Participation of CCL1 in Snail-Positive Fibroblasts in Colorectal Cancer Contribute to 5-Fluorouracil/Paclitaxel Chemoresistance

Ziqian Li,Kaying Chan,Yifei Qi,Linlin Lu,Fen Ning,Mengling Wu,Haifang Wang,Yuan Wang,Shaohui Cai,Jun Du 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Cancer-associated fibroblasts (CAFs) activated by cancer cells has a central role in development and malignant biological behavior in colorectal cancer (CRC). Adult fibroblasts do not express Snail, but Snail-positive fibroblasts are discovered in the stroma of malignant CRC and reported to be the key role to chemoresistance. However, the reciprocal effect of CAFs expressed Snail to chemoresistance on CRC cells and the underlying molecular mechanisms are not fully characterized. Materials and Methods Snail-overexpressed 3T3 stable cell lines were generated by lipidosome and CT26 mixed with 3T3-Snail subcutaneous transplanted CRC models were established by subcutaneous injection. Cell Counting Kit-8, flow cytometry and western blotting assays were performed, and immunohistochemistry staining was studied. The cytokines participated in chemoresistance was validated with reverse transcriptase-polymerase chain reaction and heatmap. Results Snail-expression fibroblasts are discovered in human and mouse spontaneous CRCs. Overexpression of Snail induces 3T3 fibroblasts transdifferentiation to CAFs. CT26 co-cultured with 3T3-Snail resisted the impairment from 5-fluorouracil and paclitaxel in vitro. The subcutaneous transplanted tumor models included 3T3-Snail cells develop without restrictions even after treating with 5-fluorouracil or paclitaxel. Moreover, these chemoresistant processes may be mediated by CCL1 secreted by Snail-expression fibroblasts via transforming growth factor /nuclear factor-B signaling pathways. Conclusion Taken together, Snail-expressing 3T3 fibroblasts display CAFs properties that support 5-fluorouracil and paclitaxel chemoresistance in CRC via participation of CCL1 and suggest that inhibition of the Snail-expression fibroblasts in tumor may be a useful strategy to limit chemoresistance.

간호사의 하부요로증상 유병률: 체계적 문헌고찰

( Ziqian Wang ) 이화간호과학연구소 2022 Health & Nursing Vol.34 No.2

Purpose: The purpose of the study was to conduct a systematic literature review to understand the prevalence of lower urinary tract symptoms among nurses. Methods: The systematic literature review was conducted as described in the systematic literature review manual on prevalence and incidence from the Joanna Briggs Institute. In order to evaluate the methodological quality of the literature, the Critical Appraisal Checklist for Studies Reporting Prevalence Data tool was used. Results: A total of 14 lower urinary tract symptoms were identified: daytime frequency, urgency, nocturia, incontinence, stress incontinence, urge incontinence, complex urinary incontinence, overactive bladder syndrome, urinary hesitation, abdominal pressure voiding, hepatic colic, weak urination, urgency, and diurnal points. The prevalence varied from 1.5% to 64.2%. Conclusion: The prevalence of lower urinary tract symptoms among nurses was found to be high overall, confirming the severity of the prevalence of lower urinary tract symptoms among nurses.

Blood-Brain Barrier Disruption and Perivascular Beta-Amyloid Accumulation in the Brain of Aged Rats with Spontaneous Hypertension: Evaluation with Dynamic Contrast-Enhanced Magnetic Resonance Imaging

Yu Wang,Ruzhi Zhang,Chuanyuan Tao,Ziqian Xu,Wei Chen,Chunhua Wang,Li Song,Jie Zheng,Fabao Gao 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.3

Objective: Whether blood-brain barrier (BBB) disruption induced by chronic spontaneous hypertension is associated with beta-amyloid (Aβ) accumulation in the brain remains poorly understood. The purpose of this study was to investigate the relationship between BBB disruption and Aβ influx and accumulation in the brain of aged rats with chronic spontaneous hypertension. Materials and Methods: Five aged spontaneously hypertensive rats (SHRs) and five age-matched normotensive Wistar-Kyoto (WKY) rats were studied. The volume transfer constant (Ktrans) obtained from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) was used to evaluate BBB permeability in the hippocampus and cortex in vivo. The BBB tight junctions, immunoglobulin G (IgG), Aβ, and amyloid precursor protein (APP) in the hippocampus and cortex were examined with immunohistochemistry. Results: As compared with WKY rats, the Ktrans values in the hippocampus and cortex of the SHRs increased remarkably (0.316 ± 0.027 min-1 vs. 0.084 ± 0.017 min-1, p < 0.001 for hippocampus; 0.302 ± 0.072 min-1 vs. 0.052 ± 0.047 min-1, p < 0.001 for cortex). Dramatic occludin and zonula occludens-1 losses were detected in the hippocampus and cortex of SHRs, and obvious IgG exudation was found there. Dramatic Aβ accumulation was found and limited to the area surrounding the BBB, without extension to other parenchyma regions in the hippocampus and cortex of aged SHRs. Alternatively, differences in APP expression in the hippocampus and cortex were not significant. Conclusion: Blood-brain barrier disruption is associated with Aβ influx and accumulation in the brain of aged rats with chronic spontaneous hypertension. DCE-MRI can be used as an effective method to investigated BBB damage.

Self-Gated Late Gadolinium Enhancement at 7T to Image Rats with Reperfused Acute Myocardial Infarction

Lei Wang,Yushu Chen,Bing Zhang,Wei Chen,Chunhua Wang,Li Song,Ziqian Xu,Jie Zheng,Fabao Gao 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.2

Objective: A failed electrocardiography (ECG)-trigger often leads to a long acquisition time (TA) and deterioration in image quality. The purpose of this study was to evaluate and optimize the technique of self-gated (SG) cardiovascular magnetic resonance (CMR) for cardiac late gadolinium enhancement (LGE) imaging of rats with myocardial infarction/reperfusion. Materials and Methods: Cardiovascular magnetic resonance images of 10 rats were obtained using SG-LGE or ECG with respiration double-gating (ECG-RESP-gating) method at 7T to compare differences in image interference and TA between the two methods. A variety of flip angles (FA: 10°−80°) and the number of repetitions (NR: 40, 80, 150, and 300) were investigated to determine optimal scan parameters of SG-LGE technique based on image quality score and contrast-to-noise ratio (CNR). Results: Self-gated late gadolinium enhancement allowed successful scan in 10 (100%) rats. However, only 4 (40%) rats were successfully scanned with the ECG-RESP-gating method. TAs with SG-LGE varied depending on NR used (TA: 41, 82, 154, and 307 seconds, corresponding to NR of 40, 80, 150, and 300, respectively). For the ECG-RESP-gating method, the average TA was 220 seconds. For SG-LGE images, CNR (42.5 ± 5.5, 43.5 ± 7.5, 54 ± 9, 59.5 ± 8.5, 56 ± 13, 54 ± 8, and 41 ± 9) and image quality score (1.85 ± 0.75, 2.20 ± 0.83, 2.85 ± 0.37, 3.85 ± 0.52, 2.8 ± 0.51, 2.45 ± 0.76, and 1.95 ± 0.60) were achieved with different FAs (10°, 15°, 20°, 25°, 30°, 35°, and 40°, respectively). Optimal FAs of 20°−30° and NR of 80 were recommended. Conclusion: Self-gated technique can improve image quality of LGE without irregular ECG or respiration gating. Therefore, SG-LGE can be used an alternative method of ECG-RESP-gating.

Human Recombinant Apyrase Therapy Protects Against Myocardial Ischemia/Reperfusion Injury and Preserves Left Ventricular Systolic Function in Rats, as Evaluated by 7T Cardiovascular Magnetic Resonance Imaging

Xu Ziqian,Chen Wei,Zhang Ruzhi,Wang Lei,Chen Ridong,Zheng Jie,Gao Fabao 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.6

Objective: The occurrence of intramyocardial hemorrhage (IMH) and microvascular obstruction (MVO) in myocardial infarction (MI), known as severe ischemia/reperfusion injury (IRI), has been associated with adverse remodeling. APT102, a soluble human recombinant ecto-nucleoside triphosphate diphosphohydrolase-1, can hydrolyze extracellular nucleotides to attenuate their prothrombotic and proinflammatory effects. The purpose of this study was to temporally evaluate the therapeutic effect of APT102 on IRI in rats and to elucidate the evolution of IRI in the acute stage using cardiovascular magnetic resonance imaging (CMRI). Materials and Methods: Fifty-four rats with MI, induced by ligation of the origin of the left anterior descending coronary artery for 60 minutes, were randomly divided into the APT102 (n = 27) or control (n = 27) group. Intravenous infusion of APT102 (0.3 mg/kg) or placebo was administered 15 minutes before reperfusion, and then 24 hours, 48 hours, 72 hours, and on day 4 after reperfusion. CMRI was performed at 24 hours, 48 hours, 72 hours, and on day 5 post-reperfusion using a 7T system and the hearts were collected for histopathological examination. Cardiac function was quantified using cine imaging and IMH/edema using T2 mapping, and infarct/MVO using late gadolinium enhancement. Results: The extent of infarction (p < 0.001), edema (p < 0.001), IMH (p = 0.013), and MVO (p = 0.049) was less severe in the APT102 group than in the control group. IMH size at 48 hours was significantly greater than that at 24 hours, 72 hours, and 5 days after reperfusion (all p < 0.001). The left ventricular ejection fraction (LVEF) was significantly greater in the APT102 group than in the control group (p = 0.006). There was a negative correlation between LVEF and IMH (r = -0.294, p = 0.010) and a positive correlation between IMH and MVO (r = 0.392, p < 0.001). Conclusion: APT102 can significantly alleviate damage to the ischemic myocardium and microvasculature. IMH size peaked at 48 hours post reperfusion and IMH is a downstream consequence of MVO. IMH may be a potential therapeutic target to prevent adverse remodeling in MI.

Extremely Strong Western Pacific Subtropical High in May 2021 Following a La Niña Event: Role of the Persistent Convective Forcing over the Indian Ocean

Minling Ke,Ziqian Wang,Weijuan Pan,Haolin Luo,Song Yang,Ruyue Guo 한국기상학회 2023 Asia-Pacific Journal of Atmospheric Sciences Vol.59 No.1

The variation of the western Pacific subtropical high (WPSH) significantly influences the weather and climate in East Asia. El Niño-Southern Oscillation (ENSO) is considered as one of the most important factors for the abnormal activity of the WPSH. An El Niño event tends to result in an anticyclonic anomaly over the western Pacific in the following spring and summer, leading to a westward-shifted and stronger WPSH. Opposite features can be observed for a La Niña event. Following the typical La Niña event in the winter of 2020/2021, an abnormal cyclonic circulation routinely appeared over the western Pacific in the beginning of 2021, but it was suddenly replaced by an obviously abnormal anticyclone in May. This unanticipated change induced an extremely strong WPSH and posed a challenge for the regional climate prediction. A careful examination of the tropical Indian Ocean revealed a significant abnormal warming process from April to May in 2021, with a peak of positive sea surface temperature anomaly (SSTA) in early May. Consequently, persistent atmospheric convective activity was stimulated by the positive SSTA, accompanied by remarkable and eastward-moving diabatic heating in the tropical Indian Ocean. The convective heating aroused significant easterly anomalies in the form of a Kelvin wave response of the Gill-type mode over the equatorial region from the western Pacific to the eastern Indian Ocean, which induced an abnormal anticyclone through a further positive circulation–convection feedback over the western Pacific. Additional experiments with the LBM model further verify that the persistent convective forcing over the tropical Indian Ocean is responsible for the extremely strong WPSH in May 2021, although during an antecedent La Niña event.

Sensitivity of Extreme Temperature Events to Urbanization in the Pearl River Delta Region

Zhixiang Xiao,Ziqian Wang,Weijuan Pan,Yongli Wang,Song Yang 한국기상학회 2019 Asia-Pacific Journal of Atmospheric Sciences Vol.55 No.3

The urbanization effect on extreme temperature related to heat wave events in the Pearl River Delta (PRD) of China is investigated using a regional model. Several experiments with different urbanization levels are carried out. Results indicate that the air temperature of heat wave events is sensitive to urban land cover and the urbanized warming intensifies along with urban expansion. The regional model performs better on the simulations of maximum and minimum 2-m air temperature when urban land cover is close to the observed value. Because of the urbanization effect, the surface skin temperature shows a maximum warming (~2.5 °C) at daytime, while the maximum warming of 2-m air temperature (~1.5 °C) occurs at nighttime. Consequently, an increased (a decreased) diurnal range in surface skin temperature (2-m air temperature) is induced. According to the diagnostic equations, the 2-m air temperature is not only related to surface skin temperature, but also regulated by surface sensible heat flux. At daytime, although the increased solar absorption and the decreased upward latent heat flux (due to the deficit of surface evaporation) sharply enhance the surface skin temperature in the higher urbanization experiment, the simultaneously increased sensible heat flux suppresses the 2-m air temperature. At nighttime, however, the difference in surface sensible heat flux is relatively small, thus 2-m air temperature largely depends on surface skin temperature influenced by ground heat release. This study emphasizes the different variations and physical mechanisms of surface skin temperature and 2-m air temperature under the urbanization in the PRD region.

A Study on the Development of Contemporary Chinese Animations Based on the Analysis of Three New Animation Films

Yin Jun,Wang Ziqian 한중경제문화학회 2019 한중경제문화연구 Vol.12 No.-

Computational design towards energy efficient optimization in overconstrained robotic limbs

Gu Yuping,Wang Ziqian,Feng Shihao,Sun Haoran,Lu Haibo,Pan Jia,Wan Fang,Song Chaoyang 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.5

Legged robots are constantly evolving, and energy efficiency is a major driving factor in their design. However, combining mechanism efficiency and trajectory planning can be challenging. This work proposes a computational optimization framework for optimizing leg design during basic walking while maximizing energy efficiency. We generalize the robotic limb design as a four-bar linkage-based design pool and optimize the leg using an evolutionary algorithm. The leg configuration and design parameters are optimized based on user-defined objective functions. Our framework was validated by comparing it to measured data on our prototype quadruped robot for forward trotting. The Bennett robotic leg was advantageous for omni-directional locomotion with enhanced energy efficiency.