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윤태균(Tae Gyoon Yoon),김현정(Hyun Jeong Kim),염광원(Kwang Won Yum) 대한통증학회 2002 The Korean Journal of Pain Vol.15 No.1
N/A Background: Recently, aminoglycoside antibiotics such as gentamicin, neomycin and amikacin have been known to have antinociceptive effects on several pain models in rats and mice, in addition to their antibacterial activities. However, there has been no report concerning aminoglycoside`s antinociceptive effects on neuropathic pain. The present study was undertaken to assess the antinociceptive action of amikacin and also investigate a possible antinociceptive mechanisms through the use of antagonists in an neuropathic pain models in rats. Methods: Rats were prepared with tight ligation at the left 5^th and 6^th lumbar spinal nerves (according to Kim and Chung`s neuropathic pain model). The antinociceptive effects of amikacin (1, 10, and 100mg/kg i.p.) in rats with neuropathic pain were assessed. In addition, after co-administration of naloxone(1 mg/kg i..p.) with 10 mg/kg of amikacin, the responses to mechanical stimulus were measured over two hours. Results: The antinociceptive effects shown by amikacin on neuropathic pain were significant (P <0.05), but were inhibited by co-administred nalxone in rats with mechanical allodynia. Conclusions: Amikacin showed significant antinociceptive effects in rats with neuropathic pain against mechanical allodynia. The antinociceptive effect on the mechanical stimuli was mediated through the opioid receptor.
Tae Hyun Kim,Yong Seok Choi,Young Hee Choi,Yoon Gyoon Kim 한국독성학회 2013 Toxicological Research Vol.29 No.3
A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of ε-acetamidocaproic acid (AACA), the primary metabolite of zinc acexamate (ZAC), in rat plasma by using normetanephrine as an internal standard. Sample preparation involved protein precipitation using methanol. Separation was achieved on a Gemini-NX C18 column (150 mm × 2.0 mm, i.d., 3 μm particle size) using a mixture of 0.1% formic acid-water : acetonitrile (80 : 20, v/v) as the mobile phase at a flow rate of 200 μl/min. Quantification was performed on a triple quadrupole mass spectrometer employing electrospray ionization and operating in multiple reaction monitoring (MRM) and positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of AACA were linear over the concentration range of 20~5000 ng/ml in rat plasma. The coefficient of variation and relative error at four QC levels were ranged from 1.0% to 5.8% and from .8.4% to 6.6%, respectively. The present method was successfully applied for estimating the pharmacokinetic parameters of AACA following intravenous or oral administration of ZAC to rats.
Pore Structure Control of Ordered Mesoporous Silica Film Using Mixed Surfactants
Ha, Tae-Jung,Im, Hyeon-Gyoon,Yoon, Seok-Jin,Jang, Ho Won,Park, Hyung-Ho Hindawi Publishing Corporation 2011 Journal of nanomaterials Vol.2011 No.-
<P>Materials with nanosized and well-arranged pores have been researched actively in order to be applied to new technology fields. Especially, mesoporous material containing various pore structures is expected to have different pore structure. To form a mixed pore structure, ordered mesoporous silica films were prepared with a mixture of surfactant; Brij-76 and P-123 block copolymer. In mixed surfactant system, mixed pore structure was observed in the region of P-123/(Brij-76 + P-123) with about 50.0 wt.% while a single pore structure was observed in regions which have large difference in ratio between Brij-76 and P-123 through the X-ray diffraction analysis. Regardless of surfactant ratio, porosity was retained almost the same. It is expected that ordered mesoporous silica film with mixed pore structure can be one of the new materials which has distinctive properties.</P>
Kim, Tae Hyun,Choi, Yong Seok,Choi, Young Hee,Kim, Yoon Gyoon Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.3
A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of ${\varepsilon}$-acetamidocaproic acid (AACA), the primary metabolite of zinc acexamate (ZAC), in rat plasma by using normetanephrine as an internal standard. Sample preparation involved protein precipitation using methanol. Separation was achieved on a Gemini-NX $C_{18}$ column ($150mm{\times}2.0mm$, i.d., 3 ${\mu}m$ particle size) using a mixture of 0.1% formic acid-water : acetonitrile (80 : 20, v/v) as the mobile phase at a flow rate of 200 ${\mu}l/min$. Quantification was performed on a triple quadrupole mass spectrometer employing electrospray ionization and operating in multiple reaction monitoring (MRM) and positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of AACA were linear over the concentration range of 20~5000 ng/ml in rat plasma. The coefficient of variation and relative error at four QC levels were ranged from 1.0% to 5.8% and from -8.4% to 6.6%, respectively. The present method was successfully applied for estimating the pharmacokinetic parameters of AACA following intravenous or oral administration of ZAC to rats.
Chung, Eunkyung,Yoon, Tae Gyoon,Kim, Sumin,Kang, Moonkyu,Kim, Hyun Jeong,Son, Youngsook The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.3
This study aimed to investigate the analgesic effect of substance P (SP) in an animal model of neuropathic pain. An experimental model of neuropathic pain, the chronic constriction injury (CCI) model, was established using ICR mice. An intravenous (i.v.) injection of SP (1 nmole/kg) was administered to the mice to examine the analgesic effects of systemic SP on neuropathic pain. Behavioral testing and immunostaining was performed following treatment of the CCI model with SP. SP attenuated mechanical allodynia in a time-dependent manner, beginning at 1 h following administration, peaking at 1 day post-injection, and decaying by 3 days post-injection. The second injection of SP also increased the threshold of mechanical allodynia, with the effects peaking on day 1 and decaying by day 3. A reduction in phospho-ERK and glial fibrillary acidic protein (GFAP) accompanied the attenuation of mechanical allodynia. We have shown for the first time that i.v. administration of substance P attenuated mechanical allodynia in the maintenance phase of neuropathic pain using von Frey's test, and simultaneously reduced levels of phospho-ERK and GFAP, which are representative biochemical markers of neuropathic pain. Importantly, glial cells in the dorsal horn of the spinal cord (L4-L5) of SP-treated CCI mice, expressed the anti-inflammatory cytokine, IL-10, which was not seen in vehicle saline-treated mice. Thus, i.v. administration of substance P may be beneficial for improving the treatment of patients with neuropathic pain, since it decreases the activity of nociceptive factors and increases the expression of anti-nociceptive factors.
쥐의 포르말린시험에서 척수에서의 열충격 단백질들의 발현에 관한 연구
황정태(Jeong Tae Hwang),윤태균(Tae Gyoon Yoon),김현정(Hyun Jeong Kim),염광원(Kwang Won Yum) 대한통증학회 2002 The Korean Journal of Pain Vol.15 No.1
N/A Background: Heat shock proteins (HSPs) are induced in the central nervous system by stressful stimuli are thought to assist in the maintenance of cellular integrity and viability. Recently, several lines of evidence have indicated that HSPs are related to the synaptic in the nervous system, which is related to the nociceptive stimuli induced by formalin injection into rat`s hind paw. Methods: Male Sprague-Dawley rats weighing 250-300g were subcutaneously injected with 100㎕ of fershly made 5% formalin into the right hind paw. At 1 and 24 hour(s) after formalin injection, the right and left sides of the 5^th and 6^th lumbar spinal cord were collected. HSP90, HSP 70, and HSP27 levels were examined using Western blot analysis. Results: Pain related behaviors induced by formalin were typically observed during 1 hr after formalin injection. However, at 24 hr after formalin injeciton, such pain behaviors were rarely observed. Only at 1 hr after formalin injection, HSP70 was significantly more expressed in the ipsilateral and contralateral lumbar spinal cord (P <0.05). The others did not show significant changes in HSPs levels compared to those of the control group. Conclusions: These results suggest that the overexpression of HSP70 in the lumbar spinal cord at 1 hr after formalin injection, which is associated with painful stress, may be related to the molecular mechanism of central sensitization.