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Sung Youn Heo,Dong Hoon Kwak,Yu Na Seo,Won Seok Ju,Hyun Gyu Lim,Seo Yi Lee,Ji-Su Kim,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07
Mesenchymal stem cells can differentiate into multiple lineages depending on their exposure to differing biochemical and biomechanical inductive factors. The Ganglioside is included in biomarker, that are the major component of cytoplasmic cell membranes complex glycosphingolipids, and play a role in the control of biological processes. However, role of ganglioside in osteogenesis of mini-pig adipose derived mesenchymal stem cells (AD-MSCs) unclear . We have knocked down was ganglioside synthase UDP-glucose ceramide glucosyltransferase (UGCG)using a siRNA differentiation into osteoblast was markedly decreased in UGCG-knocked down AD-MSCs. The results showed significant inhibits alkaline phosphatase, osteocalcin, osteopontin, and Runt-related transcription factor 2 in UGCG knock down AD-MSCs compared to with the control AD-MSCs. Thus we guess, maybe ganglioside is essential factor to stimulate osteogenesis in AD-MSCs.
Sung Youn Heo,You Na Seo,Won Seok Ju,Hyun Gyu Lim,Sang-Young Seo,Jin Hyoung Cho,Park Soon Ju,Young-Kug Choo 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Mesenchymal stem cells are undifferentiated stem cells that can differentiate into various cells and tissues. In this study, we confirmed the difference in expression and differentiation rate of gangliosides during the differentiation of mini-pig adipose-derived mesenchymal stem cells into neuronal cells using secretion medium from PMA-induced human macrophage-like cell line U937 (Macro SM). We showed that nm23, one of several proteins secreted in the culture medium of activated human macrophages, decreased the neuronal differentiation rate of mesenchymal stem cells derived from mini-pig adipose, and inhibited the function and the synthesis enzyme of GD3. In conclusion, one of the factors that decreased the differentiation rate of mini-pig adipose-derived mesenchymal stem cells into neuronal cells by Macro SM is the nm23 protein.
Sung Youn Heo,You Na Seo,Won Seok Ju,Hyun Gyu Lim,Sang-Young Seo,Jin Hyoung Cho,Park Soon Ju,Young-Kug Choo 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Macrophages, the cells of the innate immune system, play a key role in all three types of xenograft rejection. However, the mechanism underlying their direct effects is incompletely understood. To study the direct effects of macrophages, established human model system combined the U937 monocyte cell line and mini-pig adipose derived mesenchymal stem cells (mp AD-MSCs). Treatment of mp AD-MSCs with macrophage secretion medium (Macro SM) markedly reduced the cell viability, increased lactate dehydrogenase release, and induced apoptotic cell death. The proteomic analysis identified 17 proteins in Macro SM. Based on the gene ontology analysis tool, matrix metalloproteinase (MMP)-9 was selected as the protein affecting MSC viability. To confirm the effect of MMP-9, cells were treated with recombinant human MMP-9 that resulted in decreased cell viability and increased lactate dehydrogenase release. These results suggest that MMP-9 may decrease the cell viability by damaging the cell membrane in the co-culture model.
SWAT ArcView GIS Extension Patch를 이용한 소유역 분할에 따른 수문 및 유사 거동에 미치는 영향 평가
허성구 ( Sung Gu Heo ),김남원 ( Nam Won Kim ),박윤식 ( Youn Shik Park ),김종건 ( Jong Gun Kim ),김성준 ( Seong Joon Kim ),안재훈 ( Jae Hun Ahn ),김기성 ( Ki Sung Kim ),임경재 ( Kyoung Jae Lim ) 한국물환경학회 2008 한국물환경학회지 Vol.24 No.2
Because of increased nonpoint source runoff potential at highland agricultural fields of Kangwon province, effective agricultural management practices are required to reduce the inflow of sediment and other nonpoint source pollutants into the water bodies. The watershed-scale model, Soil and Water Assessment Tool (SWAT), model has been used worldwide for developing effective watershed management. However, the SWAT model simulated sediment values are significantly affected by the number of subwatershed delineated. This result indicates that the SWAT estimated watershed characteristics from the watershed delineation process affects the soil erosion and sediment behaviors. However, most SWAT users do not spend time and efforts to analyze variations in sediment estimation due to watershed delineation with various threshold value although topography falsification affecting soil erosion process can be caused with watershed delineation processes. The SWAT model estimates the field slope length of Hydrologic Response Unit (HRU) based on average slope of subwatershed within the watershed. Thus the SWAT ArcView GIS Patch, developed by using the regression relationship between average watershed slope and field slope length, was utilized in this study to compare the simulated sediment from various watershed delineation scenarios. Four watershed delineation scenarios were made with various threshold values (700 ha, 300 ha, 100 ha, and 75 ha) and the SWAT estimated flow and sediment values were compared with and without applying the SWAT ArcView GIS Patch. With the SWAT ArcView GIS Patch applied, the simulated flow values are almost same irrespective of the number of subwatershed delineated while the simulated flow values changes to some extent without the SWAT ArcView GIS Patch applied. However when the SWAT ArcView GIS Patch applied, the simulated sediment values vary 9.7% to 29.8% with four watershed delineation scenarios, while the simulated sediment values vary 0.5% to 126.6% without SWAT ArcView GIS applied. As shown, the SWAT estimated flow and sediment values are not affected by the number of watershed delineation significant compared with the estimated flow and sediment value without applying the SWAT ArcView GIS Patch.
Heo, Yu-Jung,Oh, Hye-Jwa,Jung, Young Ok,Cho, Mi-La,Lee, Seon-Yeong,Yu, Jun-Geol,Park, Mi-Kyung,Kim, Hae-Rim,Lee, Sang-Heon,Park, Sung-Hwan,Kim, Ho-Youn BioMed Central 2011 ARTHRITIS RESEARCH AND THERAPY Vol.13 No.4
<P><B>Introduction</B></P><P>The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of arthritis. We conducted this study to determine the effect of interleukin (IL)-17 on the expression and production of RAGE in fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). The role of nuclear factor-κB (NF-κB) activator 1 (Act1) in IL-17-induced RAGE expression in RA-FLS was also evaluated.</P><P><B>Methods</B></P><P>RAGE expression in synovial tissues was assessed by immunohistochemical staining. RAGE mRNA production was determined by real-time polymerase chain reaction. Act-1 short hairpin RNA (shRNA) was produced and treated to evaluate the role of Act-1 on RAGE production.</P><P><B>Results</B></P><P>RAGE, IL-17, and Act-1 expression increased in RA synovium compared to osteoarthritis synovium. RAGE expression and production increased by IL-17 and IL-1β (*<I>P <</I>0.05 vs. untreated cells) treatment but not by tumor necrosis factor (TNF)-α in RA-FLS. The combined stimuli of both IL-17 and IL-1β significantly increased RAGE production compared to a single stimulus with IL-17 or IL-1β alone (<I>P <</I>0.05 vs. 10 ng/ml IL-17). Act-1 shRNA added to the RA-FLS culture supernatant completely suppressed the enhanced production of RAGE induced by IL-17.</P><P><B>Conclusions</B></P><P>RAGE was overexpressed in RA synovial tissues, and RAGE production was stimulated by IL-17 and IL-1β. Act-1 contributed to the stimulatory effect of IL-17 on RAGE production, suggesting a possible inhibitory target for RA treatment.</P>
Sang Hoon Kim,Young Wha Song,Hong Rae Kim,Seo Yoon Heo,Yong Youn Kim 국제물리치료학회 2017 Journal of International Academy of Physical Ther Vol.8 No.1
The purpose of this study was to investigate the effects of hypobarichypoxic training program on competitive performance. This was done by observing their conditioning and measuring their blood constituents before and after a multi-staged intermittent training program, over 2 weeks. Three national handicapped cyclists were placed in a multi-leveled hypobaric-hypoxic (flat–4000 meter (m) high elevation) environment with consistent temperature and humidity (23 ± 2℃, 50 ± 5%) for 2 weeks. After the training, the blood constituents and average heart rate (HR) were measured and the following results were obtained. In all three athletes, there were no unique changes in red blood cell count, hemoglobin, and hematocrit, while there was a rise in the reticulocyte count. Observations of the difference in average HR during exercise at varying altitudes showed that athlete A had an average increase in the HR for the first 5 days at 2000 m. For athlete B, the comparison of the first and last training sessions at an altitude of 2000 m showed an HR increase of approximately 17%. For athlete C, there was a steady increase in the HR until day 7 of the training. As such, hypobarichypoxic training suggested that improvement of aerobic exercise performance in these athletes and it is recommended that there be a development for future training programs at high altitude, geared towards handicapped athletes of various disciplines.