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심재훈,Stephen G. Withers 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1
To achieve high level expression of β-glucosidase from Agrobacterium sp. (Abg) in an Escherichia coli expression system, 6 rare codons at +3, +11, +158, +308,+314, and +318 of abg gene were replaced with favored codons using site-directed mutagenesis. The rare codon replacements of +3 (CCC) and +11 (CCC) positions enhanced the expression level of Abg by 2- and 3.6-fold,respectively. The double mutant, Abg-DM, where both of +3 and +11 rare codon positions were modified showed a 5.2-fold higher expression level than the original abg gene. Circular dichroism (CD) spectra of the over-expressed Abg and original Abg enzymes were identical, indicating that Abg was properly folded during over-expression.
Kim , Jin Hyo,Ryu, Hyung Won,Shim, Jae Hoon,Park, Ki Hun,Withers, Stephen G. WILEY-VCH Verlag 2009 Chembiochem Vol.10 No.15
<P>A series of sulfonamide-containing hydroxylated chalcone (4–7) and quinolinone (8, 9) derivatives was synthesised and tested for inhibition of the trans-sialidase from Trypanosoma cruzi (TcTS). IC<SUB>50</SUB> values for these inhibitors ranged from 0.6 to 7.3 μM, with the dihydroxylated (catechol) derivatives being the tightest binders. Full kinetic analyses of inhibition were performed for these catechol derivatives, both for the transglycosylation reaction in the presence of lactose and for the hydrolysis reaction in its absence. Competitive inhibition was seen in each case with K<SUB>i</SUB> values for 5, 7 and 9 of 2.0, 2.2 and 0.2 μM, respectively, in the absence of lactose, and 4.6, 3.7 and 0.4 μM in its presence. None of the compounds tested showed any significant inhibition of the human sialidase Neu2, at concentrations up to 200 μM.</P> <B>Graphic Abstract</B> <P>Killer catechols: A series of sulfonamide-containing hydroxylated chalcones and quinolinone derivatives was synthesized and tested as inhibitors of the trans-sialidase from T. cruzi. Full kinetic analyses of inhibition were performed for these catechol derivatives, both for the transglycosylation reaction in the presence of lactose and for the hydrolysis reaction in its absence. K<SUB>i</SUB> values in the low and submicromolar range were found. <img src='wiley_img/14394227-2009-10-15-CBIC200900108-content.gif' alt='wiley_img/14394227-2009-10-15-CBIC200900108-content'> </P>