Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear

Sun Joo LEE,Hae Jin JEONG,Ji Eun LEE,Jong Soo LEE,Ying Hua XUAN,Hyun-Hee KONG,Dong-Il CHUNG,Mee-Sun OCK,Hak Sun YU 대한기생충학열대의학회 2006 The Korean Journal of Parasitology Vol.44 No.4

Anti‐SOX1 Antibodies in Paraneoplastic Neurological Syndrome

Xuan Sun,Jiping Tan,Hui Sun,Yan Liua,Weiping Guan,Jianjun Jia,Zhenfu Wang 대한신경과학회 2020 Journal of Clinical Neurology Vol.16 No.4

Anti-Sry-like high mobility group box (SOX) 1 antibodies (abs) are partly characterized onconeural autoantibodies (autoabs) due to their correlation with neoplastic diseases. Anti- SOX1 abs are associated with various clinical manifestations, including Lambert-Eaton myasthenic syndrome (LEMS) and paraneoplastic cerebellar degeneration (PCD). However, the clinical characteristics of patients with anti-SOX1 abs have not been described in detail. This review systematically explores the reported patients with anti-SOX1 abs and analyzes these cases for demographic characteristics, clinical features, coexisting neuronal autoabs, neuroimaging findings, treatment, and clinical outcomes. In addition, considering that PCD is the most common paraneoplastic neurological syndrome and that the association between PCD and anti-SOX1 abs remains unclear, we focus on the presence of autoabs in relation to PCD and associated tumors. PCD-associated autoabs include various intracellular autoabs (e.g., anti-Hu, anti-Yo, anti-Ri, and anti-SOX1) and cell-surface autoabs (anti-P/Q-type voltage-gated calcium channel). Commonly involved tumors in PCD are small-cell lung cancer (SCLC), gynecological, and breast tumors. LEMS is the most common clinical symptom in patients with anti- SOX1 abs, followed by PCD, and multiple neuronal autoabs coexist in 47.1% of these patients. SCLC is still the predominant tumor in patients with anti-SOX1 abs, while non-SCLC is uncommon. No consistent imaging feature is found in patients with anti-SOX1 abs, and there is no consensus on either the therapy choice or therapeutic efficacy. In conclusion, the presence of anti-SOX1 abs alone is a potential predictor of an uncommon paraneoplastic neurological disorder, usually occurring in the setting of LEMS, PCD, and SCLC. The detection of anti-SOX1 abs contributes to an early diagnosis of underlying tumors, given the diversity of clinical symptoms and the absence of characteristic neuroimaging features.

Phylogenomic analysis and a systematic view of aquaporin family in rice

Minh Xuan Nguyen,Sun-Ok Moon,Ki-Hong Jung 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Global warming and climate changing nowadays are known as one of the most harmful factors concerning the yield of worldwide crop plants. To adapt with new challenges, a well-known strategy of plants is water-balance control. Aquaporins are a gene family of integral membrane proteins which play a central role in water transport regulation. By searching diverse databases, we expanded the number of rice aquaporin family from 33 to 37 genes. After that, the phylogenomic data integrating anatomical expression patterns consisting of 1150 affymetrix arrays and 209 Agilent 44K arrays, and stress responsible expression patterns were constructed and analyzed. The systemic overview of gene expression for rice aquaporin family is used to evaluate functional redundancy within this family and identify suitable target genes in response to water stress. Functional gene network mediated by water stress relating aquaporin genes also suggested a useful platform for further researches.

<i>Acanthamoeba</i>: Keratopathogenicity of isolates from domestic tap water in Korea

Jeong, Hae Jin,Lee, Sun Joo,Kim, Jeong Hwan,Xuan, Ying Hua,Lee, Keun Hee,Park, Sang Kyun,Choi, Sun Hee,Chung, Dong Il,Kong, Hyun Hee,Ock, Mee Sun,Yu, Hak Sun Elsevier 2007 Experimental parasitology Vol.117 No.4

<P><B>Abstract</B></P><P>In a previous study, we reported on the contamination rate of free living amoeba, including <I>Acanthamoeba,</I> isolated from contact lens storage cases (CLSC) and domestic tap water in Korea. In an effort to evaluate the potential kerato-pathogenicity of 5 isolates from CLSC and 17 isolates from domestic tap water, we have conducted an investigation into the morphological features, mitochondrial DNA (mtDNA) restriction fragment length polymorphism (RFLP) phenotypes, 18S rDNA sequences, and drug sensitivities of these isolates, and have compared the results with those of 20 amoebic keratitis (AK) isolates from Korea, as well as 14 reference strains. Cysts from 22 isolates obtained from CLSC and domestic tap water showed typical characteristics of morphological group 2. A total of three and five mtDNA RFLP patterns generated by EcoRI were found in 5 of the isolates from CLSC and 17 of the isolates from domestic tap water, respectively. The mtDNA RFLP patterns of four of the five isolates from the CLSC were found to be identical to those of the isolates from domestic tap water of students who had contaminated CLSC. The majority had mtDNA RFLP patterns identical to those of AK isolates in Korea. The results of 18S rDNA sequencing analysis were also shown to coincide with the results of mtDNA RFLP analysis. KA/WP12 was determined to be profoundly sensitive to chlorhexidine (MCC; 6.25μg/ml), and KAWP2 was the most sensitive strain to polyhexamethylene biguanide (PHMB) (MCC; 4.69μg/ml). Some difference in the cytopathic effects of isolates against human corneal epithelial cells was observed according to their mtDNA genotypes. In conclusion, domestic tap water may constitute a source of <I>Acanthamoeba</I> contamination of CLSC, and most isolates from CLSC and domestic tap water appear to be potentially keratopathogenic.</P>

Taxonomic Study of Spilomelinae (Lepidoptera, Pyraloidea, Crambidae) in Cambodia

Mu-Jie Qi,Yong-Don Ju,Xuan-Vi Le,Bo-Sun Park,Hee-Jo Lee,Min-Seon Kim,Yang-Seop Bae 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05

As one of the subfamilies of Crambidae, Spilomelinae comprises about 3767 species in the world (Solis and Maes, 2002), and this subfamily is characterized by the following characteristics: Chaetosema absent, antenna filiform, labial palpus and proboscis well developed, forewing with R3 and R4 stalk at base, or R2, R3 and R4 stalked, but R5 single, 2A and 1A form a loop; hindwing Sc+R1 stalked with Rs; male genitalia with gnathos absent or rudimentary, but uncus well developed, shape various. Cambodia is a country that bordered by Thailand, Laos, Vietnam and Gulf of Thailand in Southeast Asia. The total area is 181,035 km2. It is also a country with high biodiversity, of them, 212 mammal species, 536 bird species, 240 reptile species, 850 freshwater fish species, and 435 marine fish species, but only a few studies about the fauna of Lepidoptera there. Accordingly, to survey the diversity of Lepidoptera is of great significance for systematic study in Cambodia. In this research, we started our survey from 2009, up to now, we got numerous Pyraloidea from Cambodia, in this study, we identified 48 species belonging to 36 genera of Spilomelinae, most of them are reported for the first time in Cambodia, and some specimen could not be identified which we will report in future. All the materials examined come from the collection of University of Incheon.

Taxonomic Notes of Pyralinae (Lepidoptera, Pyraloidea, Pyralidae) in NE China

Mu-Jie Qi,Yong-Don Ju,Xuan-Vi Le,Bo-Sun Park,Hee-Jo Lee,Min-Seon Kim,Yang-Seop Bae 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

Pyralinae are one of the largest subfamilies belonging to Pyralidae, which comprises about 900 species of 217 genera in the world, and 150 species belonging to 32 genera are recorded in China (Li houhun, 2009). This subfamily are characterized by the following characteristics: head rounded, chaetosemata present, proboscis developed. Labial palpus porrect or upturned. Maxillary palpus small. Forewing colorful, from narrow to wide, antemedial line and postmedial line present and distinct. Hindwing color simple. Male genitalia with broad uncus, gnathos developed, slim, tip of gnathos sharp or hooked; tegument sclerotized, valve simple with various shape; juxta elliptical or rounded, aedeagus slender, cornutus present or absent. Female genitalia with small papillae anales, corpus bursae oval, signum present or absent. Liaoning Province is located in the southern part of NE China, the area of Liaoning is 146,900 km² and it is bounded on the south by the Yellow sea, on the southeast by North Korea, on the north by Jilin Province, and on the west by Inner Mongolia and Hebei Province. The mean annual temperature is 7-11℃, the mean annual precipitation is 600-1100 mm in northeast part, but 400 mm in west part. Liaoning Province is an important area for forest and agriculture, accordingly, to survey the diversity of Pyralinae is of great significance for systematic study in Liaoning Province. In this study, our materials examined come from the collection of Northeast forestry University from 2006-2010, and the collection of Incheon University from 2010-2012. Finally, we identified 11 species belonging to 8 genera, of them, 1 species are the first time reported in China, and 6 speices are the first time reported in Liaoning Province.

Tumor Location Causes Different Recurrence Patterns in Remnant Gastric Cancer

Sun Bo,Zhang Haixian,Wang Jiangli,Cai Hong,Xuan Yi,Xu Dazhi 대한위암학회 2022 Journal of gastric cancer Vol.22 No.4

Purpose Tumor recurrence is the principal cause of poor outcomes in remnant gastric cancer (RGC) after resection. We sought to elucidate the recurrent patterns according to tumor locations in RGC. Materials and Methods Data were collected from the Shanghai Cancer Center between January 2006 and December 2020. A total of 129 patients with RGC were included in this study, of whom 62 had carcinomas at the anastomotic site (group A) and 67 at the non-anastomotic site (group N). The clinicopathological characteristics, surgical results, recurrent diseases, and survival were investigated according to tumor location. Results The time interval from the previous gastrectomy to the current diagnosis was 32.0±13.0 and 21.0±13.4 years in groups A and N, respectively. The previous disease was benign in 51/62 cases (82.3%) in group A and 37/67 cases (55.2%) in group N (P=0.002). Thirty-three patients had documented sites of tumor recurrence through imaging or pathological examinations. The median time to recurrence was 11.0 months (range, 1.0–35.1 months). Peritoneal recurrence occurred in 11.3% (7/62) of the patients in group A versus 1.5% (1/67) of the patients in group N (P=0.006). Hepatic recurrence occurred in 3.2% (2/62) of the patients in group A versus 13.4% (9/67) of the patients in group N (P=0.038). Patients in group A had significantly better overall survival than those in group N (P=0.046). Conclusions The tumor location of RGC is an essential factor for predicting recurrence patterns and overall survival. When selecting an optimal postoperative follow-up program for RGC, physicians should consider recurrent features according to the tumor location. Purpose Tumor recurrence is the principal cause of poor outcomes in remnant gastric cancer (RGC) after resection. We sought to elucidate the recurrent patterns according to tumor locations in RGC. Materials and Methods Data were collected from the Shanghai Cancer Center between January 2006 and December 2020. A total of 129 patients with RGC were included in this study, of whom 62 had carcinomas at the anastomotic site (group A) and 67 at the non-anastomotic site (group N). The clinicopathological characteristics, surgical results, recurrent diseases, and survival were investigated according to tumor location. Results The time interval from the previous gastrectomy to the current diagnosis was 32.0±13.0 and 21.0±13.4 years in groups A and N, respectively. The previous disease was benign in 51/62 cases (82.3%) in group A and 37/67 cases (55.2%) in group N (P=0.002). Thirty-three patients had documented sites of tumor recurrence through imaging or pathological examinations. The median time to recurrence was 11.0 months (range, 1.0–35.1 months). Peritoneal recurrence occurred in 11.3% (7/62) of the patients in group A versus 1.5% (1/67) of the patients in group N (P=0.006). Hepatic recurrence occurred in 3.2% (2/62) of the patients in group A versus 13.4% (9/67) of the patients in group N (P=0.038). Patients in group A had significantly better overall survival than those in group N (P=0.046). Conclusions The tumor location of RGC is an essential factor for predicting recurrence patterns and overall survival. When selecting an optimal postoperative follow-up program for RGC, physicians should consider recurrent features according to the tumor location.

FeB와 TiH₂ 혼합분말의 유성볼밀 및 후속열처리에 의한 Fe-TiB₂ 나노복합분말 in situ 제조

( Xuan-khoa Huynh ),배선우 ( Sun-woo Bae ),김지순 ( Ji Soon Kim ) 대한금속재료학회(구 대한금속학회) 2017 대한금속·재료학회지 Vol.55 No.1

Fe-TiB₂ powder was synthesized in-situ by the planetary ball milling and subsequent heat-treatment of an iron boride (FeB) and titanium hydride (TiH₂) powder mixture. Mechanical activation of the (FeB+TiH₂) powder mixtures was observed after a milling time of 3 hours at 700 rpm of rotation speed, but activation was not the same after 1 hour milling time. The particle size of the (FeB+ TiH₂) powder mixture was reduced to the nanometer scale, and each constituent was homogeneously distributed. A sharp exothermic peak was observed at a lower temperature (749 ℃) on the DSC curves for the (FeB+TiH₂) powder mixture milled for 3 hours, compared to the one milled for 1 hour (774 ℃). These peaks were confirmed to have resulted from the formation reaction of the TiB2 phase, from Ti and B elements in the FeB. The Fe-TiB₂ composite powder fabricated in situ exhibited only two phases of Fe and TiB₂ with homogeneous distribution. The size of the TiB2 particulates in the Fe matrix was less than 5 nm. (Received July 22, 2016; Accepted July 25, 2016)

ANXA2 Regulates the Behavior of SGC-7901 Cells

Sun, Meng-Yao,Xing, Rui-Huan,Gao, Xiao-Jie,Yu, Xiang,He, Hui-Min,Gao, Ning,Shi, Hong-Yan,Hu, Yan-Yan,Wang, Qi-Xuan,Xu, Jin-Hui,Hou, Ying-Chun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

ANXA2, a member of the annexin family, is overexpressed and plays important roles in tumor development. However, the significance of ANXA2 expression in gastric carcinoma has not been clarified.To elucidate its roles in growth of gastric cancer, ANXA2 expression in SGC-7901 cells was inhibited with a designated siRNA, then cell proliferation, cell cycling, apoptosis and motility were determined by MTT assay, flow cytometry, Hoechst 33342 staining and wound healing assay, respectively. To further assess the behavior of ANXA2 deleted SGC-7901 cells, changes of microstructures were observed under fluorescence microscopy, laser scanning confocal microscopy and electron microscopy. We found that inhibition of ANXA2 expression caused cell proliferation to decrease significantly with G1 arrest, motility to be reduced with changes in pseudopodia/filopodia structure and F-actin and ${\beta}$-tubulin expression, and apoptosis to be enhanced albeit without significance. At the same time, ANXA2 deletion resulted in fewer pseudopodia/filopodia, non-stained areas were increased, contact inhibition among cells reappeared, and expression of F-actin and ${\beta}$-tubulin was decreased, with induction of polymerized disassembled forms. Taken together, these data suggest that ANXA2 overexpression is important to maintain the malignancy of cancer cells, and this member of the annexin family has potential to be considered as a target for the gene therapy of gastric carcinoma.

Finite element simulation of ductile fracture mechanisms in AlSi10Mg processed by laser powder bed fusion

Xuan Minh Nguyen(유엔션민),Dang Hoang Cong(당황공),Minh Tien Tran(쩐민띠엔),A Ra Jo(조아라),Sun-Kwang Hwang(황선광),Dong-Kyu Kim(김동규) 한국소성·가공학회 2024 한국소성가공학회 학술대회 논문집 Vol.2024 No.5