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Sharma, G. Taru,Teotia, Alok,Majumdar, A.C. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.6
Evaluation of the granulosa cell (GC) monolayers developed from small (<5 mm) and large (>5 mm) follicles on the meiotic competence of caprine oocytes during in vitro maturation was done in this study in comparison to the granulosa cell coculture. Ovaries were collected from the local abattoir and follicular contents were aspirated for the monolayer culture. For IVM the oocytes were collected by puncturing the nonatretic follicles (>4 mm). Results revealed that at the same seeding rate, small follicular granulosa cell monolayer achieved confluence 24-48 h earlier than large follicular granulosa cell monolayer. GC monolayers significantly p (<0.05) improved the rate of meiotic resumption and nuclear maturation (84.76% vs 74.74%) after 27 h of culture in comparison to GC coculture. Statistically there was no significant difference in the maturation rate between the caprine oocytes matured over small or large follicular GC monolayers. It is concluded from the present study that GC monolayers support better nuclear and cytoplasmic maturation of growing caprine oocytes which is evident by better maturation rate over GC monolayer as compared to the oocytes matured with GC coculture. Granulosa cells from small and large follicles can be used for IVM with more or less in the same efficiency after conditioning them with maturation media in 18-24 h before the onset of culture.
Hussein, Kamal H.,Park, Kyung M.,Teotia, Pankaj K.,Hong, Seok H.,Yang, Se R.,Park, Sung M.,Ahn, Cheol,Woo, Heung M. SAGE Publications 2013 The International journal of artificial organs Vol.36 No.11
<P>The aims of this study were to investigate the effects of sterilization with peracetic acid (PAA) and ethanol on the biological activity of porcine liver scaffolds and to develop a new technique for sterilization using slightly acidic electrolyzed water (SAEW).</P>
Kidney injury molecule-1 is involved in the chemotactic migration of mesenchymal stem cells.
Park, Kyung-Mee,Nam, Hyun-Suk,Teotia, Pankaj Kumar,Hussein, Kamal Hany,Hong, Seok-Ho,Yun, Jung-Im,Woo, Heung-Myong Springer 2014 In vitro cellular & developmental biology Animal Vol.50 No.7
<P>A better understanding of the organ specific factors that regulate the migration of mesenchymal stem cells (MSCs) into the target organ is essential for optimization of strategies to improve the repair after injury. In the present study, we showed that the kidney injury molecule-1 (KIM-1), a well-known kidney-specific biomarker, enhanced the in vitro migration capacity of MSCs as a potent kidney-specific chemo-attractant or an inducer. The in vitro roles were verified by migration assay using KIM1-PK1 cell lines, the mouse proximal tubular epithelial cells (mPTEs) and recombinant human KIM-1 proteins (rhKIM-1). Immunofluorescence staining displayed specific ectodomain binding of KIM-1 on the surface of MSCs. Upregulation of chemokine receptor type 4 (CXCR4) protein when treated with tumor necrosis factor alpha (TNF-α) was shown. The effect of KIM-1 on migration of MSCs was augmented by TNF-α pretreatment in a dose-dependent manner, and reduced by AMD3100, an antagonist of CXCR4. These results suggest that KIM-1 is a potential chemo-ligand of CXCR4 and may play an important role in kidney-specific migration of MSCs via interaction between KIM-1 and CXCR4.</P>
Construction of a Biocompatible Decellularized Porcine Hepatic Lobe for Liver Bioengineering
Hussein, Kamal H.,Park, Kyung M.,Kim, Hyun M.,Teotia, Pankaj K.,Ghim, Jinn H.,Woo, Heung M. SAGE Publications 2015 The International journal of artificial organs Vol.38 No.2
<P>One of the major obstacles in applying decellularized organs for clinical use is the recellularization step, during which huge numbers of cells are required to develop whole livers. We established a simple protocol for constructing a bioartificial hepatic lobe and investigated its biocompatibility.</P>