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Ambient Temperature-Responsive Mechanisms Coordinate Regulation of Flowering Time
Susila, Hendry,Nasim, Zeeshan,Ahn, Ji Hoon MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.10
<P>In plants, environmental conditions such as temperature affect survival, growth, and fitness, particularly during key stages such as seedling growth and reproduction. To survive and thrive in changing conditions, plants have evolved adaptive responses that tightly regulate developmental processes such as hypocotyl elongation and flowering time in response to environmental temperature changes. Increases in temperature, coupled with increasing fluctuations in local climate and weather, severely affect our agricultural systems; therefore, understanding the mechanisms by which plants perceive and respond to temperature is critical for agricultural sustainability. In this review, we summarize recent findings on the molecular mechanisms of ambient temperature perception as well as possible temperature sensing components in plants. Based on recent publications, we highlight several temperature response mechanisms, including the deposition and eviction of histone variants, DNA methylation, alternative splicing, protein degradation, and protein localization. We discuss roles of each proposed temperature-sensing mechanism that affects plant development, with an emphasis on flowering time. Studies of plant ambient temperature responses are advancing rapidly, and this review provides insights for future research aimed at understanding the mechanisms of temperature perception and responses in plants.</P>
Triska Susila Nindya,Trias Mahmudiono,Qonita Rachmah 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.1
Purpose: High intake of cholesterol leads to cardiovascular disruption. Estimating the actual intake of cholesterol can be beneficial for nutrition intervention. This research aimed to develop Semi-Quantitative Food Frequency Questionnaire (SQ-FFQ) to estimate cholesterol intake and analyze its reliability and validity. Methods: SQ-FFQ was developed by sorting high cholesterol food items in Indonesian food database and food items" availability. A total of 30 older adults were randomly chosen from Public Health Center in Jagir District, Surabaya, Indonesia to test its validity. Reliability test was done by measuring the same developed SQ-FFQ in one-month period, while validity test was done by comparing SQ-FFQ results with 6-days food record. Statistical analysis used for reliability test was paired t-test, the Intra-class Correlation Coefficient (ICC), and Cronbach"s α to measure the internal consistency. Meanwhile, validity of developed SQ-FFQ was analyzed using paired t-test and Bland-Altman. Results: Reliability of 2 administered SQ-FFQs showed a good agreement based on paired t-test analysis (p = 0.200), ICC (0.609), and Cronbach"s α (0.757). Strong agreement was found in most of food items, but agreements for egg yolk and fried duck were poor. Significant difference was found between those food items (p = 0.001 vs. p < 0.001, respectively) with mean difference were ?25.3 ㎎ and 46.2 ㎎. Validity of developed SQ-FFQ2 compared to 6-days food diary records also found a strong agreement based on paired t-test and the Bland-Altman analysis. Conclusion: This baseline research provides a reasonably valid and repeatable measure of cholesterol intake estimation that can be widely used in nutrition and public health study, especially in Indonesia. No study has been conducted in Indonesia on the development of tools to estimate the cholesterol intake.
Kusumadewi Yulita,Susila,Henti Hendalastuti Rachmat,Fifi Gus Dwiyanti,Tika D. Atikah,Atok Subiakto,Bayu Pratama,Titiek Setyawati,Wita Wardani,Rizki Ary Fambayun,Nawwall Arrofaha,Irsyad Kama 한국산림과학회 2022 Forest Science And Technology Vol.18 No.4
Dalbergia latifoliais commercial tropical tree species known for its beautiful heartwood,mainly used for furniture and musical instrument. High market demand has put concerns onits sustainability and conservation aspects in Indonesia. Ninety-five specimens ofD. latifoliawere collected from eight populations of Java, Lombok, and Sumbawa Island to study intra-specific variability and diversity using SRAP. One hundred and eighty SRAP loci with 54.03%± 4.35% polymorphism obtained from PCR amplification of 10 primer combinations, with theaverage PIC for these primers of 0.28. Genetic diversity and variability measures were calcu-lated using GenAlEx software indicating a relatively low-mid level of percentage of poly-morphic loci (PPL) (54.03%), effective number of alleles (Ne) (1.255), Shannon informationindex (I) (0.242), and heterozygosity (He) (0.156) on average. The highest value (I¼0.309, He¼0.196) was observed in population P2CJ (Central Java), followed by West Java of P1WJ(I¼0.300, He¼0.191) and West Sumbawa of P7Wsumb (I¼0.257, He¼0.169), while thelowest (I¼0.202, He¼0.129) was found in West Lombok (P4WL). The genetic relationshipswere measured from genetic distance and identity from the two-pairwise calculation, PCoA,and STRUCTURE analysis. The relative homologous population is found between populationsCentral and West Java of P2CJ and P1WJ (Nei uD¼0.031), suggesting these populationshave the same origin and formed one population, while the most distinct (Nei uD¼0.157)was recorded between Sumbawa regency (P6Sumb) and East Java (P3EJ). The results fromPCoA showed that all individuals are placed in three groups almost in accordance with theirrespective islands, i.e. a group comprised inclusively trees from Sumbawa island, a group ofLombok and some Central Java trees, and a group of trees from Java with the exclusion ofsome trees of Central Java that were clustered with trees from Lombok island. This result wasalso supported by STRUCTURE analysis, which showed that Sumbawa, Lombok, and West-East Java populations formed their respective clusters, and Central Java population contains amixture of West-East Java and Lombok populations. This may suggest evidence of complexorigin, thus needing further study to clarify. It is recommended that the populations with thehighest value of genetic diversity (Central and East Java) are to be maintained as the sourceof the gene pool for genetic enrichment programs.
Kei Kawazu,Tarô Adati,Yutaka Yosiyasu,Ketut Sumiartha,Wayan Susila,Putu Sudiarta,Hari Purwanto,Sadahiro Tatsuki 한국응용곤충학회 2009 Journal of Asia-Pacific Entomology Vol.12 No.2
Field tests of three synthetic sex pheromone blends (Japanese blend: Z11–18:Ald (55 μg), Z13–18:Ald (500 μg), Z11–18:OH (120 μg) and Z13–18:OH (180 μg), Indian blend: Z11–16:Ac (50 μg) and Z13–18:Ac (500 μg) and Philippine blend: Z11–16:Ac (500 μg) and Z13–18:Ac (10 μg) based on geographic variations in sex pheromones) of the rice leaffolder moth, Cnaphalocrocis medinalis Guenée (Lepidoptera: Crambidae), were conducted at Yogyakarta (Java), and at Sempidi and Penatih (Bali), Indonesia. Only the Japanese blend attracted significant numbers of male C. medinalis, while neither the Indian nor the Philippine blend showed any attractiveness to the males. In the GC–MS analysis of a crude extract from pheromone glands of female C. medinalis collected at Sanur, Bali. Indonesia, Z11–18:Ald, Z13–18:Ald, Z11–18:OH and Z13–18:OH were detected at a ratio of 10:100:26:37, and the total amount was approximately 0.8 ng/female. Neither Z11–16: Ac nor Z13–18:Ac were detected. These results suggest that C. medinalis that respond to the Japanese blend are widely distributed from Eastern Asia through Southeast Asia. Field tests of three synthetic sex pheromone blends (Japanese blend: Z11–18:Ald (55 μg), Z13–18:Ald (500 μg), Z11–18:OH (120 μg) and Z13–18:OH (180 μg), Indian blend: Z11–16:Ac (50 μg) and Z13–18:Ac (500 μg) and Philippine blend: Z11–16:Ac (500 μg) and Z13–18:Ac (10 μg) based on geographic variations in sex pheromones) of the rice leaffolder moth, Cnaphalocrocis medinalis Guenée (Lepidoptera: Crambidae), were conducted at Yogyakarta (Java), and at Sempidi and Penatih (Bali), Indonesia. Only the Japanese blend attracted significant numbers of male C. medinalis, while neither the Indian nor the Philippine blend showed any attractiveness to the males. In the GC–MS analysis of a crude extract from pheromone glands of female C. medinalis collected at Sanur, Bali. Indonesia, Z11–18:Ald, Z13–18:Ald, Z11–18:OH and Z13–18:OH were detected at a ratio of 10:100:26:37, and the total amount was approximately 0.8 ng/female. Neither Z11–16: Ac nor Z13–18:Ac were detected. These results suggest that C. medinalis that respond to the Japanese blend are widely distributed from Eastern Asia through Southeast Asia.
Qonita Rachmah,Stefania Widya Setyaningtyas,Mahmud Aditya Rifqi,Diah Indriani,Triska Susila Nindya,Hario Megatsari,Trias Mahmudiono,Wantanee Kriengsinyos 대한예방의학회 2019 Journal of Preventive Medicine and Public Health Vol.52 No.4
Objectives: Elderly individuals with diabetes should maintain a normal body mass index (BMI) to help control their blood glucose levels. This study investigated barriers to physical activity (PA), self-efficacy to overcome those barriers, and PA self-efficacy among elderly individuals with diabetes in relation to BMI. Methods: This cross-sectional study included 56 participants. Data were collected by a questionnaire interview and direct measurements for anthropometric data. PA self-efficacy was measured using 8 questions describing different levels of PA, where participants rated the strength of their belief that they could engage in that activity. Self-efficacy to overcome barriers was measured using 10 questions capturing participants’ confidence in their ability to engage in PA despite different possible barriers. Mean scores for these parameters were analyzed using the chi-square test and the independent t-test. Results: In total, 89.3% of participants had a low PA level and 58.9% had more than 3 hours of sedentary activity per day. Furthermore, 55.4% were obese and 14.3% were overweight. The mean scores for PA self-efficacy and self-efficacy to overcome barriers were 59.1±26.4 and 52.5±13.8, respectively. PA level was related to BMI (p<0.001; r=0.116) and sedentary activity (p<0.05; r=0.274). PA self-efficacy and age were not related to BMI. Barriers to PA were associated with PA levels (p<0.05). Conclusions: Physical inactivity was a major problem in elderly individuals with diabetes, and was correlated with higher BMI. Lower levels of PA might be mediated by sedentary activity.