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      • KCI등재

        Efficacy of hyaluronic acid film on perianal wound healing in a rat model

        Jung Rae Cho,Myoung-Han Lee,Heung-Kwon Oh,Hyojin Kim,Dong-Keon Kweon,So Min Kang,Baek Kyu Kim,Chan Yeong Heo,Duck-Woo Kim,Sung-Bum Kang 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.101 No.4

        Purpose: Postoperative pain and delayed wound healing are the main complications following anal surgery associated with poor quality of life. Hyaluronic acid (HA) supports tissue regeneration and rapid wound healing by promoting cell proliferation and migration. We investigated the effects of HA on perianal wound healing in a rat model. Methods: Forty-eight 8-week-old Sprague-Dawley rats with perianal wounds created by biopsy punch were divided into 3 groups: simple dressing with gauze (control), dressing with topical HA film, and dressing with topical HA gel. HA agents were not reapplied postoperatively. Wound healing was evaluated by measuring the healed area, and histological analyses were randomly performed using hematoxylin and eosin and Masson trichrome staining. Results: Fewer mean days were required for complete wound healing in the HA film and HA gel groups than in the control group (11.6 vs. 11.9 vs. 13.8 days, respectively; P = 0.010). The healed area in the HA film group on day 11 was larger than that in the HA gel and control groups (80.2% vs. 61.9% vs. 53.2%, respectively; P < 0.001). Histologically, the HA film group showed accelerated reepithelialization, a rapid transition to lymphocyte-predominant inflammation, and increased fibroblastic proliferation and collagen deposition compared to the other groups. There was no treatment-related toxicity in the HA application groups. Conclusion: Topical application of HA film to perianal wounds improves the wound healing rate in a rat model. This finding suggests a potential benefit of HA film application in promoting wound healing after anal surgery in humans.

      • SCIESCOPUSKCI등재

        The Effect of Environmentally Friendly Wallpaper and Flooring Material on Indoor Air Quality and Atopic Dermatitis: A Pilot Study

        ( Jung Im Na ),( Sang Young Byun ),( Mi Young Jeong ),( Kyoung Chan Park ),( Chang Hun Huh ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.6

        Background: Formaldehyde (FA) and other volatile organic compounds (VOCs) are considered among the main causes of atopic aggravation. Their main sources include wallpapers, paints, adhesives, and flooring materials. Objective: To assess the effects of environmentally friendly wallpaper and flooring material on indoor air quality and atopic dermatitis severity. Methods: Thirty patients with atopic dermatitis were enrolled in this study. To improve air quality, the wallpaper and flooring in the homes of the subjects were replaced with plant- or silica-based materials. The indoor air concentration of FA and the total VOCs (TVOCs) were measured before remodeling and 2, 6, and 10 weeks thereafter. Pruritus and the severity of atopic eczema were evaluated by using a questionnaire and the eczema area and severity index (EASI) score before and at 4, 8, and 12 weeks after remodeling. The subjects were instructed to continue their therapy for atopic dermatitis. Results: The houses of 24 subjects were remodeled; all subjects completed the study. The concentration of FA in ambient air significantly decreased within 2 weeks after remodeling. The TVOC level showed a decrease at week 2 but increased again at weeks 6 and 10. The reduction of pruritus and EASI score was statistically significant in patients whose baseline EASI score was >3. Conclusion: Replacing the wallpaper and flooring of houses with environmentally friendly material reduced FA in ambient air and improved pruritus and the severity of atopic eczema. The improvement of pruritus and eczema was statistically significant in patients whose baseline EASI score was >3. (Ann Dermatol 26(6) 688∼692, 2014)

      • KCI등재

        Efficacy of antagonistic bacteria and commercial microbial pesticide for biological control of mushroom disease

        Chan-Jung Lee,Hye-Su Han,Chang-Sung Jhune,Jong-Chun Cheong,Jin-A Oh,Na-Na Um,Hang-Yeon Weon 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

        This study was carried out investigate to availability of commercial microbial pesticide and antibacterial activity of isolates isolated from different mushroom media. Ten commercial microbial pesticide and EM liquid were collected from different company. EM of these one has been used for control of mushroom disease and growth promotion at the mushroom farms. The density of bacteria and yeast in EM cultural liquid was higher than those of the crude liquid. The pH values of EM showed the low acid levels(pH 3.5~3.9) by organic acid secreted from microorganism. The kinds of organic acid was acetic acid, lactic acid and succinic acid. The dominant bacteria isolated from EM liquid was Lactobactillus sp.(21 strains), Acetobacter sp.(9 strains), PaeniBacillus sp.(9 strains) and others(12 strains). The organic acid bacteria isolated from fermentation foods( was 92 strains and the dominant genus was Weissella sp.(41 strains), Leuconostoc sp.(21 strains), Enterococcus sp.(9 strains), Lactococcus sp.(9 strains) and others(12 strains). And we isolated 2,500 bacteria from oyster mushroom and button mushroom cultural media for selection of antagonistic bacteria. Thirty five strains of these isolates showed very strong antagonistic activity. These strains were identified Bacillus amyloliquefaciens, Bacillus subtilis, Brevibacterium halotolerans, Pseudomonas libanensis, Stenotrophomonas maltophilia and Alcaligenes faecalis by 16S rDNA analysis.

      • SCIESCOPUSKCI등재

        Effects of Repeated Citalopram Treatments on Chronic Mild Stress- Induced Growth Associated Protein-43 mRNA Expression in Rat Hippocampus

        Sang-Ha Park,Song-hyen Choi,Ji min Lee,Seung woo Kang,You-Chan Shin,Hyun-Ju Kim,Hyun Jung Kim,Seung Keon Shin,Min-Soo Lee,Kyung-Ho Shin 대한생리학회-대한약리학회 2008 The Korean Journal of Physiology & Pharmacology Vol.12 No.3

        Although growth associated protein-43 (GAP-43) is known to play a significant role in the regulation of axonal growth and the formation of new neuronal connections in the hippocampus, there is only a few studies on the effects of acute stress on GAP-43 mRNA expression in the hippocampus. Moreover, the effects of repeated citalopram treatment on chronic mild stress (CMS)-induced changes in GAP-43 mRNA expression in the hippocampus have not been explored before. To explore this question, male rats were exposed to acute immobilization stress or CMS. Also, citalopram was given prior to stress everyday during CMS procedures. Acute immobilization stress significantly increased GAP-43 mRNA expression in all subfields of the hippocampus, while CMS significantly decreased GAP-43 mRNA expression in the dentate granule cell layer (GCL). Repeated citalopram treatment decreased GAP-43 mRNA expression in the GCL compared with unstressed controls, but this decrease was not further potentiated by CMS exposure. Similar decreases in GAP-43 mRNA expression were observed in CA1, CA3 and CA4 areas of the hippocampus only after repeated citalopram treatment in CMS-exposed rats. This result indicates that GAP-43 mRNA expression in the hippocampus may differently respond to acute and chronic stress, and that repeated citalopram treatment does not change CMS-induced decreases in GAP-43 mRNA expression in the GCL.

      • Transcriptional changes of three Vitis PIN genes in grapevines during inflorescence development upon gibberellin application at pre-bloom

        Chan Jin Jung,Youn Young Hur,Sung-Min Jung,Hee Jae Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

        The tight regulators of fruit set initiation, gibberellin (GA) and auxin, have been applied for decades to induce parthenocarpy, fruit set without fertilization. The integration of GA and auxin signaling mediated by either GA or auxin application during parthenocarpy has been actively reported in tomato, and recently we reported that GA application at pre-bloom also activating auxin signaling and down-regulated negative regulators of fruit set initiation in grapevines. However, the activation of auxin signaling upon GA application without up-regulation of auxin biosynthesis is still unclear. In this study, expression patterns of three auxin efflux transporter genes, VvPIN1a, VvPIN2 and VvPIN4, were monitored during inflorescence development in ‘Tamnara’ grapevines with or without GA application. Without GA application, transcription levels of VvPIN1a and VvPIN4 gradually increased from 14 days before full bloom (DBF) to 2 and 5 days after full bloom (DAF), respectively, except down-regulation of VvPIN1a during 5 DBF to full bloom. However, VvPIN2 expression declined steadily after peaking at 10 DBF. With GA application, VvPIN1a did not show significantly different expression patterns when compared to no GA application, with the exception of 4-fold up-regulation at full bloom, but transcription of VvPIN4 was reduced between 5 and 2 DBF. In addition, VvPIN2 was down-regulated between 12 and 10 DBF by more than 50% compared to levels in the absence of GA application. These reductions of both VvPIN2 and VvPIN4 with GA application prior to pollination suggest that GA application might regulate auxin distribution, instead of auxin biosynthesis, to activating auxin signaling during parthenocarpic fruit initiation.

      • KCI등재

        Changes and distributional pattern of microorganism in compost and fruiting body for button mushroom cultivation

        Chan-Jung Lee,Na-Na Um,Jong-Chun Cheong,Chang-Sung Jhune,Jin-A Oh,Hye-Su Han,Byeong-Yeol, Yeon 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

        Agaricus bisporus grows on a substrate known as compost, which is a product of aerobic fermentation by various microorganisms. These organisms convert and degrade the straw and form lignin humus complex which is utilized later on by the population of organisms. Theses microflora play a key role in the process of composting and can be regarded as the active agents in the preparation of nutrient medium as many of them may ultimately contribute themselves to the nutrition of A. bisporus. The diversity of microflora according to growing farmhouse and fruiting body of Agaricus bisporus were investigated. The aerobic bacteria and Bacillus as longer of turning stage of compost pile were increased. And, thermophilic actinomycetes and fluorescent Pseudomonas sp. showed high density after the pasteurization stage. But Tricoderma sp. was decreased toward the end of turning stage of compost pile. Ten mushroom farms was selected to research of microflora of fruiting body of button mushroom. The microflora showed significant difference according to mushroom farms. The bacteria density was 0.4~41.6×105 cfu/ml and the fungus was 1.3~3.9×103 cfu/ml. But The microorganism density was not significant change for the storage periods. These isolates were classified into Chryseobacterium indologenes(6 strains), Pseudomonas agarici(5 strains), Sphingobacterium multivorum(2 strains), Flavobacterium anhuiense(2 strains), Microbacterium sp.(10 strains), Pseudomonas sp.(13 strains) on the basis of 16 rDNA analysis. The most dominants of these species were Chryseobacterium indologenes and Pseudomonas agarici.

      • Comparison of the Immediate Effects of Functional Massage and Stretching Methods on the SRT, Knee Angles, and Leg Lengths of Adults with Insufficient Exercises

        Chan-woo Nam,Youn-Bum Sung,Jung-Ho Lee 보안공학연구지원센터 2016 International Journal of Software Engineering and Vol.10 No.11

        In the modern society, as the quality of life has been improving and social interest in health and well-being has been increasing, the importance of exercise has been emphasized so that many people actively participate in exercise and conduct many studies on physical activities and exercise. Therefore, in the present study, the lower extremities that are closely related to walking out of the body and can be easily damaged during daily living or sports activities were selected to conduct experiments to apply functional massage and stretching to the lower extremities of adults with insufficient lower extremity exercise in order to compare immediate effects with a view to making activities good before starting daily living or outdoor activities, or sports activities. The subjects were divided into a functional massage group(n: 30) and a stretching group(n: 30) and the participants participated in the experiment through voluntary applications after firsthand hearing explanations about the experiment from the therapist. In order to examine differences in the effects of the intervention between the Functional massage and stretching groups, the independent samples t-test and paired t-tests were performed. An experiment was conducted with functional massage and the results showed statistically significant differences in all comparison items. This may mean that the improvement of overall lower extremity functions and the intervention method stretching could be also regarded to be a very effective method for improvement of lower extremity functions.

      • KCI등재

        Identification and Characterization of Burkholderia gladioli pv. agaricicola Causing Bacterial Soft Rot of Cultivated Mushroom, Pleurotus ostreatus, in Korea

        Chan-Jung Lee,Hyung-Sik Yun,Chang-Sung Jhune,Jong-Chun Cheong 한국버섯학회 2009 한국버섯학회지 Vol.7 No.2

        Mushroom is cultivated in many areas of the Korea as one of the major economical crops. The production areas have steadily increased approximately 3,674 ha in 2002 to 4,118 ha in 2005. Several bacteria have been known as the causal agents of certain diseases of cultivated button mushroom, Agaricus bisporus, and oyster mushroom, Pleurotus ostreatus. It is well known as bacterial diseases of the cultivated mushroom such asbrown blotch, mummy disease, bacterial pit, bacterial rot and weeping disease, ginger blotch, and drippy gill. Unknown soft rot bacterium was isolated from sunken browning symptom of cultivated oyster mushrooms grown in Korea. The symptoms are appear as a sunken browning lesions on the caps of affected mushrooms. The bacterium causes a rapid soft rot of cultivated mushrooms in comparison with brown blotch bacteria at temperatures above 25℃. From these lesions we isolated one bacterial strain (designated OM1). Inoculation of bacterial isolates into mushroom caps yielded characteristic sunken brown, watersoaked and severe soft rot symptoms, but which were indistinguishable in early stage from those of the bacterial brown blotch well known to mushroom growers. Results of Gram stain and biochemical tests identified this isolate as Burkholderia gladioli pv. agaricicola. This was confirmed by pathogenicity, physiological and biochemical characteristics, and results of an analysis of the 16S rRNA gene sequences and the fatty acids profile. This is the first report of the isolation of B. gladioli pv. agaricicola from cultivated oyster mushroom in Korea.

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