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( Toshiyuki Sato ),( Tetsuya Takagawa ),( Yoichi Kakuta ),( Akihiro Nishio ),( Mikio Kawai ),( Koji Kamikozuru ),( Yoko Yokoyama ),( Yuko Kita ),( Takako Miyazaki ),( Masaki Iimuro ),( Nobuyuki Hida ) 대한장연구학회 2017 Intestinal Research Vol.15 No.3
Background/Aims: Recent genome-wide analyses have provided strong evidence concerning adverse events caused by thiopurine drugs such as azathioprine (AZA) and 6-mercaptopurine. The strong associations identified between NUDT15 p.Arg139Cys and thiopurine-induced leukopenia and severe hair loss have been studied and confirmed over the last 2 years. However, other coding variants, including NUDT15 p.Val18_Val19insGlyVal, NUDT15 p.Val18Ile, and FTO p.Ala134Thr, and a noncoding variation in RUNX1 (rs2834826) remain to be examined in detail in this respect. Therefore, we investigated the correlation between these adverse events and the 5 recently identified variants mentioned above among Japanese patients with inflammatory bowel diseases (IBD). Methods: One hundred sixty thiopurine-treated patients with IBD were enrolled. Genotyping was performed using TaqMan SNP Genotyping Assays or Sanger sequencing. Results: None of the 5 variants were associated with gastrointestinal intolerance to AZA. However, NUDT15 p.Arg139Cys was significantly associated with the interval between initiation and discontinuation of AZA among patients with gastrointestinal intolerance. This variant was strongly associated with early (<8 weeks) and late (≥8 weeks) leukopenia and severe hair loss. Moreover, it correlated with the interval between initiation of thiopurine therapy and leukopenia occurrence, and average thiopurine dose. NUDT15 p.Val18_Val19insGlyVal, NUDT15 p.Val18Ile, FTO p.Ala134Thr, and RUNX1 rs2834826 exhibited no significant relationship with the adverse events examined. Conclusions: Of the 5 variants investigated, NUDT15 p.Arg139Cys had the strongest impact on thiopurineinduced leukopenia and severe hair loss; therefore, its genotyping should be prioritized over that of other variants in efforts to predict these adverse events in Japanese patients with IBD. (Intest Res 2017;15:328-337)
High efficiency ester condensation using hydrophobic zeolite membranes
Tomoya Inoue,Yuta Nemoto,Takako Nagase,Yasuhisa Hasegawa,Yoshimichi Kiyozumi,Koichi Sato,Masateru Nishioka,Satoshi Hamakawa,Toshikazu Nishide,Fujio Mizukami 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.3
We successfully developed zeolite membranes with hydrophilic character, by choosing appropriate zeolites in terms of hydrophilicity and high acid tolerance. We evaluated thus developed membranes by their pervaporation (PV) performance, dehydration from acidic organic solvent. The zeolite membranes we developed, based on merlinoite (MER), chabazite (CHA) or phillipsite (PHI), are shown to exhibit stable dehydration performance, respectively. We successfully applied the membranes to the selective removal of water in an ester condensation reaction starting from a stoichiometric mixture of a carboxylic acid and an alcohol. The availability of pervaporation-assisted ester condensation reaction was validated by various kinds of combinations of carboxylic acid and alcohols, which implies the general availability of pervaporation-assisted process intensification by zeolite membranes.
Ajit Kumar Patra,조현희,권용민,권개경,Takako Sato,Chiaki Kato,강성균,김상진 한국해양과학기술원 2016 Ocean science journal Vol.51 No.3
Vestimentiferan tubeworms acquire their symbionts through horizontal transmission from the surrounding environment. In the present study, we constructed a 16S rRNA gene clone library to investigate the phylogenetic relationship between diverse microbes in the sediment and symbiotic bacteria in the trophosome of the tubeworm, Lamellibrachia satsuma, from Kagoshima Bay, Japan. Two symbiotic bacterial phylotypes belonging to the classes γ- and ε-Proteobacteria were found from this tubeworm trophosome. They were very closely related to the symbionts of several other marine invertebrates. The most predominant bacteria in the sediment were ε-Proteobacteria. A broad diversity of bacteria belonged to nonproteobacterial phyla such as Planctomycetes, Acidobacteria, and Chloroflexi was observed. The presence of sulfur oxidizers (i.e., ε-Proteobacteria and γ-Proteobacteria) and sulfur reducers (i.e., δ-Proteobacteria) may play a significant role in the sulfur cycle in these habitats and provide multiple sources of nutrition to the cold-seep communities. Closely related clones of ε-Proteobacteria symbiont in the species level and of γ-Proteobacteria symbiont in the genus level were found in the surrounding sediment. The similarity of symbiont clones of L. satsuma with other symbionts and free-living bacteria suggests the possibility of opportunistic symbiosis in ε-Proteobacteria and the co-evolution of γ-Proteobacteria having occurred after symbiosis with the tubeworms.
Patra, Ajit Kumar,Cho, Hyun Hee,Kwon, Yong Min,Kwon, Kae Kyoung,Sato, Takako,Kato, Chiaki,Kang, Sung Gyun,Kim, Sang-Jin Korean Ocean Research & Development Institute and 2016 OCEAN SCIENCE JOURNAL Vol.51 No.3
Vestimentiferan tubeworms acquire their symbionts through horizontal transmission from the surrounding environment. In the present study, we constructed a 16S rRNA gene clone library to investigate the phylogenetic relationship between diverse microbes in the sediment and symbiotic bacteria in the trophosome of the tubeworm, Lamellibrachia satsuma, from Kagoshima Bay, Japan. Two symbiotic bacterial phylotypes belonging to the classes <TEX>${\gamma}$</TEX>- and <TEX>${\varepsilon}$</TEX>-Proteobacteria were found from this tubeworm trophosome. They were very closely related to the symbionts of several other marine invertebrates. The most predominant bacteria in the sediment were <TEX>${\varepsilon}$</TEX>-Proteobacteria. A broad diversity of bacteria belonged to non-proteobacterial phyla such as Planctomycetes, Acidobacteria, and Chloroflexi was observed. The presence of sulfur oxidizers (i.e., <TEX>${\varepsilon}$</TEX>-Proteobacteria and <TEX>${\gamma}$</TEX>-Proteobacteria) and sulfur reducers (i.e., <TEX>${\delta}$</TEX>-Proteobacteria) may play a significant role in the sulfur cycle in these habitats and provide multiple sources of nutrition to the cold-seep communities. Closely related clones of <TEX>${\varepsilon}$</TEX>-Proteobacteria symbiont in the species level and of <TEX>${\gamma}$</TEX>-Proteobacteria symbiont in the genus level were found in the surrounding sediment. The similarity of symbiont clones of L. satsuma with other symbionts and free-living bacteria suggests the possibility of opportunistic symbiosis in <TEX>${\varepsilon}$</TEX>-Proteobacteria and the co-evolution of <TEX>${\gamma}$</TEX>-Proteobacteria having occurred after symbiosis with the tubeworms.
Himanshu Kumar Bhakta,Pradeep Paudel,Hajime Fujii,Atsuya Sato,박찬흠,Takako Yokozawa,정현아,최재수 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.11
Insulin resistance and protein tyrosine phosphatase1B (PTP1B) overexpression are strongly associatedwith type 2 diabetes mellitus (T2DM), which is characterizedby defects in insulin signaling and glucose intolerance. In a previous study, we demonstrated oligonolinhibits PTP1B and a-glucosidase related to T2DM. In thisstudy, we examined the molecular mechanisms underlyingthe anti-diabetic effects of oligonol in insulin-resistantHepG2 cells. Glucose uptake was assessed using a fluorescentglucose tracer, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose, and the signaling pathwaywas investigated by western blotting. Oligonol significantlyincreased insulin-provoked glucose uptake and decreasedPTP1B expression, followed by modulation of ERKphosphorylation. In addition, oligonol activated insulinreceptor substrate 1 by reducing phosphorylation at serine307 and increasing that at tyrosine 895, and enhanced thephosphorylations of Akt and phosphatidylinositol 3-kinase. Interestingly, it also reduced the expression of two keyenzymes of gluconeogenesis (glucose 6-phosphatase andphosphoenolpyruvate carboxykinase), attenuated oxidativestress by scavenging/inhibiting peroxynitrite, and reactiveoxygen species (ROS) generation, and augmented theexpression of nuclear factor kappa B. These findings suggestoligonol improved the insulin sensitivity of insulinresistantHepG2 cells by attenuating the insulin signalingblockade and modulating glucose uptake and production. Furthermore, oligonol attenuated ROS-related inflammationand prevented oxidative damage in our in vitro modelof type 2 diabetes. These result indicate oligonol haspromising potential as a treatment for T2DM.
Azusa Sasaki,Yasushi Nakamura,Yukiko Kobayashi,Wataru Aoi,Takako Nakamura,Koji Shirota,Noboru Suetome,Michiaki Fukui,Tomoaki Matsuo,Shigehisa Okamoto,Yuri Tashiro,Eun Y. Park,Kenji Sato 한국식품연구원 2018 Journal of Ethnic Foods Vol.5 No.1
Background The fruit of Katsura-uri, traditionally used in the preparation of pickles in Japan, is facing an extinction crisis. In addition to the traditional dishes prepared from Katsura-uri, alternative dishes using the fruit should be devised to increase consumer demands for the protection of the heirloom vegetable. We attempted designing new Katsura-uri contemporary dishes and assessed the application of Katsura-uri juice as a functional drink without raising blood glucose levels. Methods Cooking experiments were conducted with Katsura-uri in its ripening stages, based on the advice from a licensed chef and a registered dietitian in Japan. In the questionnaire-based sensory evaluation, consumer acceptability of Katsura-uri juice was assessed. The blood glucose levels were measured after healthy volunteers consumed the juice. Results We demonstrated six new Katsura-uri dishes. In the questionnaire-based sensory evaluation of Katsura-uri juice, the assessment values for taste and fragrance were high. In human trials, the levels of incremental area under the curve and glucose spike were significantly lower after consumption of Katsura-uri juice, as compared to those after consumption of muskmelon juice. Conclusion Katsura-uri-containing contemporary dishes and juice would help continue the consumption of the vegetable. Based on the results of the questionnaire, we also concluded that the use of Katsura-uri as a functional drink without raising blood glucose levels is superior to its use as contemporary dishes. These findings provide useful strategies to protect Katsura-uri from extinction.