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      저자 : Tania S. Das (검색결과 1 건)
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        In vivo and invitro experimentation for scientific validation of the traditional use of Chromolaena odorata (L.) against envenomation of honey bee sting

        Tania S. Das,Biswadeep B. Das,Chandrasekhar Mote 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.1

        Honey bees are well known for their economic importance but despite of their domicile nature they often become a threat when swarm of bees attacks a victim by their sting. Mellitin and Phospholipase A2(PLA2) in the bee venom causes pain, inflammation, mild hemorrhage and anaphylactic reaction. Common line of treatment includes hydrocortisone cream and no anti-venom developed yet. Herbal bioactive therapy and complementary medicine remains the option for symptomatic relief of the pain and inflammation associated with insect bites and sting. Chromolaena odorata (L.) (C. odorata) is investigated in the present study to scientifically validate its folkloric use in insect bites. Gel of C. odorata was applied for 7 days in the bare skin of each albino mice after allowing 6 live honey bees to sting on day 1. In egg yolk plate assay, percent inhibition of PLA2 by C. odorata was done. In L929 assay, cell viability was observed after challenge with PLA2 and protection by C. odorata was observed. In the in-vivo mice experiment, significant reduction in erythema and oedema was observed visually and histopathologically. In the In vitro PLA2 inhibition assay, the concentration of 25, 50 and 100 µg of C. odorata extract showed 28.5%, 54.9% and 76.7% inhibition respectively. In the L929 assay, C. odorata neutralised the PLA2 when incubated together and minimize cell death by inhibition of lactate dehydrogenase enzyme. The above results prove its candidature as an alternative medicine for healing from honey bee sting envenomation.

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