항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열

이대운,윤성준,김우구,신철교,임성환,이백락,문경호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

한국에서 임산균주로부터 분리한 테트라사이클린(Tc) 내성 플라스미드 pKH6의 전체 염기서열을 결정하여 가장 대표적인 Tc 내성 플라스미드인 pT181과 비교하였다. pKH6의 전체 길이는 4439bp로 pT181과 동일하게 나타났으며 염기서열은 7개의 염기에서 차이를 보였다. 전부 다 염기쌍이 치환된 형태로 나타났으며 그 중 3개는 coding 부위에서 일어났다. coding 부위에서 일어난 경우에도 1개는 동일한 아미노산을 coding 하였기 때문에 pT181과 차이가 없었으며 RepC와 Pre 단백질에 있어 각각 1개의 아미노산에서 차이를 나타내었다. The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc^r) plasmid isolated from multi-drug resistant Staphlococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc^r plasmid pT181. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only tow base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.

稻熱病 抵抗性에 關한 硏究

金明運,盧愼圭,白壽鳳,李成泰,任文淳,朴澤奎,李麗夏 건국대학교 1974 學術誌 Vol.17 No.1

This study was made to investigate the degree of rice blast disease developing in various plants and also the relationship between the disease developing and the amount of content of nutrients in rice plants which were differently dressed. Possibility for culture of rice-blast race was studied and the structure of infected leaves were observed by electron microscope. 1) The varieties sampled, Pung-kwang, Jin-heung, Pal-tal, Pung-ok and Tong-il, were seeded and cultivated in pots. When fifth or sixth leaf emerged, the races of blast T,C,N were innoculated in all varieties. In dressing test Pung-ok, which leas susceptible to blast, was dressed with nitrogen fertilizer in ordinary and twofold and in none-dressing or dressing of silicon fertilizer. When the fifth or sixth leaf was appeared, races T, C, N was innoculated in each varieties of pot. In all experiment the degree of infection was checked out according to the order of leaf, and at the same time, the relationship between the degree of disease developing and the contents of nutrients by determining the total nitrogen, total sugar, phosphoric acid, kalium, silicon dioxide soluble nitrogen and soluble sugar was studied. The occurance of symptom was relatively frequent in upper primary or secondary leaf in all varieties regardless of the kind of fertilizers or their dressing amount. Comparing the occurrencies of disease development in examined varieties, Jin heung was lowest and Pung-ok was highest, while Tong-il was completely free from the infection of the disease. The race T-type attacked most seriously the varieties of Jin-heung, Pal-Tal and Pung-kwang except Pung-ok, C-type was moderate and N-type was very weak in Pung-ok. In the amount of dressing, the occurance of disease was very rare in the rice plant of N-ordinary-silicon fertilizer dressed and frequent in that of nitrogen fertilizer-twofold-and-silicon fertilizer none dressed. The disease developing differed with the races, indicating T-type was most poweful, C-type moderate while N-type was weak. In the interaction between the contents of nutrients in riceplant and the degree of disease developing, Pung-ok, which was very abundant in nitrogen, phosporic acid, glutamic acid, asp artic acid glutamine, asparagine, cystine, serine and sucrose but little in sugar, kalium and silicondioxide, was most susceptible to the disease. Jin-heung, resistant one, was opposite to Pung-ok in all aspects. As a result there was a correlation between the kind or amount of nutrients in rice plant and the disease attack. According to the amount of dressed fertilizer, the rice plant dressed in N-twofold and silicon dioxide none dressed had the larger amounts of total nitrogen, phosphoric acid, aspartic acid, glutamic acid and gldtamine but little in sugar and silicon dioxide contents. While in the rice plant dressed with N-ordinary and silicon fertilizer. the tendancy of disease occuring was low respectively. 2) The study on the cultivation of race T,C,N in the media added such organic acid as succinic acid, valeric acid, malic acid, ketoglutaric acid and such phenolic chlorogenic acid and salicylic acid was made. The result of which was as follow: ① There were no differences in growth of T,C,N type races. ② In the media added organic acid the growth was favorable. ③ In the media added chlorogenic acid the growth was promoted but in the one added salicylic acid the growth was inhibited. 3) The morphological changes of the infected waves alls the structure of hypha of blast which had penetrated into the leaf cell of Pung-ok, which was susceptible one to the disease, was observed by electric microscope. The following facts were found. ① In healthy leaves normal nucleus, mitohcondria, chloroplast, entoplasmic reticulum, vacuole and cytoplasm, especially round substances, were chearly observed by electron microspcope. ② In the infected leaves, the space of the nucleus double memberance was enlarged, and some of the nucleu walls were destroyed. The thinlayer structure of glanalamelae in lamela system was enlarged, somewhere spates were appeared while stromas were disappeared. In mitochondria the spaces of the double membrance were enlarged. ③ Cytoplasm of cell which were attacked by hypha was completely destroyed and the hypha lost their normal structure. ④ In the structure of hypha which penetrated into the cell wall of epidermis, nucleus, mitochondria, round ectoplasmic reticulum, vacuole and substances were clearly observed.

직렬 공진형 컨버터의 슬라이딩모드 전압제어

具本鎬,任成雲 慶一大學校 1992 論文集 Vol.8 No.1

This paper presents a method to control the output voltage of QSRC using sliding mode control theory. State equation is derived for the system that introduced control theory is applied to equivalent circuit of QSRC. The parameter of control rule selected in order to minimize the effect of load variation. By proposed control method, it was shown that output voltage of QSRC was controlled continuously. Robustness of proposed controller was pointed out via computer simulation and experiment.

강압형 컨버터의 퍼지제어

盧澈均,具本鎬,任成雲 慶一大學校 1993 論文集 Vol.9 No.1

This paper presents output voltage control method of buck converter using fuzzy control theory. Output voltage is obtained by duty ratio as output of the fuzzy control. By proposed fuzzy control system, it is good characteristics in the steady state and the transient. The system have robust output characteristics that have nothing to do with load variation from no load to full load. This feature is verified through the computer simulations.

Quantum Buck-Type Series Resonant Converter with Sliding Mode

Sung-Woon Im,Woo-Hyun Kwon,Gyu-Hyeong Cho 대한전기학회 1995 Journal of KIEE Vol.8 No.1

Characterization of Cryptic Plasmid of Multidrug - resistant Staphylococcus aureus SA2

( Sung Hwan Im,Sung Joon Yoon,Woo Koo Kim,Chul Kyo Shin,Dae Woon Lee,Kyung Ho Moon ) 한국미생물생명공학회 1996 Journal of microbiology and biotechnology Vol.6 No.2

EOTS에서의 동조자이로스코프의 동작모드에 따른 특성비교

임성운(Sung Woon Im),마진석(Jin Suk Ma),권우현(Woo Hyen Kwon) 제어로봇시스템학회 1997 제어·로봇·시스템학회 논문지 Vol.3 No.3

동조자이로스코프의 해석 및 응용 ( 전자광학추적기의 회전각속도 센서 )

임성운 ( Sung Woon Im ) 한국센서학회 1996 센서학회지 Vol.5 No.4

The basic principle and characteristics of a DTG(dynamically tuned gyroscope) are presented in this paper, which is used for the detection of disturbance and for the stabilization of gimbal. An accurate model of the rate mode DTG is proposed. This model has a resonance characteristics which is more similar to the characteristics of practical systems than the conventional 2nd order system model. Therefore, this model is applicable to the general rate mode gyroscope. Some problems at using DTG for a real electro optical tracking system are discussed and a solution is described.

유전알고리듬을 이용한 자기동조 제어기

任成雲,沈太銀 慶一大學校 1999 論文集 Vol.16 No.3

This paper presents the method for the automatic tuning of a design weighting polynomial parameter of a generalized minimum-variance stochastic self tuning controller which adapts to changes in the system parameters with time delays and noises using Genetic algorithm. The self tuning effect is achieved through the recursive least square algorithm at the parameter estimation stage and also through the Robbins-Monro algorithm at the stage of optimizing a design weighting polynomial parameters. The computer simulation results are presented to illustrate the procedure and toshow the performance of the control system.

회전자 파라미터를 보상한 유도전동기 간접 벡터제어

任成雲,朴哲佑,崔炳胎 慶一大學校 1999 論文集 Vol.16 No.3

It is proposed that the rotor time constant and inductance are compensated at the same time in the indirect vector control method of induction motor. The proposed scheme compensates the rotor time constant using the difference between Q-axis real stator current and estimated current that is calculated from terminal voltage and current, and compensates inductance using the difference between D-axis real stator flux and estimated stator flux in synchronous rotating reference frame. Although the rotor time constant and inductance vary at once, the proposed method compensates the rotor time constant and inductance with accuracy. In addition to, two variables cna be compensated not only at steady state condition, but also at transient state, where torque varies in rectangular pulse waveform. Therefore performance of vector control is improved highly and it is verified by experiment.