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서석기(Sug Kee Suh),김학신(Hag Sin Kim),조상균(Sang Kyun Jo),오영진(Young Jin Oh),김수동(Soo Dong Kim),장영선(Young Sun Jang) 한국콩연구회 1995 韓國콩硏究會誌 Vol.12 No.1
As a primary study of soybean breeding for high quality soybean sprouts, this experiment was conducted to investigate the growing characteristics with 4 cultivars of which seed weight are different. Soybean seeds were soaked in under ground water and Saerona liquid(60 times) which is No.4 complex fertilizer for 4 hours and they were cultured under 4 different temperature conditions (15±1, 20±1, 25±1, 30±1℃) Hypocotyl length, root length and root hair number of soybean sprout are increased by the cultural temperature rise. But soybean sprout quality was not good by increase of the root length, hair number and putrefied seeds at 30±1℃. Yield ratio was high with small seed. So to grow high quality soybean sprouts, optimum culturing temperature was 20∼25±1℃ with small seed.
저장기간에 따른 나물콩 품종의 발아력 및 콩나물 특성 1 . 저장기간에 따른 나물콩 품종의 발아력
서석기(Sug Kee Suh),김경호(Kyung Ho Kim),김학신(Hag Sin Kim),오영진(Young Jin Oh),김수동(Soo Dong Kim),장영선(Young Sun Jang) 한국콩연구회 1995 韓國콩硏究會誌 Vol.12 No.2
Using the sprout soybean varieties and breeding lines in National Honam Agricultural Experiment Station, the aspects of water uptake patterns and germinability were tested to get the information for varieties improvement. The aspects of water uptake patterns was active absorption upto six hours of initial stage but very inactive after that stage. The longer period of storage, the higher ratio of water uptake patterns. The initial germination ratio was higher in shock period of storage, the number of initial germination seeds were higher in high temperature (25℃) than low temperature (15℃). The germination speed was also faster in shorter period of storage, under high temperature stored seeds as three years above, and under low temperature condition as two years below, respectively. Uniformity of germination was better in low temperature than high. The shorter period of storage was more uniform. The days consumed for germination was taken a longer days in low temperature than high temperature and longer period of storage. The final germination ratio was higher in low temperature than high, but there was no difference among varities.
저장기간에 따른 나물콩 품종의 발아력 및 콩나물 특성 2 . 저장기간에 따른 나물콩 품종의 콩나물 특성
서석기(Sug Kee Suh),김경호(Kyung Ho Kim),김학신(Hag Sin Kim),오영진(Young Jin Oh),김영진(Young Jin Kim),박호기(Ho Ki Park),장영선(Young Sun Jang) 한국콩연구회 1996 韓國콩硏究會誌 Vol.13 No.1
This experiment was conducted to know the offect of seed storage period on the germinability and soybean - sprout (ss) characteristics using varieties for sprout soybean and breeding lines in National Honam Agricultural Experiment Station (NHAES) at NHAES in 1995, Soybean - sprout cultured for five days in incubator at 25℃. The yield of SS showed higher mainly due to long hypocotyl and root length when the sprout soybean seeds were stored for short period, while poor growth rate and high putrefaction were observed with increasing storage period. Moreover, The stored seeds for four to five years showed brownish of hypocatyl color as well as poor germination. As a results, the stared seeds for 3 years at least can be utilized for the production of SS but the seeds stored less than 2 years and with genotypes of light seed weight are considered more desirable in terms of productivity of SS and quality.
김경호(Kyong Ho Kim),오영진(Young Jin Oh),서석기(Sug Kee Suh),김학신(Hag Sin Kim),김태수(Tae Soo Kim),이미자(Mi Ja Lee),박호기(Ho Ki Park),서득룡(Duck Yong Suh),여읍동(Up Dong Yeo) 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.1
To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as meterial part and size of immature seed, 2,4-D pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon, and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up tp 23%) of somatic embryogenesis was obtained from the immature cotyledon, followed by embryonic axis and immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25%) of embryogenesis was obtained from 3-4 mm length of the seed after 25 days of flowering. The optimum concentration of 2,4-D for embryogenesis was 10mg/l. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tip from germinating somatic embryos, they were cultured on MS medium containing 2 mg/L IBA and 1 mg/L BAP, After then somatic embryos with one and three cotyledons were transferred to the plant growth regulator free medium. The medium exhibited the higher rate (50%) of development than the multiplication medium. To select the section with shoot formation ability, the calli and shoot formation from three sections of 5-days-seedings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section and the cotyledon section. The shoot formation rates and shoot number of the four excised sections of the first leaf were aldo investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and number (3-4/ explants) than those in other two explants. Effects of cytokinins on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0mg/L) exhibited the highest in shoot formation rate (94%) and combination (1: 1, v/v) of zeatin (1.0mg/L) and IAA (1.0 mg/L) eshibited the highest in shoot formation rate (96%) and numbers (16/explant), twice more than zeatin alone (1.0 mg/L). The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at variois concentrations. The highest root formation (8/shoot) was achieved on the medium supplementes with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.
Sang Kyun Cho(趙湘均),Young Jin oh(吳永鎭),Sug Kee Suh(徐錫埼),Hak Sin Kim(金學信),Soo Dong Kim(金洙東),Young Sun Chang(張榮宣),Kyeong Gu Choi(崔京求) 한국육종학회 1995 한국육종학회지 Vol.27 No.2
Improvement of sprout soybeans could be accomplished by maintenance and collection of varieties which have characteristics of small seed, disease resistance and high yielding ability. This research was conducted to investigate morphological and ecological characters for 102 sprout soybean varieties collected in Chonnam and Chonbuk area, and donated by Sprout Soybean Association and Genetic Engineering Research Institute. The sprout soybean collections contained 85% purple flower color, 76% ring-type leaf, 68% brown trichome, 57% elliptical-type seed, 69% yellow seed color and 44% brown hilum color. Days to flowering ranged from 53 to 79 days and growth duration averaged 137 days, with the range of 119~149 days. Stem length ranged from 52㎝ to 165㎝ and 100-seed weight distributed into 4.9~13.3g. The grain yield revealed large range of 0.53~4.18MT/ha. Crude protein and lipid contents was ranged 35.8~48.7% (average 40.9%), and 19.1~’21.2% (average 20.1%), respectively. Correlation coefficients between grain yield and pod numbers was significantly positive, while that between 100-seed weight and pod numbers was significantly nagative. Crude protein contents was significantly associated with growth duration but negatively correlated with 100-seed weight and yield. Crude lipid contents was negatively correlated with growth duration but positively associated with 100-seed weight.