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Wang, Xin,Wang, Chao,Sun, Yu-Ting,Sun, Chuan-Zhen,Zhang, Yue,Wang, Xiao-Hua,Zhao, Kai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1
Currently, taxol is mainly extracted from the bark of yews; however, this method can not meet its increasing demand on the market because yews grow very slowly and are a rare and endangered species belonging to first-level conservation plants. Recently, increasing efforts have been made to develop alternative means of taxol production; microbe fermentation would be a very promising method to increase the production scale of taxol. To determine the activities of the taxol extracted from endophytic fungus N. sylviforme HDFS4-26 in inhibiting the growth and causing the apoptosis of cancer cells, on comparison with the taxol extracted from the bark of yew, we used cellular morphology, cell counting kit (CCK-8) assay, staining (HO33258/PI and Giemsa), DNA agarose gel electrophoresis and flow cytometry (FCM) analyses to determine the apoptosis status of breast cancer MCF-7 cells, cervical cancer HeLa cells and ovarian cancer HO8910 cells. Our results showed that the fungal taxol inhibited the growth of MCF-7, HeLa and HO8910 cells in a dose-and time-dependent manner. IC50 values of fungal taxol for HeLa, MCF-7 and HO8910 cells were $0.1-1.0{\mu}g/ml$, $0.001-0.01{\mu}g/ml$ and $0.01-0.1{\mu}g/ml$, respectively. The fungal taxol induced these tumor cells to undergo apoptosis with typical apoptotic characteristics, including morphological changes for chromatin condensation, chromatin crescent formation, nucleus fragmentation, apoptotic body formation and G2/M cell cycle arrest. The fungal taxol at the $0.01-1.0{\mu}g/ml$ had significant effects of inducing apoptosis between 24-48 h, which was the same as that of taxol extracted from yews. This study offers important information and a new resource for the production of an important anticancer drug by endofungus fermentation.
Stable and Recyclable SERS Substrates Based on Au-Loaded PET Nanocomposite Superhydrophobic Surfaces
Hua-Xiang Chen,Yu-Ting Wang,Ting-Ting You,Jin Zhai,Peng-Gang Yin 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5
Novel surface-enhanced Raman scattering (SERS) substrates with stable and recyclable properties have been prepared by assembling gold nanoparticles-loaded PET (AuNPs/PET) nanocomposite superhydrophobic surfaces. After a physical vapor deposition process, the AuNPs/PET surfaces with vast plasmonic "hot spots" showed superhydrophobic properties, and it can hold target molecules droplets for rapid SERS detection. From blown off droplets and rinsed substrates with water after detection, we found that no probe molecules remained on the surfaces from Raman spectra. The prepared substrates were not contaminated in the detection process. Furthermore, the new SERS substrates were used for rapidly detecting droplets of crystal violet (CV) and the lowest detection concentration was about 1 x 10 -9 M. The as-prepared AuNPs/PET substrates also have good performance in terms of reproducibility and recyclability.
Ying-Hua Liang,Ping-Zhan Si,Ting-Ting Qi,Xin-You Wang,Fei-Yang Wang,Qiong Wu,Hong-Liang Ge,Jihoon Park,Chul-Jin Choi 한국자기학회 2024 Journal of Magnetics Vol.29 No.1
Both Mn₄C (=Mn₃MnC) and Mn₃GaC have been studied previously. However, the reports on Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) with intermediate compositions are very rare. In this work, the structure and magnetic properties of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C prepared by using solid state reaction were studied systematically. High purity anti-perovskitetype Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C were obtained in the composition range of 0 ≤ x ≤ 0.5, above which Mn₂₃C₆ precipitates and the fraction of Mn₂₃C₆ in the samples increases with increasing x. The structural stability, lattice parameters, and room temperature saturation magnetization of ferromagnetic Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) decreases with increasing x. The Curie temperature of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) increases with increasing x. Most Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C with varied x exhibit near-zero coercivity and zero remanent magnetization. This work indicates that the temperature coefficient of magnetization of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C may be tuned by tuning the fraction of the Ga atoms.
Experimental Study and Parameter Analysis of L-shaped Composite Column under Axial Loading
Ting Zhou,Minyang Xu,Xiaodun Wang,Zhi-Hua Chen,Ying Qin 한국강구조학회 2015 International Journal of Steel Structures Vol.15 No.4
A special-shaped column composed of concrete-filled steel tubes (SCFST column) is experimentally investigated in this paper. The SCFST column is composed of several small-sized concrete-filled square tubular columns (mono-columns) connected by connection plates. This kind of column is proposed because it can increase the usable space in residential buildings. The axial loading behavior of L-shaped SCFST columns is experimentally investigated. The failure mode, strain distribution, cooperation of mono-columns, bearing capacity, and the effect of a concrete core on column behavior were tested. As concluded, the SCFST column can be regarded as a useful kind of column. A finite element analysis model was proposed according to the experimental results, and the influence of connection plate size on bearing capacity was studied using the finite element model. Finally, proposed were the optimal width and thickness of connection plates for improving the design of the SCFST column.
Ting Deng,Kaijiang Liu,Liang Chen,Xiao-jun Chen,Hua Wen Li,Hongyan Guo,Huijiao Zhang,Libing Xiang,Xin Feng,Xiaoyu Wang,Hextan Y. S. Ngan,Jianguo Zhao,Dongling Zou,Qing Liu,Jihong Liu 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.3
Background: The Lymphadenectomy in Ovarian Neoplasms (LION) study revealed that systemic lymphadenectomy did not bring survival benefit for advanced ovarian cancer patients with clinically normal lymph nodes and was associated with a higher incidence of operative complications. However, there is no consensus on whether lymphadenectomy has survival benefit or not in early epithelial ovarian cancer (EOC). Methods: We designed the LOVE study, a multicenter, randomized controlled, phase III trial to compare the efficacy and safety of comprehensive staging surgery with or without lymphadenectomy in stages IA-IIB EOC and fallopian tube carcinomas (FTC). The hypothesis is that the oncological outcomes provided by comprehensive staging surgery without lymphadenectomy are non-inferior to those of conventional completion staging surgery in early-stage EOC and FTC patients who have indications for post-operative adjuvant chemotherapy. Patients assigned to experimental group will undergo comprehensive staging surgery, but lymphadenectomy. Patients assigned to comparative group will undergo completion staging surgery including systematic pelvic and para-aortic lymphadenectomy. All subjects will receive 3–6 cycles of standard adjuvant chemotherapy. Major inclusion criteria are pathologic confirmed stage IA-IIB EOC or FTC, and patients have indications for adjuvant chemotherapy either confirmed by intraoperative fast frozen section or previous pathology after an incomplete staging surgery. Major exclusion criteria are non-epithelial tumors and low-grade serous carcinoma. Patients with severe rectum involvement which lead to partial rectum resection will be excluded. The sample size is 656 subjects. Primary endpoint is disease-free survival.
Experimental seismic behaviour of L-CFST column to H-beam connections
Wang Zhang,Zhi-Hua Chen,Qingqing Xiong,Ting Zhou,Xian Rong,Yansheng Du 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.26 No.6
In this study, the seismic performance of the connections between L-shaped columns composed of concrete-filled steel tubes (L-CFST columns) and H-beams used in high-rise steel frame structures was investigated. Seven full-scale specimens were tested under quasi-static cyclic loading. The variables studied in the tests included the joint type, the axial compression ratio, the presence of concrete, the width-to-thickness ratio and the internal extension length of the side plates. The hysteretic response, strength degradation, stiffness degradation, ductility, plastic rotation capacity, energy dissipation capacity and the strain distribution were evaluated at different load cycles. The test results indicated that both the corner and exterior joint specimens failed due to local buckling and crack within the beam flange adjacent to the end of the side plates. However, the failure modes of the interior joint specimens primarily included local buckling and crack at the end plates and curved corners of the beam flange. A design method was proposed for the flexural capacity of the end plate connection in the interior joint. Good agreement was observed between the theoretical and test results of both the yield and ultimate flexural capacity of the end plate connection.
Wang Hua-Chuan,Chen Bin,Li Ting-Jing 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2
A total of five species of the genus Ectopioglossa is reviewed from China, of which two new species are described and illustrated. These two new species are Ectopioglossa trimacula sp. nov. and E. convexa sp. nov., collected from Guangxi and Yunnan, China, respectively. Among other three nomial species, E. sublaevis (Smith, 1857) is new to China. An updated key to the Oriental species is also provided.
Evaluation of porcine urine-derived cells as nuclei donor for somatic cell nuclear transfer
Yu-Ting Zhang,Wang Yao,Meng-Jia Chai,Wen-Jing Liu,Yan Liu,Zhong-Hua Liu,Xiao-Gang Weng 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.2
Background: Somatic cell nuclear transfer (SCNT) is used widely in cloning, stem cell research, and regenerative medicine. The type of donor cells is a key factor affecting the SCNT efficiency. Objectives: This study examined whether urine-derived somatic cells could be used as donors for SCNT in pigs. Methods: The viability of cells isolated from urine was assessed using trypan blue and propidium iodide staining. The H3K9me3/H3K27me3 level of the cells was analyzed by immunofluorescence. The in vitro developmental ability of SCNT embryos was evaluated by the blastocyst rate and the expression levels of the core pluripotency factor. Blastocyst cell apoptosis was examined using a terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. The in vivo developmental ability of SCNT embryos was evaluated after embryo transfer. Results: Most sow urine-derived cells were viable and could be cultured and propagated easily. On the other hand, most of the somatic cells isolated from the boar urine exhibited poor cellular activity. The in vitro development efficiency between the embryos produced by SCNT using porcine embryonic fibroblasts (PEFs) and urine-derived cells were similar. Moreover, The H3K9me3 in SCNT embryos produced from sow urine-derived cells and PEFs at the four-cell stage showed similar intensity. The levels of Oct4, Nanog, and Sox2 expression in blastocysts were similar in the two groups. Furthermore, there is a similar apoptotic level of cloned embryos produced by the two types of cells. Finally, the full-term development ability of the cloned embryos was evaluated, and the cloned fetuses from the urine-derived cells showed absorption. Conclusions: Sow urine-derived cells could be used to produce SCNT embryos.