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- 저자 : Wang Xiaoge (검색결과 5 건)
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Cloning and expression of a Chk1 gene in Daphnia pulex during different modes of reproduction
Xiaoge Guo,Shanliang Xu,Xuemei Yan,Wei Zhou,Xinyu Dai,Xiu Zou,Chunlin Wang,Danli Wang,Yunlong Zhao 한국유전학회 2015 Genes & Genomics Vol.37 No.9
The full-length cDNA of a Chk1 gene (DpChk1) was cloned from Daphnia pulex using RACE method. This cDNA is 1767 bp in length, consisting of a 1497 bp open reading frame encoding 498 amino acid protein. The deduced DpChk1 protein sequence contains phosphorylation sites, three conserved Ser-Gln and Thr- Gln motifs and shares 51–55 % similarity with A. pisum, M. rotundata, D. melanogaster, A. echinatior, B. terrestris, R. pulchellus and M. occidentalis, respectively. Gene expression analysis showed that Chk1 is differentially expressed in D. pulex during different reproductive modes. DpChk1 expression in sexually-reproducing females and males is significantly higher than in parthenogenetic Daphnia. Whole-mount in situ hybridization revealed that DpChk1 was expressed during two kinds of reproductive modes. In parthenogenetic females, hybridization signals were found in the antennae and thoracic limb, whereas expression levels in the corresponding sites of sexuallyreproducing females were relatively strong, DpChk1 could not be detected in the gonads of ephippial and parthenogenetic females. Taken together, these different reproductive stages and sex specific expression patterns are regulated temporally and spatially. We speculate that DpChk1 may be involved in conversion between different stages of reproduction and in sexual differentiation in D. pulex.
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Wang Xulei,Li Yikang,Huang Yabo,Zhang Yalong,Wang Pei,Guan Li,He Xinbo,Liu Rongjun,Qu Xuanhui,Wu Xiaoge 한국탄소학회 2024 Carbon Letters Vol.34 No.6
Diamond/SiC composites were prepared by vacuum silica vapor-phase infiltration of in situ silicon–carbon reaction, and the thermophysical properties of the composites were modulated by controlling diamond graphitizing. The effects of diamond surface state and vacuum silicon infiltration temperature on diamond graphitization were investigated, and the micro-morphology, phase composition, and properties of the composites were observed and characterized. The results show that diamond pretreatment can reduce the probability of graphitizing; when the penetration temperature is greater than 1600 °C, the diamond undergoes a graphitizing phase transition and the micro-morphology presents a lamellar shape. The thermal conductivity, density, and flexural strength of the composites increased and then decreased with the increase of penetration temperature in the experimentally designed range of penetration temperature. The variation of thermal expansion coefficients of composites prepared with different penetration temperatures ranged from 0.8 to 3.0 ppm/K when the temperature was between 50 and 400 °C.
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Fast fluorescent blood sugar sensing using phenylboronic acid functionalized N, S-doped carbon dots
Wang Xiaoge,Fan Xiangze,Zhang Bowen,Zhan Mengke,Zhu Jinhua,Hu Weiping,Liu Xiuhua 한국탄소학회 2024 Carbon Letters Vol.34 No.5
A phenylboric acid functionalized carbon dot (2-FPBA-CD) for rapid fluorescent sensing of glucose in blood was synthesized by simply mixing N, S-doped carbon dots (CDs) with phenylboric acid at room temperature. At pH 7.4, the response of 2-FPBA-CD to glucose could reach equilibrium in a very short time (10 min), with a wide responsive linear range of 19.70 µM to 2.54 mM, which can be applied to the detection of glucose in serum. The mechanism studies showed that the layered carbon film of 2-FPBA-CD aggregated after adding glucose, thereby leading to the fluorescence quenching of 2-FPBA-CD.
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Spatiotemporal attention aided graph convolution networks for dynamic spectrum prediction
Li Yue,Shen Bin,Wang Xin,Huang Xiaoge 한국통신학회 2024 ICT Express Vol.10 No.4
To solve the spectrum scarcity problem, dynamic spectrum access (DSA) technology has emerged as a promising solution. Effectively implementing DSA demands accurate and efficient spectrum prediction. However, complex spatiotemporal correlation and heterogeneity in spectrum observations usually make spectral prediction arduous and even ambiguous. In this letter, we propose a spectrum prediction method based on an attention-aided graph convolutional neural network (AttGCN) to capture features in both spatial and temporal dimensions. By leveraging the attention mechanism, the AttGCN adapts its attention weights at different time steps and spatial positions, thus enabling itself to seize changes in spatiotemporal correlations dynamically. Simulation results show that the proposed spectrum prediction method performs better than baseline algorithms in long-term forecasting tasks.
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Yanke Chen,Jing Luan,Ting Jiang,Donghui Cai,Chao Sun,Xiaofei Wang,Xiaoge Zhao,Xingchun Gou 대한암학회 2021 Cancer Research and Treatment Vol.53 No.2
Purpose Bone destruction and pain caused by cancer is one of the most devastating complications of cancer patients with bone metastases, and it seriously affects the quality of patients’ life. Extracellular matrix metalloproteinase inducer (EMMPRIN) is a cell adhesion molecule with increased expression in a variety of tumors. This study focused to clarify the specific function of EMMPRIN in bone metastasis of breast cancer.Materials and Methods Adenovirus with shRNA-EMMPRIN was transfected into MRMT-1 rat breast carcinoma cells, and the MRMT-1 cells with different expression levels of EMMPRIN were implanted into the bone marrow cavity of rat tibia. Next, the effect of down-regulation of EMMPRIN was evaluated as follows: bone damage was detected by X-ray radiological and tartrate-resistant acid phosphatase staining; the tumor burden was evaluated by hematoxylin and eosin staining; the test of pain-related behaviors was assessed used the bilateral paw withdrawal mechanical threshold; and the levels of secretory factors in tumor conditioned medium were determined by using enzyme-linked immunosorbent assay.ResultsWe found that down-regulation of EMMPRIN in tumor cells can simultaneously reduce tumor burden, relieve cancer-induced bone destruction and pain. ConclusionMaterials and Methods EMMPRIN is expected to be a therapeutic target for relieving bone metastasis of breast cancer and alleviating cancerinduced bone destruction and pain. The method of targeting EMMPRIN may be a promising strategy for the treatment of cancer in the future.
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