Fuzzy Partition based Similarity Measure for Spectral Clustering

Yifang Yang,Yuping Wang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

The efficiency of spectral clustering depends heavily on the similarity measure adopted. A widely used similarity measure is the Gaussian kernel function where Euclidean distance is used. Unfortunately, the result of spectral clustering is very sensitive to the scaling parameter and the Euclidean distance is usually not suitable to the complex distribution data. In this paper, a spectral clustering algorithm based on fuzzy partition similarity measure ( FPSC) is presented to solve the problem that result of spectral clustering is very sensitive to scaling parameter. The proposed algorithm is steady extremely and hardly affected by the scaling parameter. Experiments on three benchmark datasets, two synthetic texture images are made, and the results demonstrate the effectiveness of the proposed algorithm.

Static behavior of steel tubular structures considering local joint flexibility

Yamin Wang,Yong-Bo Shao,Yifang Cao 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.24 No.4

As a thin-walled structure, local joint flexibility (LJF) in a tubular structure is prominent, and it may produce significant effect on the static performance for the overall structure. This study presents a simplified analytical model to analyze the static behavior for a steel tubular structure with LJF. The presented model simplifies a tubular structure into a frame model consisted of beam elements with considering the LJFs at the connections between any two elements. Theoretical equations of the simplified analytical model are deduced. Through comparison with 3-D finite element results of two typical planar tubular structures consisted of T- and Y-joints respectively, the presented method is proved to be accurate. Furthermore, the effect of LJF on the overall performance of the two tubular structures (including the deflection and the internal forces) is also investigated, and it is found from analyses of internal forces and deformation that a rigid connection assumption in a frame model by using beam elements in finite element analysis can provide unsafe and inaccurate estimation.

Inactivation of epithelial sodium ion channel molecules serves as effective diagnostic biomarkers in clear cell renal cell carcinoma

Zheng Qian,Wang Yifang,Zhao Ran,Han Peipei,Zhao Jun,Li Limei,Zhou Xiaohui,Li Ping,Mo Yingxi,Pan Xinli,Luo Wenqi,Zhou Xiaoying 한국유전학회 2023 Genes & Genomics Vol.45 No.7

Background Non-voltage-gated sodium channel, also known as the epithelial sodium channel (ENaC), formed by heteromeric complexes consisting of SCNN1A, SCNN1B, and SCNN1G, is responsible for maintaining sodium ion and body fluid homeostasis in epithelial cells. However, no systematic study of SCNN1 family members has been conducted in renal clear cell carcinoma (ccRCC) to date. Objective To investigate the abnormal expression of SCNN1 family in ccRCC and its potential correlation with clinical parameters. Methods The transcription and protein expression levels of SCNN1 family members in ccRCC were analyzed based on the TCGA database, and were confirmed by quantitative RT-PCR and immunohistochemical staining assays, respectively. The area under curve (AUC) was used to evaluate the diagnostic value of SCNN1 family members for ccRCC patients. Results The mRNA and protein expression of SCNN1 family members was significantly downregulated in ccRCC compared with normal kidney tissues, which might be due to DNA hypermethylation in the promoter region. It is worth noting that the AUC of SCNN1A, SCNN1B, and SCNN1G were 0.965, 0.979, and 0.988 based on the TCGA database (p < 0.0001), respectively. The diagnostic value was even higher when combing these three members together (AUC = 0.997, p < 0.0001). Intriguingly, the mRNA level of SCNN1A was significantly lower in females compared with males, while SCNN1B and SCNN1G were increased with the progression of ccRCC and remarkably associated with a worse outcome for patients. Conclusion The aberrantly decrease of SCNN1 family members might serve as valuable biomarkers for the diagnosis of ccRCC.

A Comparison of Eu-Doped -Fe2O3 Nanotubes and Nanowires for Acetone Sensing

Yali Cheng,Yifang Wang,Jinbao Zhang,Haiying Li,Li Liu,Yu Lina,Liting Du,Haojie Duan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.11

Pure and Eu-doped (1.0, 3.0, 5.0 wt.%) α-Fe2O3 (PFO and EFO) nanotubes and nanowires have been successfully synthesized through the combination of electrospinning and calcination techniques. The structures, morphologies and chemical compositions of the as-obtained products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric and differential scanning calorimetry (TG-DSC) and energy dispersive spectrum (EDS), respectively. To demonstrate the superior gas sensing performance of the doped nanotubes, a contrastive gas sensing study between PFO (EFO) nanotubes and nanowires was performed. It turned out that Eu doping could magnify the impact of morphology on gas sensitivity. Specifically, at the optimum operating temperature of 240 ℃, the response value of PFO nanotubes to 100 ppm acetone is slightly higher than that of nanowires (3.59/2.20). EFO (3.0 wt.%) nanotubes have a response of 84.05, which is almost 2.7 times as high as that of nanowires (31.54). Moreover, they possess more rapid response/recovery time (11 s and 36 s, respectively) than nanowires (17 s and 40 s, respectively). The lowest detection limit for acetone is 0.1 ppm and its response is 2.15. In addition, both of EFO nanotubes and nanowires sensors have a good linearity (0.1–500 ppm) and favorable selectivity in acetone detection.

중국 소수민족 좡족(壯族) 복식의 특성을 활용한 패션디자인

왕이팡,이진경,이연희,Wang, Yifang,Lee, Jinkyung,Lee, Younhee 복식문화학회 2017 服飾文化硏究 Vol.25 No.5

This study analyzes the characteristics of clothes worn by the Zhuang in order to produce new fashion designs, and to propose diverse new directions in fashion design. Research was conducted using a bibliographic survey on the cultural background, characteristics, and relevant techniques of the Zhuang costume and that of minority races in China. This study deploys four styles of design for women's wear. With the inspiration of the traditional Zhuang costume, black and blue were the colors mainly used for the Zhuang people and the material was mostly denim. Denim blends in well for the contemporary facilitation of the Zhuang costume, which is known for knitting technique, fur and hemp fabric as patchwork, and embroidery works. It is appropriate to express the joyful and happy mind of Zhuang people with extraordinary colors, exaggerated silhouettes, and various decorations. Images of nature, such as the sun, mountains, rivers, water, fish etc., expressed the nature worship of the Zhuang in contemporary design, representing the simple life and peaceful mind. This research develops a new fashion design and displays the possibility for diverse design development through new insight in contemporary fashion design.

Reactor Neutrinos

Kim, Soo-Bong,Lasserre, Thierry,Wang, Yifang Hindawi Limited 2013 Advances in high energy physics Vol.2013 No.-

<P>We review the status and the results of reactor neutrino experiments. Short-baseline experiments have provided the measurement of the reactor neutrino spectrum, and their interest has been recently revived by the discovery of the reactor antineutrino anomaly, a discrepancy between the reactor neutrino flux state of the art prediction and the measurements at baselines shorter than one kilometer. Middle and long-baseline oscillation experiments at Daya Bay, Double Chooz, and RENO provided very recently the most precise determination of the neutrino mixing angle<SUB>θ13</SUB>. This paper provides an overview of the upcoming experiments and of the projects under development, including the determination of the neutrino mass hierarchy and the possible use of neutrinos for society, for nonproliferation of nuclear materials, and geophysics.</P>

Epidemiological investigation and phylogenetic analysis of Classical Swine Fever virus in Yunnan province from 2015 to 2021

Jun Yao,Linlin Su,Qiaoping Wang,Lin Gao,Jiarui Xie,Yuwen He,Xianghua Shu,Chunlian Song,Jun Chai,Yifang Zhang,Shibiao Yang 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.4

Background: Classical swine fever virus (CSFV), the causative agent of classical swine fever (CFS), is a highly contagious disease that poses a serious threat to Chinese pig populations. Objectives: Many provinces of China, such as Shandong, Henan, Hebei, Heilongjiang, and Liaoning provinces, have reported epidemics of CSFV, while the references to the epidemic of CSFV in Yunnan province are rare. This study examined the epidemic characteristics of the CSFV in Yunnan province. Methods: In this study, 326 tissue samples were collected from different regions in Yunnan province from 2015 to 2021. A reverse transcription-polymerase chain reaction (RT-PCR), sequences analysis, and phylogenetic analysis were performed for the pathogenic detection and analysis of these 326 clinical specimens. Results: Approximately 3.37% (11/326) of specimens tested positive for the CSFV by RT-PCR, which is lower than that of other regions of China. Sequence analysis of the partial E2 sequences of eleven CSFV strains showed that they shared 89.0–100.0% nucleotide (nt) and 95.0–100.0% amino acid (aa) homology, respectively. Phylogenetic analysis showed that these novel isolates belonged to the subgenotypes 2.1c and 2.1d, with subgenotype 2.1c being predominant. Conclusions: The CSFV was sporadic in China’s Yunnan province from 2015 to 2021. Both 2.1c and 2.1d subgenotypes were found in this region, but 2.1c was dominant.

Epigenetic inactivation of ACAT1 promotes epithelial-mesenchymal transition of clear cell renal cell carcinoma

Han Peipei,Wu Shu,Li Limei,Li Danping,Zhao Jun,Zhang Haishan,Wang Yifang,Zhong Xuemin,Zhang Zhe,Li Ping,Matskova Liudmila,Zhou Xiaoying 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Background: Acetyl-CoA acyltransferase 1 (ACAT1) is a key enzyme catalyzing the production of mitochondrial ketone bodies. We have shown that ACAT1 is down-regulated in kidney renal clear cell carcinoma (KIRC) previously. Objective: To investigate the reasons for downregulation of ACAT1 in KIRC and explore the underlying mechanisms involved in metastatic inhibition regulated by ACAT1. Methods: The Gene Expression Omnibus (GEO) database was queried for meta-analysis of ACAT1 mRNA expression in KIRC. The UALCAN website was used to compare the methylation levels of the ACAT1 promoter region in KIRC and normal tissues. RT-qPCR was used to quantitate ACAT1 transcription levels. The GCBI and Tarbase V.8 databases were used to predict miRNAs that may target the mRNA of ACAT1. The correlation between mRNA expression of ACAT1, MMP7 (matrix metallopeptidase 7), CDH1 (E-cadherin), EpCAM (epithelial cell adhesion molecule), and VIM (vimentin) was analyzed. Extracellular MMP7 protein was quantitated using an ELISA assay. Results: The methylation level of the ACAT1 promoter region in KIRC was significantly higher than that in the normal kidney tissues. The ACAT1 mRNA expression in the KIRC cell lines was restored after treatment with 5-aza-dC (p < 0.05). MiR-21-5p is a conserved microRNA targeting ACAT1. It is expressed at a significantly higher level in KIRC than in normal tissues (p < 0.001). MiR-21-5p miRNA expression negatively correlates with ACAT1 mRNA expression. The expression of miR-21-5p is higher at the T3-T4 stages and in the histologic grades G3-G4. Patients with high miR-21-5p expression tended to have lower overall survival, suggesting that miR-21-5p could serve as a potentially valuable diagnostic biomarker for KIRC (AUC = 0.957; p < 0.001). A mimetic of miR-21-5p inhibited the expression of ACAT1 mRNA and protein. In addition, ACAT1 mRNA expression positively correlates with CDH1 and EpCAM but is negatively correlated with VIM. Overexpression of ACAT1 suppresses the secretion of MMP7 in KIRC cells. Conclusion: Expression of ACAT1 in KIRC is controlled at two levels, firstly by the hypermethylation of the ACAT1 promoter region and secondly by overexpression of miR-21-5p. Downregulation of ACAT1 expression correlates with epithelial-mesenchymal transition (EMT).

RhGLP-1 (7-36) protects diabetic rats against cerebral ischemia-reperfusion injury via up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD

Yi Fang,Xiaofang Liu,Libo Zhao,Zhongna Wei,Daoli Jiang,Hua Shao,Yannan Zang,Jia Xu,Qian Wang,Yang Liu,Ye Peng,Xiaoxing Yin 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7-36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7-36) (10, 20, 40 μg/kg i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7- 36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7-36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7-36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.