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Jeerun Kingkaew,Suchata Kirdponpattara,Neeracha Sanchavanakit,Prasit Pavasant,Muenduen Phisalaphong 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.3
Bacterial cellulose-chitosan (BC-C) films weredeveloped by immersing purified BC pellicles in 1.5 ~2.0% (w/v) acetic acid solutions containing chitosan ofvarying molecular weights. Effects of different molecularweight of chitosan on physical, biological and antimicrobialproperties of the composite films were investigated. Thecumulative chitosan absorption capacities with Mw of141,000, 199,000, and 263,000 were 38.43, 24.65, and23.89 mg/cm3 of dry BC film, respectively. The cumulativerelease profiles of chitosan from the films stronglydepended on molecular weight of chitosan and pH ofsolution. The order of release of chitosan from the BC-Cfilms was dependent on molecular weight as follows: Mw141,000 > Mw 199,000 > Mw 263,000. All BC-C filmsshowed the antimicrobial abilities against Staphylococcusaureus and Aspergillus niger but had no inhibitory effecton the growth of Escherichia coli. The BC-C filmssupported for adhesion, spreading and proliferation of bothhuman skin keratinocytes and fibroblasts. The antibacterialactivity against S. aureus of the BC-C with the highest Mwchitosan (263,000) was higher than those of the others. Onthe other hand, the BC-C films with the lowest Mw chitosan(141,000) promoted the growth of human skin cells morethan those of the others.
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