소양호에서 세균군집구조의 계절적·수직적 변화

김동주,홍선희,안태석 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

소양호에서 fluorescent in situ hybridization(FISH) 방법을 이용하여 세균군집의 계절과 수심에 따른 변화를 조사하였다. 이 방법에 사용된 oligonucleotide probe는 FUB338, ALF1B, BET42a, GAM42a와 CF probe였다. 조사 기간중 Proteobacteria α -group은 0.7~32.9% 이었으며 β -group은 1.0~25.8%, γ -group은 2.4~37.1%, cytophaga-Flavobacterium group은 4.7~23.6%의 분포를 나타내었다. 계절별로 살펴보면 규조류가 우점하는 봄철에는 γ -group이, 유기물의 농도가 낮고 수온이 높은 여름철에는 α -group이 우점하였으며, 남조류가 우점하는 가을철에 총세균수에 대한 Eubacterial group의 비율이 크게 감소하여 특정 group의 우점현상은 나타나지 않았다. 이처럼 소양호는 계절과 수심에 따라 군집구조가 변화하였으며, 특히 세균의 군집구조는 식물플랑크톤의 천이와 밀접한 관계를 보이는 것으로 나타났다. This study was conducted to investigate the change of bacterial communities with season and depth in Lake Soyang, Korea, using fluorescence in situ hybridization (FISH). The oligonucleotide probes used in this study were EUB338, ALF1b, GAM42a, and CF. The percentage of the Proteobacteria α -group ramged from 0.70 to 33%, the β -group from 1.0 to 26%, the γ -group from 2.4 to 37%, and Cytophaga and Flavobacteria groups from 4.7 to 24% during the study period (April to November, 1998). The γ -group was dominant in spring when Asterionella was dominant, and α -group was dominant in summer when the organic content was low and Dinobryon was dominant. However, a specific group was not dominant in fall when cyanobacteria group was dominant and the ratio of eubacteria to total bacteria was very low. Therefore, the bacterial communities in Lake Soyang changed with season and depth, which seems to be associated with the temporal succession of phytoplanktons.

한국인 만성 긴장형두통 환자에서 바이오피드백을 활용한 자율적 이완훈련의 효과

강은호,안주연,구문선,박주언,유범희 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.3

Objectives : We aimed to evaluate the efficacy of biofeedback-assisted autogenic training for chronic tension-type headache, and to determine the relationship among the changes in electromyography (EMG) activity, headache activity, and mood states according to the psychophysiological treatment. Methods : Chronic tension-type headache patients aged from 20 to 40 years (n=35) were randomized to the treatment group receiving biofeedback-assisted autogenic training (8 sessions) or the monitoring-only control group. EMG activities, headache index, and various psychological variables were examined. Results : We found greater treatment response rate (≥50% reduction in headache index) in patients with biofeedback-assisted autogenic training than in the monitoring group (61% vs. 18% ; χ²=6.882, df=1, p=0.01). There were no significant changes in the mean values of the pretreatment EMG activities across the sessions m either group (all p’s>0.1). Mood states including anxiety and depression improved over time in the both groups, with the improvements being more prominent in the treatment group. Moreover, the reduction in depression level predicted treatment outcome in terms of headache index (95% confidence interval : 0.272-0.966, p=0.039). Conclusion : These results show that biofeedback-assisted autogenic training is effective for the treatment of chronic tension-type headache in a Korean population. Changes in mood states may be closely associated with the clinical outcome in the treatment of chronic tension-type headache using biofeedback-assisted autogenic training.

문제행동 청소년을 위한 대인관계 집단치료 효과

이후경,안현주,김선재,윤성철,봉수연 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.5

Objectives : Fast change and confusion of the value system in society affect the identity formation of adolescents. So Adlescents' behavioral problems seem to be increasing yearly. It is known that the interpersonal group therapy is the most effective treatment modality among many group programs for adolescents with behavioral problems. Objectives of this study are as follows ; first, evaluate therapeutic effects of the interpersonal group therapy for adolescents with behavioral problems second, examine the therapeutic factors ; third, analyze the group process : fourth, orgamize the group therapy for adolescents with behavioral problems as an applicable treatment modality. The types of group therapy performed in this study are small, closed, homogeneous, outpatient, timelimited, and adolescent group. Methods : This study was carried out at one of the adolescents mental health services in Uiwang Mental Health Center, from April to June and from September to November in 2000. The subjects were 2nd grade students in K middle school with behavioral proplems. The experimental groups were composed of 4 groups including 2 male groups and 2 female groups (male 11, female 17), and the control groups matched the same conditions as the experimental groups (male 10, female 19). The group therapy was performed weekly in CA (club activity) time, and it took 60-70 minutes at one time. They met total 10 sessions including preparatory meeting and termination meeting. Before and after this program, self-rating scales (behavioral problems, aggression, and impulsivity) were applied for subjects, and the teacher rating scales (behavioral problems, aggression and impulsivity) were performed by teachers in charge. After each session, Yalom's 13 therapeutic factors scale was perfbrmed. At the last meeting, total assessment questionnaire was compleled. Results : The mean scores of all self-rating scales (behavioral problem, aggression, and impulsivity) were lower in the experimental groups (both male and female group) than the control groups, but not significant statistically except the behavioral problem scale of female students. The mean scores of all teacher rating scales (behavioral problem, aggression, and impulsivity) were lower in the experimental groups (both male and female group) in comparison with the control groups, all significant statistically. As faras the therapeutic factors are concerned ; first, the mean scores of all therapeutic factors in female students were higher than in male students and the mean scores of all therapeutic factors in the later half sessions were higher than the former half sessions ; second, catharsis and existential factor were high in all students and sessions ; third, identification with therapist factor was high in all students and sessions : fourth, during the later half sessions, interpersonal input was relatively high in male students and socializing technique was relatively high in female students. Its forthe total assessment questionnaires ; 100% of male students and 88.2% of female students reported that they were helped by this group therapy ; 100% of male students and 82.4% of female students reported that they would participate willingly if future opportunity of the same group therapy is given for them. Conclusion : Adolescents with behavioral problems who participated in this study showed the decrease of behavioral problems and the change of aggressive and impulsive attitudes in comparison with the control groups although they had some differences between male and female students. The group therapy with adolescents would be practiced more broadly and extensively though there are several accompanied problems including the difficulty of structuring, the deficit of motivation, and financial problem.

고도비만 환자에서 복강경 위소매절제술 후 발생한 누공의 내시경 치료 1예

류민선,심기남,조원영,김찬용,강현주,김미연,안소영,이윤표,조형원,정성애,이주호 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.2

Laparoscopic sleeve gastrectomy can reduce morbidity and mortality in patients with morbid obesity, but it can cause complications such as a gastrointestinal leak. A 30-year-old morbidly obese female who had type 2 diabetes mellitus and hypertension with estimated body mass index of 40.2 kg/m2 was admitted. Laparoscopic sleeve gastrectomy was performed. On postoperative day 19, a leak was suspicious on physical examination and radiologic findings. Conservative management was performed, but the patient was hemodynamically unstable and imminently septic. After laparoscopic drainage procedure, esophagogastroduodenoscopy was performed and revealed the fistula opening at staple line just below gastroesophageal junction. Fibrin tissue adhesive was injected around the fistula and the esophageal covered stent was inserted to cover the leak. At 14th days after stent insertion, the barium study confirmed no more leak. In this case, we experienced that the esophageal stent insertion with fibrin tissue adhesive injection may reduce recovery time of the fistula developed after laparoscopic sleeve gastrectomy

干拓地 浚渫土의 堆積特性에 關한 硏究

都德鉉,安成鎬,金善柱 건국대학교 산업기술연구원 1995 건국기술연구논문지 Vol.20 No.-

간척지 준설토의 퇴적 특성을 구명하기 위하여 충남 당진군 일원의 서남해안 干拓綜合開發事業 石門地區에서 준설공사를 하고 있는 排水閘門 引水路 浚渫土를 시료로 채취하여 시험분석한 결과를 요약하면 다음과 같다. 1) 本 試驗圃의 토질은 실트질 점토로서 浚渫 排砂管 吐出口 부위별 含泥率은 상부 7.36%, 중간부 18.85%, 하부 50.95%이고 평균값은 25.72%이다. 2) 堆積狀況은 浚渫 排砂管 吐出口를 중심으로 주위 약 30m까지는 粒子가 큰 모래와 점토 덩어리가 퇴적되고, 유속이 작은 원거리에서는 細砂 및 실트가, 精水狀態에서는 粘質土가 퇴적되므로 약간의 유실이 발생되어도 준설시의 유속을 크게 하면 粗粒子만 퇴적되어 양질의 지반을 얻을 수 있는 것으로 나타났다. 3) 浚渫土의 콘支持力 변화는 준설 3개월 후 준설 배사관을 중심으로 40∼60m 범위의 콘支持力(qc)은 2.0kgf/㎠, 一軸壓縮强度(qu)는 0.16~0.20kgf/㎠ 정도로서 超濕地에서 濕地로 支持力 증가 속도가 상당히 빠르고 60m 이상에서의 콘支持力(qc)은 0.5~1.0Kgf/㎠ 정도, 一軸壓縮强度(qu)는 0.02~0.06kgf/㎠로서 매우 연약한 超濕地임을 알 수 있었다. 4) 준설토의 강도증진은 자중압밀에 의한 강도증가보다 건조수축에 의한 강도 증진이 빠르고 크기 때문에 이 특성을 이용해서 浚渫盛土高를 20∼30㎝로 하여 건조수축시킨 후 반복적으로 浚渫盛土를 실시한다면 양호한 지반을 얻을 수 있을 것으로 생각된다. 5) 浚渫 排砂管 설치는 本管에 分岐될 지점에 직경이 다른 티(T형, Y형)를 가설해 두어 排砂管 移設에 필요한 경비를 절감할 수 있고, 排砂管 간격을 30m 전후로 배치하면 地盤改良에 필요한 공사비를 절감할 수 있는 것으로 나타났다. In order to study the deposition characteristics of dredged materials in the reclaimed area, deposition process of dredged materials and soil strength parameters in the Sukmoon Project area, which is located in the southeastern coast, have been analyzed with in-situ tests and laboratory research. The results obtained are as follows: Soil texture of the tested plot was silty clay, and the soil content of discharge flow by the part of pipe outlet was 7.36% at the upper part, 18.85% at the middle part and 50.95% at the lower part. Three different characteristics have been investigator from the observations on the relationship between the discharge flow velocity and soil particle size. Coarse sand and clusters of clay were deposited within the range of approximately 30meters from the outlet of discharge pipe where the flow velocity was relatively high. From 30meters to 60meters, finer particles were accumulated, and beyond this range, very fine clay particles were sedimented. So the results showed that the strength properties of dredged materials could be increased as the discharge velocity increased. In three months after the completion of dredging that was carried out within a range of 40∼60meters from the outlet and at a depth of 1.0m below the ground surface, the cone resistance(qc) and unconfined compression strength(qu) was 2.0kgf/㎠ and 0.16~0.20kgf/㎠, respectively, indicating that the increasing rate of bearing capacity is high. Beyond this range, qc and qu varied 0.5~1.0Kgf/㎠ and 0.02~0.06kgf/㎠, respectively, which is quite soft. Shear strength of the dredged materials increased evidently resulted from the shrinkage of drying rather than consolidation caused by the self-weight of deposition. Setting of the desilting scour pipe needs a "T" or "Y" type branch pipe of a different diameter to save the cost of setting. From the result of this study, it was found that if the desilting scour pipe is built with 30m spacing, the cost for land improvement can be saved.

β-Lactam 유도체의 N(1)-C(4) 위치에 한 개의 탄소를 도입한 입체 선택적 고리확장에 관한 연구

하진렬,오선주,김지아,신동수,안철진 昌原大學校 基礎科學硏究所 2003 基礎科學硏究所論文集 Vol.15 No.-

The base induced ring opening of β -1actam derivatives with LDA gave γ-lactam derivatives stereoselectively. The γ -1actam derivatives were formed stereoselectively depending on β-3 substituent of B-lactam derivatives.

한 중소도시의 청소년 약물사용 실태조사

이후경,김선재,윤성철,봉수연,안현주,박선영 대한신경정신의학회 2001 신경정신의학 Vol.40 No.1

-초록- 연구목적: 약물사용의 가능성이 가장 높은 시기는 십대 후반에서 이십대 초반의 청소년기로 알려져 있다. 또한 청소년기의 약뮬사용은 빠른 의존의 가능성, 심각한 약물남용으로 진행할 가능성, 약물로 인한 공격성의 증가 등으로 심각한 사회문제가 될 여지가 많다. 그러므로 저자들은 중소도시에서 청소년 약물사용의 예방 및 치료를 위한 정책을 마련하는데 기초가 될 수 있는 청소년 약물사용 실태를 조사하는 것을 목적으로혹시청소년상담실의 기금을 받아 본 연구를 시행하였다. 저자들이 세운 연구가 설은 다음과 같다. 첫째, 나이, 성(性)과 같은 개인적 요인에 따라 청소년의 약물사용에 차이가 있을 것이다. 둘째, 가정, 또래집단과 같은 환경적 요인에 따라 청소년의 약물사용에 차이가 있을 것이다. 셋째, 음주, 흡연, 비행과 약물사용과는 상화관련이 있을 것이다. 방법: ㅎ시 4개 중학교 학생 833명(51.6%), 3개 고등학교 학생 780명(48.4%), 성별은 남자(49.5%), 여자(50.5%), 총 1613명을 대상으로 하여 1999년 7월 12일부터 14일까지 3일간 설문조사를 실시하였다. 결과: 1) 술(59.4%), 담배(34.4%)의 경험이 가장 높았고, 의약품 중에는 진통제(33.1%), 흡입제(inhalants)중에는 본드(0.7%), 마약류 중에서는 히로뽕(0.4%)의 사용이 가장 많았다. 2) 술, 담배, 수면제, 각성제, 신경안정제의 사용경험이 중학생 보다 고등학생이 거의 두 배 이상 높았다. 3) 약물을 사용하지 않는 학생의 부모 화목도 점수가 약물을 사용하는 학생의 부모 화목도 점수보다 의미 있게 높은 것으로 나타났다. 4) 흡연 및 음주경험이 있는 학생의 친구들이 그렇지 않은 학생의 친구들에 비해 술, 의약품, 흡입제를더 많이 사용하였다. 5) 술, 담배, 약물을 사용하는 학생들이 비행 가능성이 높았다. 6) 술을 사용하게 되는 가장 많은 이유는 '친구들과 어울리기 위하여'(55.0%), 담배를 사용하게 되는가장 많은 이유는 '심심해서'(50.9%)이었다. 7) 술, 담배, 약물을 사용하면 다른 것을 사용할 가능성이 높은 것으로 나타났다. 결론: 한국의 청소년 약물사용경험은 이제까지의 연구와 본 연구를 종합하여 볼 때 지속적으로 증가하고 있다. 향후 약물사용을 줄이기 위해서는 국가적인 차원에서 약물사용 실태조사가 선행되어야 하고, 그 결과에 따른 적절한 대책의 수립과 정신과 의사들의 적극적인 참여가 필요하다. Objectives : The purpose of this research is to help solve adolescent issues involving substance uses, which may become begin to undergo many changes(i.e.practising various roles, thereby leading to independent life styles). More often than not, adolescent drug problems develop into social problems, chiefly behavioral imitations and heavy dependence on medicine. The authors conducted a survey of adolescent substance uses to develop preventive measures against the drug habit prevalent among young people, thereby formulating a policy to deal with problematic community. Funded by a Yuth Counselling Center in the city of H. the research was conducted on the following assumptions that there will be : Firstly, a comparison of drug uses by sex and age ; Secondly, variance of substance users by family background and peer group ; Thirdly, a combination of heavy drinking, smoking and delinquency resulting in the drug habit. Methods : the survey included a total of 1613 students during 3 days, 12 through 14, July, 1999. The subjects were 833 pupils(51.6$) from 4 middle schools and 780 students(48.4%) from 3 high schools in the city of H with a ratio of 49.5%(males) and 50.5%(females). Results : 1) Experience of substance use is most referable to drinking(59.4%) and smoking(34.4%) while given to analgesics(33.1%) among medicines, glue(0.7%) among inhalants and philophone(0.4%) among narcotics. 2) High school students found more than double the amount of smoking and drinking as well as uses of hypnotics, stimulants and sedatives by junior high school students. 3) Our resalt showed was significantly noted that non-users had a better relationship with their parents than most users. 4) Smoking and drinking problean could be more easily developing into adolescent substance uses. 5) Alcohol, cigarette, and drug users showed more misconducts than other adolescents. 6) Most adolescents believe theat drinking is necessary for making friends(55.0%), while smo-king is just for killing time(50.9%) 7) Drinking, smoking, and other substance users were found to develop into more proble-matic habits. Conclusion : The present survey and several others show that adolescent substance uses in Korea are on the rise. Therefore, extensive counter-measures will be necessary followed by nationwide epidemiological survey of the pending issue, with the psychiatrists participating in the campaign.

품행장애 청소년을 위한 외래집단정신치료 치료요인

이후경,김선재,차정화,봉수연,안현주 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 집단정신치료는 또래집단을 중요시하는 품행장애 청소년의 치료로서 매우 효과적인 것으로 알려져 있으나 한국에서는 아직 활성화되지 못하고 있다. 이에 따라 저자들은 품행장애 청소년에게 효과적으로 적용될 수 있는 폐쇄형, 동질적인, 시간제한(10회), 외래집단정신치료를 주 1회 실시하였다. 본 연구의 목적은 ①품행장애 청소년을 대상으로 집단정신치료를 시행하여 효과성을 평가하고, ②치료요인을 조사하여, ③ 집단정신치료를 품행장애 청소년에게 효과적이면서 효율적으로 적용될 수 있는 치료방법으로 정착시키는 데 있다. 대상 및방법: 본 연구는 1998년 10월붜 12월까지, 1999년 4월부터 6월까지 두차례에 걸쳐 실시하였다. 대상군은 품행장애 범주에 해당하는 H고교 2학년 여학생 총 18명(4명 도중 탈락), 대조군은 같은 범주에 해당하는 H고교 2학년 여학생 중 치료에 참여하지 않은 총 18명(4명 도중 탈락)이었다. 평가도구로는 프로그램 실시 전후에 자기보고식 비행척도, 공격성척도, 충동성척도을 시행하였고, 매 회기가 끝난 직후 Yalom의 13가지 치료요인척도, 집단치료 만족도 설문지를 시행하였으며, 종결모임시 총평가 설문지를 시행하였다. 결과: 치료요인의 변화를 집단발단단계에 따라 전후반기로 나누어 비교하였을 때, 전반기(1∼5회)에 상위를 차지한 치료요인은 순서대로 실존적인자, 보편성, 카타르시스, 리더와의 동일시, 구성원과의 동일시이었으며, 후반기(6∼10회)에 상위를 차지한 치료요인은 카타르시스, 실존적인자, 리더와의 동일시, 구성원과의 동일시, 구성원의 조언이었다. 전반기에 비해 후반기에 상대적으로 카타르시스, 구성원의 조언이 상승하였고 보편성은 하락하였다. 이타주의와 일차가족집단의 교정적 반복발달은 전후반기 모두 최하위를 기록하였다. 집단치료 만족도 설문에서 전반기는 45.7%, 후반기에는 83.3%가 도움받는 것으로 나타났고, 총평가 설문에서 85.7%가 기회가 있다면 다음에도 참여하고 싶다고 보고하였다. 결론: 시간제한 외래집단정신치료가 품행장애 청소년 에게 적용할 때 매우 효과적이면서도 만족도가 높은 것으로 나타났다. 청소년을 대상으로 외래집단정신치료를 시행하는 것은 구성상의 어려움, 동기부족, 기금부족 등, 여러 제반 문제가 있으나 확대 시행되어야 할 것으로 생각된다. Objectives: Group psychotherapy is known to be an dffective treatment program for adolescents who attach importance to peer relationship, but it is not activated in Korea. Therefore, researchers performed weekly the closed, homogeneous, time-limited(10 sessions), outpatient group psychotherapy for adolescents with conduct disorder. Objectives of this study were as follows ; first, we practiced the group psychotherapy for adolescents with conduct disorder and evaluated its effectiveness ; second, we examined the therapeutic factors which were used by participants ; third, we intended to settlee down the group psychotherapy for adolescents with CD as an effective and efficient treatment modality. Methods: This study was performed two times from October to December in 1998 and from April to June in 1999. It was sponsored by Youth Counselling Center in H city. The case group were composed of 15 female students who were second grade in H high school, and 4 students were dropped out. The control group were composed of 18 female students who were same grade in H high school and 3 students were dropped out. Before and after this program, self-report delinquency scale, aggression scale and impulsivity scale were asked to them. And just after each session, Yalom 13 therapeutic factors scale and satisfaction questionnaire for group psychotherapy were asked to them. And at last meeting, total assessment questionnaire was asked to them. Results: We compared the change of therapeutic factors between the former half sessions (1-5) and the later half session(6-10), and the results were as follows ; in the former half, the upper ranks of therapeutic factors were existential factor, universality, catharsis, identi- fication with leader, and identification with members in order. And in the later half, the upper ranks of therapeutic factors were catharsis, existential factor, identification with leader, identi - fication with members, and guidance of members in order. The rank of catharsis and guidance of members went upward and the rank of universality went downward when we compared the ranks in the former half to those in the later half. Altruism and family enactment were the lowest ranked in both half. The satisfaction questionnair for group psychotherapy showed to be helped by participants at the rate of 45.7% in former half, 83.3% in later half. The total assessment questionnaire showed the willingness of re-participation at the rate of 85.7% if future opportunity of group psychotherapy is given for them. Conclusions: The time-limited group psychotherapy for adolescents diagnosed by conduct disorder was very effectively practiced and the participants reported high level of the satisfaction. The outpatient group psychotherapy for adolescents would be expanded and practiced though there are several accompanied problems including the difficulty of structuring, the deficit of motivation, and financial problem.

만성정신질환자를 위한 장기외래집단정신치료 치료요인

이후경,이선영,윤성철,안현주 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

연구목적 : 만성정신질환자의 집단정신치료는 약물치료를 유지하면서 동시에 증상을 조절하고, 정서적인 갈등을 해결하며, 부적응적인 대인관계 문제를 향상시켜주는데 효과적이면서 접근이 용이한 치료방법이다. 이에 따라 저자들은 만성정신질환자에 효과적으로 적용될 수 있는 반개방형, 이질적인, 장기(시간무제한) 외래집단정신치료를 주 1회 실시하였다. 본 논문의 연구목적은 ① 만성정신질환자의 임상상황에 적용되는 집단정신치료의 치료원칙 및 운영방법을 간단히 소개하고, ② 참여자들이 도움 받은 것으로 보고한 치료요인을 집단발달단계에 따라 조사하고, 시행과정에 나타나는 특징을 분석하며, ③ 본 집단정신치료의 적절성과 타당성을 검증하여, 현재 한국 의료상황에 맞는 저비용, 고효율적인 모델로서 정착시켜 만성정신질환자의 장기외래치료를 보다 효과적으로 할 수 있는 기반을 마련하는데 있다. 대상 및 방법 : 본 연구는 1998년 1월부터 1996년 6월까지, 하남시 정신보건센터 주간보호에 출석하는 만성정신질환자들을 대상으로 하였다. 총 18개월 동안 참가자는 27명이었으나, 본 연구가 장기외래집단이기 때문에 최소한 참여기준기간을 6개월로 정하고, 최종적으로 6개월 미만 참여한 7명은 연구대상에서 제외시켜 총 20명(남자12명, 여자 8명)을 연구대상자로 선정하였다. 평가도구로는 대상자들에게 집단정신치료가 끝난 직후 13가지 치료요인척도를 작성하도록 하였고, 집단정신치료를 종결하는 환자나 본 연구를 종결짓는 시점에서 참여한 환자들을 대상으로 자기보고식 프로그램 총평가 설문지를 실시하였다. 결 과 : 13가지 치료요인척도의 순위는 형성기에 상위를 차지한 치료요인은 순서대로 실존적 인자, 치료자의 조언, 희망의 고취, 이타심, 카타르시스였고 갈등기에 상위를 차지한 치료요인은 순서대로 실존적 인자, 희망의 고취, 치료자의 조언, 카타르시스, 집단응집력이었다. 그리고 안정기에 상위를 차지한 치료요인은 순서대로 실존적 인자, 이타심, 일차가족집단의 교정적 반복발달, 대인관계학습, 카타르시스였다. 프로그램 총평가 설문 결과를 살펴보면, 집단정신치료에 대한 총평가에서 참여자의 90%가 도움을 받았다고 하였고, 80%가 집단정신치료를 다른 사람에게 적극적으로 권유하겠다고 하였으며, 75%가 재참여 의사를 보고하였다. 그리고 집단정신치료의 구성과 치료자에 관한 태도에서 매우 긍정적인 평가가 나왔다. 결 론 ; 장기외래집단정신치료는 저비용, 고효율적인 치료방법으로 만성정신질환자의 치료와 재활에 효과적으로 적용될 것으로 생각된다. 또한 치료자가 집단발달단계에 따라 다르게 나타나는 치료요인을 잘 알고 집단정신치료를 실시할 때 더 효과적일 수 있다. Objectives : The long-term outpatient group psychotherapy with the chronically ill is an effective, expedient therapeutic modality that makes patients maintain medication and improve their symptoms, help patients resolve emotional conflict, and corrects their maladaptive interpersonal relationship. Therefore, we performed weekly the semi-opened, heterogeneous, long-term(open-ended). Outpatient group psychotherapy to be applicable effectively for the chronically ill. The objectives of this study are as follows ; first, we introduce therapeutic principles and management methods of the group psychotherapy to be applicable in the clinical environment with the chronically ill ; second, we examine the therapeutic factors in each group development stage which are helped by participants, and analyze the characteristics showed in the process of study ; third, we verify the appropriateness and the validity of this group psychotherapy and then establish the base of the effective long-term outpatient therapy for the chronically ill that will be settled down as a applicable treatment modality economically and efficiently in the present medical environment of Korea. Methods : This study was performed in the subject of the chronically ill who attended at the day care program of Community Mental Health Center in Hanam city from January 1998 to June 1999. The participants were 27 patients, but we dropped 7 patients who attended under 6 months because of long-term group. So the last subjects were 20 patients(12 males and 8 females) who attended for 18 months. The 13 therapeutic factors scale was filled out by participants just after each session and the self-report total assessment questionnaire was performed at the time of study-termination and patient-termiation. Results : The ranks of 13 therapeutic factors scale are as follows : in the forming stage, the upper ranks of therapeutic factors were existential factor, guidance of therapists, instillation of hope, altruism, and catharsis in order. And in the storming stage, the upper ranks of therapeutic factors were existential factor, instillation of hope, guidance of therapists, catharsis, and group cohesiveness in order. Lastly in the norming stage, the upper ranks of therapeutic factors were existential factor, altruism, corrective recapitulation, interpersonal learning, and catharsis in order. According to the total assessment questionnaire about group psychotherapy reported by the participants 90% of them were helped, 80% of them had an idea to recommend this program to other patients actively, and 75% of them had an opinion to attend again this program if given for them. And they evaluated positively about the structure of this program and the therapist. Conclusions : The long-term outpatient group psychotherapy would be applicable effectively to the chronically ill as a economic, efficient therapeutic modality for rehabilitation. It is much more effective for therapist to perceive in detail the therapeutic factors in each group development stage when he perfoms the group psychotherapy.

출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리

박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1

복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.