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Duong Thi Thuy Dinh,Suyama Prasansali Mihindukulas,Jinhee Cho,Kalahe Hewage Iresha Nadeeka M,Hyo Jin Kim,Mi-Ok Ko,You-Jin Jeon,Ginnae Ahn,Youngheun Jee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Atopic dermatitis (AD) is one of major skin inflammatory diseases characterized by excessive Th2-mediated immune responses. Recent evidence provides that interlukin-13 (IL-13) plays the role of a key Th2 cytokine that drives the inflammation underlining AD. Due to adverse effects of commercially available synthetic drugs, the need for treatments based on natural products is gaining much attention. Sargassum horneri is an edible brown algae known for beneficial bioactivities including anti-inflammation. We investigated if polyphenol-rich S. horneri extracts (SHE) could suppress AD-like skin lesions in NC/Nga mice and if that involved inhibition of the infiltration of Th2-mediated cytokine IL-13. We observed markedly increased infiltration of IL-13 positive cells in AD-like skin lesions of mice but SHE treatments decreased it. Also, the dermal expression of IL-13 was sufficient to cause inflammatory responses in mice skins resembling human AD. SHE suppressed the dermal infiltration of inflammatory cells where IL-13 plays a crucial role in skin tissues and in the recruitment of inflammatory cells. Furthermore, it was confirmed that SHE reduced T cell, dendritic cell, and macrophage populations in spleens. Moreover, SHE decreased the collagen deposition in skin and ear dermis resulting in reduced fibrosis that occurs in AD due to excessive collagen. Taken together, our results reveal that SHE suppressed the infiltration of inflammatory cells into skin dermis by decreasing the infiltration of IL-13 positive cells. Therefore, SHE could be taken as a useful therapeutic agent to alleviate AD.
The role of IL-17A in particulate matter-exacerbated inflammatory response of allergic asthma mice
Hyo Jin Kim,Kalahe Hewage Iresha Nadeeka M,Areum Kim,Suyama Prasansali Mihindukulas,Duong Thi Thuy Dinh,Jiwon Yang,Anil Poudel,You-Jin Jeon,Youngheun Jee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Particulate matter (PM) aggravated airway and lung inflammatory response in asthma. Asthma could be induced by Th17 related cytokine signaling and IL-17A is a key pro-inflammatory cytokine in the Th17 pathway. In this study, we investigated the mechanism for the role of IL-17A in PM exacerbated pathogenesis of allergic asthma. In brief, 7-8 weeks old BALB/c female mice were randomly divided into the following groups: healthy control, PM only, OVA only, OVA+PM. To establish an asthma mice model, all mice except those in healthy control and PM only groups were sensitized with 10μg of ovalbumin (OVA). Two weeks after the sensitization, mice except those in healthy control and OVA only groups were further exposed to PM (5 mg/m3, 30 min/day) for 7 days. OVA+PM group showed a significant increase in inflammatory cell infiltration and the secretion of mucus and goblet cell hyperplasia. Interestingly, IL-17A expression was significantly increased in OVE+PM, but IL-22 expression was not changed in PM-exposed to lung. Moreover, OVA+PM significantly exacerbated the mRNA expression levels of IL-1β, TNF-α, and IL-6 compared to those in OVA only. The anti-inflammatory cytokine such as IL-10 was suppressed in OVA only and OVA+PM compare to healthy control. Also, OVA only and OVA+PM promoted the expression of epithelial cell-derived pro-allergic cytokines (TSLP, IL-25, IL-33). These results suggested that IL-17 plays an important role in regulating inflammatory response in PM-induced allergic asthma mice. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1A2C1012830).