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韓聲鎭,元太映 동아대학교 공과대학 부설 한국자원개발연구소 1985 硏究報告 Vol.9 No.2
This paper deals with the magnetostatic field problem via B. E. M A 2-D Z-directional symmetry model which has the current source with the rectangular area is assumed to calculate the vector potential. To examine the accuracy, the B E M solution is compared with the F E M solution showing excellent agreement.
완두 세포질 Fructose-1,6-bisphosphatase의 광의존적 발현
이상원,한태룡 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-
Cytoplasmic fructose-1,6-bisphosphatase from pea leaves was purified and characterized. Monomeric molecular weight of the purified protein was estimated as 37,000 by SDS polyacrylamide gel electrophoresis. The enzyme was active at neutral pH (pH 6.6). The chloroplasmic fructose-l,6-bisphosphatase, isoenzyme of this enzyme, is known to be active at alkaline pH (pH 8.8). The purified enzyme was relatively stable against heat. The activity of the enzyme was not affected by reducing agent such as dithiothreitol and the enzyme was inhibited by AMP and fructose-2,6-bisphosphate as all other gluconeogenic fructose-l,6-bisphosphatase from various species. The enzyme activity of cytoplasmic fructose-1,6-bisphosphatase was gradually increased by light illumination and decreased by dark treatment. Immunoblot analysis suggests that the expression of the enzyme is stimulated by light. Light pulse experiments with blue (420nm), and red (660 nm)/far-red (730 nm) light were performed to analyze the photoreceptors related to light signal transduction and photomorphogenesis. The cytoplasmic enzyme activity was almost insensitive to blue or red/far-red light pulse, suggesting that the cytoplasmic enzyme is not directly regulated by light signal. Thus the activity and/or expression of the cytoplasmic enzyme may be closely related to plant growth and development accompanied by continuous light illumination.
최원종 ( Won Zong Choi ),정지헌 ( Ji Hun Jung ),원호근 ( Ho Keun Won ),강정무 ( Zheng Wu Kang ),한태욱 ( Tae Wook Hahn ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1
Salmonella infections cause the disease in pigs but also some zoonotic Salmonella sero-types can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detec-tion of invA gene using Salmonella specific polymerase chain reaction(PCR), the epidemio-logical characteristics related to Salmonella strains cultured from pig samples in Gang-won areas using serotyping, random amplified polymorphic DNA(RAPD) and pulsed-field gel electrophoresis(PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine(TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioM?rieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23(52.3%) were S Eingedi, 10(22.7%) S Schwarzengrund, 9(20.5%) S Typhimurium, and 2(4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distin-guishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.