Taxonomy of the genus Laena from Yunnan in China, with description of eight new species (Coleoptera: Tenebrionidae: Lagriinae)

Wei Zhonghua,Zhao Xiaolin,Ren Guodong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Eight new species of the genus Laena Dejean, 1821 (subfamily Lagriinae, tribe Laenini) from Yunnan Province of China were described and illustrated: L. acutidentata Wei & Ren, sp. nov., L. brevicarina Wei & Ren, sp. nov., L. dongchuana Zhao & Ren, sp. nov., L. glabridentipa Wei & Ren, sp. nov., L. nuda Zhao & Ren, sp. nov., L. raropuncta Wei & Ren, sp. nov., L. rugulosa Wei & Ren, sp. nov. and L. spinicla Wei & Ren, sp. nov.. Illus trations and a key to the known Laena species from Yunnan Province are provided.

Taxonomic study of the genus Laena from Sichuan in China, with description of four new species (Coleoptera: Tenebrionidae)

Wei Zhonghua,Zhao Xiaolin,Ren Guodong 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

The genus Laena Dejean, 1821 belongs to the subfamily Lagriinae, tribe Laenini. Four new species of this genus are described and illustrated from Sichuan under the following name: Laena ceratina Wei & Ren, sp. nov., Laena edentata Zhao & Ren, sp. nov. (from Sichuan and Qinghai), Laena latitarsia Wei & Ren, sp. nov. (from Sichuan and Yunnan) and Laena sufflofemora Wei & Ren, sp. nov.. A key to the known Laena species from Sichuan is provided.

Two new species of the genus Endelus Deyrolle, 1864 from China (Coleoptera: Buprestidae)

Wei Zhonghua,Shi Aimin 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

The genus Endelus Deyrolle, 1864 belongs to the subfamily Agrilinae, tribe Aphanisticini. Two new species, E. (E.) violaceithorax sp. n. from Hainan Island and E. (E.) wangi sp. n. from Guangxi and Yunnan, are herein described and illustrated. A key to separate species of the subgenus Endelus Deyrolle, 1864 from China is provided.

Pricing Strategies of Social Commerce Platform under Network Externalities

Wei Zhang,Wei Ma,Zhonghua Li,Naixue Xiong 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.12

Although the theory of two-sided markets is popular nowadays, little attention was paid on self-network externalities and UGC (User Generated Content) produced by buyers which are the major features of social commerce two-sided markets. This article studies percentages of buyers who generated UGC, self-network externalities of buyers and cross-group externalities between two groups. The three factors influence the pricing strategy of social commerce platforms based on two-sided markets. With analyzing the special characteristics of social commerce, the study unveils that the optimal price of buyers charged by social commerce platform shows a negative correlation with self-network externalities and it also shows a negative correlation with the probability of UGC generated by buyers. The study also shows that the equilibrium price of buyers declines with the augment of cross-group externalities of buyers to sellers, and the equilibrium price of sellers declines with the augment of cross-group work externalities of sellers to buyers. The social commerce platforms on the basis of interest graph, lower UGC threshold and more interactive among customers gain the competitive advantages. Compared to other platforms, the two-sided social commerce platforms give consumers and retailers more subsidies.

The genus Anaedus Blanchard, 1842 in China with description of two new species (Coleoptera: Tenebrionidae: Goniaderini)

Zhonghua Wei,Guodong Ren 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

The genus Anaedus Blanchard, 1842 from China is revised. Anaedus serratus sp. nov. (Yunnan) and Anaedus nonedgus sp. nov. (Anhui, Hubei, Zhejiang) are described and illustrated. In addition to, a key to Chinese Anaedus species is given, and their geographical locations have been mapped.

Mapping the Mangrove Forest Restoration Potential and Conservation Gaps in China Based on Random Forest Model

Zhonghua Yu,Wei Li,Shaowei Zhang,Buqing Zhong,Jun Wang,이시영,최재혁,Shulin Deng 인간식물환경학회 2022 인간식물환경학회지 Vol.25 No.4

Background and objective: The area of mangroves is gradually decreasing globally, and mangroves are already one of the most threatened ecosystems. Despite net growth in the mangrove areas in China, the restoration potential of mangroves is still insufficient. This study proposed the Random forest model as an excellent data mining method to map the restoration potential based on the predicted probability of mangrove habitat suitability. Methods: We demonstrated the vital environmental variables influencing habitat suitability. The de-cisive advantages of RFM were parsimonious (variables selection), cost-effective (us-ing existing open-source data), accurate (training AUC was 0.89, testing AUC was 0.91), highly efficient (fast-training speed); and its results had high explanatory power. Here, we first mapped the conservation gaps using the RFM. Results: The results showed that temperature was the most important environmental factor influencing the habitat suit-ability of mangroves. The northern limit of suitable areas was around 24°44' N. The theoretical suitable habitat area for mangrove was 196,566.6 ha (the highly suitable area was 32,551.4 ha, the medium suitable area was 164,015.2 ha). The potential area for mangrove restoration was 176,264 ha (Guangdong with 104215.4 ha, Guangxi with 65957.5 ha). Conclusion: We proposed 24 sites with conservation gaps for mangrove forests restoration and nine potential sites as examples for the further restoration plan. We took one example site with high restoration potential for further explanation: how the key environmental factors influence the habitat suitability and how to use the infor-mation to guide the restoration strategies. RFM can be used as a data mining algo-rithm for the utmost use of the presence-only ecological data, objectively evaluating the suitability of species distribution, and providing scientifically technical data for species restoration planning.

Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system

Renyue Wei,Jiawei Lv,Xuechun Li,Yan Li,Qianqian Xu,Junxue Jin,Yu Zhang,Zhonghua Liu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

The genome of the cucumber, Cucumis sativus L.

Huang, Sanwen,Li, Ruiqiang,Zhang, Zhonghua,Li, Li,Gu, Xingfang,Fan, Wei,Lucas, William J,Wang, Xiaowu,Xie, Bingyan,Ni, Peixiang,Ren, Yuanyuan,Zhu, Hongmei,Li, Jun,Lin, Kui,Jin, Weiwei,Fei, Zhangjun,Li Nature Publishing Group 2009 Nature genetics Vol.41 No.12

Cucumber is an economically important crop as well as a model system for sex determination studies and plant vascular biology. Here we report the draft genome sequence of Cucumis sativus var. sativus L., assembled using a novel combination of traditional Sanger and next-generation Illumina GA sequencing technologies to obtain 72.2-fold genome coverage. The absence of recent whole-genome duplication, along with the presence of few tandem duplications, explains the small number of genes in the cucumber. Our study establishes that five of the cucumber's seven chromosomes arose from fusions of ten ancestral chromosomes after divergence from Cucumis melo. The sequenced cucumber genome affords insight into traits such as its sex expression, disease resistance, biosynthesis of cucurbitacin and 'fresh green' odor. We also identify 686 gene clusters related to phloem function. The cucumber genome provides a valuable resource for developing elite cultivars and for studying the evolution and function of the plant vascular system.

The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells

Jiawei Lv,Shuang Wu,Renyue Wei,Yan Li,Junxue Jin,Yanshuang Mu,Yu Zhang,Qingran Kong,Xiaogang Weng,Zhonghua Liu 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.3

The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly used in application of CRISPR/Cas9; however, the relationship between the length of the guide sequence and the efficiency of CRISPR/ Cas9 in porcine cells is still not clear. To illustrate this issue, guide RNAs of different lengths targeting the EGFP gene were designed. Specifically, guide RNAs of 17 nt or longer were sufficient to direct the Cas9 protein to cleave target DNA sequences, while 15 nt or shorter guide RNAs had loss-of-function. Full-length guide RNAs complemented with mismatches also showed loss-of-function. When the shortened guide RNA and target DNA heteroduplex (gRNA:DNA heteroduplex) was blocked by mismatch, the CRISPR/Cas9 would be interfered with. These results suggested the length of the gRNA:DNA heteroduplex was a key factor for maintaining high efficiency of the CRISPR/Cas9 system rather than weak bonding between shortened guide RNA and Cas9 in porcine cells.