5-Formylhonokiol exerts anti-angiogenesis activity via inactivating the ERK signaling pathway

Wei Zhu,Lijuan Chen,Afu Fu,Jia Hu,Tianen Wang,Youfu Luo,Ming Peng,Yinghua Ma,Yuquan Wei 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.3

Our previous report has demonstrated that 5-formylhonokiol (FH), a derivative of honokiol (HK), exerts more potent anti-proliferative activities than honokiol in several tumor cell lines. In present study, we first explored the antiangiogenic activities of 5-formylhonokiol on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) for the first time in vitro. Then we investigated the in vivo antiangiogenic effect of 5-formylhonokiol on zebrafish angiogenesis model. In order to clarify the underlying molecular mechanism of 5-formylhonokiol, we investigated the signaling pathway involved in controlling the angiogenesis process by western blotting assay. Wound-healing results showed that 5-formylhonokiol significantly and dose-dependently inhibited migration of cultured human umbilical vein enthothelial cells. The invasiveness of HUVEC cells was also effectively suppressed at a low concentration of 5-formylhonokiol in the transwell assay. Further F-actin imaging revealed that inhibitory effect of 5-formylhonokiol on invasion may partly contribute to the disruption of assembling stress fiber. Tube formation assay, which is associated with endothelial cells migration,further confirmed the anti-angiogenesis effect of 5-formylhonokiol. In in vivo zebrafish angiogenesis model, we found that 5-formylhonokiol dose-dependently inhibited angiogenesis. Furthermore, western blotting showed that 5-formylhonokiol significantly down-regulated extracellular signal-regulated kinase (ERK) expression and inhibited the phosphorylation of ERK but not affecting the total protein kinase B (Akt)expression and related phosphorylation, suggesting that 5-formylhonokiol might exert anti-angiogenesis capacity via down-regulation of the ERK signal pathway. Taken together, these data suggested that 5-formylhonokiol might be a viable drug candidate in antiangiogenesis and anticancer therapies.

Characterization and evolutionary analysis of Brassica species-diverged sequences containing simple repeat units

Lijuan Wei,Meili Xiao,Annaliese S. Mason,Bi Ma,Kun Lu,Jia Na Li,Link Katrin,Donghui Fu 한국유전학회 2013 Genes & Genomics Vol.35 No.2

Brassica species, B. napus (canola), B. rapa and B. oleracea, are important sources of nutritionally valuable vegetable oil and protein-rich meal for animals and humans. Sequencing of the model plant Arabidopsis thaliana, has opened the way for investigations into the complex structure of the Brassica genomes, offering important insight into their evolution and composition. We use this sequence information for the characterization and functional analysis of SSR sequences that have diverged between the Brassica species. A total of 56 species-diverged sequences containing simple repeat units (SDS-SSR) of B. napus and its diploid progenitor species B. rapa and B. oleracea were isolated and characterized. Of these, 40 sequences showed homology with other Brassica sequences. Using the SSR Locator software, only 23 sequences were found to have SSRs,possibly due to the loss of SSR units in the process of species divergence. Sequence alignments with A. thaliana revealed that these species-diverged SSR sequences were responsible for Brassica divergence for differences between Brassica species in several genomic regions. Six active genes related to transferase, protein, transcription factor and retroelements were found in the SDS-SSRs. These results will further improve our understanding of the characteristics of species-diverged SSR fragments and their contribution to genome differentiation.

5-Formylhonokiol exerts anti-angiogenesis activity $via$ inactivating the ERK signaling pathway

Zhu, Wei,Fu, Afu,Hu, Jia,Wang, Tianen,Luo, Youfu,Peng, Ming,Ma, Yinghua,Wei, Yuquan,Chen, Lijuan Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.3

Our previous report has demonstrated that 5-formylhonokiol (FH), a derivative of honokiol (HK), exerts more potent anti-proliferative activities than honokiol in several tumor cell lines. In present study, we first explored the antiangiogenic activities of 5-formylhonokiol on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) for the first time $in$ $vitro$. Then we investigated the $in$ $vivo$ antiangiogenic effect of 5-formylhonokiol on zebrafish angiogenesis model. In order to clarify the underlying molecular mechanism of 5-formylhonokiol, we investigated the signaling pathway involved in controlling the angiogenesis process by western blotting assay. Wound-healing results showed that 5-formylhonokiol significantly and dose-dependently inhibited migration of cultured human umbilical vein enthothelial cells. The invasiveness of HUVEC cells was also effectively suppressed at a low concentration of 5-formylhonokiol in the transwell assay. Further F-actin imaging revealed that inhibitory effect of 5-formylhonokiol on invasion may partly contribute to the disruption of assembling stress fiber. Tube formation assay, which is associated with endothelial cells migration, further confirmed the anti-angiogenesis effect of 5-formylhonokiol. In $in$ $vivo$ zebrafish angiogenesis model, we found that 5-formylhonokiol dose-dependently inhibited angiogenesis. Furthermore, western blotting showed that 5-formylhonokiol significantly down-regulated extracellular signal-regulated kinase (ERK) expression and inhibited the phosphorylation of ERK but not affecting the total protein kinase B (Akt) expression and related phosphorylation, suggesting that 5-formylhonokiol might exert anti-angiogenesis capacity $via$ down-regulation of the ERK signal pathway. Taken together, these data suggested that 5-formylhonokiol might be a viable drug candidate in antiangiogenesis and anticancer therapies.

Novel Flower-Like CuO/N-rGO as Enhanced Electrocatalyst for Oxygen Reduction Reaction

Lijuan Zhang,Jinhua Lu,Jianfeng Wei,Yan Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.10

Flower-like copper oxide (CuO)/nitrogen-doped reduced graphene oxide (N-rGO) was synthesized through a one-pot microwave hydrothermal method by using polyvinylpyrrolidone (PVP) as surfactant. In the process, in situ formation of nanomaterial CuO, reduction of graphene oxide and doping of nitrogen species occurred simultaneously in urea solution. The structural and surface properties of the material were investigated by field emission scanning electron microscopy (FESEM) and transmission electron microscopies (TEM), the energy dispersive spectroscopic (EDS) and powder X-ray diffraction (XRD). This showed the flower-like CuO with an interconnected architecture was successfully uniformed and grown on the surface of N-rGO. Moreover, the surfactant PVP and urea were found to be the key factors to control the morphology of the CuO nanostructure. Electrochemical investigations indicated that the CuO/N-rGO composite exhibited a significantly enhanced ORR activity in comparison to pure CuO and N-rGO in an alkaline solution. The enhancement in ORR activity of CuO/N-rGO composite can be attributed to the synergistic effects of good electron transport from N-rGO as well as abundance of exposed catalytic sites and meso/macroporosity from CuO nanostructures.

Dermoscopy for the Diagnosis of Creeping Hair Caused by Ingrowing Hair: A Case Report

Lijuan Xu,Xinhai Liu,Wei Zhu,Lingling Zheng 대한피부과학회 2023 Annals of Dermatology Vol.35 No.-

Creeping hair is a rare condition characterized by creeping eruption with a black line at the advancing end, mimicking cutaneous larva migrans. The condition is also referred to as cuta- neous pili migrans, migrating hair, and embedded hair. A total of 52 cases have been reported since 1957 and most cases were published in English. Herein, we report a case in which creep- ing hair occurred in the iliac region and review the literature from 1957 to February 2021. A 35-year-old Chinese female presented with a black moving linear eruption that had migrated from the lower abdomen to the iliac region without causing any symptoms during a 3-year period. Cutaneous examination showed a 6.5-cm-long black linear lesion beneath the skin that was revealed to be a hair shaft. After removal of the hair, the eruption diminished and no re- currence occurred in 3 months of follow-up. The creeping hair that had migrated with its lower end forward was confirmed by observation under dematoscopy and light microscopy. A review of the literature revealed that creeping hair occurs most frequently in young and middle-aged patients and the reported cases are mainly from Asia. The top locations involve the foot. The causative hair includes head hair, beard, pubic hair, body hair, and one case of dog hair. A close- up examination and dermoscopic inspection are helpful for the diagnosis of creeping hair.

One-Step Solvothermal Synthesis of Tetranitro-Cobalt Phthalocyanine/Reduced Graphene Oxide Composite

Lijuan Zhang,Hejun Li,QIANGANG FU,Zhanwei Xu,Kezhi Li,Jianfeng Wei 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.3

Tetranitro-cobalt phthalocyanine/reduced graphene oxide (TNCoPc/RGO) composite was prepared using ethylene glycol as reducing agent and precursor. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR) were used to characterize the composite. The electrochemical performance of the composite was examined by cyclic voltammetry and rotating disk electrode. The SEM observation and UV-Vis absorption spectrophotometer reveal that TNCoPc nanoparticles are closely contacted with RGO. The results show that the composite has a larger peak current and a higher current density than TNCoPc for oxygen reduction in an alkaline medium. This work offers a simple and environmental route for the synthesis of metal phthalocyanine (MPc)/RGO, which is adaptable to a wide range of applications in fuel cells.

ON POLARS OF MIXED COMPLEX PROJECTION BODIES

Lijuan Liu,Wei Wang,Qingzhong Huang 대한수학회 2015 대한수학회보 Vol.52 No.2

In this paper we establish general Minkowski inequality, Aleksandrov-Fenchel inequality and Brunn-Minkowski inequality for polars of mixed complex projection bodies.

FOURIER TRANSFORM AND Lp-MIXED PROJECTION BODIES

Lijuan Liu,Wei Wang,Binwu He 대한수학회 2010 대한수학회보 Vol.47 No.5

In this paper we define the Lp-mixed curvature function of a convex body. We develop a formula connection the support function of Lp-mixed projection body with Fourier transform of the Lp-mixed curvature function. Using this formula we solve an analog of the Shephard projection problem for Lp-mixed projection bodies.

An Analysis of the "Blank Space" in Freehand Flower and Bird Painting from an Aesthetic Perspective

Lijuan Liu,Yue Zhang,Jie Meng,Xiaobo Lian,Jun Wei,Jingyuan Wang KOREA INSTITUTE FOR HUMANITIES AND SOCIAL SCIENCES 2023 Journal of Arts and Cultural Studies(ACS) Vol.2 No.2

Background: "Leave blank" is a unique expression technique in Chinese painting. It has the unique charm of Chinese national culture. This article analyzes the artistic "Blank Space" expression in freehand brushwork flower and bird paintings from an aesthetic perspective. In aesthetics field, artistic beauty is an essential category. It can also be seen as a study of the aesthetic relationship between humans and the objective world. The "Blank Space" in Chinese freehand flower and bird paintings is important in ancient Chinese art. Purpose: The word "Blank Space" is easily understood and is a commonly used expression technique in Chinese art. It has strong Chinese aesthetic characteristics. This article analyzes the connotation and significance of "Blank Space". It studies freehand brushwork, composition, expression, and formal beauty of “Blank Space”. It elaborates the aesthetic expression of “Blank Space” in freehand flower and bird paintings. Furthermore, it has created various artistic beauty that make people feel graceful, melodious, and melancholic, with endless aftertaste. Methods: Based on the literature, analyze the art of leaving white space in the painting from an aesthetic perspective. Understand and master the importance of leaving white space in freehand flower and bird paintings comprehensively and correctly. Results: The application of "Blank Space" in the painting reflects the aesthetic taste of Chinese painting. It is the unique charm of traditional Chinese art. The "Blank Space" artistic techniques enriches and develops the artistic style of Chinese freehand flower and bird painting. Conclusion: The artistic conception, implicit beauty, spatial sense, and rhythmic rhythm of the screen perfectly express the "blank" art of Chinese freehand brushwork of flowers and birds. Blank space art uses "Blank Space" as a carrier to render beautiful artistic conceptions with strong Chinese aesthetic characteristics. Most works combine virtual and real, bringing readers a higher aesthetic enjoyment. It expands the hierarchical layout of the space, leaving room for imagination.

FOURIER TRANSFORM AND L_p-MIXED PROJECTION BODIES

Liu, Lijuan,Wang, Wei,He, Binwu Korean Mathematical Society 2010 대한수학회보 Vol.47 No.5

In this paper we define the $L_p$-mixed curvature function of a convex body. We develop a formula connection the support function of $L_p$-mixed projection body with Fourier transform of the $L_p$-mixed curvature function. Using this formula we solve an analog of the Shephard projection problem for $L_p$-mixed projection bodies.