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Production and Characterization of Monoclonal Antibodies against Human Ceruloplasmin
Eum, Won-Sik,Choi, Hee-Soon,Kim, Dae-Won,Jang, Sang-Ho,Choi, Soo-Hyun,Kim, So-Young,Park, Jin-Seu,Kang, Jung-Hoon,Cho, Sung-Woo,Kwon, Oh-Shin,Hwang, In-Koo,Yoo, Ki-Yeon,Kang, Tae-Cheon,Won, Moo-Ho,Cho Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.1
Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57%. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.
Electron Microscopy Investigation at the Initial Growth Stage of Carbon Nanotubes
Sung-Jin Eum,양철웅,박종윤,Hee-Jin Jung,Hee-Kwang Kang,Ji-Beom Yoo 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.III
The growth mechanism and the geometry of carbon nanotubes (CNTs) are affected by several parameters such as formation process and catalytic materials. In order to understand the growth mechanism of CNT, it is essential to observe the CNTs at the initial growth stage. We synthesized CNTs directly on the Ni-deposited TEM Cu mesh grid using thermal CVD so as to preserve and investigate the original structure of CNTs from the initial to final growth state without any artifacts that might be introduced during TEM specimen preparation. The results obtained from the electron microscopy study show that the following growth modes of CNTs occur simultaneously at the initial stage: tip-growth, base-growth and intermediate-growth mode. The intermediate growth structure can be achieved by a mixture of base-growth mode and tip-growth mode, which occur sequentially. This is thought to be a probable mechanism for nanotube junctions. The growth mechanism and the geometry of carbon nanotubes (CNTs) are affected by several parameters such as formation process and catalytic materials. In order to understand the growth mechanism of CNT, it is essential to observe the CNTs at the initial growth stage. We synthesized CNTs directly on the Ni-deposited TEM Cu mesh grid using thermal CVD so as to preserve and investigate the original structure of CNTs from the initial to final growth state without any artifacts that might be introduced during TEM specimen preparation. The results obtained from the electron microscopy study show that the following growth modes of CNTs occur simultaneously at the initial stage: tip-growth, base-growth and intermediate-growth mode. The intermediate growth structure can be achieved by a mixture of base-growth mode and tip-growth mode, which occur sequentially. This is thought to be a probable mechanism for nanotube junctions.
Eum, Won Sik,Jang, Sang Ho,Kim, Dae Won,Choi, Hee Soon,Choi, Soo Hyun,Kim, So Young,An, Jae Jin,Lee, Sun Hwa,Han, Kyuhyung,Kang, Jung Hoon,Kang, Tae-Cheon,Won, Moo Ho,Cho, Yong Joon,Choi, Jin Hi,Kim, Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.2
<P>The human immunodeficiency virus type 1 (HIV-1) Tat protein transduction domain (PTD) is responsible for highly efficient protein transduction across plasma membranes. In a previous study, we showed that Tat-Cu,Zn-superoxide dismutase (Tat-SOD) can be directly transduced into mammalian cells across the lipid membrane barrier. In this study, we fused the human SOD gene with a Tat PTD transduction vector at its N- and/or C-terminus. The fusion proteins (Tat-SOD, SOD-Tat, Tat-SOD-Tat) were purified from Escherichia coli and their ability to enter cells in vitro and in vivo compared by Western blotting and immunohistochemistry. The transduction efficiencies and biological activities of the SOD fusion protein with the Tat PTD at either terminus were equivalent and lower than the fusion protein with the Tat PTD at both termini. The availability of a more efficient SOD fusion protein provides a powerful vehicle for therapy in human diseases related to this anti-oxidant enzyme and to reactive oxygen species.</P>
Eum Seok-Yong,Baek Seung-Hun,Hong Min-Sun,Lee Jong-Seok,Cho Eun-Jin,Lee Ji-Im,Cho Sang-Nae,Sun-Kyoung Lee 대한결핵및호흡기학회 2022 Tuberculosis and Respiratory Diseases Vol.85 No.3
Background: The current conventional drug susceptibility test (DST) for <i>Mycobacterium tuberculosis</i> (Mtb) takes several weeks of incubation to obtain results. As a rapid method, molecular DST requires only a few days to get the results but does not fully cover the phenotypic resistance. A new rapid method based on the ability of viable Mtb bacilli to hydrolyze fluorescein diacetate to free fluorescein with detection of fluorescent mycobacteria by flow cytometric analysis, was recently developed.Methods: To evaluate this cytometric method, we tested 39 clinical isolates which were susceptible or resistant to isoniazid (INH) or rifampin (RIF), or ethambutol (EMB) by phenotypic or molecular DST methods and compared the results.Results: The susceptibility was determined by measuring the viability rate of Mtb and all the isolates which were tested with INH, RIF, and EMB showed susceptibility results concordant with those by the phenotypic solid and liquid media methods. The isolates having no mutations in the molecular DST but resistance in the conventional phenotypic DST were also resistant in this cytometric method. These results suggest that the flow cytometric DST method is faster than conventional agar phenotypic DST and may complement the results of molecular DST.Conclusion: In conclusion, the cytometric method could provide quick and more accurate information that would help clinicians to choose more effective drugs.
Associations between Living Alone and Smoking and Alcohol Consumption in Korean Adults
Sang-Woo Lee,Byoungduck Han,Sung Jung Cho,Seung Jin Jung,허연,Junghun Kim,Do Hyun Eum,Taeryoon Kim,Soon-Hong Min,Woohyun Lee,Jinhee Cho,Min Hee Kwon,Ga Eun Nam 대한가정의학회 2020 Korean Journal of Family Medicine Vol.41 No.5
Background: Recently, single-person households have increased in Korea and this trend may have potential public health implications and affect various health behaviors. This study investigated the associations between living alone and health behaviors such as smoking and alcohol consumption among Korean adults. Methods: We used data from the 6th Korea National Health and Nutrition Examination Survey between 2013 and 2015 and a total of 17,088 adults were included. We performed a multivariable logistic regression analysis and calculated odds ratios (ORs) with 95% confidence intervals (CIs). Results: Single-person households accounted for about 10% of the total participants. The proportions of currently smoking and heavy alcohol consumption were higher among individuals living alone than those living together among younger women and middle-aged and elderly men and women. In the multivariable logistic regression analysis, living alone was associated with increased odds of currently smoking compared to living together among middle-aged men and women (OR, 7.37; 95% CI, 2.33–23.32 in men and OR, 2.36; 95% CI, 1.04–5.36 in women) after adjusting for confounding variables. Conclusion: From this nationwide, population-based study, we found that living alone is associated with increased odds of currently smoking, especially in middle-aged people. Public health concerns may be warranted for middleaged single-person households to reduce health risks related to smoking.