,Chen Lin,Liu Jinglan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
The incidence of drought stress in plants has been increasing due to global warming, and the phytohormone abscisic acid (ABA) induces the formation of physical barriers in plants, such as callose accumulation. The brown planthopper (BPH; Nilaparvata lugens Stål) occurs throughout Asia and feeds on rice, but the effects of drought stress on BPH feeding remain unclear. In this study, we observed changes in callose formation and ABA content in rice during drought stress. ABA content and the relative expression of ABA synthesis genes OsNCED3 and OsNCED5 were higher in drought-stressed rice than the non-stressed control. Similarly, the expression levels of callose synthesis genes and callose deposition were significantly higher in drought-stressed rice as compared to non-stressed plants, and this impacted BPH feeding. Our results indicated that rice resistance to BPH increased during drought stress due to the accumulation of callose and increasing ABA levels. Our findings provide a basis for understanding BPH feeding performance on rice during drought stress and offer novel insights relative to control during periods of water shortage.
Identification and Characterization of a Novel Antibacterial Peptide, Avian β-Defensin 2 from Ducks
Deying Ma,Ruiqin Wang,Wenyan Liao,Zongxi Han,Shengwang Liu 한국미생물학회 2009 The journal of microbiology Vol.47 No.5
In this study, a novel avian β-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20°C to 100°C) and pH values (range, 3 to 12).