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Estimating population density of Nicrophorus quadripunctatus (Coleoptera: Silphidae) in Korea
Kim Do‐Sung,Cho Young‐Bok,Han Yong‐Gu 한국곤충학회 2020 Entomological Research Vol.50 No.11
Estimating the population density of organisms is of great concern to ecologists and much effort has been put into making accurate assessment of population levels in nature. We evaluated the trapping web (TW) method for the determination of the density of species of burying beetles. We found eight and six species of burying beetles at Beopjusa and Ssanggok‐ri areas, respectively. Most of the species population comprised Nicrophorus quadripunctatus, with densities of 65 and 195 per ha for Beopjusa and Ssanggok‐ri areas, respectively. Moreover, the flight intercept trap (FIT) method from 2003 to 2007 also revealed the same trend: Beopjusa temple (2928) to Ssanggok‐ri (5835). However, the differences in number of individuals were probably due to the differences in the methods and the length of the trapping period. The populations of N. quadripunctatus exist as metapopulations, making accurate assessment of the population densities extremely difficult. We hypothesized that the TW method could accurately estimate population densities extrapolated from patch population size per area.
( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2
Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.
Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.
Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.
Cho, Kyoung-Suok,Kang, Suk-Gu,Yoo, Do-Sung,Huh, Pil-Woo,Kim, Dal-Soo,Lee, Sang-Bok The Korean Neurosurgical Society 2009 Journal of Korean neurosurgical society Vol.46 No.5
Objective : The present study analyzed the risk factors, prevalence and clinical results following revision surgery for adjacent segment degeneration (ASD) in patients who had undergone lumbar fusion. Methods : Over an 8-year period, we performed posterior lumbar fusion in 81 patients. Patients were followed a minimum of 2 years (mean 5.5 years). During that time, 9 patients required revision surgery due to ASD development. Four patients underwent autogenous posterolateral arthrodesis and extended transpedicle screw fixation, 4 patients underwent decompressive laminectomy and interspinous device implantation, and 1 patient underwent simple decompression. Results : Of the 9 of patients with clinical ASD, 33.3% (3 of 9) of patients did not have radiographic ASD on plain radiographs. Following revision surgery, the clinical results were excellent or good in 8 patients (88.9%). Age > 50 years at primary surgery was a significant risk factor for ASD development, while number of fusion levels, initial diagnosis and type of fusion were not. Conclusion : The incidence of ASD development after lumbar surgery was 11.1% (9 of 81) in this study. Age greater than 50 was the statistically significant risk factor for ASD development. Similar successful clinical outcomes were observed after extended fusion with wide decompression or after interspinous device implantation. Given the latter procedure is less invasive, the findings suggest it may be considered a treatment alternative in selected cases but it needs further study.
Sang-Hyun Oh,Eui-Seong Hwang,Eun-Seok Choi,Gyu-Dong Park,Jin-Gu Kim,Jin-Yong Seong,Jun-Hee Cho,Keun-Do Ban,Keun-Hwan Noh,Nam-Kyeong Kim,Seaung-Suk Lee,Seok-Won Lee,Seung-Jin Yeom,Soon-Yong Kweon,Suk-K 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.IV
We have investigated the ferroelectric properties of integrated Pt/SrBi2Ta2O9 (SBT)/Pt capacitors with capacitor-level dielectric of boron phosphosilicate glass (BPSG). A signicant reduction of remanent polarization (P-P^) was observed in the SBT capacitor as covered with BPSG lm and the polarization decay depended strongly on top electorde size. Detailed analyses showed the degradation resulted from bismuth loss in the surface region of SBT, which is closely related with the impurites diused from BPSG into SBT layer. The degradation of Pt/SBT/Pt ferroelectric capacitor was successfully prevented by inserting SiO2 layer as a impurity blocking layer between the SBT and the BPSG. Thereby device performances of SBT-based ferroelectric memory were also considerably improved.
Experiment for Magnetostriction of Sm-Fe Thin Filmsfor MEMS Actuator
Sang-Joon Lee(이상준),Heung-Shik Lee(이흥식),Chongdu Cho(조종두),Myoung-Gu Kim(김명구) 대한기계학회 2005 대한기계학회 춘추학술대회 Vol.2005 No.5
In this paper, Sm-Fe thin films of 1000A thickness are fabricated by DC magnetron sputtering. Sm-Fe thin films are prepared by DC magnetron sputtering method in the composition of SmxFe1-x (x = 0.4). The thin films are deposited to a thickness of 1000A on Si substrate with a thickness of 50m. The fabricated microstructures are observed by X-ray diffraction (XRD) and the film thickness is measured by using a Ellipsometer. The compositions of sputtered thin films are examined by energy dispersive spectroscopy (EDS). Magnetostrictions are determined from the curvature of the thin films which are measured by the optical cantilever method. The experimental results are discussed with numerical data.
Sang Kyun Cho(趙湘均),Young Jin oh(吳永鎭),Sug Kee Suh(徐錫埼),Hak Sin Kim(金學信),Soo Dong Kim(金洙東),Young Sun Chang(張榮宣),Kyeong Gu Choi(崔京求) 한국육종학회 1995 한국육종학회지 Vol.27 No.2
Improvement of sprout soybeans could be accomplished by maintenance and collection of varieties which have characteristics of small seed, disease resistance and high yielding ability. This research was conducted to investigate morphological and ecological characters for 102 sprout soybean varieties collected in Chonnam and Chonbuk area, and donated by Sprout Soybean Association and Genetic Engineering Research Institute. The sprout soybean collections contained 85% purple flower color, 76% ring-type leaf, 68% brown trichome, 57% elliptical-type seed, 69% yellow seed color and 44% brown hilum color. Days to flowering ranged from 53 to 79 days and growth duration averaged 137 days, with the range of 119~149 days. Stem length ranged from 52㎝ to 165㎝ and 100-seed weight distributed into 4.9~13.3g. The grain yield revealed large range of 0.53~4.18MT/ha. Crude protein and lipid contents was ranged 35.8~48.7% (average 40.9%), and 19.1~’21.2% (average 20.1%), respectively. Correlation coefficients between grain yield and pod numbers was significantly positive, while that between 100-seed weight and pod numbers was significantly nagative. Crude protein contents was significantly associated with growth duration but negatively correlated with 100-seed weight and yield. Crude lipid contents was negatively correlated with growth duration but positively associated with 100-seed weight.