Three-Port Laparoscopic Exploration is not Sufficient for Patients with T4 Gastric Cancer

Huang, Hua,Jin, Jie-Jie,Long, Zi-Wen,Wang, Wei,Cai, Hong,Liu, Xiao-Wen,Yu, Hong-Mei,Zhang, Li-Wen,Wang, Ya-Nong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Gastric cancer continues to be a leading cause of cancer death. The majority of patients with gastric adenocarcinoma in China present with advanced disease. Ruling out unresectable cancers from an unnecessary "open" exploration is very important. The aim of this study was to assess the value of five-port anatomical laparoscopic exploration in T4 gastric cancer in comparison with three-port laparoscopic exploration and laparotomy exploration. We conducted a retrospective study on 126 patients with T4 stage scheduled for D2 curative gastrectomy based on computed tomography (CT) staging at Department of Gastric Cancer and Soft Tissue Sarcoma, Fudan University Shanghai Cancer Center, from Apr. 2011 to Apr. 2013. Laparotomy exploration (Group I), three-port laparoscopic exploration (Group II) or five-port anatomical laparoscopic exploration (Group III) were performed prior to radical gastrectomy. Accuracy rate for feasibility of D2 curative gastrectomy in laparotomy exploration and five-port anatomical laparoscopic exploration groups was higher than that in the three-port laparoscopic exploration group. Five-port anatomical laparoscopic exploration group had the highest accuracy resection rate (Group I vs Group II vs Group III,92.6% vs78.6% vs 97.7%; p<0.05) and shorter length of hospitalization (Group I vs Group II vs Group III, $9.58{\pm}4.17$ vs $6.13{\pm}2.85$ vs $5.00{\pm}1.81$; p<0.001). Three-port laparoscopic exploration has low accuracy rate for assessing feasibility of D2 curative gastrectomy and five-port anatomical laparoscopic exploration should be performed on patients with T4 gastric cancer.

Robust State Observer Design for Dynamic Connection Relationships in Complex Dynamical Networks

Zi-lin Gao,Yin-He Wang,Jiang Xiong,Li-li Zhang,Wen-li Wang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.2

The complex dynamical network with the time-varying links may be regarded to be composed of the twomutually coupled subsystems, which are called the nodes subsystem and the connection relationships subsystemrespectively. Designing the state observer for the nodes subsystem has been discussed in some existing researches byemploying the known connection relationships. However, the state observer design for the connection relationshipssubsystem is not shown in the existing researches. In practical applications, the connection relationships subsystempossesses also the state variables such as the relationship strength in social network, the size of web tension in theweb winding system. Therefore, designing the state observer for the connection relationships subsystem is also ofpractical significance. In this paper, a novel state observer is proposed for the connection relationships subsystemmodeled mathematically by the Riccati matrix differential equation. The observer utilizes the output measurementsof the connection relationships subsystem and the state of nodes subsystem. Compared with the state observerof nodes subsystems which employs the known connection relationships, the state observer for the connectionrelationships subsystem employs the state of nodes subsystem and is represented in form of the matrix differentialequation. By using Lyapunov stability theory, it is proved in this paper that the state observer for the connectionrelationships subsystem is asymptotical under certain mathematic conditions. Finally, the illustrative simulation isgiven to show the efficiency and validity of the proposed method in this paper.

A unique genetic lineage at the southern coast of China in the agar-producing Gracilaria vermiculophylla(Gracilariales, Florideophyceae)

Zi-Min Hu,Ruo-Yu Liu,Jie Zhang,De-Lin Duan,Gao-Ge Wang,Wen-Hong Li 한국조류학회I 2018 ALGAE Vol.33 No.3

Ocean warming can have significant negative impacts on population genetic diversity, local endemism and geographical distribution of a wide range of marine organisms. Thus, the identification of conservation units with high risk of extinction becomes an imperative task to assess, monitor, and manage marine biodiversity for policy-makers. Here, we surveyed population structure and genetic variation of the red seaweed Gracilaria vermiculophylla along the coast of China using genome-based amplified fragment length polymorphism (AFLP) scanning. Regardless of analysis methods used, AFLP consistently revealed a south to north genetic isolation. Populations at the southern coast of China showed unique genetic variation and much greater allelic richness, heterozygosity, and average genetic diversity than the northern. In particular, we identified a geographical barrier that may hinder genetic exchange between the two lineages. Consequently, the characterized genetic lineage at the southern coast of China likely resulted from the interplay of post-glacial persistence of ancestral diversity, geographical isolation and local adaptation. In particular, the southern populations are indispensable components to explore evolutionary genetics and historical biogeography of G. vermiculophylla in the northwestern Pacific, and the unique diversity also has important conservation value in terms of projected climate warming.

Establishment and Analysis of Erosion Depth Model for Impeller Material FV520B

Zi-Wu Liu,Jian-Feng Li,Xiu-Jie Jia,Guang-Cun Wang,Wen-Han Xu 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.3 No.1

The multifactorial erosion was conducted in this paper to test the compressor impeller material FV520B using high-speed gas-solid two phase flow erosion tester and surface morphology analysis method. Based on the particle motion and collision energy equation as well as regression analysis of multi-factor orthogonal experiment, a phenomenological erosion depth model which captures the effects of impact velocity, angle and particle size, has been developed. The model includes removal of material due to both deformation damage and micro-cutting. Results show that the peak of experiment depth and the maximum calculated depth all appeared at near 45o, rather than near 24o where the maximum erosion rate appeared. Comparing the calculated values and the results of each single factor experiment, the errors are within 15%. The predictions of the simplified version of the model were in good agreement with the results of single factor experiments. Also, the reliability of the assessment formula was verified to assess the impeller erosion life, which indicated that this calculation model could be used to estimate the erosion depth of compressor impeller material FV520B.

Spatial Patterns of Methane Oxidation and Methanotrophic Diversity in Landfill Cover Soils of Southern China

( Zi Fang Chi ),( Wen Jing Lu ),( Hong Tao Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.4

Aerobic CH4 oxidation is an important CH4 sink in landfills. To investigate the distribution and community diversity of methanotrophs and link with soil characteristics and operational parameters (e.g., concentrations of O2, CH4), cover soil samples were collected at different locations and depths from the Mengzi semi-aerobic landfill (SAL) in Yunnan Province of southern China. Specific PCR followed by denaturing gradient gel electrophoresis and realtime PCR were used to examine methanotrophs in the landfill cover soils. The results showed that different locations did harbor distinct methanotroph communities. Methanotrophs were more abundant in areas near the venting pipes because of the higher O2 concentrations. The depth of 20-25 cm, where the ratio of the CH4 to O2 was within the range from 1.3 to 8.6, was more conducive to the growth of CH4-oxidizing bacteria. Type II methanotrophs dominated in all samples compared with Type I methanotrophs, as evidenced by the high ratio of Type II to Type I methanotrophic copy numbers (from 1.76 to 11.60). The total copy numbers of methanotrophs detected were similar to other ecosystems, although the CH4 concentration was much higher in SAL cover soil. Methylobacter and Methylocystis were the most abundant Type I and Type II methanotrophs genera, respectively, in the Mengzi SAL. The results suggested that SALs could provide a special environment with both high concentrations of CH4 and O2 for methanotrophs, especially around the vertical venting pipes.

MiR-374b Promotes Proliferation and Inhibits Apoptosis of Human GIST Cells by Inhibiting PTEN through Activation of the PI3K/Akt Pathway

Zi-Wen Long,Jiang-Hong Wu,Cai-Hong,Ya-Nong Wang,Ye Zhou 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.6

Gastrointestinal stromal tumours (GIST) are the most common mesenchymal tumors of the gastrointestinal (GI) tract. In order to investigate a new treatment fot GIST, we hypothesized the effect of miR-374b targeting PTEN gene-mediated PI3K/Akt signal transduction pathway on proliferation and apoptosis of human gastrointestinal stromal tumor (GIST) cells. We obtained GIST tissues and adjacent normal tissues from 143 patients with GIST to measure the levels of miR-374b, PTEN, PI3K, Akt, caspase9, Bax, MMP2, MMP9, ki67, PCNA, P53 and cyclinD1. Finally, cell viability, cell cycle and apoptosis were detected. According to the KFGG analysis of DEGs, PTEN was involved in a variety of signaling pathways and miRs were associated with cancer development. The results showed that MiR-374b was highly expressed, while PTEN was downregulated in the GIST tissues. The levels of miR-374b, PI3K, AKT and PTEN were related to tumor diameter and pathological stage. Additionally, miR-374b increased the mRNA and protein levels of PI3K, Akt, MMP2, MMP9, P53 and cyclinD1, suggesting that miR-374b activates PI3K/Akt signaling pathway in GIST-T1 cells. Moreover, MiR-374b promoted cell viability, migration, invasion, and cell cycle entry, and inhibited apoptosis in GIST cells. Taken together, the results indicated that miR-374b pro-motes viability and inhibits apoptosis of human GIST cells by targeting PTEN gene through the PI3K/Akt signaling pathway. Thus, this study provides a new potential target for GIST treatment.

Analysis of differential proteins in two different wing‐types of female Nilaparvata lugens

An‐Wen Liang,Ting-Ting LIU,Zi-Qiang Liang,Fang-Hai Wang 한국곤충학회 2018 Entomological Research Vol.48 No.3

Nilaparvata lugens (stal) is a rice pest and contains long‐winged and short‐winged varieties, called the wing differentiation. This study compared the protein profiles of the two wing‐types in females and two wing‐disc types 5th‐instar females by two‐dimensional electrophoresis analysis. We detected 172 and 174 protein spots in adults and 5th‐instar nymphs, respectively. The number of proteins with higher content in the long‐winged (disc) individuals is much more than that in the short‐winged (disc) individuals. A total of 32 differential protein spots were found, of which 20 were successfully identified. Their main function is about catabolic process, fiber and nucleoside binding, and they constitute 52 protein–protein interactions, which is around the glycolysis as the core. These results enrich the research on the protein Level in wing development, and provide more references for future studies.

MiR-374b Promotes Proliferation and Inhibits Apoptosis of Human GIST Cells by Inhibiting PTEN through Activation of the PI3K/Akt Pathway

Long, Zi-Wen,Wu, Jiang-Hong,Hong, Cai,Wang, Ya-Nong,Zhou, Ye Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6

Gastrointestinal stromal tumours (GIST) are the most common mesenchymal tumors of the gastrointestinal (GI) tract. In order to investigate a new treatment fot GIST, we hypothesized the effect of miR-374b targeting PTEN gene-mediated PI3K/Akt signal transduction pathway on proliferation and apoptosis of human gastrointestinal stromal tumor (GIST) cells. We obtained GIST tissues and adjacent normal tissues from 143 patients with GIST to measure the levels of miR-374b, PTEN, PI3K, Akt, caspase9, Bax, MMP2, MMP9, ki67, PCNA, P53 and cyclinD1. Finally, cell viability, cell cycle and apoptosis were detected. According to the KFGG analysis of DEGs, PTEN was involved in a variety of signaling pathways and miRs were associated with cancer development. The results showed that MiR-374b was highly expressed, while PTEN was downregulated in the GIST tissues. The levels of miR-374b, PI3K, AKT and PTEN were related to tumor diameter and pathological stage. Additionally, miR-374b increased the mRNA and protein levels of PI3K, Akt, MMP2, MMP9, P53 and cyclinD1, suggesting that miR-374b activates PI3K/Akt signaling pathway in GIST-T1 cells. Moreover, MiR374b promoted cell viability, migration, invasion, and cell cycle entry, and inhibited apoptosis in GIST cells. Taken together, the results indicated that miR-374b promotes viability and inhibits apoptosis of human GIST cells by targeting PTEN gene through the PI3K/Akt signaling pathway. Thus, this study provides a new potential target for GIST treatment.

Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

Zhu Wen,Liu Tianwei,Deng Jian,Wei Cong Cong,Zhang Zi Jun,Wang Di Ming,Chen Xing Yong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet. Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats.Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome.Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments.Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

Machining characteristics of USV-MF complex assisted WEDM-LS based on multi-physical coupling

Yan Wang,Shun-wen Yao,Zi-jun Ding,Chen-zhen Wu,Wei Xiong 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.2

Ultrasonic vibration (USV) and magnetic field (MF) complex assisted techniques are combined with low speed wire electrical discharge machining (USV-MF complex assisted WEDM-LS) to improve the machining performance of conventional WEDM-LS. Firstly, the simulation results of multi-physical coupling simulation model show that under the influence of ultrasonic vibration, the working fluid fluidity is greatly enhanced and its temperature is significantly decreased. Then, a three-phase flow model of bubble, working fluid and debris in the machining area is established to investigate the effects of ultrasonic vibration on bubble and debris, and the process of bubble rupture is simulated. The simulation results show that with the increase of ultrasonic frequency, the movement of bubble and debris becomes fiercer and fiercer, when ultrasonic frequency is 30 kHz and ultrasonic amplitude is 10 μm, the bubble is collapsed. Finally, comparison of the experimental results of conventional WEDM-LS, USV assisted WEDM-LS, MF assisted WEDM-LS and USV-MF complex assisted WEDM-LS in machining TiNi-01 shape memory alloy reveals that the ratio of normal discharge and the material removal rate are both increased, the workpiece surface roughness value is decreased and the workpiece surface quality is improved in the process of USV-MF complex assisted WEDM-LS.