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RISS 인기검색어
- 검색키워드
- 저자 : Swirski, Filip K (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Rapid detection and profiling of cancer cells in fine-needle aspirates.
Lee, Hakho,Yoon, Tae-Jong,Figueiredo, Jose-Luiz,Swirski, Filip K,Weissleder, Ralph National Academy of Sciences 2009 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.106 No.30
<P>There is a growing need for fast, highly sensitive and quantitative technologies to detect and profile unaltered cells in biological samples. Technologies in current clinical use are often time consuming, expensive, or require considerable sample sizes. Here, we report a diagnostic magnetic resonance (DMR) sensor that combines a miniaturized NMR probe with targeted magnetic nanoparticles for detection and molecular profiling of cancer cells. The sensor measures the transverse relaxation rate of water molecules in biological samples in which target cells of interest are labeled with magnetic nanoparticles. We achieved remarkable sensitivity improvements over our prior DMR prototypes by synthesizing new nanoparticles with higher transverse relaxivity and by optimizing assay protocols. We detected as few as 2 cancer cells in 1-microL sample volumes of unprocessed fine-needle aspirates of tumors and profiled the expression of several cellular markers in <15 min.</P>
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Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis
Min, Jouha,Nothing, Maria,Coble, Ben,Zheng, Hui,Park, Jongmin,Im, Hyungsoon,Weber, Georg F.,Castro, Cesar M.,Swirski, Filip K.,Weissleder, Ralph,Lee, Hakho American Chemical Society 2018 ACS NANO Vol.12 No.4
<P>Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages (i) the pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis and (ii) a hybrid magneto-electrochemical sensor for IL-3 detection. The developed platform produces test results within 1 h from native blood samples and detects IL-3 at a sensitivity of <10 pg/mL; this performance is >5-times faster and >10-times more sensitive than conventional enzyme-linked immunoadsorbent assays, the current gold standard. Using clinical samples, we show that elevated plasma IL-3 levels are associated with high organ failure rate and thus greater risk of mortality, confirming the potential of IL-3 as a sepsis diagnostic biomarker. With further system development (<I>e</I>.<I>g</I>., full automation, data security measures) and rigorous validation studies, the compact and fast IBS could be a practical clinical tool for timely diagnosis and proactive treatment of sepsis.</P> [FIG OMISSION]</BR>
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