안면비대칭자의 3차원 전산단층사진 분석에서 경·연조직간 비대칭 정도 차이

김왕식,이기헌,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.3

본 연구는 안면비대칭 환자에서 경조직의 비대칭 정도와 연조직 비대칭 정도의 차이를 3차원적으로 밝히고자 시행되었다. 안면비대칭으로 보이는 성인 남녀 34명을 대상으로 두경부 전산단층사진을 촬영하고 3차원 입체영상으로 재구성한 후 기준평면에 대해 비대칭을 나타내는 6개의 계측항목을 경조직에 설정하고, 이에 대응하는 연조직계측항목을 각각 설정한 후 3차원 계측을 시행하고 경조직과 연조직의 계측항목간 차이를 비교하였다. 이부편위측과 반대측간의 계측치 차이를 비교한 결과, 경조직과 연조직 모두에서 좌우측 계측치관에 통계적으로 유의한 차이가 관찰되었으며 경조직과 연조직의 비대칭 계측항목을 비교한 결과. 0개의 계측항목 모두에서 통계적으로 유의한 차이를 나타내었다. 이부편위를 나타내는 chin deviation, 하악지와 하악골체를 나타내는 frontal ramal inclination difference. frontal corpus inclination difference 항목은 경조직의 비대칭 정도에 비하여 연조직 비대칭정도가 작게 나타난 반면, 입술경사를 나타내는 lip cheilion height difference. lip canting은 maxillary height difference, occlusal plane canting보다 크게 나타나 입술부위의 비대칭 정도는 하부 경조직의 비대칭정도보다 큰 것으로 나타났다. 안면비대칭자에서 경조직과 연조직간 비대칭 정도 차이를 규명한 본 연구 결과는안면비대칭 평가 시 경조직 외에 연조직 계측항목을 이용한 비대칭 분석도 필요함을 시사하였다. The purpose of this study was to compare the asymmetric degree between maxillofacial hard and soft tissues in individuals with facial asymmetry. Computerized tomographies (CT) of 34 adults (17 male, 17 female) who had facial asymmetry were taken. The CT images were transmitted to personal computers and then reconstructed into three-dimensional (3D) images through the use of computer software. In order to evaluate the degree of facial asymmetry, 6 measurements were constructed as the hard tissue measurements while 6 counterpart measurements were taken as the soft tissue measurements. The means and standard deviations were obtained for each measurement using 3D measure, then t-test was used to investigate the differences between each hard tissue measurement and the corresponding soft tissue measurement. All measurements used in the present study showed statistically significant differences between the hard and soft tissues. The degree of soft tissue asymmetry was smaller than that of corresponding hard tissue asymmetry in case of chin deviation, frontal ramal inclination difference, and frontal corpus inclination difference. On the other hand, the degree of soft tissue asymmetry was greater than that of underlying hard tissue asymmetry in the measurement of lip canting and lip cheilion height difference. The present study suggests that asymmetric differences of hard and soft tissue is observed in facial asymmetric subjects, and thus soft tissue analysis is needed in addition to hard tissue analysis when making an evaluation of facial asymmetry.

분만 전·후 사료급여 형태가 젖소의 생산성에 미치는 영향

기광석,김현섭,이왕식,이현준,김상범,정하연,은정식,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This experiment was carried out to protect drops of feed intake should be plural prepartum and postpartum to reduce metabolic diseases after calving to know how feeding systems, which is divided as a low quility, a high quility and total mixed rations(TMR), affects on postpartum productivity of Holistein cows. Three diets (low or high quality forage separately fed with concentrate and TMR containing high quality roughage) were fed to 21 cows from 3 weeks prepartum to 8 weeks postpartum to examine their effects on the productivity of cows. DM intakes was noticed significantly higher with TMR (17.11kg/day) than low- quality (13.48 kg/day) and high-quality forage (13.10kg/day). TDN and CP intakes were also higher with TMR compared to other experimental diets. Mean daily milk yield was non-significant among the cows fed different diets. Blood non-esterified fatty acids(NEFA) content was higher in cows fed low-quality or high-quality forage separately with concentrate compared with those fed TMR. The results concluded that TMR feeding to transitional cows is better than feeding the low or high quality forage separately for their health and productivity. 본 시험은 분만 전 3주부터 분만 후 3주까지 전환기라고 지칭되는 시기의 젖소들에 대한 대사적 변화를 최소화하기 위한 방법으로 사료 섭취량 저하를 방지하고 분만 후 대사성 질병의 발생을 줄이기 위한 목적으로 수행되어졌다. 대사성 질병에 관여하는 주요 요인으로는 건물섭취량, 혈중 glucose 함량, NEFA 함량, 반추위 환경, 면역 등인데, 분만 전·후 Holstein 젖소에 사료급여 형태를 조·농분리(저질 조사료와 양질 조사료 급여구) 및 TMR로 다르게 하였을 때 사료섭취량 변화와 혈중 NEFA 및 glucose 함량 변화를 측정하고 분만 후 젖소의 생산성에 미치는 영향을 구명하기 위하여 수행하였다. 처리별 사료건물 섭취량은 저질 조사료 조·농 분리, 양질조사료 조·농 분리, TMR 급여구에서 각각 13.48, 13.10, 17.11kg으로 TMR 급여구에서 유의성 있게 높았으며(P〈0.05), TDN 섭취량은 처리구별 각각 7.35, 7.19, 5.66kg으로 처리간에 차이가 없었다(P〉0.05). 신체충실지수(BCS)는 저질조사료구에서는 3.03이였으며, 양질 조사료구와 TMR 급여구에서는 각각 3.19, 3.16이었다. 분만후 1주부터 5주까지 측정한 평균 산유량은 저질조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 34.1, 35.1, 35.6kg 였으며, 분만 후 4∼5주에 최고 비유기에 달했다. 처리별 평균 유지율은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 3.74, 3.68, 3.95%였다. 혈청내 NEFA 함량은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 744.4, 381.8,266.7μEq/L로 처리간에 큰 차이를 보여 양질 조사료 급여와 TMR 급여시 혈중 NEFA 함량이 저질 조사료 급여에 이해 유의성 있게 낮았다(P〈0.05). 혈청내 glucose함량은 저질조사료 및 양질 조사료 조·농 분리, TMR 급여구에서 각각 39.3, 56.3, 62.4mg/dl로 양질 조사료 급여와 TMR 급여시 혈중 glucose 함량이 저질 조사료급여에 비해 유의성 있게 높았다(P〈0.05). 이상의 결과들을 종합해 볼 때, 분만 전 3주부터 분만 후 3주까지의 전환기 동안 사료섭취량 증가와 대사성 질병의 지표로서 혈중 NEFA 및glucose 함량 변화를 살펴 본 결과 분만 전 3주부터는 양질의 조사료나 TMR 형태로 급여하는 것이 사료섭취량 증가와 대사성 질병의 발생을 감소시킬 수 있을 것으로 사료되었다.

빈랑 추출물의 새로운 항바이러스 활성

이도승(Doseung Lee),부경환(Kyung Hwan Boo),김영천(Young Cheon Kim),이진만(Jin-Man Lee),강승태(Seungtae Kang),이왕식(Wang Shik Lee),류기중(Key Zung Riu),이동선(Dong-Sun Lee) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.2

Newcastle disease virus (NDV) 감염된 baby hamster kidney(BHK) 세포에서 합포체 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase (HN)에 의해 일어난다. HAU 값은 추출물의 농도가 25.0과 3.2 μg/mL 사이에서는 현저하게 감소하였으나, NDV 감염된 HAD (%)는 25.0 μg/mL 농도에서 광범위한 흡착능의 감소를 나타내 바이러스 당단백질의 세포내 생합성은 저해되지 않았다. 그러므로, 약용식물인 빈랑 메탄올 추출물이 바이러스 당단백질의 세포막으로의 수송과 함께 합포체 형성을 저해하여 항바이러스 작용을 하였다. 또한 빈랑 추출물의 저해활성을 조사한 결과 α-glucosidase에 대한 추출물의 IC<SUB>50</SUB>은 10.0 μg/mL이었으며, α-mannosidase, β-glucosidase, β-mannosidase에 대한 빈랑 추출물의 IC<SUB>50</SUB>은 각각 20.0, 40.0, 80.0 μg/mL로 나타나 β-type glycosidases 보다 α-type glycosidases에 대한 효소활성 저해능이 우수하였다. 따라서 IC<SUB>50</SUB>농도에서는 세포내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 판단되었으며 향후 항바이러스 관련 작용기작의 연구가 필요하다고 판단된다. Trafficking of viral glycoproteins to the cell surface results in syncytium formation in baby hamster kidney (BHK) cells infected with Newcastle disease virus (NDV). An extract from the medicinal Areca catechu L plant inhibited not only syncytium formation, but also trafficking of the hemagglutinin-neuramidase (HN) glycoprotein to the cell-surface. The viral glycoprotein was processed within the endoplasmic reticulum during transit to the cell membrane. Fungal extracts showed inhibitory activities (IC<SUB>50</SUB> 10 μg/mL) against α-glucosidase. These results suggested that A. catechu L. extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens

Zhang, Jiao Jiao,Wang, Xian Zhong,Kwon, Taeho,Huynh, Do Luong,Chandimali, Nisansala,Kim, Nameun,Kang, Tae Yoon,Ghosh, Mrinmoy,Gera, Meeta,Lee, Sang Baek,Lee, Sung Jin,Lee, Wang Shik,Kim, Seong Bong,Mo MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.8

<P>As an innovative technology in biological applications—non-thermal plasma technique—has recently been applied to living cells and tissues. However, it is unclear whether non-thermal plasma treatment can directly regulate the growth and development of livestock. In this study, we exposed four-day-incubated fertilized eggs to plasma at 11.7 kV for 2 min, which was found to be the optimal condition in respect of highest growth rate in chickens. Interestingly, plasma-treated male chickens conspicuously grew faster than females. Plasma treatment regulated the reactive oxygen species homeostasis by controlling the mitochondrial respiratory complex activity and up-regulating the antioxidant defense system. At the same time, growth metabolism was improved due to the increase of growth hormone and insulin-like growth factor 1 and their receptors expression, and the rise of thyroid hormones and adenosine triphosphate levels through the regulation of demethylation levels of growth and hormone biosynthesis-related genes in the skeletal muscles and thyroid glands. To our knowledge, this study was the first to evaluate the effects of a non-thermal plasma treatment on the growth rate of chickens. This safe strategy might be beneficial to the livestock industry.</P>

Innovative Design and Integrated Manufacturing Laboratory at Seoul National University

Sung-Hoon Ahn,Won-Shik Chu,Varsha Khare,Gil-Yong Lee,Wei Wang,Dong-Hyeon Kim,Sung-Ik Park,Min-Woo Han,Hyun-Taek Lee,Min-Cheol Kim,Jang-Yeob Lee,Hyung-Soo Kim,Min-Soo Kim,Kang-In Lee,Ying Jun Quan,Ji-H 한국생산제조학회 2016 한국생산제조시스템학회 학술발표대회 논문집 Vol.2016 No.10

초두구 추출물의 α-glucosidase 활성 억제에 의한 항바이러스 활성

이도승 ( Do Seung Lee ),부경환 ( Kyung Hwan Boo ),김영천 ( Young Cheon Kim ),이진만 ( Jin Man Lee ),김성철 ( Seong Cheol Kim ),이왕식 ( Wang Shik Lee ),류기중 ( Key Zung Riu ),이동선 ( Dong Sun Lee ) 한국응용생명화학회 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.2

In the present study, we investigated the effects of methanol extracts from Alpinia katsumadai Hayata against antiviral potential underlying mechanism in glucosidase inhibition. Syncytium formation in Newcastle disease virus (NDV)-infected baby hamster kidney (BHK) cell originates from the trafficking of viral glycoprotein into cell-surface. Methanol extracts inhibited not only syncytium formation, but also trafficking of glycoprotein, hemagglutinin-neuraminidase (HN), onto cell-surface. A. katsumadai extracts showed the inhibitory activities (IC50 25 μg/mL) against α-glucosidase. These results suggested that blue chanterelle extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

Intravascular Optical Molecular Imaging of a Macrophage Subset Within Intraplaque Hemorrhages

Lee, Jae Joong,Lee, Min Woo,Kim, Tae Shik,Song, Joon Woo,Nam, Hyeong Soo,Oh, Dong Joo,Oh, Wang-Yuhl,Yoo, Hongki,Park, Kyeongsoon,Kim, Jin Won Elsevier 2018 JACC. Cardiovascular imaging Vol.11 No.2

初生兒 呼吸困難症候의 臨床觀察

李賢淑,鄭求昌,王福鎭,陳東植 金原出版 1969 小兒科 Vol.10 No.6

Chronic HMGCR/HMG-CoA reductase inhibitor treatment contributes to dysglycemia by upregulating hepatic gluconeogenesis through autophagy induction

Wang, Hye Jin,Park, Jae Yeo,Kwon, Obin,Choe, Eun Yeong,Kim, Chul Hoon,Hur, Kyu Yeon,Lee, Myung-Shik,Yun, Mijin,Cha, Bong Soo,Kim, Young-Bum,Lee, Hyangkyu,Kang, Eun Seok Informa UK (TaylorFrancis) 2015 AUTOPHAGY Vol.11 No.11

<P>Statins (HMGCR/HMG-CoA reductase [3-hydroxy-3-methylglutaryl-CoA reductase] inhibitors) are widely used to lower blood cholesterol levels but have been shown to increase the risk of type 2 diabetes mellitus. However, the molecular mechanism underlying diabetogenic effects remains to be elucidated. Here we show that statins significantly increase the expression of key gluconeogenic enzymes (such as G6PC [glucose-6-phosphatase] and PCK1 (phosphoenolpyruvate carboxykinase 1 [soluble]) in vitro and in vivo and promote hepatic glucose output. Statin treatment activates autophagic flux in HepG2 cells. Acute suppression of autophagy with lysosome inhibitors in statin treated HepG2 cells reduced gluconeogenic enzymes expression and glucose output. Importantly, the ability of statins to increase gluconeogenesis was impaired when ATG7 was deficient and BECN1 was absent, suggesting that autophagy plays a critical role in the diabetogenic effects of statins. Moreover autophagic vacuoles and gluconeogenic genes expression in the liver of diet-induced obese mice were increased by statins, ultimately leading to elevated hepatic glucose production, hyperglycemia, and insulin resistance. Together, these data demonstrate that chronic statin therapy results in insulin resistance through the activation of hepatic gluconeogenesis, which is tightly coupled to hepatic autophagy. These data further contribute to a better understanding of the diabetogenic effects of stains in the context of insulin resistance.</P>

운동으로 유발되는 발적성 심실성빈맥에 관한 연구

이영우,김준수,송재관,이명묵,류왕성,최윤식,오병희 대한내과학회 1986 대한내과학회지 Vol.30 No.2

Exercise induced paroxysmal ventricular tachycardia is a rare condition and there has been little information on the electrophysiological mechanism or pharmacologic response of this arrhythmia, In this study, we investigated the electphysiological and pharmacologic characteristics of exercise-induced paroxymal ventricular tachycardia. The results are as follows: 1) The mean age of patients was 31 years. Four were males and two were females. 2) Underlying heart diseases were dilated cardiomyopathy in 2 cases, Ebstein`s anomaly in 1case, atrial septal defect in 1 caee, and nothing in 2 cases. 3) Common subjective symptoms during ventricular tachycardia were palpitation, dyspnea and syncope. 5) Electrocardiograms during attack showed left bundle branch block in all cases. Right axis deviation was noted in 3 cases, suggesting the origin of ventricular tachycardias in the right ventricular outflow tract. 5) During treadmil exercise test, ventricular tachycardia occurred at stage I in one case, at stage II in one case, at stage g in two cases, and at post-exereise 1 minute in two cases. 6) Electrophysiological studies were done in 4 cases. Ventricular tachycardia was induced with incremental atrial pacing, ventricular extrastimuli and rapid ventricular pacing in one case, with rapid ventricular pacing during isoproterenol infusion(2㎍/minute) in one case, and not inducible in two CRSCB. 7) Reta-blocker was most effective to prevent the attack of exercise-induced VT. Also verapamil was seemed to be effective.