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Fabrication of microfluidic PDMS chips to design noble patterns
Thapa Mukesh,허윤석 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.4
Fabricated microfluidic chips have a great implication in present because of their durability, miniature size, computational accessibility, and control. However, all these application developments are still restraining because of surface limitation. Development and validation of a good wave-patterned topology and grooves for cellular attachment would improve the efficiency of basic cell culture on Polydimethylsiloxane-microfluidic chips. A short length PDMS-PDMS chip with six long obstacles were prepared and different combinational (flowrate, time, concentration) TBAF based wet etching was conducted. Were as a secondary concave wafer layer over a rectangular cavity chip were prepared to formulate concave chips. The overall etching-rate in our designs for completion of reaction was relative with previously conducted etching parameters. variation of the model also provided positive results. PDMS platform- Etching method generates a good sample culturable wave pattern for provided channel to facilitate possible biological cell culture and diagnosis platform. We generate a proper cell culturable wave pattern with crisp semi-circular-groove using wet etching and a concave 3-dimensional cavity base chip with help of PDMS secondary wafer system.
확장/축소 배열의 마이크로 채널의 관성력을 이용한 적혈구와 순환 종양 세포 분리 연구
이성한(Sung Han Lee),Thapa Mukesh,박민규(Min Gyu Park),채혜송(Hyeon Song Chae),허윤석(Y.S. Heo) 대한기계학회 2018 대한기계학회 춘추학술대회 Vol.2018 No.12
Recently, particle separation studies have been conducted in micro systems. Particularly, the method of separating particles by a hydrodynamic method requires no additional external force. It is also possible to separate cells from microfluidics systems using cells instead of particles. We have conducted a study to isolate circulating tumor cells from red blood cells. Because circulating tumor cells are present in very small amounts compared to blood cells, the enrichment step is required for effective detection. We designed expansion/contraction array microchannel, which separates circulating tumor cells(MCF-7) from red blood cells by inertial force. This microchannels have experimentally proven that cells can be separated with high throughput and high efficiency.