Finance and Corporate Innovation: A Survey

Xuan Tian,Jie (Jack) He 한국증권학회 2018 Asia-Pacific Journal of Financial Studies Vol.47 No.2

Corporate innovation is an increasingly important topic that has attracted great attention from academic researchers in financial economics in recent years. Although the top three finance journals (i.e. the Journal of Finance, the Journal of Financial Economics, and the Review of Financial Studies) together published a total of only five papers on corporate innovation from 2000 to 2008, the number of such papers published by these three journals skyrocketed to 56 from 2009 to the third quarter of 2017. The purpose of this survey is to provide a synthetic and evaluative monograph of academic papers that examine the drivers and financing sources of corporate innovation.

Synthesis of novel oleanolic acid and ursolic acid in C-28 position derivatives as potential anticancer agents

Tian Tian,Xinyu Liu,이응석,Jingyang Sun,Zhonghua Feng,Long-Xuan Zhao,Chunhui Zhao 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.4

A series of nitrogen-containing derivatives ofoleanolic acid and ursolic acid were prepared by a modificationat C-28 position via esterification with 2-hydroxyaceticacid followed by amidation with amines, such aspiperazine, N-methylpiperazine, and alkane-1, 2-diamines,alkane-1, 4-diamines, alkane-1, 6-diamines. In vitroantiproliferative activities of the compounds preparedtowards MCF-7, Hela and A549 cell lines were evaluatedby a MTT method to show that OA-5a, OA-5b, OA-5c andUA-5a showed somewhat improved antiproliferativeactivities against MCF-7, Hela and A549 cells comparingto that of the positive control, gefitinib.

Biocatalysis and Bioprocess Engineering : Overproduction of the Escherichia coli Chaperones GroEL-GroES in Rhodococcus ruber Improves the Activity and Stability of Cell Catalysts Harboring a Nitrile Hydratase

( Yu Xuan Tian ),( Jie Chen ),( Hui Min Yu ),( Zhong Yao Shen ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2

Three combinations of molecular chaperones from Escherichia coli (i.e., DnaK-DnaJ-GrpEGroEL- GroES, GroEL-GroES, and DnaK-DnaJ-GrpE) were overproduced in E. coli BL21, and their in vitro stabilizing effects on a nitrile hydratase (NHase) were assessed. The optimal gene combination, E. coli groEL-groES (ecgroEL-ES), was introduced into Rhodococcus ruber TH3. A novel engineered strain, R. ruber TH3G was constructed with the native NHase gene on its chromosome and the heterologous ecgroEL-ES genes in a shuttle plasmid. In R. ruber TH3G, NHase activity was enhanced 37.3% compared with the control, TH3. The in vivo stabilizing effect of ecGroEL-ES on the NHase was assessed using both acrylamide immersion and heat shock experiments. The inactivation behavior of the in vivo NHase after immersion in a solution of dynamically increased concentrations of acrylamide was particularly evident. When the acrylamide concentration was increased to 500 g/l (50%), the remaining NHase activity in TH3G was 38%, but in TH3, activity was reduced to 10%. Reactivation of the in vivo NHases after varying degrees of inactivation was further assessed. The activity of the reactivated NHase was more than 2-fold greater in TH3G than in TH3. The hydration synthesis of acrylamide catalyzed by the in vivo NHase was performed with continuous acrylonitrile feeding. The final concentration of acrylamide was 640 g/l when catalyzed by TH3G, compared with 490 g/l acrylamide by TH3. This study is the first to show that the chaperones ecGroEL-ES work well in Rhodococcus and simultaneously possess protein-folding assistance functions and the ability to stabilize and reactivate the native NHases.

Synergistic effect of micro-textures and MoS2 on the tribological properties of PTFE film against GCr15 bearing steel

Xuan Xie,Xijun Hua,Jianhua Li,Xiaobin Cao,Zhixiang Tian,Rui Peng,Bifeng Yin,Peiyun Zhang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5

To enhance the friction and wear resistance of GCr15/PTFE sliding contact surfaces, the synergistic lubrication effect of micro-textures and solid lubricants were studied in this paper. Micro-textures were processed on GCr15 samples by Nd:YAG laser, which were filled with MoS 2 . Tribological performance tests were carried out on a Rtec-MFT 5000 tester. Tribological properties were optimal with the textures density of 20 % and depth of 9 μm. A continuous composite solid lubricating film was formed by PTFE and MoS 2 . As load increases, friction coefficient of the sample shows a rapid decline and then stabilizes. With the increase of reciprocating frequency, friction curve of the sample shows a trend of slight fluctuation. When frequency is 2.7 Hz, friction coefficient is the lowest. Tribological properties of PTFE/GCr15 contact surfaces could be significantly optimized by micro-textures and MoS 2 , which performs favorable prospect of engineering application such as guide rails, bearings, machinery seal, etc.

LINC01272 Suppressed Cell Multiplication and Induced Apoptosis Via Regulating MiR-7-5p/CRLS1 Axis in Lung Cancer

( Xuan Ma ),( Yang Liu ),( Hao Tian ),( Bo Zhang ),( Meiling Wang ),( Xia Gao ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.7

LINC01272 is a long non-coding RNA (lncRNA) that has been considered as a biomarker for many diseases including lung squamous cell carcinoma. Here, we investigated the function and mechanism of LINC01272 on lung cancer (LC). The differential expression of LINC01272 in LC and normal samples was analyzed by GEPIA based on the data from TCGA-LUAD database, as survival prognosis was analyzed through Kaplan-Meier Plotter. LINC01272 overexpression plasmid and miR-7-5p mimic were transfected into A549 and PC-9 cells. LINC01272, miR-7-5p and cardiolipin synthase 1 (CRLS1) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction. Cell viability was detected through MTT assay. Cell multiplication was evaluated by cell formation assay. Cell apoptosis was assessed through flow cytometry assay. Through bioinformatics, the target miRNA of LINC01272 and downstream genes of miR-7-5p were predicted. The targeting relationship was tested by dual luciferase reporter analysis. CRLS1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax) and cleaved caspase-3 protein levels were detected through western blot. LINC01272 was downregulated in LC and low LINC01272 expression had poor prognosis. In A549 and PC-9 cells, LINC01272 inhibited cell viability and multiplication and induced apoptosis. LINC01272 negatively regulated miR-7-5p and CRLS1 was a target of miR-7-5p. MiR-7-5p reversed the effect of LINC01272 on viability, multiplication, apoptosis and expression of miR-7-5p and CRLS1 as well as apoptosis-related factors (Bcl-2, Bax and cleaved caspase-3). LINC01272 suppressed cell multiplication and induced apoptosis via regulating the miR-7-5p/CRLS1 axis in LC.

P3H4 promotes renal cell carcinoma progression and suppresses antitumor immunity via regulating GDF15-MMP9-PD-L1 axis

Tian, Shuo,Huang, Yan,Lai, Dong,Wang, Hanfeng,Du, Songliang,Shen, Donglai,Chen, Weihao,Xuan, Yundong,Lu, Yongliang,Feng, Huayi,Zhang, Xiangyi,Zhao, Wenlei,Wang, Chenfeng,Wang, Tao,Wu, Shengpan,Huang, Techno-Press 2022 Advances in nano research Vol.12 No.6

The prolyl 3-hydroxylase family member 4 (P3H4), is associated with post-translational modification of fibrillar collagens and aberrantly activated in cancer leading to tumor progression. However, its role in clear cell renal cell carcinoma (ccRCC) is still unknown. Here we reported that P3H4 was highly expressed in renal cancer tissues and significantly positive correlated with poor prognosis. Knockdown of P3H4 inhibited the proliferation, migration and metastasis of renal cancer cells in vitro and in vivo, and also, overexpression of it enhanced the oncogenic process. Mechanistically, P3H4 depletion decreased the levels of GDF15-MMP9 axis and repressed its downstream signaling. Further functional studies revealed that inhibition of GDF15 suppressed renal cancer cell growth and GDF15 recombinant human protein (rhGDF15) supplementation effectively rescued the inhibitory effect induced by P3H4 knockdown. Moreover, decreased levels of MMP9 caused by inhibition of P3H4-GDF15 signaling constrained the expression of PD-L1 and suppression of P3H4 accordingly promoted anti-tumor immunity via stimulating the infiltration of CD4+ and CD8+ T cells in syngeneic mice model. Taken together, our findings firstly demonstrated that P3H4 promotes ccRCC progression by activating GDF15-MMP9-PD-L1 axis and targeting P3H4-GDF15-MMP9 signaling pathway can be a novel strategy of controlling ccRCC malignancy.

EMI Modeling and Experiment of a GaN Based LLC Half-Bridge Converter

Mofan Tian,Yuan Hao,Kangping Wang,Yang Xuan,Lang Huang,Jingjing Sun,Xu Yang 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

This paper focuses on the electromagnetic interference (EMI) research and analysis of the MHz switching frequency GaN MOSFET based on the LLC resonant DC-DC converter. In this paper, first, the CM coupling paths are studied to get simplified models. Then the impact of the parasitic capacitors (both the capacitors to the ground and the capacitors in the devices) on the CM current are analyzed. Finally the high frequency model is derived from the work above. Moreover this paper makes the comparison between normal Si MOSFET and GaNbased MOSFET behavior in EMI. The modeling result proves that GaN MOSFET has a worse behavior on EMI test. An experiment layout which is aimed at minimizing the irrelevant factors is designed and the experiment result proves the accuracy of the modeling.

Establishment of a novel myocarditis mouse model based on cyclosporine A

Zhao Tian Hao,Jiang Yi Xuan,Chen Kai Qin,Qiu Dan,Xu Yan Zhe,Ye Chun,Ren Ting,Zhang Bo,Dai Bin,Hu Jue,Lu Jun,Zhou Fang Liang,Xiao Rong,Lu Fang Guo,Wei Ke 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Myocarditis is a myocardial injury that can easily cause adolescent death. Traditional research models of animal invasion with viral components, lipopolysaccharide (LPS) or porcine myocardial myosin, among others, have the shortcomings of potential biological safety hazards and high animal mortality. Objective: To explore the construction of a novel myocarditis model with cyclosporine A and the potential genes and pathways associated with it. Methods: BALB/c mice were used in this study, and cyclosporin A and LPS were injected into the peritoneal cavity of mice. The successful establishment of the model was assessed by detecting serum myocardial injury markers and inflammatory factors levels, HE, IHC staining, and RT-qPCR methods. Key genes were obtained using the GSE35182 dataset from the GEO database and validated with the RT-qPCR method. Results: We found that a large number of inflammatory cells infiltrated the myocardium of mice in each group of Cyclosporin A constructed model, while the expression of inflammatory factor indicators was increased, and this model has the characteristics of high degree of local inflammation in myocardial tissue, low mortality, and safe and non-toxic treatment. Using GSE35182 data, we selected 18 Hub genes and validated Hub genes in myocardial tissue with RT-qPCR and found that multiple signaling pathways such as Toll-likereceptor signaling pathway(TLRs), Rap1 signal pathway(Rap1), and Chemokine signaling pathway may be involved in the development of myocarditis. Conclusion: Cyclosporin A can construct a new myocarditis model, and TLRs, Chemokines and Rap1 signaling pathways may be the core pathways of myocarditis.

Design and Implementation of a High Power Three-Level T-Type Inverter for a Photovoltaic System

Yang Xuan,Mofan Tian,Xiaomei Song,Wenjie Chen,Xu Yang 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Nowadays, the grid-connected photovoltaic systems are an important part of the renewable energy sources, and their performance is getting more and more important. Many reported researches focused on the traditional NPC topology. However, few researches were about the T-type NPC inverter. In this paper, a high power three-phase T-type neutral point clamped grid connected photovoltaic inverter prototype is designed and implemented. The design and implementation procedure of the inverter is discussed in detail. Experimental results showed that the output power of the proposed inverter could reach up to 100kW with unit power factor, while the total harmonic distortion of the grid current was less than 3%. And the total efficiency of the system is measured as 95.6%. The experimental results demonstrated the T-type NPC inverter can operate with a good performance.

Significance of Thrombocytosis in Clinicopathologic Characteristics and Prognosis of Gastric Cancer

Li, Fang-Xuan,Wei, Li-Juan,Zhang, Huan,Li, Shi-Xia,Liu, Jun-Tian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Purpose: We aimed to study the relationship between thrombocytosis and clinical features of gastric cancerfocussing on platelet counts and gastric cancer progression through different TNM stages. Methods: According to the normal range of platelet count in our institution, 1,596 patients were divided to two groups: a thrombocytosis group (120 patients, > $400{\times}1000/{\mu}L$) and a control group (1,476 patients, ${\leq}400{\times}1000/{\mu}L$). Results: The incidence of thrombocytosis was 7.5%. Higher platelet counts were observed in patients with older age, larger tumor size, deeper invasion, lymph node metastasis, distant metastasis and advanced TNM stage. In multivariate logistic regression, tumor size, depth of tumor invasion, lymph node metastasis and TNM stage were independent risk factors for thrombocytosis of gastric cancer patients. On prognostic analysis, age, tumor size, tumor location, histologic type, depth of tumor invasion, lymph node metastasis, distant metastasis and TNM stage and platelet count were important factors. Tumor size, invasion depth, lymph node metastasis, TNM stage and the platelet count were independent prognostic factors. Conclusion: Thrombocytosis is associated with clinical features of gastric cancer patients and correlates with a poor prognosis.