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Jen Fuk Liem,Dwi A. Suryandari,Safarina G. Malik,Muchtaruddin Mansyur,Dewi S. Soemarko,Aria Kekalih,Imam Subekti,Franciscus D. Suyatna,Bertha Pangaribuan 대한예방의학회 2022 예방의학회지 Vol.55 No.3
Objectives: One of the most widely used pesticides today is chlorpyrifos (CPF). Cytochrome P450 (CYP)2B6, the most prominent catalyst in CPF bioactivation, is highly polymorphic. The objective of our study was to evaluate the role of CYP2B6*6, which contains both 516G>T and 785A>G polymorphisms, in CPF toxicity, as represented by the concentration of 3,5,6-trichloro-2-pyridinol (TCPy), among vegetable farmers in Central Java, Indonesia, where CPF has been commonly used. Methods: A cross-sectional study was conducted among 132 vegetable farmers. Individual socio-demographic and occupational characteristics, as determinants of TCPy levels, were obtained using a structured interviewer-administered questionnaire and subsequently used to estimate the cumulative exposure level (CEL). TCPy levels were detected with liquid chromatography-mass spectrometry. CYP2B6*6 gene polymorphisms were analyzed using a TaqMan® SNP Genotyping Assay and Sanger sequencing. Linear regression analysis was performed to analyze the association between TCPy, as a biomarker of CPF exposure, and its determinants. Results: The prevalence of CYP2B6*6 polymorphisms was 31% for *1/*1, 51% for *1/*6, and 18% for *6/*6. TCPy concentrations were higher among participants with CYP2B6*1/*1 than among those with *1/*6 or *6/*6 genotypes. CYP2B6*6 gene polymorphisms, smoking, CEL, body mass index, and spraying time were retained in the final linear regression model as determinants of TCPy. Conclusions: The results suggest that CYP2B6*6 gene polymorphisms may play an important role in influencing susceptibility to CPF exposure. CYP2B6*6 gene polymorphisms together with CEL, smoking habits, body mass index, and spraying time were the determinants of urinary TCPy concentrations, as a biomarker of CPF toxicity.
Wiweko, Budi,Soebijanto, Soegiharto,Boediono, Arief,Mansyur, Muchtaruddin,Siregar, Nuryati C,Suryandari, Dwi Anita,Aulia, Ahmad,Djuwantono, Tono,Affandi, Biran The Korean Society for Reproductive Medicine 2019 Clinical and Experimental Reproductive Medicine Vol.46 No.4
Objective: This study aimed to examine the effect of vitrification on apoptosis and survival in human preantral follicles after thawing. Methods: This experimental study was conducted at an acute tertiary care hospital from March 2012 to April 2013. Ovaries were sliced into 5 × 5 × 1-mm pieces and divided into the following three groups: preantral follicle isolation, ovarian tissue vitrification-warming followed by follicle isolation, and immunohistochemistry of fresh ovarian tissue. For statistical analyses, the Student t-test, chi-square test, Kruskal-Wallis test, and Kaplan-Meier survival analysis were used. Results: A total of 161 preantral follicles (70% secondary) were collected from ovarian cortex tissue of six women between 30 and 37 years of age who underwent oophorectomy due to cervical cancer or breast cancer. There were no significant differences in the follicular morphology of fresh preantral follicles and vitrified follicles after thawing. The mean Fas ligand (FasL) mRNA expression level was 0.43 ± 0.20 (relative to β-actin) in fresh preantral follicles versus 0.51 ± 0.20 in vitrified follicles (p= 0.22). The mean caspase-3 mRNA expression level in fresh preantral follicles was 0.56 ± 0.49 vs. 0.27 ± 0.21 in vitrified follicles (p= 0.233). One vitrified-thawed secondary follicle grew and developed to an antral follicle within 6 days of culture. Conclusion: Vitrification did not affect preantral follicle morphology or mRNA expression of the apoptosis markers FasL and caspase-3. Further studies are required to establish whether vitrification affects the outcomes of in vitro culture and the maturation of preantral follicles.
Lindawati S. Kusdhany,Elza I. Auerkari,Dwi A. Suryandari,Tribudi W. Rahardjo,Eef Hogervoorst,Christopher Talbot 대한예방치과학회 2011 International Journal of Clinical Preventive Denti Vol.7 No.1
Objective: Osteoporosis is a disease of bone metabolism, characterized by reduced density of e.g. the jawbone, with decreasing stability of the denture. Polymorphism in the estrogen receptor β gene (ER-β) has been linked with susceptibility to osteoporosis in ageing persons. The present study aimed to evaluate the association between the ER-β gene polymorphism and osteoporosis in Indonesian postmenopausal women. Methods: The research was a cross-sectional study of 288 subjects that were 47-75 years old. Bone density was determined by an ultrasonic technique and the subjects were divided on this basis into normal, osteopenia and osteoporosis groups. Peripheral blood samples were used for DNA isolation, and the ER-β polymorphism status of the subjects was evaluated using the PCR-RFLP technique with Alu1 restriction enzyme for cutting. Chi-square testing was used to evaluate the relation between the polymorphism status and the observed bone density. Results: The results showed that the status of ER-β polymorphism is significantly associated with osteoporosis in Indonesian postmenopausal women. In particular, a high fraction (68%) of subjects with osteoporosis were of the AA genotype, while osteoporosis was less common (32%) in the Aa genotype and apparently rare in the aa genotype that was in case uncommon. Conclusion: The majority of subjects with osteoporosis were of the AA genotype in terms of ER-β polymorphisms in Indonesian postmenopausal women. As the majority of all subjects were of AA genotype, the results support the view that expression of ER-β would increase the risk to osteoporosis and fracture in ageing women.