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RISS 인기검색어
- 검색키워드
- 저자 : Stephen L. Love (검색결과 2 건)
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In Vitro Screening of Potato Genotypes for Resistance to Common Scab Using Thaxtomin A
Dong-Man Khu,Stephen L. Love,Hwang-Sung Mo,Kui-Hua Li,Hak-Tae Lim 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.4
Thaxtomin A is a bio-toxin produced by Streptomyces scabies and is thought to be responsible for visible tuber symptoms associated with the common scab disease. It was hypothesized that response of in vitro plantlets to the toxin may be related to tuber reaction in the field. A population of 100 genotypes originating from a resistant x susceptible cross was evaluated for both in-vitro thaxtomin response and field occurrence of common scab on the tubers. The shoot height of plantlets grown on the medium containing thaxtomin A was used as a measure of the tolerance of each genotype. The surface area infected index (SAII) of each genotype was measured and used as gauge of resistance level of each genotype in the field. The correlation coefficient between in vitro shoot height of plantlets, grown on media containing 1.0 mgㆍL<SUP>-1</SUP> of thaxtomin A, and SAII of each genotype was highly significant (r = - 0.656). in vitro screening of potato germplasm using thaxtomin shows promise as a method to select for resistance to common scab.
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Kui-Hua Li,Eung-Jun Park,Hoi-Seon Lee,Dong-Man Khu,Stephen L. Love,Hak-Tae Lim 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.3
Potato is a valuable source of antioxidants in the human diet and their genetic variability exists for antioxidant activity. This study was carried out to define selection criteria for potato varieties and breeding clones with high antioxidant levels as measured by 1,1-diphenyl-2-picryl-hydrazyl methods. There were significant differences in antioxidant activities among potato genotypes. Radical scavenging activities (RC??) of 29 potato genotypes ranged from 64.8 to 493.6 ㎍ㆍmL?¹. We further analyzed the antioxidant activities in three different sections including cortex, perimedullary, and pith tissue were separately measured for five selected potato varieties. At least, two-fold higher antioxidant activity for cortex tissue (5 ㎜-thick of periderm tissue with skin) was found in all five ‘Valley’ potatoes, compared to that in ‘Atlantic’. Very low antioxidant activity was detected in perimedullary and pith tissue of all potato varieties, except for ‘Bora Valley’, which had purple flesh. Based on the varieties tested, purple skinned varieties showed higher antioxidant activity than red skinned tubers, while tubers with yellow skins showed the lowest antioxidant activity. The levels of three major phenolic compounds such as chlorogenic, caffeic, and ferulic acids were highly correlated to RC50 values for cortex tissue as well as whole tubers. Although chlorogenic, caffeic, and ferulic acids were the major phenolic acids contributing to the total antioxidant activity of potato tubers, caffeic acid was the most effective compound contributing to the total tuber antioxidant activity based on RC?? values.
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