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Molecular Identification of Microbial Community in Chinese Douchi During Post-fermentation Process
Tingtao Chen,Shunqiang Xiong,Shuying Jiang,Mengjuan Wang,Qinglong Wu,Hua Wei 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6
To track changes in dynamic microbial communities during post-fermentation process, traditional culture method, and denaturing gradient gel electrophoresis (DGGE) were used to study the number and species of dominant microorganisms in douchi. The result of culturedependent method showed that the microbial number changed slightly since the 4^th day while the DGGE indicated that the really steady-state was achieved from the 10^th day. In addition, Lactococcus lactis subsp.,Staphylococcus lentus, and 2 uncultured bacterium were identified to occupy the dominant positions in bacterial DGGE pattern, and Bacillus thermoamylovorans, Bacillus subtilis, Enterobacter spp., and Absidia corymbifera,Pichia guilliermondii, Pichia farinose were also detected from Bacillus and fungal DGGE patterns, respectively. In conclusion, some pathogenic microorganisms involving in the douchi fermentation had been detected throughout the post-fermentation process, and the combination of culturedependent and –independent method was proved to be effective in profiling microbial diversity.
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Di Xu,Xiaoli Wu,Bo Li,Peng Li,Xing Ming,Tingtao Chen,Hua Wei,Feng Xu 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
Campylobacter jejuni is a worldwide foodborne pathogen recognized as a leading cause of human gastrointestinal enteritis. A rapid, sensitive, and specific method is required to monitor food and water in cases of contamination by this pathogen. This report presents a novel immunochromatographic test (ICT) using fluorescent microspheres labeled with polyclonal antibodies of C. jejuni as the capture reagent dispensed onto the conjugate pad. Polyclonal antibodies against the outer membrane protein PEB1 of C. jejuni were used as the detective reagent at the test line, whereas the goat anti-rabbit IgG was used on the control line. PEB1 was obtained by gene cloning and expression to prepare its antibody. In this study, a simple and rapid ICT is reported for detecting C. jejuni for the first time with a detection limit of 106 CFU/mL.
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