Comparison of Embryo Production Performance and Conception Rate after Embryo Transfer between Mongolian Cattle and Korean Native Cattle

Gantugs Chuluundorj,윤종택,이호준,손동수,Enkhmanlai Ganbaatar,Baldan Tumur 사단법인 한국동물생명공학회 2017 한국동물생명공학회지 Vol.32 No.4

This study is to compare the effect of estrus synchronization and embryo transfer between Korean and Mongolian cattle. Embryos were collected from 9 donors housed in Asan city in South Chungcheong Province, South Korea. Embryos were collected 9 donors from Khushaat sum, Selenge province and Bayanchandmani sum, Tov province in Mongolia. Follicle Stimulating Hormone (FSH), Controlled Internal Drug Release (CIDR) and Prostaglandin (PG) were used for superovulation. Subsequently, Artificial Insemination (AI) was done for donor cow and embryo was collected after 7 and 8 days. Collected embryos were compared between Mongolian and Korean cattle. Finally, good quality and fresh embryos were transferred to 50 and 22 recipients of cows in Korea and Mongolia respectively. The findings show that Korean native cattle each donor cow produced on an average 16.9 embryos and, 10.9 embryos were found transferable. But in case of Mongolia the average production of embryos per donor cow was 8.6 embryos and, 6.2 embryos were found transferable. Embryo collection after 7 and 8 days was not difference in embryo production in Korea. But, in Mongolia embryo production after 8 days was found more efficient than the 7 days. Korean native recipient's cows (74.6%) and Mongolian recipient's cows (71.0%) respectively were found transferable ovarian stage. The result suggested that efficiency of embryo production from the superovulation method treated of Korean cow were higher than the Mongolian cow. The pregnancy rate of Korea native cattle was 72%, which was about 10% higher than that of Mongolia cattle.

몽골 낙농산업 발전을 위한 인공수정 및 수정란 이식사업

권태현,최병현,조수진,Munkhbayar Tsolmon,Tumur Baldan,Naidansuren Durevjargal,공일근 한국동물생명공학회(구 한국동물번식학회) 2009 발생공학 국제심포지엄 및 학술대회 Vol.2009 No.1

Effect of Sex-sorted Semen in Superovulated Hanwoo

Enkhbolor Barsuren,Sang-Hwan Kim,Ho-Jun Lee,Baldan Tumur,Jong-Taek Yoon 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

Sex-sorted sperm is now widely used in cattle breeding to enhance industrial competitiveness and achieve high female fertility. We bought a "Whole-mom" from the Nuri Science Co., Ltd company. The objective of this study was to evaluate the pregnancy rates and calves sexing proportion of male and female calves produced using Artificial insemination (AI), both performed using sexed and conventional sperm. The study was carried out spring to autumn season on 150 Hanwoo bred on a farm. In all superovulated donors, inseminations were initiated 12 hours after the onset of standing estrus. In a experiment, to take a sexed semen was mixed with "Whole mom" and conventional semen in a water bath at 37℃ for 20 minutes. The sexed and nonsexed semen was deposited in the body of the uterus. After collection, embryo morphology was assessed under a stereomicroscope. Embryos were transferred to the uterine horn ipsilateral to the CL. The conception rates were 73.3% (45/33) sexed semen and 48.8% (90/44) conventional semen. The sex ratio for sexed-semen inseminations was 70% (21/30) females for singleton births within a 279.2 to 282.1 day gestation interval. The sex ratio for unsorted semen was 56.8% (25/44) females for births. The use of X-sorted sperm, already widely employed for AI in dairy cattle has the potential to alter the structure of the industry by increasing the replacement heifer supply, creating opportunities for using a proportion of the dairy herd for producing beef animals, and improving the rate of genetic selection.

Artificial Insemination and Embryo Transfer Project to Foster Mongolia Dairy Industry

Tae-Hyeon Kwon,Naidansuren Durevjargal,민찬식,공일근,Byeong-Hyun Choi,Su-Jin Cho,Munkhbatar Tsolmon,Tumur Baldan 사단법인 한국동물생명공학회 2009 한국동물생명공학회지 Vol.24 No.4

Mongolia has 80% livestock of total agriculture industry, 170,000 farms are engaged, 2,500,000 of cows that were beef and dairy cows are raised. Despite of Mongolian has great application with milk, there are not clear differences between cow and dairy cattle, and the production of milk is also low. But the milk suppliers are varied (horse, sheep, goat, etc), so that the total milk production is 500 thousand ton per year. It's really considerable to improve the breed of owing to many problems with big differences among milk qualities. For carrying out for first year project, artificial insemination project was operated with 3rd grade Holstein semen that were imported from S. Korea, and initiation and field training were also carried out through appropriate AI technique we developed for Mongolia environment. Local information research and MOU conclusion were done with professor D. Altangerel in May 10th~13th, 2009, and development for AI technique and AI equipments were supplied for Mongolia breeding and natural environment in July 10th~17th in 2009. All cows were treated by synchronization for AI. To do this, PGF2α injection were treated for luteal phase cow, if it wouldn't work, try again after 11 days. After confirmation of estrus, AI and AI training were carried out with sperm injection in the uterus or cervix by rectum-vagina method which is common worldwide, the most effective artificial insemination technique. If cows were return to next estrus cycle, second AI was carried out about approximately 21 days after artificial insemination. After 2 months, all cows not showing return estrus should be taken pregnancy test. Every pregnant cow will be cared thoroughly. Total 48 cows administrated by PGF2α for synchronization and after 48 hours 45 cows (93.8%) showing estrus were detected and then artificial inseminate them within who 8 cows (27.8%) showed return estrus. Therefore, Using PGF2α for synchronization is effective to use for Mongolia breeding conditions. There are possibility of base for food production after all, including increase of livestock production in Mongolia by improvement of breeding cow with AI and embryo transfer project.

Artificial Insemination and Embryo Transfer Project to Foster Mongolia Dairy Industry

Kwon, Tae-Hyeon,Choi, Byeong-Hyun,Cho, Su-Jin,Tsolmon, Munkhbatar,Durevjargal, Naidansuren,Baldan, Tumur,Min, Chan-Sik,Kong, Il-Keun 韓國受精卵移植學會 2009 한국동물생명공학회지 Vol.24 No.4

Mongolia has 80% livestock of total agriculture industry, 170,000 farms are engaged, 2,500,000 of cows that were beef and dairy cows are raised. Despite of Mongolian has great application with milk, there are not clear differences between cow and dairy cattle, and the production of milk is also low. But the milk suppliers are varied (horse, sheep, goat, etc), so that the total milk production is 500 thousand ton per year. It's really considerable to improve the breed of owing to many problems with big differences among milk qualities. For carrying out for first year project, artificial insemination project was operated with 3rd grade Holstein semen that were imported from S. Korea, and initiation and field training were also carried out through appropriate AI technique we developed for Mongolia environment. Local information research and MOU conclusion were done with professor D. Altangerel in May , 2009, and development for AI technique and AI equipments were supplied for Mongolia breeding and natural environment in July in 2009. All cows were treated by synchronization for AI. To do this, injection were treated for luteal phase cow, if it wouldn't work, try again after 11 days. After confirmation of estrus, AI and AI training were carried out with sperm injection in the uterus or cervix by rectum-vagina method which is common worldwide, the most effective artificial insemination technique. If cows were return to next estrus cycle, second AI was carried out about approximately 21 days after artificial insemination. After 2 months, all cows not showing return estrus should be taken pregnancy test. Every pregnant cow will be cared thoroughly. Total 48 cows administrated by for synchronization and after 48 hours 45 cows (93.8%) showing estrus were detected and then artificial inseminate them within who 8 cows (27.8%) showed return estrus. Therefore, Using for synchronization is effective to use for Mongolia breeding conditions. There are possibility of base for food production after all, including increase of livestock production in Mongolia by improvement of breeding cow with AI and embryo transfer project.