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Spontaneous Ovarian Mucinous Adenocarcinama in a Mongolian Gerbil (Meriones unguiculatus)
Sunhwa Hong,Hyun-A Lee,Okjin Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4
An ovarian mucinous adenocarcinoma was found in a 5-month-old female Mongolian gerbil (Meriones unguiculatus). The animal was evaluated because of slowly progressive abdominal enlargement. Postmortem examination revealed clear, pale, straw-colored fluid in the peritoneal cavity. The left ovary was very huge size as 2x5x3 cm and nodular shaped. Histologically, the tumor was composed of various sizes of multilocular cystic glands lined by a single layer of mucin-filled epithelium. Each of these cystic glands was surrounded by a large amount of solid fibrous stroma resembling smooth muscle. Also, undifferentiated tumor cells were found frequently. The ovarian surface epithelium showed partial invasiveness into the ovarian cortex, and a transition was observed between the surface epithelium and the mucinous cyst-forming epithelium. On the basis of histopathological findings, this case was diagnosed as an ovarian mucinous adenocarcinoma. To our knowledge, this report may be the first case of mucinous adenocarcinoma in Mongolian gerbil.
Hong, Sunhwa,Park, Sang-Ho,Chung, Yung-Ho,Kim, Okjin The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.4
Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifically to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma genus-specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.