http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
How do Ribosomes Select the Initiation Codon ?
Sohn, Uik,M C Genoza G An 한국유전학회 1988 Genes & Genomics Vol.10 No.4
We have proposed a model for translational initiation in which interactions between tRNAi and mRNA may underlie much of the specificity of initiation(1). To test this hypothesis, we have constructed recombinant plasmids and mutants of these plasmids by manipulating the prestart region of β-galactosidase that can be expressed in both prokaryotic and eukaryotic cells.
Splice Signals in T - Cell Receptor Genes
Sohn, Uik,Kim, N,Ha, J 한국유전학회 1988 Genes & Genomics Vol.10 No.4
The Study of T-cell receptor (TCR) genes can provide clinically important information. For example, the rearrangement of TCR genes can be used as a useful marker of clonality in T-cell proliferative disorders and, as a target for immune therapy (neoplasias and autoimmune diseases). Our approach to address these issues has been to determine the structure and polymorphism of TCR α, β and γ-chain genes. Based on the analysis of splice junctions of these genes, we found extensive base-pairing between the 5′splice site (ss) sequence and U1. However, the branch site sequence, TACTAAC, and base-pairing between the 3′ss sequence and U5 have not been found.
Exon/intron Sequence of the Murine T Cell Receptor Constant (Cβ1) Gene
Sohn, Uik 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Germline DNA clones containing the constant genes (Cβ1 and Cβ2) coding for the β chain of the mouse T-cell receptor were isolated and nucleotide sequence of the Cβ1 gene was determined. The two constant (C)-region gene segments are almost identical over their entire coding region and splice junction sequences. Comparison of the mouse Cβ1 gene with the human counterpart reveals more than 80% sequence homologies in their coding regions and exon/intron boundaries. Both genes are arranged in four exons, interrupted by introns at the same positions.
손우익,이상숙,김기권,박준식,진승원 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Verrucous carcinoma of the larynx is a distinct and uncommon variant of well-differentiated squamous cellcarcinoma. The authors hybridized in situ a case of laryngeal verrucous carcinomma with DNA probes to human papillomavirus (HPV) 6, 11, 16, 18, 31, 33 and 35. The DNAs from the verrucous carcinoma of larynx hybridized with mixed DNA probes HPV-16/18 and 31/33/35. In addition, there was an evidence of HPV infection based on hybridization with mixed DNA probe HPV-6/11 in the adjacent papilloma tissue. By in situ DNA hybridization techniques, we clearly demonstrated human papillomavirus (HPV-16/18, and 31/33/35) -related sequences in this neoplasm. These findings suggest the role of HPV-6/11 in the development of laryngeal papilloma and HPV-16/18 and 31/33/35 probably on the progression to verrucous carcinoma.
자궁경부의 상피내암 조직내 In Situ Hybridization 에 의한 Human Papillomavirus DNA 의 검색 및 Typing
손우익,이상숙,이태성,김기권,정재홍,김택훈,진승원 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
The authors examined 12 condylomas, 55 cervital intraepithelial neoplasms(CIN) for the presence of human papillomavirus(HPV) DNA sequences by DNA-DNA in situ hybridization and reviewed the epithelial distribution and incidence of HPV DNA sequences in different cervical lesions. In situ hybridization to HPV DNA probes revealed target HPV DNA sequences mostly in the nuclei of the superficial cells from epithelium which contained either maturation of koilocytotic atypias, although in several cases hybridizing sequences were detected in the entire thickness of epithelium, including basal layer. The distribution of HPV was mostly focal and multicentric. With the use of biotinylated HPV DNA probes 6/11, 16/18 and 31/33/35, 41 of 55(74.5%) cases of CIN was positive with any HPV DNA probes, 20 of which were positive with HPV 6/11, 28 with HPV 16/18, 36 with HPV 31/33/35 and 27 with two or more mixed probes. HPV 31/33/35 was the most prevalent in CIN grade Ⅲ, followed by HPV 16/18, although there was no difference in the frequency of HPV types among condylomas, CIN grade Ⅰ and CIN grade Ⅱ. Cases of CIN under the age of 40 revealed higher HPV-positive rate and more abundant HPV DNA than those with age 40 or more. Mixed HPV infection, positive with 2 or more kinds of HPV DNA probes was found about half of the cases studied. Among type of condylomas, flat condyloma was the most frequently accompanied by CIN.
자궁상피내암종 및 침윤성 편평상피암에서의 In Situ Hybridization 에 의한 Human Papillomavirus DNA 의 검색
손우익,진승원,이상숙,김기권,정재홍 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
The authors examined 9 condylomas, 26 cervical intraepithelial neoplasms(CIN) and 22 invasive squamous cell carcinomas for the presence of human papillomavirus(HPV) DNA sequences by DNA-DNA in situ hybridization. In situ hybridization revealed target HPV DNA sequences mostly in the nuclei of the superficial cells from epithelium which contained either maturation or koilocytotic atypias. With the use of biotinylated HPV DNA probes 6/11, 16/18 and 31/33/35, 42 of the 57(73.7%) were positive with any HPV DNA mixed probes, 13 of which were positive with HPV-6/11, 23 with HPV-16/18, 32 with HPV-31/33/35 and 18 with two or more mixed probes. HPV-31/33/35 was the most prevalent in CIN and invasive squamous cell carcinomas, followed by HPV-16/18. The incidence of HPV DNA increased from 66.7% to 86.496 with increasing severity of the lesions from condylomas to invasive squamous cell carcinomas. Flat condyloma was most frequently accompanied by CIN.
HPV (Human papillomavirus) 양성인 자궁경부암에서의 세포성 면역 반응
손우익,이상숙,이태성,진승원 경북대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.7 No.1
To study the human immune response to HPV, three monoclonal antibodies against T cells(UCHL), B cells(L-26), and macrophages (CD68) were used in an immunohistochemical study of HPV-infected cervical cancer tissues. Most infilterlating leucocytes were found to be predominantly T cells and macrophages, with few B cells in all tissues examined. These results support the hyopothesis that cell-mediated immunity is related to the HPV-induced disease.