High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

Sun Di,Mou Shan,Chen Lifeng,Yang Jie,Wang Rongrong,Zhong Aimei,Wang Wei,Tong Jing,Wang Zhenxing,Sun Jiaming 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background: Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been found to have a great potential for soft tissue repair due to various biological functions, including pro-angiogenesis and low immunogenicity. However, the low yield and heterogeneity of MSC-EVs limited their clinical transformation. This study was designed to develop a novel adipose-derived stem cell engineered nanovesicles (ADSC-NVs) with high production and explore its pro-angiogenetic effect and application in adipose tissue regeneration. Methods: Adipose-derived stem cell-derived extracellular vesicles (ADSC-EVs) were isolated from an EVs-free culture medium for human ADSCs (hADSCs). ADSC-NVs were prepared by sequentially extruding ADSCs followed by iodixanol density gradient ultracentrifugation and were compared with ADSC-EVs in morphology, size distribution, protein contents and yield. The pro-angiogenetic effect of ADSC-NVs in different doses (0, 5, 20 and 80 μg/mL) in vitro was determined using transwell assay, tube formation assay, western blot and qRT-PCR. In vivo, BALB/c nude mice were administered injection of a mixture of fat granules and different dose of ADSC-NVs and grafts were harvested at 12 weeks post-transplantation for further analysis. By analyzing the weight and volume of grafts and histological evaluation, we investigated the effect of ADSC-NVs in vessel formation and adipose tissue regeneration. Results: Our results showed yield of purified ADSC-NVs was approximately 20 times more than that of ADSC-EVs secreted by the same number of ADSCs. In vitro, both ADSC-NVs and ADSC-EVs exhibited a dose-dependent proangiogenetic effect, despite their distinct miRNA profiles. These effects of ADSC-NVs may be mediated by enriched miR-21-5p via PTEN inhibition and PI3K/p-Akt signaling activation. Furthermore, after a mixed injection of ADSC-NVs, vessel formation and adipose regeneration were observed in vivo in fat implants. Conclusions: Our study developed a potent alternative of ADSC-EVs. ADSC-NVs have a high pro-angiogenesis potential and can be used as cell-free therapeutic biomaterials in soft tissue regeneration.

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Sun, Chuanxi,Zhu, Tianyi,Zhu, Yuwei,Li, Bing,Zhang, Jiaming,Liu, Yixin,Juan, Changning,Yang, Shifa,Zhao, Zengcheng,Wan, Renzhong,Lin, Shuqian,Yin, Bin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.3

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were "protein processing in endoplasmic reticulum," "retinol metabolism," and "glycine, serine, and threonine metabolism." Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Chuanxi Sun,Tianyi Zhu,Yuwei Zhu,Bing Li,Jiaming Zhang,Yixin Liu,Changning Juan,Shifa Yang,Zengcheng Zhao,Renzhong Wan,Shuqian Lin,Bin Yin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.4

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10 mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were “protein processing in endoplasmic reticulum,” “retinol metabolism,” and “glycine, serine, and threonine metabolism.” Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

Optimization design of graphene with adjustable hydrophilic properties by finite elements analysis

Shixun Sun,Jiaming Song,Naiyu Cui,Xinyue Lu,Xuehan Li,Heng Zhang,Heng Bo Jiang 대한치과재료학회 2021 대한치과기재학회 학술대회 Vol.2021 No.4

Generalized Likelihood Ratio Test For Cyclostationary Multi-Antenna Spectrum Sensing

( Guohui Zhong ),( Jiaming Guo ),( Daiming Qu ),( Tao Jiang ),( Jingchao Sun ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.8

In this paper, a generalized likelihood ratio test (GLRT) is proposed for cyclostationary multi-antenna spectrum sensing in cognitive radio systems, which takes into account the cyclic autocorrelations obtained from all the receiver antennas and the cyclic cross-correlations obtained from all pairs of receiver antennas. The proposed GLRT employs a different hypotheses problem formulation and a different asymptotic covariance estimation method, which are proved to be more suitable for multi-antenna systems than those employed by the Dandawate-Giannakis algorithm. Moreover, we derive the asymptotic distributions of the proposed test statistics, and prove the constant false alarm rate property of the proposed algorithm. Extensive simulations are conducted, showing that the proposed GLRT can achieve better detection performance than the Dandawate-Giannakis algorithm and its extension for multi-antenna cases.

우유에서 장출혈성 대장균과 캠필로박터균의 행동예측 모델 개발 및 정량적 미생물 위해성 평가 연구

동쟈밍(Dong Jiaming),민경진(Kyung Jin Min),서건호(Kun Ho Seo),윤기선(Ki Sun Yoon) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.5

본 연구는 일반우유와 무지방우유에서 장출혈성 대장균과 캠필로박터 제주니의 행동예측모델을 개발하고, 미생물학적 안전관리를 위한 기준의 적절성 평가를 위해 정량적 위해성평가를 수행하였다. 시중 마트에서 유통 판매되고 있는 일반우유(n=195)에서 장출혈성 대장균과 캠필로박터 제주니의 오염실태를 모니터링한 결과 모든 제품에서 장출혈성 대장균과 캠필로박터 제주니는 검출되지 않아 초기 오염도는 각각 -3.94 log CFU/mL로 동일하게 추정되었다. 장출혈성 대장균은 7℃ 이상의 온도에서 성장하였고, 캠필로박터 제주니는 4-25℃ 온도의 우유에서 사멸하였다. 우유에서 1차 모델에서 얻은 parameter를 사용하여 장 출혈성 대장균은 2차 성장모델을 캠필로박터 제주니는 2차 사멸예측모델을 개발하였다. 일반우유의 섭취패턴은 식품의약품안전처(2015) 연구에서 수행한 “50대 주요 축산식품의 섭취량 및 섭취패턴조사” 결과를 바탕으로 @RISK 프로그램을 활용하여 하루에 일반우유의 1회 섭취를 통하여 장출혈성 대장균과 캠필로박터 제주니에 의한 식중독 발생 확률을 추정하였다. 추정 결과 1일 1회 일반우유 섭취로 장출혈성 대장균과 캠필로박터 제주니로 인한 평균 식중독 발생 확률은 각각 5.70×10<SUP>−5</SUP>, 9.86×10<SUP>−9</SUP> 것으로 확인되었다. 본 연구에서 정량적 위해평가를 통해 일반우유에서 장출혈성 대장균과 캠필로박터 제주니의 위해수준을 산출한 결과 일반우유에서 장출혈성 대장균의 식중독 발생 가능성이 상대적으로 높으므로 우선관리 대상임을 알 수 있었고, 우유제조업체에서 교차오염 방지, 살균온도/시간 관리, 유통온도, 가정에서 온도관리 등이 매우 중요할 것으로 사료된다. We prepared the growth and survival models of enterohemorrhagic Escherichia coli (EHEC) and Campylobacter jejuni in milk as a function of temperature and assessed the microbiological risks associated with the consumption of whole milk. EHEC and C. jejuni were not detected in whole milk (n=195) in the retail market. The minimum growth temperature of EHEC in milk was 7℃. The lag time of EHEC in whole milk was longer than that in skim milk. The survival ability of C. jejuni in milk was better at 4℃ than at 10℃. Lower delta values were observed in whole milk than in skim milk, indicating that C. jejuni survived better in skim milk. The probability of foodborne illness from whole milk consumption was 5.70×10<SUP>−5</SUP> for EHEC and 9.86×10<SUP>−9</SUP> for C. jejuni. Sensitivity analysis results show that the market temperature of EHEC and the dose-response model of C. jejuni are correlated with the probability of foodborne illness.

Adaptive Pinning Synchronization of Complex Networks with Partial Delay and Noise Coupling

Wang Li,Yue Qiao,Jiaming Wu,Hongjun Shi,Yongzheng Sun 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.1

In this paper, we investigate the adaptive pinning synchronization of complex networks with partial delay and noise coupling. First, more realistic complex networks with partial delay and noise perturbation are established. Combining the advantages of the adaptive control method and pinning control technology, a novel coupling is designed to realize the synchronization. Several sufficient criteria for almost sure outer synchronization are developed based on the LaSalle invariance principle for stochastic differential equations. Finally, numerical simulations are performed to verify the effectiveness of the proposed synchronization scheme.

Discovery of the Early Paleozoic Akechukesai high-Mg diorites in the western segment of East Kunlun Orogenic Belt and its constraints on the mechanism of break-off from Proto-Tethys oceanic subducted slab

Chao Wang,Fengyue Sun,Dongwei Liu,Lei Zuo,Tuofei Zhao,Jiaming Yan 한국지질과학협의회 2022 Geosciences Journal Vol.26 No.1

High-Mg andesites (HMAs) and their cognate intrusive rocks constitute volumetrically very small proportions of the total earth, and are mainly distributed along the edges of convergent plates. Petrogenetic studies can provide possible solutions for discrepancies in the geodynamics and subduction zone evolution. This paper presents the first ever reports of the newly discovered high-Mg diorite in Akechukesai area, the western part of the East Kunlun Orogenic Belt, and provides a reference for the evolutionary history and subduction mechanism of the Proto-Tethys Ocean. Akechukesai high-Mg diorites yielded a weighted mean zircon U-Pb dating age of 427.3 ± 2.3Ma (Middle Silurian). Results of the geochemical analyses show that the high-Mg diorites were high-K calc-alkaline series with the SiO2 content ranging 50.40 to 55.41 wt%. They are characterized by high values of Mg# (67–77), high MgO (6.92–10.58 wt%), TiO2 (0.53–0.87 wt%), Cr (286–615 ppm), Ni (61–124 ppm), Ba (570–927 ppm) contents, and low FeOtotal/MgO ratios (0.54–0.89). Furthermore, they exhibit nearly flat right-declined rare-earth element (REE) patterns with slight LREE enrichment. The samples are enriched in large ion lithophile elements (e.g., Ba, Rb, and Th) and depleted in high field strength elements (e.g., Ta, Nb, and Ti). These geochemical features are analogous to the sanukitic high-Mg andesites. The mean value of the initial εHf(t) is –1.3, indicating that the source is enriched mantle. The values of Rb/Cs, Ba/La, and La/Sm ratios suggest that subducting sediments formed an important component of the magmatic source. The presence of water-bearing minerals such as amphibole and biotite indicate a water-rich and oxygen-rich primitive magma system. Petrogenetic analysis indicates that the Akechukesai high-Mg diorites probably formed by melts and aqueous fluids produced from partial melting of the subducting sediments interacting with mantle peridotites. We hypothesize that, after the closure of the Proto-Tethys Ocean Basin in the Middle Silurian, the deep subducted slab broke-off and formed a slab window, asthenospheric material upwelled heating the subducting sediments and causing them to melt. Thus, we suggest that the emplacement of the Akechukesai high-Mg diorites mark the commencement of post-collisional magmatism.

Changes in Enteric Neurons of Small Intestine in a Rat Model of Irritable Bowel Syndrome with Diarrhea

( Shan Li ),( Guijun Fei ),( Xiucai Fang ),( Xilin Yang ),( Xiaohong Sun ),( Jiaming Qian ),( Jackie D Wood ),( Meiyun Ke ) 대한소화기기능성질환·운동학회 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.2

Background/Aims Physical and/or emotional stresses are important factors in the exacerbation of symptoms in irritable bowel syndrome (IBS). Several lines of evidence support that a major impact of stress on the gastrointestinal tract occurs via the enteric nervous system. We aimed to evaluate histological changes in the submucosal plexus (SMP) and myenteric plexus (MP) of the distal ileum in concert with the intestinal motor function in a rat model of IBS with diarrhea. Methods The rat model was induced by heterotypic chronic and acute stress (CAS). The intestinal transit was measured by administering powdered carbon by gastric gavage. Double immunohistochemical fluorescence staining with whole-mount preparations of SMP and MP of enteric nervous system was used to assess changes in expression of choline acetyltransferase, vasoactive intestinal peptide, or nitric oxide synthase in relation to the pan neuronal marker, anti-Hu. Results The intestinal transit ratio increased significantly from control values of 50.8% to 60.6% in the CAS group. The numbers of enteric ganglia and neurons in the SMP were increased in the CAS group. The proportions of choline acetyltransferase- and vasoactive intestinal peptide-immunoreactive neurons in the SMP were increased (82.1 ± 4.3% vs. 76.0 ± 5.0%, P = 0.021; 40.5 ± 5.9% vs 28.9 ± 3.7%, P = 0.001), while nitric oxide synthase-immunoreactive neurons in the MP were decreased compared with controls (23.3 ± 4.5% vs 32.4 ± 4.5%, P = 0.002). Conclusions These morphological changes in enteric neurons to CAS might contribute to the dysfunction in motility and secretion in IBS with diarrhea. (J Neurogastroenterol Motil 2016;22:310-320)